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The map label for this gene is paaF [C]

Identifier: 50084770

GI number: 50084770

Start: 1606007

End: 1607035

Strand: Direct

Name: paaF [C]

Synonym: ACIAD1609

Alternate gene names: 50084770

Gene position: 1606007-1607035 (Clockwise)

Preceding gene: 50084769

Following gene: 50084772

Centisome position: 44.63

GC content: 36.15

Gene sequence:

>1029_bases
ATGACTACTACTGACAATCATTTACTCATTGAACATAAAAACGCTTTAGGAACAATTATTTTAAATCGTCCAGCGAGTCT
GAACGCGCTATCTCTAGAAATGATTAATGCGATTCGTCAACAAGTTGAGGATTGGCAAGGTGATGTAAATGTTCAGGCCA
TATTAATTAAATCAAATAGTCCTAAAGCATTTTGTGCAGGTGGTGATATTCGCTATCTTTATGAAAGTTATAAAAGTGGA
TCAGAAGAGTATAAAGATTATTTCATTGCTGAATATGAGATGCTCAATAGCATTCGAACGTCTAAAAAAACAGTGATTGT
TTTATTGGATGGATATGTATTGGGTGGTGGTTTTGGTTTAGCACAGGCTTGTCATATCTTGGTGAGTAGTGAAAAATCAC
GATTTTCAATGCCAGAAACAGCAATAGGTTTTTTCCCAGATGTTGCAGCGACTTATTTCTTATCTCGTTTAGATGATGTT
GGGGTATATTTGGCACTGACTGGTGATCAAATCAGTAGTAGTGATGCATTGTATTTAGATCTGATTGATTATCATGTTCC
GAGTCAGAATTTTGAGCGACTAGAAAATGCATTCAGCCAATCACAGAACTTAGATAAATTTCATATTCAGAAGATTATTT
CTGCTTATATCTCCAGCCCTGTTCAGAGTGAACTCAGTCTATGGCTTGAAGCCATTCGTCAGCATTTTGGTCTTAAAAAT
GTGCAAGATATCGAAGAAAGTTTGAAAAATGAACAAGATCCCAACTATCAAGTATGGACAAGTAAAGTGTTAAATACTTT
GCAACAACGTTCCTCTATTGCAAAAAAAACCAGTTTAAAGTTACAGCTGCTAGGGCGTGGATGGTCATTACAGCAATGTA
TGCGTATCGAGCGAAAATTACAGGATATCTGGTTTGAACATGGTGATATGATTGAGGGTGTTCGAGCGTTGATTATTGAT
AAAGATAAACAACCGCAATGGCAGCAGCATAATGCGACTTTAGATAATATATTAGGCCAATTAGGTTAG

Upstream 100 bases:

>100_bases
GTTCCATTCAGTTTTTGCGACCTTAGATCAGAAAGAAGGCATGCAAGCATTTATTGATAAACGTCCAGCCCAATTTAAAC
ATCAATAATAGGATGAAGCG

Downstream 100 bases:

>100_bases
TCATTTCAGACTGACTTACTGGCAAGCGTAAGAGATTTCTTGCTGCTATTCCGCTAGTTCTAAATATGCTTTTATGCAGA
GACAGGCGGAATTTTGTGCT

Product: enoyl-CoA hydratase/isomerase family protein

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MTTTDNHLLIEHKNALGTIILNRPASLNALSLEMINAIRQQVEDWQGDVNVQAILIKSNSPKAFCAGGDIRYLYESYKSG
SEEYKDYFIAEYEMLNSIRTSKKTVIVLLDGYVLGGGFGLAQACHILVSSEKSRFSMPETAIGFFPDVAATYFLSRLDDV
GVYLALTGDQISSSDALYLDLIDYHVPSQNFERLENAFSQSQNLDKFHIQKIISAYISSPVQSELSLWLEAIRQHFGLKN
VQDIEESLKNEQDPNYQVWTSKVLNTLQQRSSIAKKTSLKLQLLGRGWSLQQCMRIERKLQDIWFEHGDMIEGVRALIID
KDKQPQWQQHNATLDNILGQLG

Sequences:

>Translated_342_residues
MTTTDNHLLIEHKNALGTIILNRPASLNALSLEMINAIRQQVEDWQGDVNVQAILIKSNSPKAFCAGGDIRYLYESYKSG
SEEYKDYFIAEYEMLNSIRTSKKTVIVLLDGYVLGGGFGLAQACHILVSSEKSRFSMPETAIGFFPDVAATYFLSRLDDV
GVYLALTGDQISSSDALYLDLIDYHVPSQNFERLENAFSQSQNLDKFHIQKIISAYISSPVQSELSLWLEAIRQHFGLKN
VQDIEESLKNEQDPNYQVWTSKVLNTLQQRSSIAKKTSLKLQLLGRGWSLQQCMRIERKLQDIWFEHGDMIEGVRALIID
KDKQPQWQQHNATLDNILGQLG
>Mature_341_residues
TTTDNHLLIEHKNALGTIILNRPASLNALSLEMINAIRQQVEDWQGDVNVQAILIKSNSPKAFCAGGDIRYLYESYKSGS
EEYKDYFIAEYEMLNSIRTSKKTVIVLLDGYVLGGGFGLAQACHILVSSEKSRFSMPETAIGFFPDVAATYFLSRLDDVG
VYLALTGDQISSSDALYLDLIDYHVPSQNFERLENAFSQSQNLDKFHIQKIISAYISSPVQSELSLWLEAIRQHFGLKNV
QDIEESLKNEQDPNYQVWTSKVLNTLQQRSSIAKKTSLKLQLLGRGWSLQQCMRIERKLQDIWFEHGDMIEGVRALIIDK
DKQPQWQQHNATLDNILGQLG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=333, Percent_Identity=34.5345345345345, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI37594469, Length=305, Percent_Identity=33.7704918032787, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI194097323, Length=218, Percent_Identity=31.651376146789, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI68989263, Length=297, Percent_Identity=26.5993265993266, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI31542718, Length=177, Percent_Identity=27.683615819209, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1787659, Length=188, Percent_Identity=27.6595744680851, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788597, Length=186, Percent_Identity=26.3440860215054, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788682, Length=177, Percent_Identity=28.2485875706215, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI221142681, Length=188, Percent_Identity=26.5957446808511, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144157, Length=339, Percent_Identity=33.3333333333333, Blast_Score=191, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI25144160, Length=339, Percent_Identity=33.3333333333333, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17554946, Length=181, Percent_Identity=29.2817679558011, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=27.1255060728745, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540714, Length=202, Percent_Identity=27.2277227722772, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=160, Percent_Identity=28.125, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=143, Percent_Identity=30.0699300699301, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=162, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=172, Percent_Identity=29.6511627906977, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=143, Percent_Identity=30.0699300699301, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=187, Percent_Identity=34.7593582887701, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI28571729, Length=314, Percent_Identity=37.5796178343949, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI28571730, Length=314, Percent_Identity=37.5796178343949, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI20129971, Length=181, Percent_Identity=32.0441988950276, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24653477, Length=181, Percent_Identity=32.0441988950276, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24653139, Length=206, Percent_Identity=28.6407766990291, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922422, Length=189, Percent_Identity=23.2804232804233, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921000, Length=188, Percent_Identity=28.7234042553192, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24583077, Length=188, Percent_Identity=28.7234042553192, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24583079, Length=188, Percent_Identity=28.7234042553192, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 38771; Mature: 38640

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTDNHLLIEHKNALGTIILNRPASLNALSLEMINAIRQQVEDWQGDVNVQAILIKSNS
CCCCCCEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
PKAFCAGGDIRYLYESYKSGSEEYKDYFIAEYEMLNSIRTSKKTVIVLLDGYVLGGGFGL
CCEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECEEECCCHHH
AQACHILVSSEKSRFSMPETAIGFFPDVAATYFLSRLDDVGVYLALTGDQISSSDALYLD
HHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEE
LIDYHVPSQNFERLENAFSQSQNLDKFHIQKIISAYISSPVQSELSLWLEAIRQHFGLKN
EHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
VQDIEESLKNEQDPNYQVWTSKVLNTLQQRSSIAKKTSLKLQLLGRGWSLQQCMRIERKL
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHH
QDIWFEHGDMIEGVRALIIDKDKQPQWQQHNATLDNILGQLG
HHHHHHCCCHHHHHHHHEECCCCCCCHHHHCCHHHHHHHCCC
>Mature Secondary Structure 
TTTDNHLLIEHKNALGTIILNRPASLNALSLEMINAIRQQVEDWQGDVNVQAILIKSNS
CCCCCEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
PKAFCAGGDIRYLYESYKSGSEEYKDYFIAEYEMLNSIRTSKKTVIVLLDGYVLGGGFGL
CCEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECEEECCCHHH
AQACHILVSSEKSRFSMPETAIGFFPDVAATYFLSRLDDVGVYLALTGDQISSSDALYLD
HHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEE
LIDYHVPSQNFERLENAFSQSQNLDKFHIQKIISAYISSPVQSELSLWLEAIRQHFGLKN
EHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
VQDIEESLKNEQDPNYQVWTSKVLNTLQQRSSIAKKTSLKLQLLGRGWSLQQCMRIERKL
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHH
QDIWFEHGDMIEGVRALIIDKDKQPQWQQHNATLDNILGQLG
HHHHHHCCCHHHHHHHHEECCCCCCCHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA