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The map label for this gene is metF [H]

Identifier: 50085383

GI number: 50085383

Start: 2254505

End: 2255341

Strand: Direct

Name: metF [H]

Synonym: ACIAD2283

Alternate gene names: 50085383

Gene position: 2254505-2255341 (Clockwise)

Preceding gene: 50085382

Following gene: 50085384

Centisome position: 62.65

GC content: 40.98

Gene sequence:

>837_bases
ATGTCAAAACGTACACCTATTTCATTTGAGTTTTTTCCTCCAAAAACAGATGCAGGTGCTGAAAAATTACGCACTGTACA
TCAAGAACTCCAACGCTTAAATCCTGAGTTTTTCTCGATTACTTACGGTGCAGGTGGTTCTACACGTGAGCGTACATTAG
CAGCTATCAATGACTTCAATGGTCAAGGTACTCCTGTCGCACCACATTTGTCATGTATTGGTGATAGCAAACTACGTATT
GCAGAATTACTTGATCTTTATAAGCGCCAAGGGATTGATCGTATTGTGGCTTTACGTGGTGATTTGCCTTCTGGTCAAGT
CGGATTAGGTGAACTGCCATATGCACAGGATCTGGTTCGTTTTATTCGGGAACATTCTGGCGATCATTTTCAGATTGAAG
TCGCAGCTTATCCTGAAATGCATCCACAAGCTGAAAACTTCGATGCAGATATCAAGCGTTTTATTGAAAAAGTGAACGCT
GGTGCCAATGCTGCCATTACCCAGTTTTTTTTCAATCCTGATTCGTATTTCTATTTTGTAGAACGAATTCAAAAAGAAGG
GATCCATATTCCCGTTGCACCAGGTATCATGCCAATCACCAATGCCAGTAATCTGATTCGTTTTGCAGATGGCACAGGGG
CTGAAATTCCACGCTGGATACGCAAGCAACTACAAGCTTATGGTGATGATACTCAAAGTATCAAAGCATTTGGTCATGAG
GTTATTTTAAAGCTTTGTGAGCGACTGATTGATGGTGGTGCTCCTAGCCTGCATTTCTATTCAATGAATAACACAGAACC
AACTCGTCAACTTGTGATTGACTTAGGTTTGAATTAA

Upstream 100 bases:

>100_bases
ATTTAAATCTGACGCTTACAAATACTAAGATATGAAGGGCAGCGCTGTAATAGGCTGCCCTTTTCTTAAACATTTTAAGT
GATTTTGAAAAGGATTTATA

Downstream 100 bases:

>100_bases
TTTTTAAAAGAGCAATGCCCCAATGAATCGTTTTTATATTGAAGCCGATCTAAGTGTAAACACAGATACTCTCTTAACTG
AATCTGTGTTTCATCATTGG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSKRTPISFEFFPPKTDAGAEKLRTVHQELQRLNPEFFSITYGAGGSTRERTLAAINDFNGQGTPVAPHLSCIGDSKLRI
AELLDLYKRQGIDRIVALRGDLPSGQVGLGELPYAQDLVRFIREHSGDHFQIEVAAYPEMHPQAENFDADIKRFIEKVNA
GANAAITQFFFNPDSYFYFVERIQKEGIHIPVAPGIMPITNASNLIRFADGTGAEIPRWIRKQLQAYGDDTQSIKAFGHE
VILKLCERLIDGGAPSLHFYSMNNTEPTRQLVIDLGLN

Sequences:

>Translated_278_residues
MSKRTPISFEFFPPKTDAGAEKLRTVHQELQRLNPEFFSITYGAGGSTRERTLAAINDFNGQGTPVAPHLSCIGDSKLRI
AELLDLYKRQGIDRIVALRGDLPSGQVGLGELPYAQDLVRFIREHSGDHFQIEVAAYPEMHPQAENFDADIKRFIEKVNA
GANAAITQFFFNPDSYFYFVERIQKEGIHIPVAPGIMPITNASNLIRFADGTGAEIPRWIRKQLQAYGDDTQSIKAFGHE
VILKLCERLIDGGAPSLHFYSMNNTEPTRQLVIDLGLN
>Mature_277_residues
SKRTPISFEFFPPKTDAGAEKLRTVHQELQRLNPEFFSITYGAGGSTRERTLAAINDFNGQGTPVAPHLSCIGDSKLRIA
ELLDLYKRQGIDRIVALRGDLPSGQVGLGELPYAQDLVRFIREHSGDHFQIEVAAYPEMHPQAENFDADIKRFIEKVNAG
ANAAITQFFFNPDSYFYFVERIQKEGIHIPVAPGIMPITNASNLIRFADGTGAEIPRWIRKQLQAYGDDTQSIKAFGHEV
ILKLCERLIDGGAPSLHFYSMNNTEPTRQLVIDLGLN

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=279, Percent_Identity=35.84229390681, Blast_Score=164, Evalue=6e-41,
Organism=Escherichia coli, GI1790377, Length=272, Percent_Identity=39.3382352941176, Blast_Score=196, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI25147441, Length=285, Percent_Identity=33.6842105263158, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI25147436, Length=285, Percent_Identity=33.6842105263158, Blast_Score=160, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6325234, Length=290, Percent_Identity=36.2068965517241, Blast_Score=147, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6321313, Length=272, Percent_Identity=31.9852941176471, Blast_Score=145, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 31062; Mature: 30930

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRTPISFEFFPPKTDAGAEKLRTVHQELQRLNPEFFSITYGAGGSTRERTLAAINDFN
CCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCC
GQGTPVAPHLSCIGDSKLRIAELLDLYKRQGIDRIVALRGDLPSGQVGLGELPYAQDLVR
CCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHH
FIREHSGDHFQIEVAAYPEMHPQAENFDADIKRFIEKVNAGANAAITQFFFNPDSYFYFV
HHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHH
ERIQKEGIHIPVAPGIMPITNASNLIRFADGTGAEIPRWIRKQLQAYGDDTQSIKAFGHE
HHHHHCCCEEECCCCCEEECCCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
VILKLCERLIDGGAPSLHFYSMNNTEPTRQLVIDLGLN
HHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCC
>Mature Secondary Structure 
SKRTPISFEFFPPKTDAGAEKLRTVHQELQRLNPEFFSITYGAGGSTRERTLAAINDFN
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCC
GQGTPVAPHLSCIGDSKLRIAELLDLYKRQGIDRIVALRGDLPSGQVGLGELPYAQDLVR
CCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHH
FIREHSGDHFQIEVAAYPEMHPQAENFDADIKRFIEKVNAGANAAITQFFFNPDSYFYFV
HHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHH
ERIQKEGIHIPVAPGIMPITNASNLIRFADGTGAEIPRWIRKQLQAYGDDTQSIKAFGHE
HHHHHCCCEEECCCCCEEECCCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
VILKLCERLIDGGAPSLHFYSMNNTEPTRQLVIDLGLN
HHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]