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The map label for this gene is recA

Identifier: 50084563

GI number: 50084563

Start: 1380692

End: 1381741

Strand: Reverse

Name: recA

Synonym: ACIAD1385

Alternate gene names: 50084563

Gene position: 1381741-1380692 (Counterclockwise)

Preceding gene: 50084564

Following gene: 50084562

Centisome position: 38.4

GC content: 40.57

Gene sequence:

>1050_bases
ATGGATGATAATAAAAGCAAGGCATTACAAGCTGCTTTAAGTCAGATTGAAAAACAATTTGGCAAAAATACAGTAATGCG
TTTAGGTGATAATACCGTTCAAGCTGTTGAAGCCGTTTCGACAGGCTCTCTTACTCTAGATATTGCATTAGGTATTGGTG
GCTTACCTAAAGGGCGTATTGTAGAGATTTACGGTCCTGAATCTTCTGGTAAAACAACCATGACTTTGCAAGCAATTGCA
CAATGTCAGAAAGCAGGTGGTACCTGTGCTTTTATTGATGCAGAACATGCATTAGACCCTCAGTATGCCCGTAAGCTGGG
TGTAGACATAGACAATCTTTTGGTATCGCAGCCTGATCATGGTGAACAAGCTCTGGAAATTGCAGATATGCTTGTACGCT
CTGGTGCGATTGATCTGATTGTAGTCGACTCTGTAGCAGCATTAACCCCAAAAGCCGAAATTGAAGGTGAAATGGGCGAC
TCCCACATGGGTTTACAAGCCCGCTTAATGAGTCAAGCCTTACGTAAAATCACGGGTAATGCTAAACGTTCGAATTGCAT
GGTAATCTTCATTAACCAGATTCGTATGAAAATTGGCGTAATGTTTGGCAGCCCAGAAACCACAACTGGTGGTAATGCAC
TAAAATTCTATGCATCTGTACGTCTAGATATACGCCGTATTGGTCAAGTTAAAGAAGGTGATGAAATCATTGGTTCTGAA
ACCAAAGTCAAAGTCGTTAAAAATAAAATGGCCCCTCCTTTCCGTGAAGCAATCTTCCAAATTTTATATGGCAAGGGTGT
CAATCAACTTGGCGAATTGGTTGATCTTGCAGTTCAACAAAATATTGTACAAAAAGCTGGTGCTTGGTATTCATACCAAG
GCAATAAAATTGGTCAGGGTAAGAATAACGTGATTCGTTATCTTGAAGAGAACCCTCAGATTTCTACAGAAATTGAAGCG
GTTATCCGTGAACAATTATTGACTAAGGCATCAGATCAGACCGCTGCTCACGATGAAACTGAAGAAGAACCAGATTTACT
GGAAAGTTAA

Upstream 100 bases:

>100_bases
AGTGAAAATAAATCTGTCACTATACTTCGGTGGAACAAGGTTGAAATTCAATACATCCACTTTGAATGAAAAAGTGATAT
TACAGTTTTGAGGTTGTAAG

Downstream 100 bases:

>100_bases
TCTAAGCATCCAATTAAACGCCCAAATGGGCGTTTTTTCATTTGAAAGGCTATCTAGATGATATTTAAGCATACTCAGAC
ATCGCGTGCGGAGCTATCTG

Product: recombinase A

Products: NA

Alternate protein names: Recombinase A

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MDDNKSKALQAALSQIEKQFGKNTVMRLGDNTVQAVEAVSTGSLTLDIALGIGGLPKGRIVEIYGPESSGKTTMTLQAIA
QCQKAGGTCAFIDAEHALDPQYARKLGVDIDNLLVSQPDHGEQALEIADMLVRSGAIDLIVVDSVAALTPKAEIEGEMGD
SHMGLQARLMSQALRKITGNAKRSNCMVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRIGQVKEGDEIIGSE
TKVKVVKNKMAPPFREAIFQILYGKGVNQLGELVDLAVQQNIVQKAGAWYSYQGNKIGQGKNNVIRYLEENPQISTEIEA
VIREQLLTKASDQTAAHDETEEEPDLLES

Sequences:

>Translated_349_residues
MDDNKSKALQAALSQIEKQFGKNTVMRLGDNTVQAVEAVSTGSLTLDIALGIGGLPKGRIVEIYGPESSGKTTMTLQAIA
QCQKAGGTCAFIDAEHALDPQYARKLGVDIDNLLVSQPDHGEQALEIADMLVRSGAIDLIVVDSVAALTPKAEIEGEMGD
SHMGLQARLMSQALRKITGNAKRSNCMVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRIGQVKEGDEIIGSE
TKVKVVKNKMAPPFREAIFQILYGKGVNQLGELVDLAVQQNIVQKAGAWYSYQGNKIGQGKNNVIRYLEENPQISTEIEA
VIREQLLTKASDQTAAHDETEEEPDLLES
>Mature_349_residues
MDDNKSKALQAALSQIEKQFGKNTVMRLGDNTVQAVEAVSTGSLTLDIALGIGGLPKGRIVEIYGPESSGKTTMTLQAIA
QCQKAGGTCAFIDAEHALDPQYARKLGVDIDNLLVSQPDHGEQALEIADMLVRSGAIDLIVVDSVAALTPKAEIEGEMGD
SHMGLQARLMSQALRKITGNAKRSNCMVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRIGQVKEGDEIIGSE
TKVKVVKNKMAPPFREAIFQILYGKGVNQLGELVDLAVQQNIVQKAGAWYSYQGNKIGQGKNNVIRYLEENPQISTEIEA
VIREQLLTKASDQTAAHDETEEEPDLLES

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family

Homologues:

Organism=Escherichia coli, GI1789051, Length=328, Percent_Identity=73.780487804878, Blast_Score=509, Evalue=1e-146,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RECA_ACIAD (P42438)

Other databases:

- EMBL:   L26100
- EMBL:   CR543861
- PIR:   I39509
- RefSeq:   YP_046073.1
- ProteinModelPortal:   P42438
- SMR:   P42438
- STRING:   P42438
- GeneID:   2879476
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1385
- NMPDR:   fig|62977.3.peg.687
- eggNOG:   COG0468
- HOGENOM:   HBG339889
- OMA:   CEYLREN
- PhylomeDB:   P42438
- ProtClustDB:   PRK09354
- BioCyc:   ASP62977:ACIAD1385-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00268
- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587
- PANTHER:   PTHR22942:SF1
- PRINTS:   PR00142
- SMART:   SM00382
- TIGRFAMs:   TIGR02012

Pfam domain/function: PF00154 RecA

EC number: NA

Molecular weight: Translated: 37816; Mature: 37816

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00321 RECA_1; PS50162 RECA_2; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDNKSKALQAALSQIEKQFGKNTVMRLGDNTVQAVEAVSTGSLTLDIALGIGGLPKGRI
CCCCHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCE
VEIYGPESSGKTTMTLQAIAQCQKAGGTCAFIDAEHALDPQYARKLGVDIDNLLVSQPDH
EEEECCCCCCCEEHHHHHHHHHHHCCCEEEEEECHHCCCHHHHHHHCCCHHHHHCCCCCC
GEQALEIADMLVRSGAIDLIVVDSVAALTPKAEIEGEMGDSHMGLQARLMSQALRKITGN
CHHHHHHHHHHHHCCCEEEEEECCHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
AKRSNCMVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRIGQVKEGDEIIGSE
CCCCCEEEEEEEHEEEEEEEEECCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCHHHCCC
TKVKVVKNKMAPPFREAIFQILYGKGVNQLGELVDLAVQQNIVQKAGAWYSYQGNKIGQG
HHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCC
KNNVIRYLEENPQISTEIEAVIREQLLTKASDQTAAHDETEEEPDLLES
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCC
>Mature Secondary Structure
MDDNKSKALQAALSQIEKQFGKNTVMRLGDNTVQAVEAVSTGSLTLDIALGIGGLPKGRI
CCCCHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCE
VEIYGPESSGKTTMTLQAIAQCQKAGGTCAFIDAEHALDPQYARKLGVDIDNLLVSQPDH
EEEECCCCCCCEEHHHHHHHHHHHCCCEEEEEECHHCCCHHHHHHHCCCHHHHHCCCCCC
GEQALEIADMLVRSGAIDLIVVDSVAALTPKAEIEGEMGDSHMGLQARLMSQALRKITGN
CHHHHHHHHHHHHCCCEEEEEECCHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
AKRSNCMVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRIGQVKEGDEIIGSE
CCCCCEEEEEEEHEEEEEEEEECCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCHHHCCC
TKVKVVKNKMAPPFREAIFQILYGKGVNQLGELVDLAVQQNIVQKAGAWYSYQGNKIGQG
HHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCC
KNNVIRYLEENPQISTEIEAVIREQLLTKASDQTAAHDETEEEPDLLES
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934905