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The map label for this gene is citA [H]

Identifier: 50084706

GI number: 50084706

Start: 1542461

End: 1543771

Strand: Reverse

Name: citA [H]

Synonym: ACIAD1541

Alternate gene names: 50084706

Gene position: 1543771-1542461 (Counterclockwise)

Preceding gene: 50084708

Following gene: 50084705

Centisome position: 42.9

GC content: 43.55

Gene sequence:

>1311_bases
ATGGAACAGGTTGCTAAACGTAGTGCTACGTTTAAAAAAATTCTAAATGTCACCAGTGGCAATTTCTTAGAACAATATGA
TTTTTTTCTTTTTGGATTTTATGCAACCTATATTGCAGCCAAGTTTTTTCATTCAGAAAATGAATACGTCTCACTTATGA
TGACCTTTACCGTATTCGCGGCAGGTTTTTTAATGCGACCGTTGGGTGCGATTTTTCTAGGCGCCTATGTCGACCGCGTG
GGACGTCGTAAAGGTCTGATTATCACTTTAAGTTTAATGGCACTGGGTACTATTTTAATTACCTTTGTTCCAAGTTACGA
TGCGATCGGCATCATTGCACCCATCTTGGTGGTGATCGGACGATTATTGCAAGGATTTTCTGCGGGTGTTGAATCCGGTG
GTGTATCGATTTATCTAGCCGAAATTGCCACAGACAAAAACCGTGGTTTTATTACTAGTTGGCAATCGGGTAGCCAGCAA
ATTGCAGTAGTATTTGCAGCGTTACTGGGTTATTGGCTGAATTCCGTATTGAGCCATGCTCAGGTCAGTGACTGGGGCTG
GCGTATTCCATTTTTAATTGGATGTTTGATCATTCCGCTTATTTTTGTGTTCCGTCGTACGCTGGAAGAAACAGAAGACT
TTAAAGCACAAAAAACCCATCCAACGACCAAAGAAATTTTTAGCACGCTTGCAAGTAACTGGCGCATTGTACTGGCCGGT
ATGATGATGAGTGCCATGACCACGACCACGTTCTATTTCATTACGGTTTACACCACTGTCTATGCAAAACGTACTCTAGA
AATGTCGATCACAGATAGTCTTTTAGCAACTGTATTTGTAGGGTTATCCAATTTCTTCTGGTTACCTATGGGTGGCTTGC
TTTCCGACAAAATTGGCCGTCGTCCTGTGCTGGTCGGCATTACGACACTTGCAATTTTTACCAGTTATCCGGTTTTAAGC
TGGTTGGTCAGTGACATTAGTTTTACCAACCTAATCATTGCACTTGCGTACTTCTCTTTCTTCTTTGGTCTGTATAACGG
CACCATGGTTGCCACGCTGGCAGAAGTAATGCCAAAACGCGTACGTACAGTTGGCTTTTCTTTGGCATTTAGTCTTGCCG
CTGCGATTTTTGGTGGCATGACGCCGATGGCGTGCACCTTCTTGGTTGAGCACACTGGCAACCCAAGCACTCCGGCTTTT
TGGCTCATGTTTGCTGCCGTATGTAGCCTGATCTCAAGCCTGTATTTATGCCGTGGTGCTAAAAAGAACAATCCTGACGC
AGTTGTTGAACCAGCAAAACTGAGTGTCTAA

Upstream 100 bases:

>100_bases
GACGTTGTGTCACGATCAATACGACACCTTAAGACGGACCATGCGGTCATAAAAAAAGAGACACGTGATGGTTTCCATTT
TTCAGACCAAGGAGGTCTTA

Downstream 100 bases:

>100_bases
CACGTCAGCATCATTTGAAAATTTTAATGAACAGCACGGCGTGCATATTGCAACCGTGCTGTGTTCTTGTGTCCTTCAAG
TGAGTTACTATGAAAAAATC

Product: major facilitator superfamily citrate-proton symporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MEQVAKRSATFKKILNVTSGNFLEQYDFFLFGFYATYIAAKFFHSENEYVSLMMTFTVFAAGFLMRPLGAIFLGAYVDRV
GRRKGLIITLSLMALGTILITFVPSYDAIGIIAPILVVIGRLLQGFSAGVESGGVSIYLAEIATDKNRGFITSWQSGSQQ
IAVVFAALLGYWLNSVLSHAQVSDWGWRIPFLIGCLIIPLIFVFRRTLEETEDFKAQKTHPTTKEIFSTLASNWRIVLAG
MMMSAMTTTTFYFITVYTTVYAKRTLEMSITDSLLATVFVGLSNFFWLPMGGLLSDKIGRRPVLVGITTLAIFTSYPVLS
WLVSDISFTNLIIALAYFSFFFGLYNGTMVATLAEVMPKRVRTVGFSLAFSLAAAIFGGMTPMACTFLVEHTGNPSTPAF
WLMFAAVCSLISSLYLCRGAKKNNPDAVVEPAKLSV

Sequences:

>Translated_436_residues
MEQVAKRSATFKKILNVTSGNFLEQYDFFLFGFYATYIAAKFFHSENEYVSLMMTFTVFAAGFLMRPLGAIFLGAYVDRV
GRRKGLIITLSLMALGTILITFVPSYDAIGIIAPILVVIGRLLQGFSAGVESGGVSIYLAEIATDKNRGFITSWQSGSQQ
IAVVFAALLGYWLNSVLSHAQVSDWGWRIPFLIGCLIIPLIFVFRRTLEETEDFKAQKTHPTTKEIFSTLASNWRIVLAG
MMMSAMTTTTFYFITVYTTVYAKRTLEMSITDSLLATVFVGLSNFFWLPMGGLLSDKIGRRPVLVGITTLAIFTSYPVLS
WLVSDISFTNLIIALAYFSFFFGLYNGTMVATLAEVMPKRVRTVGFSLAFSLAAAIFGGMTPMACTFLVEHTGNPSTPAF
WLMFAAVCSLISSLYLCRGAKKNNPDAVVEPAKLSV
>Mature_436_residues
MEQVAKRSATFKKILNVTSGNFLEQYDFFLFGFYATYIAAKFFHSENEYVSLMMTFTVFAAGFLMRPLGAIFLGAYVDRV
GRRKGLIITLSLMALGTILITFVPSYDAIGIIAPILVVIGRLLQGFSAGVESGGVSIYLAEIATDKNRGFITSWQSGSQQ
IAVVFAALLGYWLNSVLSHAQVSDWGWRIPFLIGCLIIPLIFVFRRTLEETEDFKAQKTHPTTKEIFSTLASNWRIVLAG
MMMSAMTTTTFYFITVYTTVYAKRTLEMSITDSLLATVFVGLSNFFWLPMGGLLSDKIGRRPVLVGITTLAIFTSYPVLS
WLVSDISFTNLIIALAYFSFFFGLYNGTMVATLAEVMPKRVRTVGFSLAFSLAAAIFGGMTPMACTFLVEHTGNPSTPAF
WLMFAAVCSLISSLYLCRGAKKNNPDAVVEPAKLSV

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=434, Percent_Identity=32.4884792626728, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI1788942, Length=424, Percent_Identity=33.2547169811321, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788292, Length=423, Percent_Identity=25.531914893617, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI1789941, Length=388, Percent_Identity=27.5773195876289, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87082231, Length=240, Percent_Identity=26.6666666666667, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48087; Mature: 48087

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQVAKRSATFKKILNVTSGNFLEQYDFFLFGFYATYIAAKFFHSENEYVSLMMTFTVFA
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
AGFLMRPLGAIFLGAYVDRVGRRKGLIITLSLMALGTILITFVPSYDAIGIIAPILVVIG
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RLLQGFSAGVESGGVSIYLAEIATDKNRGFITSWQSGSQQIAVVFAALLGYWLNSVLSHA
HHHHHHHCCCCCCCCEEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
QVSDWGWRIPFLIGCLIIPLIFVFRRTLEETEDFKAQKTHPTTKEIFSTLASNWRIVLAG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHH
MMMSAMTTTTFYFITVYTTVYAKRTLEMSITDSLLATVFVGLSNFFWLPMGGLLSDKIGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
RPVLVGITTLAIFTSYPVLSWLVSDISFTNLIIALAYFSFFFGLYNGTMVATLAEVMPKR
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VRTVGFSLAFSLAAAIFGGMTPMACTFLVEHTGNPSTPAFWLMFAAVCSLISSLYLCRGA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
KKNNPDAVVEPAKLSV
CCCCCCCEECCCCCCC
>Mature Secondary Structure
MEQVAKRSATFKKILNVTSGNFLEQYDFFLFGFYATYIAAKFFHSENEYVSLMMTFTVFA
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
AGFLMRPLGAIFLGAYVDRVGRRKGLIITLSLMALGTILITFVPSYDAIGIIAPILVVIG
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RLLQGFSAGVESGGVSIYLAEIATDKNRGFITSWQSGSQQIAVVFAALLGYWLNSVLSHA
HHHHHHHCCCCCCCCEEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
QVSDWGWRIPFLIGCLIIPLIFVFRRTLEETEDFKAQKTHPTTKEIFSTLASNWRIVLAG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHH
MMMSAMTTTTFYFITVYTTVYAKRTLEMSITDSLLATVFVGLSNFFWLPMGGLLSDKIGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
RPVLVGITTLAIFTSYPVLSWLVSDISFTNLIIALAYFSFFFGLYNGTMVATLAEVMPKR
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VRTVGFSLAFSLAAAIFGGMTPMACTFLVEHTGNPSTPAFWLMFAAVCSLISSLYLCRGA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
KKNNPDAVVEPAKLSV
CCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]