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The map label for this gene is amdA

Identifier: 50084778

GI number: 50084778

Start: 1615309

End: 1616823

Strand: Reverse

Name: amdA

Synonym: ACIAD1618

Alternate gene names: NA

Gene position: 1616823-1615309 (Counterclockwise)

Preceding gene: 50084779

Following gene: 50084777

Centisome position: 44.93

GC content: 41.78

Gene sequence:

>1515_bases
ATGGCAATTCAACGACCAACAATTGATGTTTTAAAACAGATCGCAAATGATCTACATATACAGTTGACTGATACAGAAGC
CGCAGAATACTTAAGTATTATACAAAGCAATTTCGATGCCTATGATCTCATTGATGAACTAGATGATGAGATTCCAGAAG
TTCGTTATCCTCGCGCGAATAGTTATCGTCCTGATGCAACAGAAAATCCTTTAAATGCATGGTATTACAAAACTGAAGTA
AAAGGCGCCCTATCTGGAAAACTTTCAGGAAAGACCATTGCTCTGAAAGATAATATTTTTCTTGCTGATGTTCCAATGAT
GAATGGTTCAACAACGCTAGAAGGCTTTGTACCTGAGTATGATGCAACCATTGTCACCCGAATGTTAGATGCTGGTGCCA
CCATTTTAGGCAAAGCCACATGCGAACATTTCTGTCTCTCTGGTGGCAGTCATACTTCTGACCCTGTTGCTGTCCATAAT
CCTTATCGACATGGATATTCGGCAGGAGGTTCCTCCTCTGGAAGCGCTGCTTTGGTTTCAAATGGTGACGTCGATTTAGC
AATTGCATGTGATCAGGGAGGCTCTATCCGTATTCCTGCTGCTTTTTGCGGTATCTACGGTATGAAGCCAACGCATGGTC
TCGTTCCATATACAGGGATTATGCCTATTGAAAATACCATCGACCATGTGGGCCCAGTGAGTCGTACCGTTCGAGACAAT
GCATTATTACTTGAAGTAATTGCAGGTGGTGATGGGCTAGATCCACGTCAATATCATGTCAAAACAGAAGACTATACAAG
TTATCTGGATCGCGGTGTAAAAGGATTGAAAATCGGAGTTGTTCGCGAGGGTTTTAATTTAGCCAATATGGATCCTAAAG
TTGCTGAAAAAGTCCGTAAAGCAATCGTCGAATTTGAAAAAATGGGGGCAATTGTTGAAGACATTTCTCTGCCTGAACAT
GCTCTGGCTGGTGCTCTCTGGTCACCTGTTGGCTGTGAAGGTTTAACCGCACAAATGATGCATGGCAATGGAATGGGATT
TAACTGGGAAGGTTTATATGGTGTCAAACTTATTGATAAACATAAAGATTGGCGCAAACAAGCAGATCAACTTTCACCTA
CCTTAAAAGCATGTATGTTTGTAGGCGAATATGGAATCAAAAATTATTCTGGTCGTTACTACGCAAAATCAAAAAATATC
ACGAGACGTGCTCGAGCTGCGTATGACCAGAAACTGGCTGAATATGATCTATTGTTAATGCCAACCGTCCCTATGGTTGC
ACAGCCTCTTCCAGAACCAGGCTGTTCTCTCACTGAATATATTAGCAAAGCATTTGAAATGATTGGCAATACGGCGTCAC
AGGATATTACAGGCCACCCTGCCATGAGTATTCCGTGTGGATTAGTCGATGATTTACCTGTAGGTCTGATGCTCATTGGA
CCTTATTTTGGCGAGGGCAAAATATATCAGGCAGCAGCAGCATTCGAAAAAAATGTCGATTGGAAAACAATTTAA

Upstream 100 bases:

>100_bases
TATAAACAAATGTCTGGCGTTTTAAATCAAGTTTTCAAGAAAAGAACAGACTAATTTAAAGCATATCTTCAGTCAGAATG
ATCAGTGGACGGGTAAAAAC

Downstream 100 bases:

>100_bases
TCAACAGCAAGATCACATCAAGGATTGCAACATTCTCTTTCTAAGTGAGTTGTTGTATTTCATCTCTACCAAGGAGCGTT
ACACATGAGTATATCTGTTT

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MAIQRPTIDVLKQIANDLHIQLTDTEAAEYLSIIQSNFDAYDLIDELDDEIPEVRYPRANSYRPDATENPLNAWYYKTEV
KGALSGKLSGKTIALKDNIFLADVPMMNGSTTLEGFVPEYDATIVTRMLDAGATILGKATCEHFCLSGGSHTSDPVAVHN
PYRHGYSAGGSSSGSAALVSNGDVDLAIACDQGGSIRIPAAFCGIYGMKPTHGLVPYTGIMPIENTIDHVGPVSRTVRDN
ALLLEVIAGGDGLDPRQYHVKTEDYTSYLDRGVKGLKIGVVREGFNLANMDPKVAEKVRKAIVEFEKMGAIVEDISLPEH
ALAGALWSPVGCEGLTAQMMHGNGMGFNWEGLYGVKLIDKHKDWRKQADQLSPTLKACMFVGEYGIKNYSGRYYAKSKNI
TRRARAAYDQKLAEYDLLLMPTVPMVAQPLPEPGCSLTEYISKAFEMIGNTASQDITGHPAMSIPCGLVDDLPVGLMLIG
PYFGEGKIYQAAAAFEKNVDWKTI

Sequences:

>Translated_504_residues
MAIQRPTIDVLKQIANDLHIQLTDTEAAEYLSIIQSNFDAYDLIDELDDEIPEVRYPRANSYRPDATENPLNAWYYKTEV
KGALSGKLSGKTIALKDNIFLADVPMMNGSTTLEGFVPEYDATIVTRMLDAGATILGKATCEHFCLSGGSHTSDPVAVHN
PYRHGYSAGGSSSGSAALVSNGDVDLAIACDQGGSIRIPAAFCGIYGMKPTHGLVPYTGIMPIENTIDHVGPVSRTVRDN
ALLLEVIAGGDGLDPRQYHVKTEDYTSYLDRGVKGLKIGVVREGFNLANMDPKVAEKVRKAIVEFEKMGAIVEDISLPEH
ALAGALWSPVGCEGLTAQMMHGNGMGFNWEGLYGVKLIDKHKDWRKQADQLSPTLKACMFVGEYGIKNYSGRYYAKSKNI
TRRARAAYDQKLAEYDLLLMPTVPMVAQPLPEPGCSLTEYISKAFEMIGNTASQDITGHPAMSIPCGLVDDLPVGLMLIG
PYFGEGKIYQAAAAFEKNVDWKTI
>Mature_503_residues
AIQRPTIDVLKQIANDLHIQLTDTEAAEYLSIIQSNFDAYDLIDELDDEIPEVRYPRANSYRPDATENPLNAWYYKTEVK
GALSGKLSGKTIALKDNIFLADVPMMNGSTTLEGFVPEYDATIVTRMLDAGATILGKATCEHFCLSGGSHTSDPVAVHNP
YRHGYSAGGSSSGSAALVSNGDVDLAIACDQGGSIRIPAAFCGIYGMKPTHGLVPYTGIMPIENTIDHVGPVSRTVRDNA
LLLEVIAGGDGLDPRQYHVKTEDYTSYLDRGVKGLKIGVVREGFNLANMDPKVAEKVRKAIVEFEKMGAIVEDISLPEHA
LAGALWSPVGCEGLTAQMMHGNGMGFNWEGLYGVKLIDKHKDWRKQADQLSPTLKACMFVGEYGIKNYSGRYYAKSKNIT
RRARAAYDQKLAEYDLLLMPTVPMVAQPLPEPGCSLTEYISKAFEMIGNTASQDITGHPAMSIPCGLVDDLPVGLMLIGP
YFGEGKIYQAAAAFEKNVDWKTI

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=449, Percent_Identity=27.6169265033408, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI195972892, Length=517, Percent_Identity=24.3713733075435, Blast_Score=111, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17543272, Length=415, Percent_Identity=26.9879518072289, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17537465, Length=253, Percent_Identity=26.0869565217391, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=216, Percent_Identity=31.9444444444444, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319685, Length=424, Percent_Identity=25.4716981132075, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24648113, Length=445, Percent_Identity=30.3370786516854, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24644968, Length=424, Percent_Identity=25.7075471698113, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI161078093, Length=432, Percent_Identity=25.462962962963, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24652985, Length=373, Percent_Identity=25.4691689008043, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19922090, Length=373, Percent_Identity=25.4691689008043, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24652981, Length=373, Percent_Identity=25.4691689008043, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24652983, Length=373, Percent_Identity=25.4691689008043, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550774, Length=170, Percent_Identity=29.4117647058824, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648435, Length=170, Percent_Identity=29.4117647058824, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648437, Length=170, Percent_Identity=29.4117647058824, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356731, Length=242, Percent_Identity=30.9917355371901, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648441, Length=170, Percent_Identity=29.4117647058824, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648439, Length=170, Percent_Identity=29.4117647058824, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 54832; Mature: 54701

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIQRPTIDVLKQIANDLHIQLTDTEAAEYLSIIQSNFDAYDLIDELDDEIPEVRYPRAN
CCCCCCHHHHHHHHHCCEEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC
SYRPDATENPLNAWYYKTEVKGALSGKLSGKTIALKDNIFLADVPMMNGSTTLEGFVPEY
CCCCCCCCCCCCEEEEEEECCHHHCCCCCCCEEEEECCEEEEECCCCCCCCCEECCCCCC
DATIVTRMLDAGATILGKATCEHFCLSGGSHTSDPVAVHNPYRHGYSAGGSSSGSAALVS
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCHHCCCCCCCCCCCCEEEEE
NGDVDLAIACDQGGSIRIPAAFCGIYGMKPTHGLVPYTGIMPIENTIDHVGPVSRTVRDN
CCCEEEEEEECCCCCEECCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHCCC
ALLLEVIAGGDGLDPRQYHVKTEDYTSYLDRGVKGLKIGVVREGFNLANMDPKVAEKVRK
EEEEEEEECCCCCCCCEEEECCHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHH
AIVEFEKMGAIVEDISLPEHALAGALWSPVGCEGLTAQMMHGNGMGFNWEGLYGVKLIDK
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECC
HKDWRKQADQLSPTLKACMFVGEYGIKNYSGRYYAKSKNITRRARAAYDQKLAEYDLLLM
CHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCEEEE
PTVPMVAQPLPEPGCSLTEYISKAFEMIGNTASQDITGHPAMSIPCGLVDDLPVGLMLIG
CCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
PYFGEGKIYQAAAAFEKNVDWKTI
CCCCCCCEEHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AIQRPTIDVLKQIANDLHIQLTDTEAAEYLSIIQSNFDAYDLIDELDDEIPEVRYPRAN
CCCCCHHHHHHHHHCCEEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC
SYRPDATENPLNAWYYKTEVKGALSGKLSGKTIALKDNIFLADVPMMNGSTTLEGFVPEY
CCCCCCCCCCCCEEEEEEECCHHHCCCCCCCEEEEECCEEEEECCCCCCCCCEECCCCCC
DATIVTRMLDAGATILGKATCEHFCLSGGSHTSDPVAVHNPYRHGYSAGGSSSGSAALVS
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCHHCCCCCCCCCCCCEEEEE
NGDVDLAIACDQGGSIRIPAAFCGIYGMKPTHGLVPYTGIMPIENTIDHVGPVSRTVRDN
CCCEEEEEEECCCCCEECCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHCCC
ALLLEVIAGGDGLDPRQYHVKTEDYTSYLDRGVKGLKIGVVREGFNLANMDPKVAEKVRK
EEEEEEEECCCCCCCCEEEECCHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHH
AIVEFEKMGAIVEDISLPEHALAGALWSPVGCEGLTAQMMHGNGMGFNWEGLYGVKLIDK
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECC
HKDWRKQADQLSPTLKACMFVGEYGIKNYSGRYYAKSKNITRRARAAYDQKLAEYDLLLM
CHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCEEEE
PTVPMVAQPLPEPGCSLTEYISKAFEMIGNTASQDITGHPAMSIPCGLVDDLPVGLMLIG
CCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
PYFGEGKIYQAAAAFEKNVDWKTI
CCCCCCCEEHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2013568 [H]