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The map label for this gene is fadE25 [H]

Identifier: 50084968

GI number: 50084968

Start: 1828090

End: 1829238

Strand: Direct

Name: fadE25 [H]

Synonym: ACIAD1821

Alternate gene names: 50084968

Gene position: 1828090-1829238 (Clockwise)

Preceding gene: 50084967

Following gene: 50084970

Centisome position: 50.8

GC content: 47.08

Gene sequence:

>1149_bases
ATGACAAACTGGAATGCATTATCAGACGACGAGTTTCGTCAGATTTTTAGAGATTGGGTACTTGAAAATTGCCCAGAATC
GCTACGGTATATGCGCAAGCAGCGTCCACTTTATGAAGAGGTAAAGGAATGGTATCAGGCTTTGGCCAATAAAGGTTGGC
TTGCGCCAGTATGGCCTGTCGAATACGGTGGAATGGGTTTAAATCCTGCCAAACACATGATTTATGTCGAAGAATGGGGA
CGTCTTGGTTGCCCTCGTATCCCAGACCATGGAATCGGATTAACCGGCCCATTGCTGTTGCAGTACGGAACAGATGCGCA
AAAGCAATACTTTTTACCACGTATTTTGTCGGGTGAGGATATGTGGTGTCAGGGGTATTCAGAACCCAATGCTGGATCTG
ATCTGGCGAGCTTACGCACACATGCGGTTTTAGAGGGCGATGAGTTTGTCGTCAATGGACAAAAAATCTGGACCACATTG
GCCACCTGTGCCAATTGGATATTTTTGCTGGTTCGAACCGATCGAGAAGCCAAACCCCAGCGTGGCATTTCGGTCTTGCT
GGTCGATATGACAAGTCCTGGCGTAGATGTGCGACCAATCGCCAATTTACGTGGAGAAGAAGACTTTTGTGAGGTGTTCT
TTGACAATGTCCGTGTTCCTAAAGCAAATTTGGTGGGTGAGCTTAATCAGGGGTGGACGATTGCCAAATCAGTTTTGGGT
CATGAGCGAATTTTCTTGGGGGCGCCTACACGCGCTGAAATTGCATTAGGGCGTTTAGATAAGTTGGCGGCAAGTAAAAA
CGCCTTTGAAGATTCAGCCTTTTTGTCACGCTATGCACAGCTTCGTCTTGAGGTTTATGATCTCGGCTCAAGTTACGAAC
GTTATGCCAAGGTACTGCGAAATGGTGGAGAGTTAGGCGCAGATGTTTCGGTGCTCAAGATTGTGGCGACAGAGCTTTAT
CAGCGCATCACAGAGGAAATGCTTGCCCTCGCAGGTGAAGAAGCACGTTATAACGATGATATTCAAACCAGCTTAGGTGC
TGTCGATGCGATGAATCTGTTTCTAGACTCACGTGCACCTGCCATTTTTGGTGGATCCAACGAAATCCAGCGTAATATTT
TGGCCAAAGCCGTACTGCAATTGCCTTAA

Upstream 100 bases:

>100_bases
AGCAAAATCTCACGGTTGAAATGACTCGTCACGAAGATTCTAAAGAGGCGATGCGTGCGTTTTTAGAAAAACGTCAACCT
GTATTTAAGGATAGCGTGTA

Downstream 100 bases:

>100_bases
CCATTCATTCATGCCCATTCGCATCAGCGTATGGGCATGAAATGCTGATGTACTTTAGTGAGCCAGTTCGATTGGTTGAT
GAAAGCTGTCGCTCTTTTTG

Product: acyl-CoA dehydrogenase

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MTNWNALSDDEFRQIFRDWVLENCPESLRYMRKQRPLYEEVKEWYQALANKGWLAPVWPVEYGGMGLNPAKHMIYVEEWG
RLGCPRIPDHGIGLTGPLLLQYGTDAQKQYFLPRILSGEDMWCQGYSEPNAGSDLASLRTHAVLEGDEFVVNGQKIWTTL
ATCANWIFLLVRTDREAKPQRGISVLLVDMTSPGVDVRPIANLRGEEDFCEVFFDNVRVPKANLVGELNQGWTIAKSVLG
HERIFLGAPTRAEIALGRLDKLAASKNAFEDSAFLSRYAQLRLEVYDLGSSYERYAKVLRNGGELGADVSVLKIVATELY
QRITEEMLALAGEEARYNDDIQTSLGAVDAMNLFLDSRAPAIFGGSNEIQRNILAKAVLQLP

Sequences:

>Translated_382_residues
MTNWNALSDDEFRQIFRDWVLENCPESLRYMRKQRPLYEEVKEWYQALANKGWLAPVWPVEYGGMGLNPAKHMIYVEEWG
RLGCPRIPDHGIGLTGPLLLQYGTDAQKQYFLPRILSGEDMWCQGYSEPNAGSDLASLRTHAVLEGDEFVVNGQKIWTTL
ATCANWIFLLVRTDREAKPQRGISVLLVDMTSPGVDVRPIANLRGEEDFCEVFFDNVRVPKANLVGELNQGWTIAKSVLG
HERIFLGAPTRAEIALGRLDKLAASKNAFEDSAFLSRYAQLRLEVYDLGSSYERYAKVLRNGGELGADVSVLKIVATELY
QRITEEMLALAGEEARYNDDIQTSLGAVDAMNLFLDSRAPAIFGGSNEIQRNILAKAVLQLP
>Mature_381_residues
TNWNALSDDEFRQIFRDWVLENCPESLRYMRKQRPLYEEVKEWYQALANKGWLAPVWPVEYGGMGLNPAKHMIYVEEWGR
LGCPRIPDHGIGLTGPLLLQYGTDAQKQYFLPRILSGEDMWCQGYSEPNAGSDLASLRTHAVLEGDEFVVNGQKIWTTLA
TCANWIFLLVRTDREAKPQRGISVLLVDMTSPGVDVRPIANLRGEEDFCEVFFDNVRVPKANLVGELNQGWTIAKSVLGH
ERIFLGAPTRAEIALGRLDKLAASKNAFEDSAFLSRYAQLRLEVYDLGSSYERYAKVLRNGGELGADVSVLKIVATELYQ
RITEEMLALAGEEARYNDDIQTSLGAVDAMNLFLDSRAPAIFGGSNEIQRNILAKAVLQLP

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI226958412, Length=394, Percent_Identity=27.1573604060914, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI226958414, Length=357, Percent_Identity=28.0112044817927, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4557233, Length=348, Percent_Identity=28.735632183908, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI4557231, Length=356, Percent_Identity=26.685393258427, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI187960098, Length=356, Percent_Identity=26.685393258427, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI21361497, Length=361, Percent_Identity=27.1468144044321, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI4501859, Length=349, Percent_Identity=26.9340974212034, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI4501857, Length=392, Percent_Identity=23.9795918367347, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI48976061, Length=368, Percent_Identity=30.1630434782609, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI211971008, Length=368, Percent_Identity=30.1630434782609, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI76496475, Length=354, Percent_Identity=26.271186440678, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI4557235, Length=354, Percent_Identity=26.271186440678, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI7656849, Length=297, Percent_Identity=25.9259259259259, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI38505218, Length=292, Percent_Identity=25.6849315068493, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786223, Length=363, Percent_Identity=25.8953168044077, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI87081958, Length=374, Percent_Identity=21.9251336898396, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17538396, Length=357, Percent_Identity=26.0504201680672, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17551932, Length=348, Percent_Identity=28.1609195402299, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17508101, Length=364, Percent_Identity=26.9230769230769, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17570075, Length=391, Percent_Identity=27.1099744245524, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17505929, Length=354, Percent_Identity=26.8361581920904, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17534899, Length=376, Percent_Identity=25.7978723404255, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17506239, Length=249, Percent_Identity=29.718875502008, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI32563615, Length=194, Percent_Identity=33.5051546391753, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17569725, Length=351, Percent_Identity=25.0712250712251, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71990804, Length=201, Percent_Identity=28.3582089552239, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71985184, Length=354, Percent_Identity=26.271186440678, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71985192, Length=213, Percent_Identity=30.5164319248826, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71982178, Length=199, Percent_Identity=30.1507537688442, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17533517, Length=348, Percent_Identity=25.2873563218391, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17562446, Length=325, Percent_Identity=26.1538461538462, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21356377, Length=349, Percent_Identity=26.9340974212034, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24666513, Length=387, Percent_Identity=24.2894056847545, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI21355753, Length=248, Percent_Identity=29.0322580645161, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24660351, Length=334, Percent_Identity=28.7425149700599, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24646207, Length=371, Percent_Identity=25.8760107816712, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI281363737, Length=204, Percent_Identity=29.9019607843137, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 42924; Mature: 42793

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNWNALSDDEFRQIFRDWVLENCPESLRYMRKQRPLYEEVKEWYQALANKGWLAPVWPV
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCC
EYGGMGLNPAKHMIYVEEWGRLGCPRIPDHGIGLTGPLLLQYGTDAQKQYFLPRILSGED
CCCCCCCCHHHHEEEEECCCCCCCCCCCCCCCCCCCHHHEEECCCHHHHHHCHHHCCCCC
MWCQGYSEPNAGSDLASLRTHAVLEGDEFVVNGQKIWTTLATCANWIFLLVRTDREAKPQ
CEECCCCCCCCCCHHHHHHHHHEECCCEEEECCHHHHHHHHHHHHHEEEEEECCCCCCCC
RGISVLLVDMTSPGVDVRPIANLRGEEDFCEVFFDNVRVPKANLVGELNQGWTIAKSVLG
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHC
HERIFLGAPTRAEIALGRLDKLAASKNAFEDSAFLSRYAQLRLEVYDLGSSYERYAKVLR
CCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHH
NGGELGADVSVLKIVATELYQRITEEMLALAGEEARYNDDIQTSLGAVDAMNLFLDSRAP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
AIFGGSNEIQRNILAKAVLQLP
EEECCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNWNALSDDEFRQIFRDWVLENCPESLRYMRKQRPLYEEVKEWYQALANKGWLAPVWPV
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCC
EYGGMGLNPAKHMIYVEEWGRLGCPRIPDHGIGLTGPLLLQYGTDAQKQYFLPRILSGED
CCCCCCCCHHHHEEEEECCCCCCCCCCCCCCCCCCCHHHEEECCCHHHHHHCHHHCCCCC
MWCQGYSEPNAGSDLASLRTHAVLEGDEFVVNGQKIWTTLATCANWIFLLVRTDREAKPQ
CEECCCCCCCCCCHHHHHHHHHEECCCEEEECCHHHHHHHHHHHHHEEEEEECCCCCCCC
RGISVLLVDMTSPGVDVRPIANLRGEEDFCEVFFDNVRVPKANLVGELNQGWTIAKSVLG
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHC
HERIFLGAPTRAEIALGRLDKLAASKNAFEDSAFLSRYAQLRLEVYDLGSSYERYAKVLR
CCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHH
NGGELGADVSVLKIVATELYQRITEEMLALAGEEARYNDDIQTSLGAVDAMNLFLDSRAP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
AIFGGSNEIQRNILAKAVLQLP
EEECCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]