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The map label for this gene is pyrB

Identifier: 50084456

GI number: 50084456

Start: 1270608

End: 1271624

Strand: Direct

Name: pyrB

Synonym: ACIAD1270

Alternate gene names: 50084456

Gene position: 1270608-1271624 (Clockwise)

Preceding gene: 50084455

Following gene: 50084457

Centisome position: 35.31

GC content: 43.17

Gene sequence:

>1017_bases
ATGCACTTGGCTGCATTGCATACTCCGAGTCAGATTCAGCTCAACCAACAGGGCAATTTAAAACACTTTTTGACCATCGA
AGGTCTTTCTAAAGAAACACTTACAAAAATTCTCGATACTGCACAATCCTTCATTAATGATCAAAACCAGCTCATCACCA
CGCCATTACTCGAAGGGCGCACGGTGATGAATCTGTTTTTTGAAAACTCAACTCGTACCCGAACAACCTTTGAAGCTGCG
GCAAAACGTTTGTCTGCCAATGTTTTAAATATTGATATTGCTCGTTCAAGTACCTCTAAGGGTGAAACCCTCCGTGACAC
GCTTTGGAATCTAGAAGCGATGGCTGCCGATATTTTTGTGGTACGTCATTCTTCTTCGGGTGCTGCACATTTTATAGCGA
AAGATGTATGCCCGCACGTGGCAATTATCAATGCTGGAGATGGACGTCATGCCCATCCAACGCAAGCGATGTTAGATATG
CTCACTATTCGTCGTGAGACCAAAAAGCCATTTGAAGAATTGAGTGTTGCAATTATTGGTGATATTAAACATTCGCGTGT
GGCACGTTCAAACATTGCCGCCTTGCAGACCTTGGGCTGTAACGATATTCGTGTGATTGCACCCAATACTTTATTGCCTG
TGGGTTTTAAAGAGTATGGTGACCATGTACGTCTATTTAATAAAATGGATGATGGTGTCAAAGATTGCGATGTGATTATC
GCGCTACGTATTCAAAATGAGCGTATTGATTCTCCTGCATTGTCTTCGCAGGCAGAATTTTATCGTATGTATGGTTTGAA
TCAGGAACGTTTGGCATTGGCGAAACCCGATTGTATCGTGATGCATCCAGGACCCATGAACCGTGGTGTTGAAATTGATT
CAAGCATTGCCGATGGTCCTCAGTCTGTTATTTTGCATCAGGTGACCAATGGGATCGCGGTACGAATGGCAGTATTGGCG
CTTTCAATGCAGGGTCAGTTACAAGAACAGGGTTTAATTGAAGCAATTGCAGTATAA

Upstream 100 bases:

>100_bases
CCATCTTTGACTGAGAAAACAGCAATTAACCAAAACTTCAACTGCCTGATCCAGAAAAATATCTGTATAATGCGTTAACT
TAACGTATAAGGAATCTCTC

Downstream 100 bases:

>100_bases
GGGATCGAAATCATGAGCAATATCAAACTTGAAAATGTCCGCTTACTCGATCCAGTTGCAAAAATTGATCAGGTCACGAC
GATTTATTTAAAAGATGGTA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MHLAALHTPSQIQLNQQGNLKHFLTIEGLSKETLTKILDTAQSFINDQNQLITTPLLEGRTVMNLFFENSTRTRTTFEAA
AKRLSANVLNIDIARSSTSKGETLRDTLWNLEAMAADIFVVRHSSSGAAHFIAKDVCPHVAIINAGDGRHAHPTQAMLDM
LTIRRETKKPFEELSVAIIGDIKHSRVARSNIAALQTLGCNDIRVIAPNTLLPVGFKEYGDHVRLFNKMDDGVKDCDVII
ALRIQNERIDSPALSSQAEFYRMYGLNQERLALAKPDCIVMHPGPMNRGVEIDSSIADGPQSVILHQVTNGIAVRMAVLA
LSMQGQLQEQGLIEAIAV

Sequences:

>Translated_338_residues
MHLAALHTPSQIQLNQQGNLKHFLTIEGLSKETLTKILDTAQSFINDQNQLITTPLLEGRTVMNLFFENSTRTRTTFEAA
AKRLSANVLNIDIARSSTSKGETLRDTLWNLEAMAADIFVVRHSSSGAAHFIAKDVCPHVAIINAGDGRHAHPTQAMLDM
LTIRRETKKPFEELSVAIIGDIKHSRVARSNIAALQTLGCNDIRVIAPNTLLPVGFKEYGDHVRLFNKMDDGVKDCDVII
ALRIQNERIDSPALSSQAEFYRMYGLNQERLALAKPDCIVMHPGPMNRGVEIDSSIADGPQSVILHQVTNGIAVRMAVLA
LSMQGQLQEQGLIEAIAV
>Mature_338_residues
MHLAALHTPSQIQLNQQGNLKHFLTIEGLSKETLTKILDTAQSFINDQNQLITTPLLEGRTVMNLFFENSTRTRTTFEAA
AKRLSANVLNIDIARSSTSKGETLRDTLWNLEAMAADIFVVRHSSSGAAHFIAKDVCPHVAIINAGDGRHAHPTQAMLDM
LTIRRETKKPFEELSVAIIGDIKHSRVARSNIAALQTLGCNDIRVIAPNTLLPVGFKEYGDHVRLFNKMDDGVKDCDVII
ALRIQNERIDSPALSSQAEFYRMYGLNQERLALAKPDCIVMHPGPMNRGVEIDSSIADGPQSVILHQVTNGIAVRMAVLA
LSMQGQLQEQGLIEAIAV

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=308, Percent_Identity=32.4675324675325, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI2367364, Length=315, Percent_Identity=34.6031746031746, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1786469, Length=334, Percent_Identity=22.4550898203593, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=348, Percent_Identity=32.7586206896552, Blast_Score=135, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6322331, Length=317, Percent_Identity=32.807570977918, Blast_Score=137, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24642586, Length=319, Percent_Identity=34.4827586206897, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_ACIAD (Q6FCR5)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045966.1
- ProteinModelPortal:   Q6FCR5
- SMR:   Q6FCR5
- STRING:   Q6FCR5
- GeneID:   2879563
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1270
- NMPDR:   fig|62977.3.peg.395
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- PhylomeDB:   Q6FCR5
- ProtClustDB:   PRK00856
- BioCyc:   ASP62977:ACIAD1270-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 37189; Mature: 37189

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLAALHTPSQIQLNQQGNLKHFLTIEGLSKETLTKILDTAQSFINDQNQLITTPLLEGR
CCEEEECCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCC
TVMNLFFENSTRTRTTFEAAAKRLSANVLNIDIARSSTSKGETLRDTLWNLEAMAADIFV
EEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHEEEEEE
VRHSSSGAAHFIAKDVCPHVAIINAGDGRHAHPTQAMLDMLTIRRETKKPFEELSVAIIG
EEECCCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCEEEEE
DIKHSRVARSNIAALQTLGCNDIRVIAPNTLLPVGFKEYGDHVRLFNKMDDGVKDCDVII
CCHHHHHHHHHHHHHHHCCCCCEEEECCCCEECCCHHHHCCHHHHHHHHCCCCCCCEEEE
ALRIQNERIDSPALSSQAEFYRMYGLNQERLALAKPDCIVMHPGPMNRGVEIDSSIADGP
EEEEECCCCCCCCCCHHHHHHHHHCCCHHHEEEECCCEEEECCCCCCCCEEECCCCCCCH
QSVILHQVTNGIAVRMAVLALSMQGQLQEQGLIEAIAV
HHHHHHHHHCHHHHHHHHHHHHHCCCHHHCCHHHHHCC
>Mature Secondary Structure
MHLAALHTPSQIQLNQQGNLKHFLTIEGLSKETLTKILDTAQSFINDQNQLITTPLLEGR
CCEEEECCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCC
TVMNLFFENSTRTRTTFEAAAKRLSANVLNIDIARSSTSKGETLRDTLWNLEAMAADIFV
EEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHEEEEEE
VRHSSSGAAHFIAKDVCPHVAIINAGDGRHAHPTQAMLDMLTIRRETKKPFEELSVAIIG
EEECCCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCEEEEE
DIKHSRVARSNIAALQTLGCNDIRVIAPNTLLPVGFKEYGDHVRLFNKMDDGVKDCDVII
CCHHHHHHHHHHHHHHHCCCCCEEEECCCCEECCCHHHHCCHHHHHHHHCCCCCCCEEEE
ALRIQNERIDSPALSSQAEFYRMYGLNQERLALAKPDCIVMHPGPMNRGVEIDSSIADGP
EEEEECCCCCCCCCCHHHHHHHHHCCCHHHEEEECCCEEEECCCCCCCCEEECCCCCCCH
QSVILHQVTNGIAVRMAVLALSMQGQLQEQGLIEAIAV
HHHHHHHHHCHHHHHHHHHHHHHCCCHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA