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The map label for this gene is cobB [H]

Identifier: 50084807

GI number: 50084807

Start: 1648340

End: 1649041

Strand: Reverse

Name: cobB [H]

Synonym: ACIAD1650

Alternate gene names: 50084807

Gene position: 1649041-1648340 (Counterclockwise)

Preceding gene: 50084808

Following gene: 50084806

Centisome position: 45.82

GC content: 40.6

Gene sequence:

>702_bases
ATGAAAAAGCTTGTGGTCTTCTCGGGTGCTGGCATGAGTGCAGAAAGTGGAATTCATACCTTTCGAGATCATGATGGTCT
TTGGGAAAATTATCGGATCGAAGAGGTCGCAACACCAGAAGCATGGCAGCAAAATCCATCCTTGGTACAGCATTTTTATA
ATGAACGCCGTAAAAACATTCTTGCGGCACAACCTAATCTCGCCCATCAAATCATTGCTCAACTTGAATCGTGTTATCAG
GTTCAAGTGATTACTCAAAATATAGATGATTTACATGAGCGTGCAGGTTCAACACATGTCTTACATTTACATGGCAATAT
CCGTCTAGCGAAGAGCTCAGGGCCAAACGCACAGTTTACACAAACGTTTTATCCTATTGACGGGTGGCAGCTTGATCTAA
ATCAAGATAAATGTAATGAAGGTTATCCTTTACGGCCACATGTGGTATGGTTTGGCGAAGCCGTACCAGCCTATGATGAT
GCCATCGCTATGTTAAAAGATGCTGATGTATTTATTGTAATTGGTTCCACGCTAAGTGTGTACCCTGTAGCAGGACTGAT
TCATGAAATCCCGAATCATTGTGAAGCCTATTATATCGATCCTCAAGCAGATTATTTTCGCGTTCCCCATCAATACACTT
TACTAAAAATGACAGCAACCGAGGGAATGAAACAACTTTATCTTAAGTTAAAAAATCTTTAA

Upstream 100 bases:

>100_bases
CCAGACTGGCGGATTTTTTATAGGTCCAAGCTGGTTTTTATAGAACAAAAATTATTTATACTTAATATAGTTTCAAAGGA
TAATGCATAAAGGATAATCA

Downstream 100 bases:

>100_bases
TACGCTCTTAAATATTTCGATAAAAATAATTTGGTTTATTTTCAAACGATCATTTGCATAGTCTTTGATCTACACAAACT
CACAAAAGAGATTATTTTTT

Product: cobalamin biosynthetic protein (CobB)

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MKKLVVFSGAGMSAESGIHTFRDHDGLWENYRIEEVATPEAWQQNPSLVQHFYNERRKNILAAQPNLAHQIIAQLESCYQ
VQVITQNIDDLHERAGSTHVLHLHGNIRLAKSSGPNAQFTQTFYPIDGWQLDLNQDKCNEGYPLRPHVVWFGEAVPAYDD
AIAMLKDADVFIVIGSTLSVYPVAGLIHEIPNHCEAYYIDPQADYFRVPHQYTLLKMTATEGMKQLYLKLKNL

Sequences:

>Translated_233_residues
MKKLVVFSGAGMSAESGIHTFRDHDGLWENYRIEEVATPEAWQQNPSLVQHFYNERRKNILAAQPNLAHQIIAQLESCYQ
VQVITQNIDDLHERAGSTHVLHLHGNIRLAKSSGPNAQFTQTFYPIDGWQLDLNQDKCNEGYPLRPHVVWFGEAVPAYDD
AIAMLKDADVFIVIGSTLSVYPVAGLIHEIPNHCEAYYIDPQADYFRVPHQYTLLKMTATEGMKQLYLKLKNL
>Mature_233_residues
MKKLVVFSGAGMSAESGIHTFRDHDGLWENYRIEEVATPEAWQQNPSLVQHFYNERRKNILAAQPNLAHQIIAQLESCYQ
VQVITQNIDDLHERAGSTHVLHLHGNIRLAKSSGPNAQFTQTFYPIDGWQLDLNQDKCNEGYPLRPHVVWFGEAVPAYDD
AIAMLKDADVFIVIGSTLSVYPVAGLIHEIPNHCEAYYIDPQADYFRVPHQYTLLKMTATEGMKQLYLKLKNL

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=190, Percent_Identity=37.8947368421053, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI6912664, Length=208, Percent_Identity=37.0192307692308, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI13787215, Length=208, Percent_Identity=37.0192307692308, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI7657575, Length=213, Percent_Identity=30.5164319248826, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI7706712, Length=216, Percent_Identity=29.1666666666667, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI308199517, Length=199, Percent_Identity=46.2311557788945, Blast_Score=170, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17541892, Length=200, Percent_Identity=29.5, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 26519; Mature: 26519

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVVFSGAGMSAESGIHTFRDHDGLWENYRIEEVATPEAWQQNPSLVQHFYNERRKNI
CCEEEEEECCCCCCCCCCCHHHCCCCCCCCEEEEECCCCHHHCCCHHHHHHHHHHHHCCE
LAAQPNLAHQIIAQLESCYQVQVITQNIDDLHERAGSTHVLHLHGNIRLAKSSGPNAQFT
EECCCCHHHHHHHHHHHHEEEEEECCCHHHHHHHCCCEEEEEEECCEEEEECCCCCCEEE
QTFYPIDGWQLDLNQDKCNEGYPLRPHVVWFGEAVPAYDDAIAMLKDADVFIVIGSTLSV
EEEECCCCEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHEECCCEEEEECCCCCH
YPVAGLIHEIPNHCEAYYIDPQADYFRVPHQYTLLKMTATEGMKQLYLKLKNL
HHHHHHHHHCCCCCEEEEECCCCCEEECCCEEEEEEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKLVVFSGAGMSAESGIHTFRDHDGLWENYRIEEVATPEAWQQNPSLVQHFYNERRKNI
CCEEEEEECCCCCCCCCCCHHHCCCCCCCCEEEEECCCCHHHCCCHHHHHHHHHHHHCCE
LAAQPNLAHQIIAQLESCYQVQVITQNIDDLHERAGSTHVLHLHGNIRLAKSSGPNAQFT
EECCCCHHHHHHHHHHHHEEEEEECCCHHHHHHHCCCEEEEEEECCEEEEECCCCCCEEE
QTFYPIDGWQLDLNQDKCNEGYPLRPHVVWFGEAVPAYDDAIAMLKDADVFIVIGSTLSV
EEEECCCCEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHEECCCEEEEECCCCCH
YPVAGLIHEIPNHCEAYYIDPQADYFRVPHQYTLLKMTATEGMKQLYLKLKNL
HHHHHHHHHCCCCCEEEEECCCCCEEECCCEEEEEEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]