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The map label for this gene is nthA [H]

Identifier: 50084777

GI number: 50084777

Start: 1614625

End: 1615224

Strand: Reverse

Name: nthA [H]

Synonym: ACIAD1616

Alternate gene names: 50084777

Gene position: 1615224-1614625 (Counterclockwise)

Preceding gene: 50084778

Following gene: 50084776

Centisome position: 44.88

GC content: 43.17

Gene sequence:

>600_bases
ATGAGTATATCTGTTTCTGAAACATCAAGCTCAACCCCAGGCGAGAGAGCATGGGCGTTACATAAGGTTCTTACCAATAA
AGGTTTAATTCCAGAGGGCTTTATTGAAGGTTTAACCGACCTTCTTGCCAATAAATTTGATCCAGCCAATGGTGCTCAAG
TTGTTGCCAAAGCATGGACCGATCCTGCTTATCGTGAACTTTTATTGCGTGACGGTACTGCGGCTTGTGAAGAATTTGGT
TTTACTGGCCCACAAGGTGAGTACATTGTCGCACTCGAAGATACTACAGATGTAAAAAATGTCATCGTTTGTAGCTTGTG
TTCATGTACAAATTGGCCTGTTCTAGGGCTTCCACCTGAATGGTACAAAAGCTTTGAATTCCGTGCACGCTTAGTTCGTG
AAGGTCGTACTGTTCTTAAAGAACTCGGAACAGAATTACCCGAAAATATGACCGTCAAAGTTTGGGATACCAGTGCAGAA
ACCCGCTATCTTGTTTTACCTATGCGTCCTGCTGGAACTGAAAATATGACCGTAGAGGAACTGCAAAAGCTGATTACCAA
AGACGTCCTCATTGGCGTTGCACTGCCAAAATTACCTTAA

Upstream 100 bases:

>100_bases
TTGGAAAACAATTTAATCAACAGCAAGATCACATCAAGGATTGCAACATTCTCTTTCTAAGTGAGTTGTTGTATTTCATC
TCTACCAAGGAGCGTTACAC

Downstream 100 bases:

>100_bases
TTCTTTTGTAAAAATATATCGTTACTTATATGGAGTAAAATTATGGATGGTTTTCATGATTTAGGTGGTGTACAAGGCTT
TGGTGCTATTGATCATAAGA

Product: nitrile hydratase subunit alpha (Nitrilase) (NHase)

Products: NA

Alternate protein names: NHase; Nitrilase [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MSISVSETSSSTPGERAWALHKVLTNKGLIPEGFIEGLTDLLANKFDPANGAQVVAKAWTDPAYRELLLRDGTAACEEFG
FTGPQGEYIVALEDTTDVKNVIVCSLCSCTNWPVLGLPPEWYKSFEFRARLVREGRTVLKELGTELPENMTVKVWDTSAE
TRYLVLPMRPAGTENMTVEELQKLITKDVLIGVALPKLP

Sequences:

>Translated_199_residues
MSISVSETSSSTPGERAWALHKVLTNKGLIPEGFIEGLTDLLANKFDPANGAQVVAKAWTDPAYRELLLRDGTAACEEFG
FTGPQGEYIVALEDTTDVKNVIVCSLCSCTNWPVLGLPPEWYKSFEFRARLVREGRTVLKELGTELPENMTVKVWDTSAE
TRYLVLPMRPAGTENMTVEELQKLITKDVLIGVALPKLP
>Mature_198_residues
SISVSETSSSTPGERAWALHKVLTNKGLIPEGFIEGLTDLLANKFDPANGAQVVAKAWTDPAYRELLLRDGTAACEEFGF
TGPQGEYIVALEDTTDVKNVIVCSLCSCTNWPVLGLPPEWYKSFEFRARLVREGRTVLKELGTELPENMTVKVWDTSAET
RYLVLPMRPAGTENMTVEELQKLITKDVLIGVALPKLP

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides. Industrial production of acrylamide is now being developed using some of the enzymes of this class [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit alpha family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004232
- InterPro:   IPR018141
- ProDom:   PD007559 [H]

Pfam domain/function: PF02979 NHase_alpha [H]

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 21943; Mature: 21812

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISVSETSSSTPGERAWALHKVLTNKGLIPEGFIEGLTDLLANKFDPANGAQVVAKAWT
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCC
DPAYRELLLRDGTAACEEFGFTGPQGEYIVALEDTTDVKNVIVCSLCSCTNWPVLGLPPE
CHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEECCCHH
WYKSFEFRARLVREGRTVLKELGTELPENMTVKVWDTSAETRYLVLPMRPAGTENMTVEE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCHHH
LQKLITKDVLIGVALPKLP
HHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SISVSETSSSTPGERAWALHKVLTNKGLIPEGFIEGLTDLLANKFDPANGAQVVAKAWT
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCC
DPAYRELLLRDGTAACEEFGFTGPQGEYIVALEDTTDVKNVIVCSLCSCTNWPVLGLPPE
CHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEECCCHH
WYKSFEFRARLVREGRTVLKELGTELPENMTVKVWDTSAETRYLVLPMRPAGTENMTVEE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCHHH
LQKLITKDVLIGVALPKLP
HHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2013568 [H]