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The map label for this gene is pyrX [H]

Identifier: 50084457

GI number: 50084457

Start: 1271637

End: 1272872

Strand: Direct

Name: pyrX [H]

Synonym: ACIAD1271

Alternate gene names: 50084457

Gene position: 1271637-1272872 (Clockwise)

Preceding gene: 50084456

Following gene: 50084458

Centisome position: 35.34

GC content: 43.93

Gene sequence:

>1236_bases
ATGAGCAATATCAAACTTGAAAATGTCCGCTTACTCGATCCAGTTGCAAAAATTGATCAGGTCACGACGATTTATTTAAA
AGATGGTAAGCTGGTTGAATCTCTTGATCAGGTTGATGAAACCATCAATGCAGATGGTCAATGGCTAATGCCAACGATGG
TTGATTTATGCGCGCGTGTACGCGAGCCAGGGCAGCAACAGCACGGTACATTGAAATCTGAAGGTAAAGCCGCTCGCGAA
AATGGTATCTTGCATGTCATTACGCCACCAGACTCAAAACCGATTGTTCAAGATAATGGTGCGTTGATTCATGGTTTAAT
TGAAAAAGCATGGACGGACGGCCAGTTTCACTTGCATATTATTGGCGCACAAACTCAGGGATTATTGGGCAGACAGCCTG
CCAATATGGCTGCGCTCAAAAAAGGTGGTTGTACCGCAGTTTCTAATGCCAATGCCTCATTTGAAGATGATGATGTCGTC
ATTCGTACCCTAGAATATGCAGCAGGTTTGGGTTTAACCGTGGTGTTTTATCCTGAAGAAGCGCAAATTGCCAAAGATGG
CTGTGTACATGAAGGGTTTATTGCTTCGCGTCAAGGTTTACCGATGATTCCTGCAATTGCAGAAACCATTGCGATTGCTA
AATATCTGATCATGATTGAAGCCACAGGCGTGCGTGCGCACTTTGGTCTCATCTCTTCTGGAAAATCCATTGAGCTGATA
CAGGCTGCCAAAGCCAAGGGGCTACCTGTAACGGCAGATGTGGCAATGCATCAGCTACATTTGACAGAATCATTGATTGA
CGGTTTTAACTCATTGGCACATGTGCGTCCACCATTGCGATCTGAGCAAGACAAAGAGCAATTACGTCAGGCACTCAAGT
CGGGTGTGATTGATGCAATTTGTACGCATCATGAACCTCTAAGTAGCTCTGCGAAAATGATGCCGTTTGCAGAAACGCTC
CCAGGAATTAGTGCCTTCGATACCTATGTTGCATTGGGCATTCAACTGGTGAGGGAAGCGCTATTTACACCACTTGAATG
GGTTGAAAAAGTAACGTTGATACCTGCACAAGTTGCCAATATGCAAGAGCGCTGGCTCGATGAAAAAGGCTGGGTATTGG
TCGATCCAGATTTAGAATGGAGCGTAGATAAGAAAAGTATTTTGTCTAGTGGCAAAAATACACCACTTTTAAATCAGACC
TTAACGGGTAAAGTCATCAAAACATTTTTGATTTAA

Upstream 100 bases:

>100_bases
TGGGATCGCGGTACGAATGGCAGTATTGGCGCTTTCAATGCAGGGTCAGTTACAAGAACAGGGTTTAATTGAAGCAATTG
CAGTATAAGGGATCGAAATC

Downstream 100 bases:

>100_bases
ATTTTAAATCAGTTAAAAACTCGGCAAAATCATTGTCGAGTTTTTTTATTAAACTCATGTGTAAATATAAAATAAAAACT
CGATTTTTAATGATAAAGAT

Product: aspartate carbamoyltransferase

Products: N-carbamoyl-L-aspartate

Alternate protein names: Aspartate carbamoyltransferase 44 kDa Non-catalytic chain [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MSNIKLENVRLLDPVAKIDQVTTIYLKDGKLVESLDQVDETINADGQWLMPTMVDLCARVREPGQQQHGTLKSEGKAARE
NGILHVITPPDSKPIVQDNGALIHGLIEKAWTDGQFHLHIIGAQTQGLLGRQPANMAALKKGGCTAVSNANASFEDDDVV
IRTLEYAAGLGLTVVFYPEEAQIAKDGCVHEGFIASRQGLPMIPAIAETIAIAKYLIMIEATGVRAHFGLISSGKSIELI
QAAKAKGLPVTADVAMHQLHLTESLIDGFNSLAHVRPPLRSEQDKEQLRQALKSGVIDAICTHHEPLSSSAKMMPFAETL
PGISAFDTYVALGIQLVREALFTPLEWVEKVTLIPAQVANMQERWLDEKGWVLVDPDLEWSVDKKSILSSGKNTPLLNQT
LTGKVIKTFLI

Sequences:

>Translated_411_residues
MSNIKLENVRLLDPVAKIDQVTTIYLKDGKLVESLDQVDETINADGQWLMPTMVDLCARVREPGQQQHGTLKSEGKAARE
NGILHVITPPDSKPIVQDNGALIHGLIEKAWTDGQFHLHIIGAQTQGLLGRQPANMAALKKGGCTAVSNANASFEDDDVV
IRTLEYAAGLGLTVVFYPEEAQIAKDGCVHEGFIASRQGLPMIPAIAETIAIAKYLIMIEATGVRAHFGLISSGKSIELI
QAAKAKGLPVTADVAMHQLHLTESLIDGFNSLAHVRPPLRSEQDKEQLRQALKSGVIDAICTHHEPLSSSAKMMPFAETL
PGISAFDTYVALGIQLVREALFTPLEWVEKVTLIPAQVANMQERWLDEKGWVLVDPDLEWSVDKKSILSSGKNTPLLNQT
LTGKVIKTFLI
>Mature_410_residues
SNIKLENVRLLDPVAKIDQVTTIYLKDGKLVESLDQVDETINADGQWLMPTMVDLCARVREPGQQQHGTLKSEGKAAREN
GILHVITPPDSKPIVQDNGALIHGLIEKAWTDGQFHLHIIGAQTQGLLGRQPANMAALKKGGCTAVSNANASFEDDDVVI
RTLEYAAGLGLTVVFYPEEAQIAKDGCVHEGFIASRQGLPMIPAIAETIAIAKYLIMIEATGVRAHFGLISSGKSIELIQ
AAKAKGLPVTADVAMHQLHLTESLIDGFNSLAHVRPPLRSEQDKEQLRQALKSGVIDAICTHHEPLSSSAKMMPFAETLP
GISAFDTYVALGIQLVREALFTPLEWVEKVTLIPAQVANMQERWLDEKGWVLVDPDLEWSVDKKSILSSGKNTPLLNQTL
TGKVIKTFLI

Specific function: Non-functional DHOase [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. PyrC' subfamily [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=367, Percent_Identity=25.8855585831063, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1786722, Length=415, Percent_Identity=21.4457831325301, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI193204318, Length=360, Percent_Identity=25, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6322218, Length=194, Percent_Identity=28.3505154639175, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24642586, Length=205, Percent_Identity=26.3414634146341, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004722
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.3

Molecular weight: Translated: 44761; Mature: 44630

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIKLENVRLLDPVAKIDQVTTIYLKDGKLVESLDQVDETINADGQWLMPTMVDLCARV
CCCCEECCEEECCHHHHHCCEEEEEEECCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHH
REPGQQQHGTLKSEGKAARENGILHVITPPDSKPIVQDNGALIHGLIEKAWTDGQFHLHI
HCCCHHHCCCCCCCCCHHHHCCEEEEECCCCCCCEECCCCHHHHHHHHHHCCCCEEEEEE
IGAQTQGLLGRQPANMAALKKGGCTAVSNANASFEDDDVVIRTLEYAAGLGLTVVFYPEE
EECCCCCCCCCCCCCHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCH
AQIAKDGCVHEGFIASRQGLPMIPAIAETIAIAKYLIMIEATGVRAHFGLISSGKSIELI
HHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHEEEECCCEEEEEECCCCCCEEHH
QAAKAKGLPVTADVAMHQLHLTESLIDGFNSLAHVRPPLRSEQDKEQLRQALKSGVIDAI
HHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHH
CTHHEPLSSSAKMMPFAETLPGISAFDTYVALGIQLVREALFTPLEWVEKVTLIPAQVAN
HCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
MQERWLDEKGWVLVDPDLEWSVDKKSILSSGKNTPLLNQTLTGKVIKTFLI
HHHHHCCCCCCEEECCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SNIKLENVRLLDPVAKIDQVTTIYLKDGKLVESLDQVDETINADGQWLMPTMVDLCARV
CCCEECCEEECCHHHHHCCEEEEEEECCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHH
REPGQQQHGTLKSEGKAARENGILHVITPPDSKPIVQDNGALIHGLIEKAWTDGQFHLHI
HCCCHHHCCCCCCCCCHHHHCCEEEEECCCCCCCEECCCCHHHHHHHHHHCCCCEEEEEE
IGAQTQGLLGRQPANMAALKKGGCTAVSNANASFEDDDVVIRTLEYAAGLGLTVVFYPEE
EECCCCCCCCCCCCCHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCH
AQIAKDGCVHEGFIASRQGLPMIPAIAETIAIAKYLIMIEATGVRAHFGLISSGKSIELI
HHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHEEEECCCEEEEEECCCCCCEEHH
QAAKAKGLPVTADVAMHQLHLTESLIDGFNSLAHVRPPLRSEQDKEQLRQALKSGVIDAI
HHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHH
CTHHEPLSSSAKMMPFAETLPGISAFDTYVALGIQLVREALFTPLEWVEKVTLIPAQVAN
HCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
MQERWLDEKGWVLVDPDLEWSVDKKSILSSGKNTPLLNQTLTGKVIKTFLI
HHHHHCCCCCCEEECCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-dihydroorotate; H2O

Specific reaction: (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]