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The map label for this gene is dcaF [H]

Identifier: 50084844

GI number: 50084844

Start: 1693338

End: 1694543

Strand: Reverse

Name: dcaF [H]

Synonym: ACIAD1689

Alternate gene names: 50084844

Gene position: 1694543-1693338 (Counterclockwise)

Preceding gene: 50084845

Following gene: 50084843

Centisome position: 47.09

GC content: 43.95

Gene sequence:

>1206_bases
ATGTTAAATGCATATATTTATGATGGTTTACGTACGCCATTTGGTCGCCATGCAGGTGAACTTGCTTCAATCCGTCCCGA
TGACCTTGCAGGATTAGTGATTCAACGCTTGATTGAAAAAACAGGTGTAGCAGGTGCAGACATTGAAGATGTGATTTTTG
GTGATACCAATCAGGCAGGTGAAGACAGTCGAAATATAGCCCGTCATGCTGCATTATTGGCAGGTTTGCCTGTGACTGTA
CCGGGCCAGACTGTAAACCGATTATGTGCCAGTGGCTTAGCTGCAATTATTGATTCTGCTCGTGCAATCACATGCGGTGA
GGGTGATTTATATATTGCGGGTGGTGTTGAGAGTATGTCACGCGCACCATTTGTCATGGGTAAAGCTGAAAGTGCCTATA
GCCGAGATGCAAAAATTTATGATACCACCATTGGTACACGTTTTCCTAATAAGAAAATTGTAGCCCAATACGGCGGTCAT
TCAATGCCTGAAACAGGTGATAATGTCGCGGTTGAATATGGCATTAGCCGTGAACAAGCCGATTTATTTGCTGCGCAATC
ACAGGCAAAATATCAGAAAGCACTCGAAGAAGGTTTTTTTGCTGGCGAAATTACAGCAGTAGAAGTGTCTCAAGGAAAAA
AATTACCCCCTAAGCAAGTGACTGAAGATGAACATCCGCGTCCGAGCTCCACGCTTGAGGCTTTAAGCAAATTAAAGCCA
TTATTTGAAGGTGGTGTGGTGACTGCTGGCAATGCTTCGGGTATCAATGACGGTGCAGCTGCGTTATTAATTGGTTCAGA
AGTTGCTGGTCAAAAATATGGTTTAACCCCCATGGCAAAAATATTGTCTGCGGCAGCGGCTGGTGTTGAACCACGCATTA
TGGGTGCAGGGCCAATCGAAGCAATTAAAAAAGCTGTTGCGCGTGCAGGTTTGACACTAGATGATTTAGACATTATTGAA
ATTAATGAAGCTTTTGCCTCACAAGTTTTGTCTTGCCTAAAAGGTTTAGGTATCGATTTTAACGACCCACGTGTTAATCC
AAATGGTGGTGCTATTGCAGTGGGTCACCCTCTAGGTGCATCGGGTGCGCGTTTGGCACTGACAGTAGCGCGTGAATTAC
AACGTCGCAATAAAAAATATGCAGTTGTCAGCTTGTGTATTGGTGTTGGTCAAGGTTTAGCAATGGTCATTGAAAACGTT
TCATAA

Upstream 100 bases:

>100_bases
CCTCTGACCCACGTTACCGCCCTAGTCCATGGTTACAACGCCGTGTTGCGCTTAATCTTCCACTTACATTTACTACTTAA
TTTAAAAGGGAATTTAATTC

Downstream 100 bases:

>100_bases
CCTTTCTTGAAAATATCCATTCATCACAACAGCATTGTGATGAATGGATTCAATTTACAAAGCAAAATAAAATACCTTAT
CATCTGGGCTTATTCATTCA

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MLNAYIYDGLRTPFGRHAGELASIRPDDLAGLVIQRLIEKTGVAGADIEDVIFGDTNQAGEDSRNIARHAALLAGLPVTV
PGQTVNRLCASGLAAIIDSARAITCGEGDLYIAGGVESMSRAPFVMGKAESAYSRDAKIYDTTIGTRFPNKKIVAQYGGH
SMPETGDNVAVEYGISREQADLFAAQSQAKYQKALEEGFFAGEITAVEVSQGKKLPPKQVTEDEHPRPSSTLEALSKLKP
LFEGGVVTAGNASGINDGAAALLIGSEVAGQKYGLTPMAKILSAAAAGVEPRIMGAGPIEAIKKAVARAGLTLDDLDIIE
INEAFASQVLSCLKGLGIDFNDPRVNPNGGAIAVGHPLGASGARLALTVARELQRRNKKYAVVSLCIGVGQGLAMVIENV
S

Sequences:

>Translated_401_residues
MLNAYIYDGLRTPFGRHAGELASIRPDDLAGLVIQRLIEKTGVAGADIEDVIFGDTNQAGEDSRNIARHAALLAGLPVTV
PGQTVNRLCASGLAAIIDSARAITCGEGDLYIAGGVESMSRAPFVMGKAESAYSRDAKIYDTTIGTRFPNKKIVAQYGGH
SMPETGDNVAVEYGISREQADLFAAQSQAKYQKALEEGFFAGEITAVEVSQGKKLPPKQVTEDEHPRPSSTLEALSKLKP
LFEGGVVTAGNASGINDGAAALLIGSEVAGQKYGLTPMAKILSAAAAGVEPRIMGAGPIEAIKKAVARAGLTLDDLDIIE
INEAFASQVLSCLKGLGIDFNDPRVNPNGGAIAVGHPLGASGARLALTVARELQRRNKKYAVVSLCIGVGQGLAMVIENV
S
>Mature_401_residues
MLNAYIYDGLRTPFGRHAGELASIRPDDLAGLVIQRLIEKTGVAGADIEDVIFGDTNQAGEDSRNIARHAALLAGLPVTV
PGQTVNRLCASGLAAIIDSARAITCGEGDLYIAGGVESMSRAPFVMGKAESAYSRDAKIYDTTIGTRFPNKKIVAQYGGH
SMPETGDNVAVEYGISREQADLFAAQSQAKYQKALEEGFFAGEITAVEVSQGKKLPPKQVTEDEHPRPSSTLEALSKLKP
LFEGGVVTAGNASGINDGAAALLIGSEVAGQKYGLTPMAKILSAAAAGVEPRIMGAGPIEAIKKAVARAGLTLDDLDIIE
INEAFASQVLSCLKGLGIDFNDPRVNPNGGAIAVGHPLGASGARLALTVARELQRRNKKYAVVSLCIGVGQGLAMVIENV
S

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=400, Percent_Identity=40, Blast_Score=295, Evalue=7e-80,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=42.0654911838791, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI4501853, Length=407, Percent_Identity=40.7862407862408, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI4557237, Length=397, Percent_Identity=35.264483627204, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=32.0843091334895, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI194353979, Length=398, Percent_Identity=30.4020100502513, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1787663, Length=402, Percent_Identity=56.7164179104478, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1788554, Length=404, Percent_Identity=46.5346534653465, Blast_Score=298, Evalue=3e-82,
Organism=Escherichia coli, GI87082165, Length=400, Percent_Identity=41, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI48994986, Length=406, Percent_Identity=39.9014778325123, Blast_Score=272, Evalue=3e-74,
Organism=Escherichia coli, GI1788683, Length=424, Percent_Identity=32.311320754717, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI133906874, Length=402, Percent_Identity=39.3034825870647, Blast_Score=285, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI25147385, Length=399, Percent_Identity=33.5839598997494, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17551802, Length=431, Percent_Identity=34.1067285382831, Blast_Score=191, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI17535921, Length=406, Percent_Identity=32.7586206896552, Blast_Score=181, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI17535917, Length=401, Percent_Identity=30.6733167082294, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6322031, Length=359, Percent_Identity=39.5543175487465, Blast_Score=217, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6325229, Length=403, Percent_Identity=36.7245657568238, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17648125, Length=404, Percent_Identity=39.6039603960396, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24655093, Length=404, Percent_Identity=39.3564356435644, Blast_Score=271, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24640423, Length=392, Percent_Identity=35.2040816326531, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17137578, Length=427, Percent_Identity=32.7868852459016, Blast_Score=187, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41782; Mature: 41782

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNAYIYDGLRTPFGRHAGELASIRPDDLAGLVIQRLIEKTGVAGADIEDVIFGDTNQAG
CCCEEEECCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEECCCCCCC
EDSRNIARHAALLAGLPVTVPGQTVNRLCASGLAAIIDSARAITCGEGDLYIAGGVESMS
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCHHHHC
RAPFVMGKAESAYSRDAKIYDTTIGTRFPNKKIVAQYGGHSMPETGDNVAVEYGISREQA
CCCEEEECCCHHHCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCCCEEEEECCCHHHH
DLFAAQSQAKYQKALEEGFFAGEITAVEVSQGKKLPPKQVTEDEHPRPSSTLEALSKLKP
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LFEGGVVTAGNASGINDGAAALLIGSEVAGQKYGLTPMAKILSAAAAGVEPRIMGAGPIE
HHCCCEEECCCCCCCCCCCEEEEECCHHCCCCCCCCHHHHHHHHHHCCCCCEEECCCHHH
AIKKAVARAGLTLDDLDIIEINEAFASQVLSCLKGLGIDFNDPRVNPNGGAIAVGHPLGA
HHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCCC
SGARLALTVARELQRRNKKYAVVSLCIGVGQGLAMVIENVS
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure
MLNAYIYDGLRTPFGRHAGELASIRPDDLAGLVIQRLIEKTGVAGADIEDVIFGDTNQAG
CCCEEEECCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEECCCCCCC
EDSRNIARHAALLAGLPVTVPGQTVNRLCASGLAAIIDSARAITCGEGDLYIAGGVESMS
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCHHHHC
RAPFVMGKAESAYSRDAKIYDTTIGTRFPNKKIVAQYGGHSMPETGDNVAVEYGISREQA
CCCEEEECCCHHHCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCCCEEEEECCCHHHH
DLFAAQSQAKYQKALEEGFFAGEITAVEVSQGKKLPPKQVTEDEHPRPSSTLEALSKLKP
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LFEGGVVTAGNASGINDGAAALLIGSEVAGQKYGLTPMAKILSAAAAGVEPRIMGAGPIE
HHCCCEEECCCCCCCCCCCEEEEECCHHCCCCCCCCHHHHHHHHHHCCCCCEEECCCHHH
AIKKAVARAGLTLDDLDIIEINEAFASQVLSCLKGLGIDFNDPRVNPNGGAIAVGHPLGA
HHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCCC
SGARLALTVARELQRRNKKYAVVSLCIGVGQGLAMVIENVS
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 10766858 [H]