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The map label for this gene is dcaJ [H]

Identifier: 50084851

GI number: 50084851

Start: 1701485

End: 1702159

Strand: Direct

Name: dcaJ [H]

Synonym: ACIAD1696

Alternate gene names: 50084851

Gene position: 1701485-1702159 (Clockwise)

Preceding gene: 50084850

Following gene: 50084852

Centisome position: 47.28

GC content: 40.74

Gene sequence:

>675_bases
ATGACGATTCAGAAAAGAAGTCGAGAAGATATCGCAATCATGATAGCAAAGGATATTCCTGATGGTTCGTATGTCAATCT
TGGAATTGGATTACCAACCCATGTCGCAAAATACTTGCCAAAAGATAAGGAAATTTTTCTGCACTCAGAGAATGGTGTAC
TGGCATTTGGTCCACCTCCTGCTGAAGGTGAAGAAGATCAGGACTTAGTCAATGCAGGTAAAGAGCTGGTGACTTTACTA
TCAGGTGGATGTTTTATGCATCATGGAGATTCATTTGACATTATGCGTGGTGGACATCTGGATATTTGCGTTATTGGGGC
ATTTCAAGTCGCGCTCAATGGTGATTTGGCTAACTGGCATACAGGTAAAGACGATGATGTGCCTGCTGTTGGGGGAGCAA
TGGATTTGGCGGTTGGGGCCAAGCGTATTTTTGTGTATATGGAACATACCACCAAAAAAGGCGAACCTAAAATTGTTAAA
CATCTGACTTATCCAATCACTGGTGAGCAGTGTGTTGATCGTATTTATACAGATTTATGTACGATTGAGCTGAAAGATGG
ACAGGCATATGTGATTGAAATGGTCGACGGATTAGATTTTGATACTTTGCAAGCCTTAACTGAATGCCCATTAATTGATC
ACTGTACTTATTCATCGTTGATTCAGCTTAGATAA

Upstream 100 bases:

>100_bases
CTCAAGTCAATCAAACTGTTGAATTGGGTGATCTTGATCCCGAATGCATTATTACACCCGGGATATTTGTACAGCATGTG
GTGCGATTAGGAGATATAAA

Downstream 100 bases:

>100_bases
ATGATATGAATCAAAAAAACTTATTTTTTAGAGTTTTCAATATTTATGCGCTAAAAATTTAAGACATATTTTAAAGGAAT
AAAGATGAAAAAACTTATTT

Product: acyl-CoA-transferase subunit B

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MTIQKRSREDIAIMIAKDIPDGSYVNLGIGLPTHVAKYLPKDKEIFLHSENGVLAFGPPPAEGEEDQDLVNAGKELVTLL
SGGCFMHHGDSFDIMRGGHLDICVIGAFQVALNGDLANWHTGKDDDVPAVGGAMDLAVGAKRIFVYMEHTTKKGEPKIVK
HLTYPITGEQCVDRIYTDLCTIELKDGQAYVIEMVDGLDFDTLQALTECPLIDHCTYSSLIQLR

Sequences:

>Translated_224_residues
MTIQKRSREDIAIMIAKDIPDGSYVNLGIGLPTHVAKYLPKDKEIFLHSENGVLAFGPPPAEGEEDQDLVNAGKELVTLL
SGGCFMHHGDSFDIMRGGHLDICVIGAFQVALNGDLANWHTGKDDDVPAVGGAMDLAVGAKRIFVYMEHTTKKGEPKIVK
HLTYPITGEQCVDRIYTDLCTIELKDGQAYVIEMVDGLDFDTLQALTECPLIDHCTYSSLIQLR
>Mature_223_residues
TIQKRSREDIAIMIAKDIPDGSYVNLGIGLPTHVAKYLPKDKEIFLHSENGVLAFGPPPAEGEEDQDLVNAGKELVTLLS
GGCFMHHGDSFDIMRGGHLDICVIGAFQVALNGDLANWHTGKDDDVPAVGGAMDLAVGAKRIFVYMEHTTKKGEPKIVKH
LTYPITGEQCVDRIYTDLCTIELKDGQAYVIEMVDGLDFDTLQALTECPLIDHCTYSSLIQLR

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI11545841, Length=205, Percent_Identity=42.9268292682927, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI4557817, Length=205, Percent_Identity=44.390243902439, Blast_Score=164, Evalue=6e-41,
Organism=Escherichia coli, GI1788552, Length=202, Percent_Identity=41.5841584158416, Blast_Score=161, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17531517, Length=208, Percent_Identity=45.6730769230769, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI21358325, Length=202, Percent_Identity=40.5940594059406, Blast_Score=157, Evalue=8e-39,
Organism=Drosophila melanogaster, GI24655794, Length=202, Percent_Identity=40.5940594059406, Blast_Score=156, Evalue=9e-39,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 24544; Mature: 24413

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQKRSREDIAIMIAKDIPDGSYVNLGIGLPTHVAKYLPKDKEIFLHSENGVLAFGPPP
CCCCCCCCCCEEEEEEECCCCCCEEEEECCCHHHHHHHCCCCCEEEEECCCCEEEECCCC
AEGEEDQDLVNAGKELVTLLSGGCFMHHGDSFDIMRGGHLDICVIGAFQVALNGDLANWH
CCCCCHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCEEEEEEEEEEEEECCCCCCCC
TGKDDDVPAVGGAMDLAVGAKRIFVYMEHTTKKGEPKIVKHLTYPITGEQCVDRIYTDLC
CCCCCCCCCCCCHHHHHHCCEEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHEE
TIELKDGQAYVIEMVDGLDFDTLQALTECPLIDHCTYSSLIQLR
EEEECCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHEECC
>Mature Secondary Structure 
TIQKRSREDIAIMIAKDIPDGSYVNLGIGLPTHVAKYLPKDKEIFLHSENGVLAFGPPP
CCCCCCCCCEEEEEEECCCCCCEEEEECCCHHHHHHHCCCCCEEEEECCCCEEEECCCC
AEGEEDQDLVNAGKELVTLLSGGCFMHHGDSFDIMRGGHLDICVIGAFQVALNGDLANWH
CCCCCHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCEEEEEEEEEEEEECCCCCCCC
TGKDDDVPAVGGAMDLAVGAKRIFVYMEHTTKKGEPKIVKHLTYPITGEQCVDRIYTDLC
CCCCCCCCCCCCHHHHHHCCEEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHEE
TIELKDGQAYVIEMVDGLDFDTLQALTECPLIDHCTYSSLIQLR
EEEECCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8063101; 8125318 [H]