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The map label for this gene is benK

Identifier: 50084610

GI number: 50084610

Start: 1431562

End: 1432962

Strand: Direct

Name: benK

Synonym: ACIAD1434

Alternate gene names: 50084610

Gene position: 1431562-1432962 (Clockwise)

Preceding gene: 50084609

Following gene: 50084612

Centisome position: 39.78

GC content: 43.25

Gene sequence:

>1401_bases
ATGTCTAGAGAAATTAATGTCAATCAGATGATTGACGACTCAAAGCTCACGCCGTTTCATTGGCGAGTGATTATACTCAG
TACCCTCATTATTATTTTTGATGGCTACGATCTGGTAATTTACGGGGTGGCGCTACCGCTTCTGATGAAAGAGTGGGCCA
TTGATCCGGTTACCGCAGGCTTTATTGGCAGTATTGCATTGTTTGGTATGATGTTTGGTGCCCTGATTTTTGGCACCATC
GCCGACAAGCTTGAACATTTGGGTGTGAGCCGTAAAAAAGTCATTGCGGTCTGTATTATTTTATTTAGCTTGTGCACGGT
ATTGTGCGGTTTTTCTGAAACCACCACGCAATTTTCCATATTTCGCTTTTTGGCAGGTGTAGGCATTGGTGGTGTAATGC
CAAACGTAATTGCACTGGTCAGTGAGTACGCACCGAAAAAATTTAAAAGTTTTTTTGTAACCTTGATGTTTAGTGGTTAT
GCCATTGGCGGCATGACGGCAGCTTTTCTGGGGTCGATTTTAGTGCCGCTTTATGGCTGGAAAATCATGTTTATGATTGC
CGGAATTCCATTGGTGTTGTTATTGCCTCTCATGAAAGTTTTGCCTGAATCGATTGACTATTTGGTTCGCAAAAAGAAAG
ATGAAACGGTTCGTTTTATTATGACCAAAATGGTGCCGTCGTATCAGTATCAGCCCGATCATGTTTTTGTACTGAACTCA
TCGAATCAAAATCAGGCACAAGCGCCAGTCAAAATGATTTTTCAGGAACAGCGTGCGTTTAGTACGATGATGTTCTGGTG
CTCAATTTTTATGACACTGATTATGGTTTATGCACTGGGGAACTGGTTGCCAAAACTGATGATTGAAGCAGGTTATAACC
TCTCGAAGAGTCTCATTTTCTTGTTCTCTTTAAACGTGGGGGGAATGATCGGCTCTATTTTGGGTGGATATCTGGCGGAC
CGCTATAACGTCAAATTTGTGACCATGGGCTTGTTATTGCTGGGTGCGATTTCACTGAGTCTGTTGTCTTTCCAGTTTAG
CAGTGTGATTTTGTATATCTTAATTGCATGTGCGGGCGCAGCCTCAATTGGTGCTCAAATTATGCTGCTGGCCTATATGG
CAAAATTTTATGCGCCAAATGTTCGCTCTACTGGAATTGGTTGGGGGCTTGGCATGGGACGTGTCGGCGCAATTTTAGGG
CCAATCTTGACTGGGTGGTTATTGTCTTTGCAACTGCCGCACTTCTATAACTTTCTGGCACTGAGTATTCCTGCGGTTCT
TGGTATTGTCACGGTATTTTTAATCAATGACAGACGTATGTATCAACCTGAACCGATCAGCCCGATTGCCAATCAAAACG
ACACCACTACGGTGAAAGTGAACGAAGCAGTCTCGCATTAA

Upstream 100 bases:

>100_bases
GTCCGTTATGGAACGGATTTTAACGAAAATCGTTTATTTCTAACTTACACCAAAAAATTCTGAGTGCTTGGTGCTAAATC
CTTATGGATGGAGGAATGTT

Downstream 100 bases:

>100_bases
TCTAAAAAATTATAAAAACCCATCAGCACCTCACAGTCTGATGGGTTTTTTGTTACCAGTTTGGCGGCTCAGTAAAACCT
TCATAGGCATATATCTGGCG

Product: MFS family benzoate membrane transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSREINVNQMIDDSKLTPFHWRVIILSTLIIIFDGYDLVIYGVALPLLMKEWAIDPVTAGFIGSIALFGMMFGALIFGTI
ADKLEHLGVSRKKVIAVCIILFSLCTVLCGFSETTTQFSIFRFLAGVGIGGVMPNVIALVSEYAPKKFKSFFVTLMFSGY
AIGGMTAAFLGSILVPLYGWKIMFMIAGIPLVLLLPLMKVLPESIDYLVRKKKDETVRFIMTKMVPSYQYQPDHVFVLNS
SNQNQAQAPVKMIFQEQRAFSTMMFWCSIFMTLIMVYALGNWLPKLMIEAGYNLSKSLIFLFSLNVGGMIGSILGGYLAD
RYNVKFVTMGLLLLGAISLSLLSFQFSSVILYILIACAGAASIGAQIMLLAYMAKFYAPNVRSTGIGWGLGMGRVGAILG
PILTGWLLSLQLPHFYNFLALSIPAVLGIVTVFLINDRRMYQPEPISPIANQNDTTTVKVNEAVSH

Sequences:

>Translated_466_residues
MSREINVNQMIDDSKLTPFHWRVIILSTLIIIFDGYDLVIYGVALPLLMKEWAIDPVTAGFIGSIALFGMMFGALIFGTI
ADKLEHLGVSRKKVIAVCIILFSLCTVLCGFSETTTQFSIFRFLAGVGIGGVMPNVIALVSEYAPKKFKSFFVTLMFSGY
AIGGMTAAFLGSILVPLYGWKIMFMIAGIPLVLLLPLMKVLPESIDYLVRKKKDETVRFIMTKMVPSYQYQPDHVFVLNS
SNQNQAQAPVKMIFQEQRAFSTMMFWCSIFMTLIMVYALGNWLPKLMIEAGYNLSKSLIFLFSLNVGGMIGSILGGYLAD
RYNVKFVTMGLLLLGAISLSLLSFQFSSVILYILIACAGAASIGAQIMLLAYMAKFYAPNVRSTGIGWGLGMGRVGAILG
PILTGWLLSLQLPHFYNFLALSIPAVLGIVTVFLINDRRMYQPEPISPIANQNDTTTVKVNEAVSH
>Mature_465_residues
SREINVNQMIDDSKLTPFHWRVIILSTLIIIFDGYDLVIYGVALPLLMKEWAIDPVTAGFIGSIALFGMMFGALIFGTIA
DKLEHLGVSRKKVIAVCIILFSLCTVLCGFSETTTQFSIFRFLAGVGIGGVMPNVIALVSEYAPKKFKSFFVTLMFSGYA
IGGMTAAFLGSILVPLYGWKIMFMIAGIPLVLLLPLMKVLPESIDYLVRKKKDETVRFIMTKMVPSYQYQPDHVFVLNSS
NQNQAQAPVKMIFQEQRAFSTMMFWCSIFMTLIMVYALGNWLPKLMIEAGYNLSKSLIFLFSLNVGGMIGSILGGYLADR
YNVKFVTMGLLLLGAISLSLLSFQFSSVILYILIACAGAASIGAQIMLLAYMAKFYAPNVRSTGIGWGLGMGRVGAILGP
ILTGWLLSLQLPHFYNFLALSIPAVLGIVTVFLINDRRMYQPEPISPIANQNDTTTVKVNEAVSH

Specific function: Probable uptake of benzoate

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Homologues:

Organism=Homo sapiens, GI24308167, Length=427, Percent_Identity=22.9508196721311, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI7662270, Length=247, Percent_Identity=23.8866396761134, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI73695465, Length=268, Percent_Identity=23.8805970149254, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI216548223, Length=256, Percent_Identity=23.046875, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI262399383, Length=192, Percent_Identity=24.4791666666667, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI213021148, Length=207, Percent_Identity=20.7729468599034, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI166064021, Length=348, Percent_Identity=21.8390804597701, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI87081723, Length=425, Percent_Identity=30.8235294117647, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI87082231, Length=427, Percent_Identity=24.1217798594848, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082159, Length=408, Percent_Identity=26.9607843137255, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786229, Length=243, Percent_Identity=27.1604938271605, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI87082404, Length=397, Percent_Identity=21.1586901763224, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788074, Length=375, Percent_Identity=24.8, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788068, Length=421, Percent_Identity=20.4275534441805, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787880, Length=174, Percent_Identity=27.0114942528736, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=421, Percent_Identity=22.5653206650831, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24640198, Length=477, Percent_Identity=24.1090146750524, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24640196, Length=477, Percent_Identity=24.1090146750524, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24640200, Length=477, Percent_Identity=24.1090146750524, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19922874, Length=202, Percent_Identity=24.7524752475248, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BENK_ACIAD (O30513)

Other databases:

- EMBL:   AF009224
- EMBL:   CR543861
- RefSeq:   YP_046120.1
- ProteinModelPortal:   O30513
- STRING:   O30513
- GeneID:   2880311
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1434
- NMPDR:   fig|62977.3.peg.793
- eggNOG:   COG0477
- HOGENOM:   HBG715489
- OMA:   SQILLYA
- ProtClustDB:   CLSK2762710
- BioCyc:   ASP62977:ACIAD1434-MONOMER
- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- TIGRFAMs:   TIGR00895

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 51403; Mature: 51272

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS50850 MFS; PS00216 SUGAR_TRANSPORT_1; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x16864e74)-; HASH(0x180fc130)-; HASH(0x180de144)-; HASH(0x17b45ac0)-; HASH(0x17c26398)-; HASH(0x17b3c95c)-; HASH(0x17c288c8)-; HASH(0x16c2a688)-; HASH(0x17dd0440)-; HASH(0x17c6e4fc)-; HASH(0x160da168)-; HASH(0x16ba0d00)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCH
>Mature Secondary Structure 
SREINVNQMIDDSKLTPFHWRVIILSTLIIIFDGYDLVIYGVALPLLMKEWAIDPVTAG
CCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHH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HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9294456