Click here to switch to the map view.

The map label for this gene is mltB [H]

Identifier: 50085367

GI number: 50085367

Start: 2235034

End: 2236035

Strand: Direct

Name: mltB [H]

Synonym: ACIAD2264

Alternate gene names: 50085367

Gene position: 2235034-2236035 (Clockwise)

Preceding gene: 50085366

Following gene: 50085368

Centisome position: 62.11

GC content: 40.12

Gene sequence:

>1002_bases
ATGTTGAATTTGGTGCTAAACAAACCATTTAAACAACTTCTAATCATGAGCAGTTTATTTATCACCGCAAGCTGTTCACA
AGCAAATGATTTTATTACAGATCCTAATTATCAAAGCTTTAAACAAAAAACCATGAATACCTATGGATTGACATCCGATC
AGATTGATAATGCGATGTCTGGTGCTAAAAATTTACCGAATATTTTAAGTATTATGACACGCCCTGGCGAAAGTAAACCT
TGGTACGATTATAAAGCCATGTTTCTCGCAGAAGGAACCATCCAACGTGGCGTAAGATTCAAAAACCAATATGCTGATGT
ACTTAATCGTGCAGAACAACAATTTGGTGTTCCCCAATCTATTATTCTGGGTATTTTAGGCGTAGAGACTGGTTATGGTG
CCAACAAAGGATCTTTTATCACACGAGACGCGCTTGCAACCTTGGCTTTTGGTTATCCTCGTCGAGCAGATTATTTCAGT
GATGAATTAGGCGCCCTGATTGCCTGGACGTACAAGGAAGGCTATCCAACCAACAGTATTGTTGGCTCTTATGCAGGTGC
AATTGGCTATCCACAATTCATGCCAAGCAACATTACCAAATTTGGTGTGGATTACGATGGAAGCGGACATATTGATCTCA
GAAATTCTGCTGAAGATGCAATTGCATCGATTGCAAATTATCTTGCACAAAATGGCTGGCAACGTAACCAACCAGTCGGA
TTTCCAGCCCGTTATAGCGGAAATACTCCTGATGCAGTGATTGCCAAAGACTTAACTCAGCCTACCCCCTACGGCGTACT
CAAATCTCAGGGAGTCACACCGCTTAATCCTGTAGTTAAAATTGATGATTTGGATCTGGTTAATGTAATCCAACTTCAAG
AAAATTATGGTCCTATGTACTATATTACCTATCCAAATTTTCAGGTAATTACCACTTACAACAGAAGTCGAATGTACGCT
ACAGCGGTATGGTTATTAGGCACAGAAGTCGCAAGTAGATAG

Upstream 100 bases:

>100_bases
ACTGCAATCATTACTTTAATGGCTACTTTTGGATTGGTCATGTCAATTCATACACATCGATAATTATATTTTTATTTTTG
ATTCAGGGACTAATAACATT

Downstream 100 bases:

>100_bases
CCTAAAAATTCACACAAATATAGAATGTCAATAAATATTTTTGTAAAAAAGTATATAAAACACCCATTTTAAATAATATG
TTACAATATTGGTTATTTTT

Product: membrane-bound lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MLNLVLNKPFKQLLIMSSLFITASCSQANDFITDPNYQSFKQKTMNTYGLTSDQIDNAMSGAKNLPNILSIMTRPGESKP
WYDYKAMFLAEGTIQRGVRFKNQYADVLNRAEQQFGVPQSIILGILGVETGYGANKGSFITRDALATLAFGYPRRADYFS
DELGALIAWTYKEGYPTNSIVGSYAGAIGYPQFMPSNITKFGVDYDGSGHIDLRNSAEDAIASIANYLAQNGWQRNQPVG
FPARYSGNTPDAVIAKDLTQPTPYGVLKSQGVTPLNPVVKIDDLDLVNVIQLQENYGPMYYITYPNFQVITTYNRSRMYA
TAVWLLGTEVASR

Sequences:

>Translated_333_residues
MLNLVLNKPFKQLLIMSSLFITASCSQANDFITDPNYQSFKQKTMNTYGLTSDQIDNAMSGAKNLPNILSIMTRPGESKP
WYDYKAMFLAEGTIQRGVRFKNQYADVLNRAEQQFGVPQSIILGILGVETGYGANKGSFITRDALATLAFGYPRRADYFS
DELGALIAWTYKEGYPTNSIVGSYAGAIGYPQFMPSNITKFGVDYDGSGHIDLRNSAEDAIASIANYLAQNGWQRNQPVG
FPARYSGNTPDAVIAKDLTQPTPYGVLKSQGVTPLNPVVKIDDLDLVNVIQLQENYGPMYYITYPNFQVITTYNRSRMYA
TAVWLLGTEVASR
>Mature_333_residues
MLNLVLNKPFKQLLIMSSLFITASCSQANDFITDPNYQSFKQKTMNTYGLTSDQIDNAMSGAKNLPNILSIMTRPGESKP
WYDYKAMFLAEGTIQRGVRFKNQYADVLNRAEQQFGVPQSIILGILGVETGYGANKGSFITRDALATLAFGYPRRADYFS
DELGALIAWTYKEGYPTNSIVGSYAGAIGYPQFMPSNITKFGVDYDGSGHIDLRNSAEDAIASIANYLAQNGWQRNQPVG
FPARYSGNTPDAVIAKDLTQPTPYGVLKSQGVTPLNPVVKIDDLDLVNVIQLQENYGPMYYITYPNFQVITTYNRSRMYA
TAVWLLGTEVASR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=312, Percent_Identity=39.1025641025641, Blast_Score=201, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 36910; Mature: 36910

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLVLNKPFKQLLIMSSLFITASCSQANDFITDPNYQSFKQKTMNTYGLTSDQIDNAMS
CCEEEECCHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
GAKNLPNILSIMTRPGESKPWYDYKAMFLAEGTIQRGVRFKNQYADVLNRAEQQFGVPQS
HHCCCHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IILGILGVETGYGANKGSFITRDALATLAFGYPRRADYFSDELGALIAWTYKEGYPTNSI
HHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCHHHHHHCEEEEEEECCCCCCHHH
VGSYAGAIGYPQFMPSNITKFGVDYDGSGHIDLRNSAEDAIASIANYLAQNGWQRNQPVG
HHHHHHHCCCCCCCCCCCEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCC
FPARYSGNTPDAVIAKDLTQPTPYGVLKSQGVTPLNPVVKIDDLDLVNVIQLQENYGPMY
CCCCCCCCCCCHHHHHHCCCCCCCCHHHCCCCCCCCCEEEECCCCHHHHEEECCCCCCEE
YITYPNFQVITTYNRSRMYATAVWLLGTEVASR
EEECCCEEEEEEECCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLNLVLNKPFKQLLIMSSLFITASCSQANDFITDPNYQSFKQKTMNTYGLTSDQIDNAMS
CCEEEECCHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
GAKNLPNILSIMTRPGESKPWYDYKAMFLAEGTIQRGVRFKNQYADVLNRAEQQFGVPQS
HHCCCHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IILGILGVETGYGANKGSFITRDALATLAFGYPRRADYFSDELGALIAWTYKEGYPTNSI
HHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCHHHHHHCEEEEEEECCCCCCHHH
VGSYAGAIGYPQFMPSNITKFGVDYDGSGHIDLRNSAEDAIASIANYLAQNGWQRNQPVG
HHHHHHHCCCCCCCCCCCEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCC
FPARYSGNTPDAVIAKDLTQPTPYGVLKSQGVTPLNPVVKIDDLDLVNVIQLQENYGPMY
CCCCCCCCCCCHHHHHHCCCCCCCCHHHCCCCCCCCCEEEECCCCHHHHEEECCCCCCEE
YITYPNFQVITTYNRSRMYATAVWLLGTEVASR
EEECCCEEEEEEECCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]