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The map label for this gene is benA

Identifier: 50084612

GI number: 50084612

Start: 1434223

End: 1435608

Strand: Direct

Name: benA

Synonym: ACIAD1436

Alternate gene names: 50084612

Gene position: 1434223-1435608 (Clockwise)

Preceding gene: 50084610

Following gene: 50084613

Centisome position: 39.85

GC content: 45.02

Gene sequence:

>1386_bases
ATGCCACGTATTCCCGTCATTAATACTAGCCATCTTGACCGAATTGATGAACTGCTTGTAGACAATACCGAAACAGGTGA
ATTTAAGTTACATCGTTCTGTATTTACAGATCAGGCACTTTTTGATCTTGAAATGAAATACATTTTCGAAGGAAATTGGG
TTTATTTGGCTCATGAAAGCCAGATTCCCAACAACAACGACTATTACACCACCTATATTGGCAGACAACCGATTTTGATT
GCGCGTAATCGCAACGGTGAACTCAACGCCATGATTAACGCATGTTCACATCGTGGTGCACAGCTGTGCCGTCATAAGCG
TGGTAATAAGACCACATATACTTGCCCATTTCATGGCTGGACCTTCAATAACTCAGGAAAATTGTTGAAGGTGAAAGATC
CAAGCGATGCTGGTTATTCAGATTGTTTTAATCAGGACGGTTCCCACGACTTAAAAAAGGTGGCGCGTTTTGAAAGTTAT
AAAGGTTTTTTATTTGGCAGTCTGAATCCTGATGTACCGTCACTGCAAGAGTTTTTGGGGGAAACCACCAAAATTATCGA
CATGATTGTCGGGCAATCCGATCAGGGCCTTGAAGTACTGCGTGGTGTTTCGACCTACACCTATGAAGGAAACTGGAAGT
TGACCGCAGAAAACGGAGCAGATGGCTATCATGTTTCGGCGGTGCACTGGAACTATGCAGCCACCACGCAGCATCGTAAA
GAAAAACAGGCAGGTGATACCATTCGCGCGATGAGCGCGGGCTCGTGGGGGAAACATGGTGGCGGTTCATATGGATTTGA
ACATGGTCATATGTTGCTCTGGACACAATGGGGTAATCCGGAAGACCGACCAAACTTTCCTAAAGCAGCGGAATATACCG
AAAAATTCGGTGCTGCAATGTCGAAATGGATGATCGAACGCTCACGTAACTTGTGTTTATATCCAAACGTGTACCTGATG
GATCAGTTTGGTTCGCAAATTCGTGTTTTACGTCCAATTTCGGTCAATAAAACCGAAGTCACCATTTACTGTATTGCGCC
TGTAGGTGAAGCACCCGAAGCGCGTGCACGCCGTATTCGCCAGTATGAAGATTTCTTTAATGCATCTGGAATGGCGACGC
CAGACGATCTTGAGGAGTTCCGTGCCTGTCAGGCTGGTTATGCAGGTATCGAACTGGAATGGAACGACATGTGCCGCGGA
TCAAAACATTGGATTTATGGACCAGATGATGCCGCTAATGAAATCGGATTAAAACCGGCTATTAGTGGTATTAAAACTGA
AGACGAAGGCTTGTATTTGGCACAGCATCAATACTGGCTCAAAAGTATGAAGCAAGCGATTGCTGCGGAAAAAGAATTTG
CATCGCGTCAGGGAGAGAACGCATGA

Upstream 100 bases:

>100_bases
ACATTTTATGCGTTGCGTTGTTTAATTGAATGTTTGACTAAGCACAGCGTTTTGCTCTGGCCTAGACAAGTTTCTTATTT
TGGAATGTTGGAGAAAGGAT

Downstream 100 bases:

>100_bases
ATGCTACAGCACTTTTAGACACCATCAGCATCGAACAGATTAGCCAGTTTTTGTATAGCGAGGCCCGTTTTTTAGATGAT
GAGCAATGGGATGACTGGCT

Product: benzoate 1,2-dioxygenase alpha subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MPRIPVINTSHLDRIDELLVDNTETGEFKLHRSVFTDQALFDLEMKYIFEGNWVYLAHESQIPNNNDYYTTYIGRQPILI
ARNRNGELNAMINACSHRGAQLCRHKRGNKTTYTCPFHGWTFNNSGKLLKVKDPSDAGYSDCFNQDGSHDLKKVARFESY
KGFLFGSLNPDVPSLQEFLGETTKIIDMIVGQSDQGLEVLRGVSTYTYEGNWKLTAENGADGYHVSAVHWNYAATTQHRK
EKQAGDTIRAMSAGSWGKHGGGSYGFEHGHMLLWTQWGNPEDRPNFPKAAEYTEKFGAAMSKWMIERSRNLCLYPNVYLM
DQFGSQIRVLRPISVNKTEVTIYCIAPVGEAPEARARRIRQYEDFFNASGMATPDDLEEFRACQAGYAGIELEWNDMCRG
SKHWIYGPDDAANEIGLKPAISGIKTEDEGLYLAQHQYWLKSMKQAIAAEKEFASRQGENA

Sequences:

>Translated_461_residues
MPRIPVINTSHLDRIDELLVDNTETGEFKLHRSVFTDQALFDLEMKYIFEGNWVYLAHESQIPNNNDYYTTYIGRQPILI
ARNRNGELNAMINACSHRGAQLCRHKRGNKTTYTCPFHGWTFNNSGKLLKVKDPSDAGYSDCFNQDGSHDLKKVARFESY
KGFLFGSLNPDVPSLQEFLGETTKIIDMIVGQSDQGLEVLRGVSTYTYEGNWKLTAENGADGYHVSAVHWNYAATTQHRK
EKQAGDTIRAMSAGSWGKHGGGSYGFEHGHMLLWTQWGNPEDRPNFPKAAEYTEKFGAAMSKWMIERSRNLCLYPNVYLM
DQFGSQIRVLRPISVNKTEVTIYCIAPVGEAPEARARRIRQYEDFFNASGMATPDDLEEFRACQAGYAGIELEWNDMCRG
SKHWIYGPDDAANEIGLKPAISGIKTEDEGLYLAQHQYWLKSMKQAIAAEKEFASRQGENA
>Mature_460_residues
PRIPVINTSHLDRIDELLVDNTETGEFKLHRSVFTDQALFDLEMKYIFEGNWVYLAHESQIPNNNDYYTTYIGRQPILIA
RNRNGELNAMINACSHRGAQLCRHKRGNKTTYTCPFHGWTFNNSGKLLKVKDPSDAGYSDCFNQDGSHDLKKVARFESYK
GFLFGSLNPDVPSLQEFLGETTKIIDMIVGQSDQGLEVLRGVSTYTYEGNWKLTAENGADGYHVSAVHWNYAATTQHRKE
KQAGDTIRAMSAGSWGKHGGGSYGFEHGHMLLWTQWGNPEDRPNFPKAAEYTEKFGAAMSKWMIERSRNLCLYPNVYLMD
QFGSQIRVLRPISVNKTEVTIYCIAPVGEAPEARARRIRQYEDFFNASGMATPDDLEEFRACQAGYAGIELEWNDMCRGS
KHWIYGPDDAANEIGLKPAISGIKTEDEGLYLAQHQYWLKSMKQAIAAEKEFASRQGENA

Specific function: Degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate (DHB)

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain

Homologues:

Organism=Escherichia coli, GI1788888, Length=380, Percent_Identity=27.3684210526316, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1788103, Length=204, Percent_Identity=30.3921568627451, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BENA_ACIAD (P07769)

Other databases:

- EMBL:   AF009224
- EMBL:   CR543861
- RefSeq:   YP_046122.1
- ProteinModelPortal:   P07769
- STRING:   P07769
- GeneID:   2880890
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1436
- NMPDR:   fig|62977.3.peg.794
- eggNOG:   COG4638
- HOGENOM:   HBG360459
- OMA:   NWKLQTE
- ProtClustDB:   CLSK862340
- BioCyc:   ASP62977:ACIAD1436-MONOMER
- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663
- Gene3D:   G3DSA:2.102.10.10
- PANTHER:   PTHR21266:SF2
- PRINTS:   PR00090
- TIGRFAMs:   TIGR03229

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A; SSF50022 Rieske_dom

EC number: =1.14.12.10

Molecular weight: Translated: 52229; Mature: 52098

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS51296 RIESKE; PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRIPVINTSHLDRIDELLVDNTETGEFKLHRSVFTDQALFDLEMKYIFEGNWVYLAHES
CCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHCCCHHEEEEEEEEEECCEEEEEECC
QIPNNNDYYTTYIGRQPILIARNRNGELNAMINACSHRGAQLCRHKRGNKTTYTCPFHGW
CCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCE
TFNNSGKLLKVKDPSDAGYSDCFNQDGSHDLKKVARFESYKGFLFGSLNPDVPSLQEFLG
EECCCCCEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHH
ETTKIIDMIVGQSDQGLEVLRGVSTYTYEGNWKLTAENGADGYHVSAVHWNYAATTQHRK
HHHHHHHHHHCCCCCCHHHHHCCEEEEECCCEEEEECCCCCCEEEEEEEECCHHHHHHHH
EKQAGDTIRAMSAGSWGKHGGGSYGFEHGHMLLWTQWGNPEDRPNFPKAAEYTEKFGAAM
HHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
SKWMIERSRNLCLYPNVYLMDQFGSQIRVLRPISVNKTEVTIYCIAPVGEAPEARARRIR
HHHHHHHCCCEEECCCEEEECCCCCCEEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHH
QYEDFFNASGMATPDDLEEFRACQAGYAGIELEWNDMCRGSKHWIYGPDDAANEIGLKPA
HHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCEEECCCCCCHHCCCCHH
ISGIKTEDEGLYLAQHQYWLKSMKQAIAAEKEFASRQGENA
HCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PRIPVINTSHLDRIDELLVDNTETGEFKLHRSVFTDQALFDLEMKYIFEGNWVYLAHES
CCCCCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHCCCHHEEEEEEEEEECCEEEEEECC
QIPNNNDYYTTYIGRQPILIARNRNGELNAMINACSHRGAQLCRHKRGNKTTYTCPFHGW
CCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCE
TFNNSGKLLKVKDPSDAGYSDCFNQDGSHDLKKVARFESYKGFLFGSLNPDVPSLQEFLG
EECCCCCEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHH
ETTKIIDMIVGQSDQGLEVLRGVSTYTYEGNWKLTAENGADGYHVSAVHWNYAATTQHRK
HHHHHHHHHHCCCCCCHHHHHCCEEEEECCCEEEEECCCCCCEEEEEEEECCHHHHHHHH
EKQAGDTIRAMSAGSWGKHGGGSYGFEHGHMLLWTQWGNPEDRPNFPKAAEYTEKFGAAM
HHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
SKWMIERSRNLCLYPNVYLMDQFGSQIRVLRPISVNKTEVTIYCIAPVGEAPEARARRIR
HHHHHHHCCCEEECCCEEEECCCCCCEEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHH
QYEDFFNASGMATPDDLEEFRACQAGYAGIELEWNDMCRGSKHWIYGPDDAANEIGLKPA
HHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCEEECCCCCCHHCCCCHH
ISGIKTEDEGLYLAQHQYWLKSMKQAIAAEKEFASRQGENA
HCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885518