Click here to switch to the map view.

The map label for this gene is lcfB [H]

Identifier: 50084734

GI number: 50084734

Start: 1563341

End: 1564894

Strand: Direct

Name: lcfB [H]

Synonym: ACIAD1572

Alternate gene names: 50084734

Gene position: 1563341-1564894 (Clockwise)

Preceding gene: 50084733

Following gene: 50084736

Centisome position: 43.44

GC content: 43.37

Gene sequence:

>1554_bases
ATGCATCCAATGGTTCATGCACAGAACACCCCTGATAAAGCGGCTTGTATCTTTGCCAGTACGCAGCAAGTTCTAAGCTA
TGCACAAATGAATGCGTTGGCAAATCGCTGCGCACATTTATTTCGTCAACATGGTCTCAAGCGTGGTGATGTGGTTTCGA
TATTGCTTGAAAACAGTATCGATATTTTTACGGTGGCGTGGGCAGCACAGCGTTCAGGACTTTATCTCACCGCGATTTCG
TGTAAAACCTCCGCAAAAGATCTGGCTTATATTCTAGATAATTCAGAAAGCAAAATATTGATTGTCTCGGAATGTTTGGT
CGATACCGCTTTAGAAGCACTCCAATTGAGTCAACTGGATTATGTCTATTTATATGCCACAGGGCAAAAAACCTCAGTGG
CTTCTTTCACACAAGCGCTCAGCCAATGTCGGGAAGATGATATTACAGACCCAAGTCCAGGCGGGGATATGTTGTATTCT
TCTGGTTCTACGGGTCGTCCTAAAGGTGTACGTCCGCCTTTACCTGAAGGTGATTTGATGCAGCCAGTGCCACTAATGGC
GATGGGACGTGATTTATACCAGATGAATCAAGACACCCTGTATTTATCGACCTCACCGCTTTATCATGCTGCGCCGTTAC
GATGGGCCTTAGCTGTTCATCGTTTTGGTGGCACTGTGGTGATCATGGATAAGTATGATGCAGAGCAAACGCTCAATTTA
ATTGAGCAATATCAGATCAGTCATGCCACCTTTGTGCCGACACATTTCATTCGGTTACTTAACTTGCCAGAAACCAGACG
TCAGAGTTTTGATTCATCCTCGCTTAAGGCTGTCATTCATGCGGCTGCACCGTGTCCCGTACCAGTTAAGCAGGCAATGA
TTGACTGGTGGGGACCACTTTTACATGAATATTATTCAGGCACTGAACAATGTGGGATTACTGCATTGGATTCAGTGCAA
TGGCTTGATAAACCAGGTTCAGTCGGTAAAGCAGTACTAGGAAAAATTAAAGTACTGGATGAACAGCAAAACGAACTGGC
TGTGGGGCAAATTGGTGATATTTATTTTGCGGATGGCCCACAGTTTGAATACTACAAAGACCCTGAAAAAACTAAAACAG
CTTATAGCAAGCAAGGTTGGGCCACATTGGGCGATATTGGCTGGGTAGATGAAGATGGCTATTTATATCTGACCGATCGT
AAAAACTTCATGATTATTTCGGGTGGAGTCAATATTTATCCTCAAGAAATAGAAAATCTATTAATGACACATCCTGATGT
GAATGATACCGCCGTTTTTGGAATTCCTGATGACGAAATGGGCGAAAAAGTGGTTGCAATTGTTCAGCTTAAAGCGTGTA
TTTTGTCGAGCGCAGATGAAGCCCAGAAACTCAAACAATTTGTGCGTCAAGCTTTAGGTGGTGTGAAATGCCCACAGGTT
TTTGAATTTTGTCAAAACTTTCCAAGAGAAGCGACAGGGAAAATACTGAAACGCAAACTGATTGAAGATTATAAAGCGCA
TTTGGCTTCTTCAACCCAAGCTCACTCGGCATGA

Upstream 100 bases:

>100_bases
GTGATTGAAGGACCAAAAGCATTTGCACAGAAACGCACACCAGAATGGAAAGGGCGTTAATTTTCAAATTAAAAATGCCA
CATGGAATGAGGATCAAGAG

Downstream 100 bases:

>100_bases
TACAGGAGATAGCCACGCAAGACGACATCGTGATTTTAAGAGCAGTTTATAAGTTCGATGATTATTGAAATCATATCAAG
ATGTCTATGAATACATTATT

Product: long-chain-fatty-acid--CoA ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MHPMVHAQNTPDKAACIFASTQQVLSYAQMNALANRCAHLFRQHGLKRGDVVSILLENSIDIFTVAWAAQRSGLYLTAIS
CKTSAKDLAYILDNSESKILIVSECLVDTALEALQLSQLDYVYLYATGQKTSVASFTQALSQCREDDITDPSPGGDMLYS
SGSTGRPKGVRPPLPEGDLMQPVPLMAMGRDLYQMNQDTLYLSTSPLYHAAPLRWALAVHRFGGTVVIMDKYDAEQTLNL
IEQYQISHATFVPTHFIRLLNLPETRRQSFDSSSLKAVIHAAAPCPVPVKQAMIDWWGPLLHEYYSGTEQCGITALDSVQ
WLDKPGSVGKAVLGKIKVLDEQQNELAVGQIGDIYFADGPQFEYYKDPEKTKTAYSKQGWATLGDIGWVDEDGYLYLTDR
KNFMIISGGVNIYPQEIENLLMTHPDVNDTAVFGIPDDEMGEKVVAIVQLKACILSSADEAQKLKQFVRQALGGVKCPQV
FEFCQNFPREATGKILKRKLIEDYKAHLASSTQAHSA

Sequences:

>Translated_517_residues
MHPMVHAQNTPDKAACIFASTQQVLSYAQMNALANRCAHLFRQHGLKRGDVVSILLENSIDIFTVAWAAQRSGLYLTAIS
CKTSAKDLAYILDNSESKILIVSECLVDTALEALQLSQLDYVYLYATGQKTSVASFTQALSQCREDDITDPSPGGDMLYS
SGSTGRPKGVRPPLPEGDLMQPVPLMAMGRDLYQMNQDTLYLSTSPLYHAAPLRWALAVHRFGGTVVIMDKYDAEQTLNL
IEQYQISHATFVPTHFIRLLNLPETRRQSFDSSSLKAVIHAAAPCPVPVKQAMIDWWGPLLHEYYSGTEQCGITALDSVQ
WLDKPGSVGKAVLGKIKVLDEQQNELAVGQIGDIYFADGPQFEYYKDPEKTKTAYSKQGWATLGDIGWVDEDGYLYLTDR
KNFMIISGGVNIYPQEIENLLMTHPDVNDTAVFGIPDDEMGEKVVAIVQLKACILSSADEAQKLKQFVRQALGGVKCPQV
FEFCQNFPREATGKILKRKLIEDYKAHLASSTQAHSA
>Mature_517_residues
MHPMVHAQNTPDKAACIFASTQQVLSYAQMNALANRCAHLFRQHGLKRGDVVSILLENSIDIFTVAWAAQRSGLYLTAIS
CKTSAKDLAYILDNSESKILIVSECLVDTALEALQLSQLDYVYLYATGQKTSVASFTQALSQCREDDITDPSPGGDMLYS
SGSTGRPKGVRPPLPEGDLMQPVPLMAMGRDLYQMNQDTLYLSTSPLYHAAPLRWALAVHRFGGTVVIMDKYDAEQTLNL
IEQYQISHATFVPTHFIRLLNLPETRRQSFDSSSLKAVIHAAAPCPVPVKQAMIDWWGPLLHEYYSGTEQCGITALDSVQ
WLDKPGSVGKAVLGKIKVLDEQQNELAVGQIGDIYFADGPQFEYYKDPEKTKTAYSKQGWATLGDIGWVDEDGYLYLTDR
KNFMIISGGVNIYPQEIENLLMTHPDVNDTAVFGIPDDEMGEKVVAIVQLKACILSSADEAQKLKQFVRQALGGVKCPQV
FEFCQNFPREATGKILKRKLIEDYKAHLASSTQAHSA

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=549, Percent_Identity=24.408014571949, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI187761345, Length=498, Percent_Identity=25.5020080321285, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI187761343, Length=498, Percent_Identity=25.5020080321285, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI122937307, Length=499, Percent_Identity=27.6553106212425, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI115511026, Length=500, Percent_Identity=26.4, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI157311624, Length=492, Percent_Identity=27.0325203252033, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI157311622, Length=492, Percent_Identity=27.0325203252033, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI58082049, Length=492, Percent_Identity=27.4390243902439, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI38505220, Length=502, Percent_Identity=26.8924302788845, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI42544132, Length=496, Percent_Identity=24.5967741935484, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI13375727, Length=549, Percent_Identity=22.7686703096539, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI8923896, Length=587, Percent_Identity=21.8057921635434, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI227499619, Length=546, Percent_Identity=21.2454212454212, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI253970489, Length=382, Percent_Identity=23.8219895287958, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788107, Length=538, Percent_Identity=25.8364312267658, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI221142682, Length=517, Percent_Identity=27.0793036750484, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI145693145, Length=524, Percent_Identity=25.9541984732824, Blast_Score=122, Evalue=8e-29,
Organism=Escherichia coli, GI1786810, Length=502, Percent_Identity=25.4980079681275, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790505, Length=541, Percent_Identity=23.8447319778189, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1786529, Length=545, Percent_Identity=22.3853211009174, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17531443, Length=521, Percent_Identity=26.679462571977, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32563687, Length=507, Percent_Identity=26.0355029585799, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17558820, Length=364, Percent_Identity=29.6703296703297, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI71994703, Length=543, Percent_Identity=23.7569060773481, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71994694, Length=543, Percent_Identity=23.7569060773481, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71994690, Length=545, Percent_Identity=24.0366972477064, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17557194, Length=540, Percent_Identity=26.1111111111111, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=536, Percent_Identity=25.3731343283582, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=538, Percent_Identity=23.9776951672862, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17559526, Length=376, Percent_Identity=30.0531914893617, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17560140, Length=481, Percent_Identity=24.1164241164241, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI115534440, Length=549, Percent_Identity=22.7686703096539, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17551278, Length=555, Percent_Identity=24.3243243243243, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=242, Percent_Identity=30.9917355371901, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=354, Percent_Identity=26.271186440678, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319264, Length=561, Percent_Identity=22.9946524064171, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355181, Length=495, Percent_Identity=27.6767676767677, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19922652, Length=520, Percent_Identity=26.1538461538462, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI281365686, Length=473, Percent_Identity=25.7928118393235, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI21356441, Length=460, Percent_Identity=25.8695652173913, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24648260, Length=513, Percent_Identity=25.3411306042885, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21356947, Length=509, Percent_Identity=24.5579567779961, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24581924, Length=546, Percent_Identity=23.8095238095238, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24648253, Length=468, Percent_Identity=24.1452991452991, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24648255, Length=468, Percent_Identity=23.9316239316239, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24656500, Length=521, Percent_Identity=24.1842610364683, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21358303, Length=459, Percent_Identity=25.9259259259259, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648257, Length=538, Percent_Identity=24.7211895910781, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI20130357, Length=511, Percent_Identity=26.027397260274, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI18859661, Length=526, Percent_Identity=24.7148288973384, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24653035, Length=519, Percent_Identity=23.6994219653179, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI161076582, Length=538, Percent_Identity=23.6059479553903, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI62472339, Length=544, Percent_Identity=23.7132352941176, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24667955, Length=544, Percent_Identity=23.7132352941176, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21355225, Length=531, Percent_Identity=24.105461393597, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24667959, Length=467, Percent_Identity=24.4111349036403, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281366413, Length=340, Percent_Identity=25.5882352941176, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24666501, Length=338, Percent_Identity=25.7396449704142, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24666497, Length=338, Percent_Identity=25.7396449704142, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 57245; Mature: 57245

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPMVHAQNTPDKAACIFASTQQVLSYAQMNALANRCAHLFRQHGLKRGDVVSILLENSI
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
DIFTVAWAAQRSGLYLTAISCKTSAKDLAYILDNSESKILIVSECLVDTALEALQLSQLD
CEEEEEEECCCCCEEEEEEECCCCCHHEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCC
YVYLYATGQKTSVASFTQALSQCREDDITDPSPGGDMLYSSGSTGRPKGVRPPLPEGDLM
EEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCC
QPVPLMAMGRDLYQMNQDTLYLSTSPLYHAAPLRWALAVHRFGGTVVIMDKYDAEQTLNL
CCCCHHHHCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
IEQYQISHATFVPTHFIRLLNLPETRRQSFDSSSLKAVIHAAAPCPVPVKQAMIDWWGPL
HHHHHHCCEEEHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LHEYYSGTEQCGITALDSVQWLDKPGSVGKAVLGKIKVLDEQQNELAVGQIGDIYFADGP
HHHHHCCCHHCCCEECCCHHHHCCCCCHHHHHHHHHHEECCCCCCEEEECCCCEEECCCC
QFEYYKDPEKTKTAYSKQGWATLGDIGWVDEDGYLYLTDRKNFMIISGGVNIYPQEIENL
CCCCCCCCHHHHHHHHCCCCEEECCCCEECCCCEEEEECCCCEEEEECCCEECHHHHHHH
LMTHPDVNDTAVFGIPDDEMGEKVVAIVQLKACILSSADEAQKLKQFVRQALGGVKCPQV
HHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHH
FEFCQNFPREATGKILKRKLIEDYKAHLASSTQAHSA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MHPMVHAQNTPDKAACIFASTQQVLSYAQMNALANRCAHLFRQHGLKRGDVVSILLENSI
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
DIFTVAWAAQRSGLYLTAISCKTSAKDLAYILDNSESKILIVSECLVDTALEALQLSQLD
CEEEEEEECCCCCEEEEEEECCCCCHHEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCC
YVYLYATGQKTSVASFTQALSQCREDDITDPSPGGDMLYSSGSTGRPKGVRPPLPEGDLM
EEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCC
QPVPLMAMGRDLYQMNQDTLYLSTSPLYHAAPLRWALAVHRFGGTVVIMDKYDAEQTLNL
CCCCHHHHCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
IEQYQISHATFVPTHFIRLLNLPETRRQSFDSSSLKAVIHAAAPCPVPVKQAMIDWWGPL
HHHHHHCCEEEHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LHEYYSGTEQCGITALDSVQWLDKPGSVGKAVLGKIKVLDEQQNELAVGQIGDIYFADGP
HHHHHCCCHHCCCEECCCHHHHCCCCCHHHHHHHHHHEECCCCCCEEEECCCCEEECCCC
QFEYYKDPEKTKTAYSKQGWATLGDIGWVDEDGYLYLTDRKNFMIISGGVNIYPQEIENL
CCCCCCCCHHHHHHHHCCCCEEECCCCEECCCCEEEEECCCCEEEEECCCEECHHHHHHH
LMTHPDVNDTAVFGIPDDEMGEKVVAIVQLKACILSSADEAQKLKQFVRQALGGVKCPQV
HHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHH
FEFCQNFPREATGKILKRKLIEDYKAHLASSTQAHSA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]