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The map label for this gene is nac [H]

Identifier: 50084708

GI number: 50084708

Start: 1544040

End: 1544969

Strand: Reverse

Name: nac [H]

Synonym: ACIAD1543

Alternate gene names: 50084708

Gene position: 1544969-1544040 (Counterclockwise)

Preceding gene: 50084709

Following gene: 50084706

Centisome position: 42.93

GC content: 43.23

Gene sequence:

>930_bases
ATGGAAATCAAACAAATCAAATATTTTTCAACCGTGGTTGAGGCTGGCAGTATTGGTAAAGCCGCTCAAAAGTTAGATGT
CGGTGCTTCTGCCATTAGTCAGCAAATTACCAAGCTAGAACAAGAGCTCTGCATTCGTTTATTACAACGTAATGCATTTG
GTGTGACCCCAACCCCCGCGGGTCTTGCTTTTTTAAAACACTCTCAATTAATTTTGCGTCAGATTAATTTTGCGATTGAT
GCAGCGCATTCAGCACGTCTGTCTGGTCATGTGAGTTTGGGTTTTCCGCCAACCATCAGTGCATTGATTGGCATCCCTTT
AATGAAATTAATGTCTGAACGCTACCCGGATATTCGCCTAGAAATTGTCGAAACGCTGTCAGGCAATCTGATTCAGTCAA
TTAATTCGCGCCAGCTTGATATAGCCATTATTTTTACTCATGAAATTGATAAGCAATGGGCCATTCAGCCCTTGGTTAAA
GAGCAAATGTATCTGATGGCACGAAAAGAAGTGCTGATCAAACATGGTTTGTCGGATTGTTTGCAAACGGGCATTATTCA
GTCACAGCAAATTGGGGATATTCCACTGGTCCTCCCCCGACAGCGCCACAGTTTACGTAAACTACTCGAACACAAAATTG
GTCAACTCAATGTGGTGTATGAGATTGATGGCCTACACCTGCTCATGGATAGCCTGATTCATCTAGATCTGGCCAGTGTC
CGGCCAGGAAGTGCTTGTTTACAGAAATACAAGCACCAGATCCAGCTCTTAAAAGTGATTGATCCTGAAATCGAGCGAAT
TAATTATCTGGTCAGTCTGGCAGAAGAAGAGCTTTCGCCAGCAGCACTGGCAGCCAAATCAGCCATTAAAGCCTGTGTAA
AAGAATTAATTCGGGCACAAATCTGGCCGTGTTCCGAACTCATTTTATAA

Upstream 100 bases:

>100_bases
CCACTTAAACATTTTTCTAGCAATATTGATAACATCTACTTTATTGGTTAACTTGTTTTATAGAATCATCTAGGGCACAG
GAAGAAAAGAATACATTCGA

Downstream 100 bases:

>100_bases
AGGGTCATTAATTTTTTTAAATACCCAATTACTTTTAGACTCTCACTCCTTTTCTGTTTTTCCTCGTACATTCCTGCCAC
ATGATAAATGAATTGTTCTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MEIKQIKYFSTVVEAGSIGKAAQKLDVGASAISQQITKLEQELCIRLLQRNAFGVTPTPAGLAFLKHSQLILRQINFAID
AAHSARLSGHVSLGFPPTISALIGIPLMKLMSERYPDIRLEIVETLSGNLIQSINSRQLDIAIIFTHEIDKQWAIQPLVK
EQMYLMARKEVLIKHGLSDCLQTGIIQSQQIGDIPLVLPRQRHSLRKLLEHKIGQLNVVYEIDGLHLLMDSLIHLDLASV
RPGSACLQKYKHQIQLLKVIDPEIERINYLVSLAEEELSPAALAAKSAIKACVKELIRAQIWPCSELIL

Sequences:

>Translated_309_residues
MEIKQIKYFSTVVEAGSIGKAAQKLDVGASAISQQITKLEQELCIRLLQRNAFGVTPTPAGLAFLKHSQLILRQINFAID
AAHSARLSGHVSLGFPPTISALIGIPLMKLMSERYPDIRLEIVETLSGNLIQSINSRQLDIAIIFTHEIDKQWAIQPLVK
EQMYLMARKEVLIKHGLSDCLQTGIIQSQQIGDIPLVLPRQRHSLRKLLEHKIGQLNVVYEIDGLHLLMDSLIHLDLASV
RPGSACLQKYKHQIQLLKVIDPEIERINYLVSLAEEELSPAALAAKSAIKACVKELIRAQIWPCSELIL
>Mature_309_residues
MEIKQIKYFSTVVEAGSIGKAAQKLDVGASAISQQITKLEQELCIRLLQRNAFGVTPTPAGLAFLKHSQLILRQINFAID
AAHSARLSGHVSLGFPPTISALIGIPLMKLMSERYPDIRLEIVETLSGNLIQSINSRQLDIAIIFTHEIDKQWAIQPLVK
EQMYLMARKEVLIKHGLSDCLQTGIIQSQQIGDIPLVLPRQRHSLRKLLEHKIGQLNVVYEIDGLHLLMDSLIHLDLASV
RPGSACLQKYKHQIQLLKVIDPEIERINYLVSLAEEELSPAALAAKSAIKACVKELIRAQIWPCSELIL

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=229, Percent_Identity=33.1877729257642, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787601, Length=172, Percent_Identity=26.7441860465116, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789506, Length=247, Percent_Identity=27.1255060728745, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI157672245, Length=274, Percent_Identity=25.9124087591241, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786401, Length=177, Percent_Identity=27.1186440677966, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787879, Length=151, Percent_Identity=32.4503311258278, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790399, Length=167, Percent_Identity=25.1497005988024, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787806, Length=144, Percent_Identity=29.8611111111111, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788887, Length=155, Percent_Identity=27.741935483871, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34444; Mature: 34444

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKQIKYFSTVVEAGSIGKAAQKLDVGASAISQQITKLEQELCIRLLQRNAFGVTPTPA
CCCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
GLAFLKHSQLILRQINFAIDAAHSARLSGHVSLGFPPTISALIGIPLMKLMSERYPDIRL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCHHH
EIVETLSGNLIQSINSRQLDIAIIFTHEIDKQWAIQPLVKEQMYLMARKEVLIKHGLSDC
HHHHHHCCHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQTGIIQSQQIGDIPLVLPRQRHSLRKLLEHKIGQLNVVYEIDGLHLLMDSLIHLDLASV
HHHCCCCCCCCCCCCEECCCHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHCC
RPGSACLQKYKHQIQLLKVIDPEIERINYLVSLAEEELSPAALAAKSAIKACVKELIRAQ
CCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
IWPCSELIL
CCCHHHHCC
>Mature Secondary Structure
MEIKQIKYFSTVVEAGSIGKAAQKLDVGASAISQQITKLEQELCIRLLQRNAFGVTPTPA
CCCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
GLAFLKHSQLILRQINFAIDAAHSARLSGHVSLGFPPTISALIGIPLMKLMSERYPDIRL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCHHH
EIVETLSGNLIQSINSRQLDIAIIFTHEIDKQWAIQPLVKEQMYLMARKEVLIKHGLSDC
HHHHHHCCHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQTGIIQSQQIGDIPLVLPRQRHSLRKLLEHKIGQLNVVYEIDGLHLLMDSLIHLDLASV
HHHCCCCCCCCCCCCEECCCHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHCC
RPGSACLQKYKHQIQLLKVIDPEIERINYLVSLAEEELSPAALAAKSAIKACVKELIRAQ
CCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
IWPCSELIL
CCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9495755; 9097040; 9278503; 8529872 [H]