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The map label for this gene is pcaT [H]

Identifier: 50084808

GI number: 50084808

Start: 1649163

End: 1650458

Strand: Reverse

Name: pcaT [H]

Synonym: ACIAD1651

Alternate gene names: 50084808

Gene position: 1650458-1649163 (Counterclockwise)

Preceding gene: 50084809

Following gene: 50084807

Centisome position: 45.86

GC content: 40.35

Gene sequence:

>1296_bases
ATGAGTGAAGTGGTTTATACCAAAGAAGAGAAACGCAAGCGCGTTATGGGAATTGTCGGGGCATCCTCGGGTAATTTAGT
TGAATGGTTCGATTTTTATATTTATGCAGTTTTTGCGATGTATTTCCAGCATGCATTGACAGCACCAAATATGAGTTCTA
CTGCACAAGGTGTTTATGTTTGGGGTGTATTTGCAGCAAGTTTTTTCATGCGACCTATCGGTAGCTGGTTATTTGGAAGA
ATTGCAGATAAACATGGCCGCAAACAATCAATGGTTATCTCAATTAGCCTGATGGCTGTCAGTTCATTCCTGTTTGCTTT
TTTACCGACCTATGATCAGGTCGGAATGATTGCACCGTTCTTACTTTTAATGGTTCGTTTACTACAGGGATTATCTGTAG
GCGGCGAATATGGGGCTGTTGCAACCTATATGAGTGAGATTGCATTAAAAGGTCATCGTGGATTTTATGCATCTTTCCAA
TATGTCACACTCTCAGGCGGTCAGCTCCTTGCAAGCTTACTGGGTGTAATTTTGCTAGCGTTCATGAGTGAACAACAATT
GATGGAGGGCGGCTGGCGTATTCCTTTTGTGATTGGTGGTATCGCTGCCATTATTTCATTGCTTGCTCGTAGTAAACTCG
AAGAAACACTTTCACACGAAGATAGTGAGAAGAAAGAGTCTGGTTCTTTACGCGAACTCTTTAAAAAGCACTGGGGTACA
TTCCTGCTTGTTGTGGGTTATACGTCTGCAGGCTCCTTAACGTTCTATGTTCTTACTGTTTATTCAAAAACGTATCTCAC
CAATATCGGTATTGATGGCAAAGTCGTTGGTTATATGATGACCTTTGCACTATTTATTTACATGATTGCACAACCTCTTT
TTGGCGCAATTTCAGACCGAATTGGCCGACGTGCTTCCATGTTACTATTTAGTGGATTAAGCATTGTGTTTATCTATCCA
GTGATGGTGATTGGAATGCGACACTTTAGTTATTCTCCAGTGATTGTCACATTACTGCTTATCTTTTTGATGATGATTTT
GAGCTTCTATACCTCAATTAGTGGTCTAATCAAAGCTGAAATGTTTCCACCACATGTACGTGCCTTAGGCGTAGGTTTTT
CTTATGCAGTAGGTAATGCCATATTCGGTGGTTCTGCACCTGCTGTTGCATTGCAGTTCAAGCAAGCAGGAATTGAAAAT
GAATTCTTTATTTATGTGATTGTGATGCTTGTCATCTGTTTCTTCTGTAGTTTTAAACTGCCAAAAGAACCTGAATATCT
CCACCACGACAAATAA

Upstream 100 bases:

>100_bases
CTGTAGTTTTATTCATTTATTGCAATTTCTATATGTGGGCTAAAAGTCTTTTAGAGACTCAATTTAATAACATAACGAAA
CAAACCATAAGGACATTCAT

Downstream 100 bases:

>100_bases
TGATTTGAACTCTAAGATCCGCCAGACTGGCGGATTTTTTATAGGTCCAAGCTGGTTTTTATAGAACAAAAATTATTTAT
ACTTAATATAGTTTCAAAGG

Product: major facilitator superfamily alpha-ketoglutarate permease

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSEVVYTKEEKRKRVMGIVGASSGNLVEWFDFYIYAVFAMYFQHALTAPNMSSTAQGVYVWGVFAASFFMRPIGSWLFGR
IADKHGRKQSMVISISLMAVSSFLFAFLPTYDQVGMIAPFLLLMVRLLQGLSVGGEYGAVATYMSEIALKGHRGFYASFQ
YVTLSGGQLLASLLGVILLAFMSEQQLMEGGWRIPFVIGGIAAIISLLARSKLEETLSHEDSEKKESGSLRELFKKHWGT
FLLVVGYTSAGSLTFYVLTVYSKTYLTNIGIDGKVVGYMMTFALFIYMIAQPLFGAISDRIGRRASMLLFSGLSIVFIYP
VMVIGMRHFSYSPVIVTLLLIFLMMILSFYTSISGLIKAEMFPPHVRALGVGFSYAVGNAIFGGSAPAVALQFKQAGIEN
EFFIYVIVMLVICFFCSFKLPKEPEYLHHDK

Sequences:

>Translated_431_residues
MSEVVYTKEEKRKRVMGIVGASSGNLVEWFDFYIYAVFAMYFQHALTAPNMSSTAQGVYVWGVFAASFFMRPIGSWLFGR
IADKHGRKQSMVISISLMAVSSFLFAFLPTYDQVGMIAPFLLLMVRLLQGLSVGGEYGAVATYMSEIALKGHRGFYASFQ
YVTLSGGQLLASLLGVILLAFMSEQQLMEGGWRIPFVIGGIAAIISLLARSKLEETLSHEDSEKKESGSLRELFKKHWGT
FLLVVGYTSAGSLTFYVLTVYSKTYLTNIGIDGKVVGYMMTFALFIYMIAQPLFGAISDRIGRRASMLLFSGLSIVFIYP
VMVIGMRHFSYSPVIVTLLLIFLMMILSFYTSISGLIKAEMFPPHVRALGVGFSYAVGNAIFGGSAPAVALQFKQAGIEN
EFFIYVIVMLVICFFCSFKLPKEPEYLHHDK
>Mature_430_residues
SEVVYTKEEKRKRVMGIVGASSGNLVEWFDFYIYAVFAMYFQHALTAPNMSSTAQGVYVWGVFAASFFMRPIGSWLFGRI
ADKHGRKQSMVISISLMAVSSFLFAFLPTYDQVGMIAPFLLLMVRLLQGLSVGGEYGAVATYMSEIALKGHRGFYASFQY
VTLSGGQLLASLLGVILLAFMSEQQLMEGGWRIPFVIGGIAAIISLLARSKLEETLSHEDSEKKESGSLRELFKKHWGTF
LLVVGYTSAGSLTFYVLTVYSKTYLTNIGIDGKVVGYMMTFALFIYMIAQPLFGAISDRIGRRASMLLFSGLSIVFIYPV
MVIGMRHFSYSPVIVTLLLIFLMMILSFYTSISGLIKAEMFPPHVRALGVGFSYAVGNAIFGGSAPAVALQFKQAGIENE
FFIYVIVMLVICFFCSFKLPKEPEYLHHDK

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=418, Percent_Identity=53.11004784689, Blast_Score=403, Evalue=1e-114,
Organism=Escherichia coli, GI1790550, Length=408, Percent_Identity=31.3725490196078, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1789941, Length=405, Percent_Identity=31.8518518518519, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1788292, Length=379, Percent_Identity=25.8575197889182, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI87082404, Length=330, Percent_Identity=26.3636363636364, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87082231, Length=228, Percent_Identity=24.5614035087719, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47891; Mature: 47760

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVVYTKEEKRKRVMGIVGASSGNLVEWFDFYIYAVFAMYFQHALTAPNMSSTAQGVYV
CCCCEECHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
WGVFAASFFMRPIGSWLFGRIADKHGRKQSMVISISLMAVSSFLFAFLPTYDQVGMIAPF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHH
LLLMVRLLQGLSVGGEYGAVATYMSEIALKGHRGFYASFQYVTLSGGQLLASLLGVILLA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHHHHHH
FMSEQQLMEGGWRIPFVIGGIAAIISLLARSKLEETLSHEDSEKKESGSLRELFKKHWGT
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHCCC
FLLVVGYTSAGSLTFYVLTVYSKTYLTNIGIDGKVVGYMMTFALFIYMIAQPLFGAISDR
EEEEEEECCCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGRRASMLLFSGLSIVFIYPVMVIGMRHFSYSPVIVTLLLIFLMMILSFYTSISGLIKAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
MFPPHVRALGVGFSYAVGNAIFGGSAPAVALQFKQAGIENEFFIYVIVMLVICFFCSFKL
CCCCHHHHHHCCHHHHHCCHHCCCCCCHHEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
PKEPEYLHHDK
CCCCCCCCCCC
>Mature Secondary Structure 
SEVVYTKEEKRKRVMGIVGASSGNLVEWFDFYIYAVFAMYFQHALTAPNMSSTAQGVYV
CCCEECHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
WGVFAASFFMRPIGSWLFGRIADKHGRKQSMVISISLMAVSSFLFAFLPTYDQVGMIAPF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHH
LLLMVRLLQGLSVGGEYGAVATYMSEIALKGHRGFYASFQYVTLSGGQLLASLLGVILLA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHHHHHH
FMSEQQLMEGGWRIPFVIGGIAAIISLLARSKLEETLSHEDSEKKESGSLRELFKKHWGT
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHCCC
FLLVVGYTSAGSLTFYVLTVYSKTYLTNIGIDGKVVGYMMTFALFIYMIAQPLFGAISDR
EEEEEEECCCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGRRASMLLFSGLSIVFIYPVMVIGMRHFSYSPVIVTLLLIFLMMILSFYTSISGLIKAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
MFPPHVRALGVGFSYAVGNAIFGGSAPAVALQFKQAGIENEFFIYVIVMLVICFFCSFKL
CCCCHHHHHHCCHHHHHCCHHCCCCCCHHEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
PKEPEYLHHDK
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]