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The map label for this gene is fadI [H]

Identifier: 50085114

GI number: 50085114

Start: 1966552

End: 1967832

Strand: Direct

Name: fadI [H]

Synonym: ACIAD1977

Alternate gene names: 50085114

Gene position: 1966552-1967832 (Clockwise)

Preceding gene: 50085110

Following gene: 50085116

Centisome position: 54.65

GC content: 43.72

Gene sequence:

>1281_bases
ATGAGTCAAAATACTACTCGTCGTGTCGCCATCATTGGTGGTAATCGTATTCCGTTTGCCCGTTCTAATGGTGCGTATTT
TAAAGCCTCAAACATGGATATGCTCACCGCAGCACTGAATGGACTGGTTGAGCGTTATAATTTACAAGACAAACGTCTTG
GCGAAGTTGTTGCTGGCGCTGTGCTTAAACACAGCCGTGATTTTAATATGACTCGCGAGTGTGTTTTAAACACCAAACTT
GCTCCTGAAACCCCAGCTTATGACTTACAACAGGCTTGTGGAACAGGTTTACAAGCAGCGTTTGCCGTTGCGAATAAAAT
TGCACTAGGTCAAATTGATGTAGGTATAGCAGGTGGTGTAGATACCACATCTGATGCCCCGATTGCTTTTGGTGACGGTC
TGCGTAAAGCATTACTTGAGTTGAATATTGCCAAAACAGGTAAAGACCGTTTAAAAGCATTAACAAAAATTAATCTTAAA
GACCTGATGGATGCGCCTAAAAATGGCGAACCACGTACTGGGCTTTCAATGGGTGATCATGCTGCAATTACCGCACTTGA
GTGGAATATTGGTCGTAAAGAACAAGATGAACTGGCTGCTTCTTCTCACCACAAAATGGCAAAAGCCTACGAAGAAGGTT
TCTTCAATGATTTGATTACACCTTTTATGGGACTAGAACGGGATAACAATTTACGTCCTGATTCTTCAGTCGAAAAACTT
GCCAAGCTTAAGCCTGTATTTGGTAAAGGCGAAAATGCAACAATGACTGCTGGCAACTCGACTCCACTCACTGATGGTGC
ATCAGTTGTACTCCTTGCATCTGAAGAATGGGCAAAAGCGAATGGTCATGAGGTTCTGGCTTATTTAAGTTTCTCTGAAA
CCGCGGCTGTCGACTTTGTTGGTAAAAAAGAAGGCTTACTCATGGCTCCAGCGTATGCGGTTCCTCGTATGCTTGAGCGC
GCTGGCCTTAAATTACAGGATTTTGATTATTATGAAATCCATGAAGCTTTTGCATCGCAAGTGCTTGCTACACTTAAGGC
CTGGGAAGATCCTAAATTCTGTAAAGAGCGCCTAGGTTTAGATGCACCTTTAGGTGCGATTGATCGCTCAAAACTTAATA
TTTATGGCTCATCTCTTGCTGCGGGTCATCCTTTTGCTGCAACTGGCGGTCGTATTCTTGCCACTGCTGCCAAAATCCTG
AATGCAAAAGGTTCAGGTCGTATTCTGGTTTCGATCTGTGCCGCTGGTGGTCAAGGCGTTGTCGCGATTGTAGAAAAATA
A

Upstream 100 bases:

>100_bases
TACCACTACACGTACAAAAACGACAGCAGCCAGTAAAACTTCAACTGTCAAAGCCAATACTCCAGCCAAAACTCAACCTT
CTGTTCAACAGGATAAAAAC

Downstream 100 bases:

>100_bases
GTTATTCAGTTCTGAAAATAAAAAACAGCGCTTTCAAAGCGCTGTTTTTTATGAATGACGCGGCGTATAACCTAAACGTT
TTTCAATCGTGTCATAACAC

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSQNTTRRVAIIGGNRIPFARSNGAYFKASNMDMLTAALNGLVERYNLQDKRLGEVVAGAVLKHSRDFNMTRECVLNTKL
APETPAYDLQQACGTGLQAAFAVANKIALGQIDVGIAGGVDTTSDAPIAFGDGLRKALLELNIAKTGKDRLKALTKINLK
DLMDAPKNGEPRTGLSMGDHAAITALEWNIGRKEQDELAASSHHKMAKAYEEGFFNDLITPFMGLERDNNLRPDSSVEKL
AKLKPVFGKGENATMTAGNSTPLTDGASVVLLASEEWAKANGHEVLAYLSFSETAAVDFVGKKEGLLMAPAYAVPRMLER
AGLKLQDFDYYEIHEAFASQVLATLKAWEDPKFCKERLGLDAPLGAIDRSKLNIYGSSLAAGHPFAATGGRILATAAKIL
NAKGSGRILVSICAAGGQGVVAIVEK

Sequences:

>Translated_426_residues
MSQNTTRRVAIIGGNRIPFARSNGAYFKASNMDMLTAALNGLVERYNLQDKRLGEVVAGAVLKHSRDFNMTRECVLNTKL
APETPAYDLQQACGTGLQAAFAVANKIALGQIDVGIAGGVDTTSDAPIAFGDGLRKALLELNIAKTGKDRLKALTKINLK
DLMDAPKNGEPRTGLSMGDHAAITALEWNIGRKEQDELAASSHHKMAKAYEEGFFNDLITPFMGLERDNNLRPDSSVEKL
AKLKPVFGKGENATMTAGNSTPLTDGASVVLLASEEWAKANGHEVLAYLSFSETAAVDFVGKKEGLLMAPAYAVPRMLER
AGLKLQDFDYYEIHEAFASQVLATLKAWEDPKFCKERLGLDAPLGAIDRSKLNIYGSSLAAGHPFAATGGRILATAAKIL
NAKGSGRILVSICAAGGQGVVAIVEK
>Mature_425_residues
SQNTTRRVAIIGGNRIPFARSNGAYFKASNMDMLTAALNGLVERYNLQDKRLGEVVAGAVLKHSRDFNMTRECVLNTKLA
PETPAYDLQQACGTGLQAAFAVANKIALGQIDVGIAGGVDTTSDAPIAFGDGLRKALLELNIAKTGKDRLKALTKINLKD
LMDAPKNGEPRTGLSMGDHAAITALEWNIGRKEQDELAASSHHKMAKAYEEGFFNDLITPFMGLERDNNLRPDSSVEKLA
KLKPVFGKGENATMTAGNSTPLTDGASVVLLASEEWAKANGHEVLAYLSFSETAAVDFVGKKEGLLMAPAYAVPRMLERA
GLKLQDFDYYEIHEAFASQVLATLKAWEDPKFCKERLGLDAPLGAIDRSKLNIYGSSLAAGHPFAATGGRILATAAKILN
AKGSGRILVSICAAGGQGVVAIVEK

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=431, Percent_Identity=38.5150812064965, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI4501853, Length=440, Percent_Identity=29.0909090909091, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI167614485, Length=434, Percent_Identity=27.8801843317972, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI148539872, Length=429, Percent_Identity=28.6713286713287, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI4557237, Length=437, Percent_Identity=27.6887871853547, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1788683, Length=411, Percent_Identity=38.4428223844282, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI87082165, Length=430, Percent_Identity=30.2325581395349, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1788554, Length=431, Percent_Identity=29.9303944315545, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1787663, Length=431, Percent_Identity=28.7703016241299, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI48994986, Length=432, Percent_Identity=28.4722222222222, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17551802, Length=425, Percent_Identity=36.7058823529412, Blast_Score=256, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI133906874, Length=428, Percent_Identity=28.9719626168224, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI25147385, Length=434, Percent_Identity=26.2672811059908, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17535921, Length=429, Percent_Identity=26.3403263403263, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322031, Length=434, Percent_Identity=32.258064516129, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6325229, Length=442, Percent_Identity=29.6380090497738, Blast_Score=157, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17137578, Length=432, Percent_Identity=38.1944444444444, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24655093, Length=426, Percent_Identity=28.169014084507, Blast_Score=151, Evalue=6e-37,
Organism=Drosophila melanogaster, GI17648125, Length=431, Percent_Identity=29.6983758700696, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24640423, Length=432, Percent_Identity=25.9259259259259, Blast_Score=132, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 45481; Mature: 45349

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNTTRRVAIIGGNRIPFARSNGAYFKASNMDMLTAALNGLVERYNLQDKRLGEVVAGA
CCCCCCEEEEEEECCCCCEECCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VLKHSRDFNMTRECVLNTKLAPETPAYDLQQACGTGLQAAFAVANKIALGQIDVGIAGGV
HHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHEECEEEEEEECCC
DTTSDAPIAFGDGLRKALLELNIAKTGKDRLKALTKINLKDLMDAPKNGEPRTGLSMGDH
CCCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCC
AAITALEWNIGRKEQDELAASSHHKMAKAYEEGFFNDLITPFMGLERDNNLRPDSSVEKL
EEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
AKLKPVFGKGENATMTAGNSTPLTDGASVVLLASEEWAKANGHEVLAYLSFSETAAVDFV
HHHCCCCCCCCCCEEECCCCCCCCCCCCEEEEECCHHCCCCCCEEEEEEECCCCCEEEEE
GKKEGLLMAPAYAVPRMLERAGLKLQDFDYYEIHEAFASQVLATLKAWEDPKFCKERLGL
CCCCCEEECCHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
DAPLGAIDRSKLNIYGSSLAAGHPFAATGGRILATAAKILNAKGSGRILVSICAAGGQGV
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCEE
VAIVEK
EEEEEC
>Mature Secondary Structure 
SQNTTRRVAIIGGNRIPFARSNGAYFKASNMDMLTAALNGLVERYNLQDKRLGEVVAGA
CCCCCEEEEEEECCCCCEECCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VLKHSRDFNMTRECVLNTKLAPETPAYDLQQACGTGLQAAFAVANKIALGQIDVGIAGGV
HHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHEECEEEEEEECCC
DTTSDAPIAFGDGLRKALLELNIAKTGKDRLKALTKINLKDLMDAPKNGEPRTGLSMGDH
CCCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCC
AAITALEWNIGRKEQDELAASSHHKMAKAYEEGFFNDLITPFMGLERDNNLRPDSSVEKL
EEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
AKLKPVFGKGENATMTAGNSTPLTDGASVVLLASEEWAKANGHEVLAYLSFSETAAVDFV
HHHCCCCCCCCCCEEECCCCCCCCCCCCEEEEECCHHCCCCCCEEEEEEECCCCCEEEEE
GKKEGLLMAPAYAVPRMLERAGLKLQDFDYYEIHEAFASQVLATLKAWEDPKFCKERLGL
CCCCCEEECCHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
DAPLGAIDRSKLNIYGSSLAAGHPFAATGGRILATAAKILNAKGSGRILVSICAAGGQGV
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCEE
VAIVEK
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA