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The map label for this gene is yjiA [C]

Identifier: 50084775

GI number: 50084775

Start: 1612689

End: 1613897

Strand: Reverse

Name: yjiA [C]

Synonym: ACIAD1614

Alternate gene names: 50084775

Gene position: 1613897-1612689 (Counterclockwise)

Preceding gene: 50084776

Following gene: 50084771

Centisome position: 44.85

GC content: 39.45

Gene sequence:

>1209_bases
ATGACTGACGTTATATTGAATCCCACTAAATATGTTAACTCCACACGCATTCCAGTGACTGTTTTATCAGGTTTTTTAGG
TTCTGGTAAAACAACTCTACTCAATTATATCTTGAACCATCAAAAAGATATGCGAATTGCTGTGATCGTGAATGATATGA
GTGAAATCAATATTGATGCAGAAGATGTACGTCGTCATGTTAATTTAAATAGAGGCAGTGACCAACTTATTGAAATGAGC
AATGGCTGTATTTGTTGCACATTAAGAGCTGATTTACTTGAACAAGTCAGCCTTCTCGCTCGCCAGGCCGAATTTGATTA
TATATTAATCGAATCTACAGGTATTTCAGAGCCTATGCCCGTTGCCGAAACCTTTGCATTTTTAGACCAACATGGATTTA
GTTTAAGCGAATTAGCGCGCCTGGATACCATGGTTACTGTCGTCAATGGTGAAAATTTTGAGCAAATGATTCAATCTCGT
GACAAGATCGAAATGGATCATCCTGATGAGGTTTCAGTTCAACGACATTTATCAGATTTGCTGTTGGAGCAGGTCGAATA
TGCAAATGTTATTTTAGTCAGTCGCATAGATATCATTGGTGAAGATCGATTCAAACATTTACGTTCTTTACTTGCAGAGC
TTAATCCATCCGCCAAGATTTTTCCAATTTCTCATGGTGCCATTGACCTCAACAACATTTTAAATACGTATACATTTGAT
TTACCCGATTTGGTTAAATCACCAGGATGGATGAAACATCTGGATCAGGAACAAGCGGTTCATTCTGAATCTGAAACTTA
TGGGATCTCGTCATGGGTATACAGTGCGCGCTTACCCTTTCATCCTCAACGGTTCCAGCACTTTTTAGCACACCCCTGGA
CTAACGGTACGCTACTCCGTTGTAAAGGTTATATATGGAATGCCAAGTGTTATCAGGACATTGGCATGCTTGTTCAAACA
GGTGGTCAGTTTGAATGGGAAATCGTTGGGCGCTGGTGGAACTTTATTAATCAATCAGACTGGCCTCAAGACCATTATCG
ACGCGATAGTATTTTTGAAAAATGGGATGAGCGTACAGGGGACTGTCGTCAGGAAATTGTATTTATTGGCCAAAATATTG
ACTGGAACACACTCAAACATCAACTTGATGATTGTCTGTTAACACTTCAGGAAATTGAAGATGGCCCCGAAGCATGGCTT
AATTATTAA

Upstream 100 bases:

>100_bases
GATGCTGGATATGTAGTAGTCGATTTATTTGAAAGTTATCTCGACAAAGCCACAGCATAGTTGATCAATACAAAATCACT
GCCTTTAGGAGAATAAAAAC

Downstream 100 bases:

>100_bases
TTTTTATCTACCCGCTTAAACATTCATGCACATTTCACAAACTACAATGTGCATGAATAATACAAAGCAATATAATTACA
AACTTCTAGAAATGACTATA

Product: nitrile hydratase activator

Products: NA

Alternate protein names: P47K [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTDVILNPTKYVNSTRIPVTVLSGFLGSGKTTLLNYILNHQKDMRIAVIVNDMSEINIDAEDVRRHVNLNRGSDQLIEMS
NGCICCTLRADLLEQVSLLARQAEFDYILIESTGISEPMPVAETFAFLDQHGFSLSELARLDTMVTVVNGENFEQMIQSR
DKIEMDHPDEVSVQRHLSDLLLEQVEYANVILVSRIDIIGEDRFKHLRSLLAELNPSAKIFPISHGAIDLNNILNTYTFD
LPDLVKSPGWMKHLDQEQAVHSESETYGISSWVYSARLPFHPQRFQHFLAHPWTNGTLLRCKGYIWNAKCYQDIGMLVQT
GGQFEWEIVGRWWNFINQSDWPQDHYRRDSIFEKWDERTGDCRQEIVFIGQNIDWNTLKHQLDDCLLTLQEIEDGPEAWL
NY

Sequences:

>Translated_402_residues
MTDVILNPTKYVNSTRIPVTVLSGFLGSGKTTLLNYILNHQKDMRIAVIVNDMSEINIDAEDVRRHVNLNRGSDQLIEMS
NGCICCTLRADLLEQVSLLARQAEFDYILIESTGISEPMPVAETFAFLDQHGFSLSELARLDTMVTVVNGENFEQMIQSR
DKIEMDHPDEVSVQRHLSDLLLEQVEYANVILVSRIDIIGEDRFKHLRSLLAELNPSAKIFPISHGAIDLNNILNTYTFD
LPDLVKSPGWMKHLDQEQAVHSESETYGISSWVYSARLPFHPQRFQHFLAHPWTNGTLLRCKGYIWNAKCYQDIGMLVQT
GGQFEWEIVGRWWNFINQSDWPQDHYRRDSIFEKWDERTGDCRQEIVFIGQNIDWNTLKHQLDDCLLTLQEIEDGPEAWL
NY
>Mature_401_residues
TDVILNPTKYVNSTRIPVTVLSGFLGSGKTTLLNYILNHQKDMRIAVIVNDMSEINIDAEDVRRHVNLNRGSDQLIEMSN
GCICCTLRADLLEQVSLLARQAEFDYILIESTGISEPMPVAETFAFLDQHGFSLSELARLDTMVTVVNGENFEQMIQSRD
KIEMDHPDEVSVQRHLSDLLLEQVEYANVILVSRIDIIGEDRFKHLRSLLAELNPSAKIFPISHGAIDLNNILNTYTFDL
PDLVKSPGWMKHLDQEQAVHSESETYGISSWVYSARLPFHPQRFQHFLAHPWTNGTLLRCKGYIWNAKCYQDIGMLVQTG
GQFEWEIVGRWWNFINQSDWPQDHYRRDSIFEKWDERTGDCRQEIVFIGQNIDWNTLKHQLDDCLLTLQEIEDGPEAWLN
Y

Specific function: The presence of P47K is critical for the expression of the nitrile hydratase genes. May stabilize or activate the nitrile hydratase proteins [H]

COG id: COG0523

COG function: function code R; Putative GTPases (G3E family)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cobW C-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI33469141, Length=255, Percent_Identity=30.5882352941176, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI126722884, Length=255, Percent_Identity=30.5882352941176, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI146231952, Length=255, Percent_Identity=30.5882352941176, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI148727351, Length=255, Percent_Identity=30.1960784313725, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI223941779, Length=248, Percent_Identity=29.0322580645161, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI223941776, Length=242, Percent_Identity=29.7520661157025, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI119120938, Length=142, Percent_Identity=33.8028169014084, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI87082430, Length=300, Percent_Identity=30.6666666666667, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324356, Length=235, Percent_Identity=28.5106382978723, Blast_Score=107, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR011629 [H]

Pfam domain/function: PF02492 cobW; PF07683 CobW_C [H]

EC number: NA

Molecular weight: Translated: 46418; Mature: 46287

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVILNPTKYVNSTRIPVTVLSGFLGSGKTTLLNYILNHQKDMRIAVIVNDMSEINIDA
CCCEEECCHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEECHHHCCCCH
EDVRRHVNLNRGSDQLIEMSNGCICCTLRADLLEQVSLLARQAEFDYILIESTGISEPMP
HHHHHHCCCCCCCHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
VAETFAFLDQHGFSLSELARLDTMVTVVNGENFEQMIQSRDKIEMDHPDEVSVQRHLSDL
HHHHHHHHHHCCCCHHHHHHHHHEEEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LLEQVEYANVILVSRIDIIGEDRFKHLRSLLAELNPSAKIFPISHGAIDLNNILNTYTFD
HHHHHHHHCEEEEEHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHHHHHCCC
LPDLVKSPGWMKHLDQEQAVHSESETYGISSWVYSARLPFHPQRFQHFLAHPWTNGTLLR
CHHHHHCCCHHHHCCHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEE
CKGYIWNAKCYQDIGMLVQTGGQFEWEIVGRWWNFINQSDWPQDHYRRDSIFEKWDERTG
EECEEECCHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
DCRQEIVFIGQNIDWNTLKHQLDDCLLTLQEIEDGPEAWLNY
HHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure 
TDVILNPTKYVNSTRIPVTVLSGFLGSGKTTLLNYILNHQKDMRIAVIVNDMSEINIDA
CCEEECCHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEECHHHCCCCH
EDVRRHVNLNRGSDQLIEMSNGCICCTLRADLLEQVSLLARQAEFDYILIESTGISEPMP
HHHHHHCCCCCCCHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
VAETFAFLDQHGFSLSELARLDTMVTVVNGENFEQMIQSRDKIEMDHPDEVSVQRHLSDL
HHHHHHHHHHCCCCHHHHHHHHHEEEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LLEQVEYANVILVSRIDIIGEDRFKHLRSLLAELNPSAKIFPISHGAIDLNNILNTYTFD
HHHHHHHHCEEEEEHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHHHHHCCC
LPDLVKSPGWMKHLDQEQAVHSESETYGISSWVYSARLPFHPQRFQHFLAHPWTNGTLLR
CHHHHHCCCHHHHCCHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEE
CKGYIWNAKCYQDIGMLVQTGGQFEWEIVGRWWNFINQSDWPQDHYRRDSIFEKWDERTG
EECEEECCHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
DCRQEIVFIGQNIDWNTLKHQLDDCLLTLQEIEDGPEAWLNY
HHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2013568 [H]