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The map label for this gene is clpB [H]

Identifier: 50084451

GI number: 50084451

Start: 1262777

End: 1265356

Strand: Direct

Name: clpB [H]

Synonym: ACIAD1265

Alternate gene names: 50084451

Gene position: 1262777-1265356 (Clockwise)

Preceding gene: 50084450

Following gene: 50084452

Centisome position: 35.09

GC content: 42.95

Gene sequence:

>2580_bases
ATGCGTTTTGAAAAATTTACAAATCGGTTGCAACAGGTGCTATCTGATGCACAATCACTTGCAACAGGCAAAGATCATAC
TGCGATTGATGGTCTACATATTCTTGCAACCATGCTCGAAGAGCCATCTAACTTAAGTTTATTGCAACAAGCTGGTGCAC
GTTTACCAGAGTTGAAACAGAAGCTTGAACAAGCGCTCAAAGATGCACCGACTATTACTAATCCGACAGGTGATGTCAAT
CTAAATCCTGAAGCGGTAAAAGTATTAAATCTGGCGGATCGCCATGCTCAAAAGGCAGGGGATGAATTTTTGTCTACAGA
TTGGGTACTGTTGGCATTAACCGAAACAGGTAGTACCAAAAGTTTATTAAATAGCGTAGGTGTAACTACAGATACCTTGC
GCAAAGTCATTGAATCTATTCGAGGCAACGATAAAGTTATGAGTAATAATCACGAAGATCAACGTGACTCATTAAACAAA
TATACCATCGATCTGACTGAGCGAGCTTTGGCAGGTAAACTTGATCCTGTGATTGGGCGTGATGATGAGATTCGCCGTAC
CATTCAGGTACTTTCACGTCGTACCAAGAATAACCCTGTTCTGATAGGTGAGCCTGGGGTTGGTAAAACCGCGATTGTAG
AAGGCTTGGCACAGCGTATTGTGAATGGTGAGGTGCCAGAAAGCTTAAAAAATAAACGCGTATTGTCACTCGATTTAGGT
TCACTACTTGCAGGTGCAAAATATCGCGGTGAGTTTGAAGAGCGTTTAAAAGCAGTACTCAATGATTTGGCCAAACAAGA
TGGTCAGGTAATTTTATTTATTGATGAGTTACATACTTTAGTTGGTGCAGGTAAATCCGATGGTGCCATGGATGCAGGTA
ACATGCTCAAACCTGCTTTGGCACGTGGTGAGTTGCGTTGTGTCGGGGCGACAACTTTAGATGAGTATCGCCAATATATT
GAAAAAGATGCTGCGTTAGAACGTCGTTTCCAGAAAGTATTGGTTGATGAGCCAAGTGTAGAAGATACCATCGCTATTTT
GCGTGGTTTGAAAGAACGTTACGAAGTGCATCATGGTGTAAAAATTCTCGACTCTGCGATTATTGCTGCTGCGAAAATGT
CGCACCGCTATATTACAGATCGTCAGTTGCCAGATAAAGCCATTGATTTGATTGATGAGGCGGCTTCACGTATCAAGATG
GAGCTAGATTCAAAACCAGAAGCACTGGATCGTTTGGAGCGAAGAATCATTCAGCTCAAAATGCAGCTTGAAGCAGTTAA
AAAAGATGAAGATCTAGGAAGTAAAGCCGAGGTTAAACATCTGGAAGAGCAGATTTCTATCGTTCAAAAAGAATATAACG
ATATGGAAGAGGTCTGGCGTGCAGAAAAGGCCTTGGTTGAAGGCACGAAAGACGCACAGGTCAAGCTCGATCAAGCCCGT
ATTGCATTTGAAAAAGCGCAACGTGAAGGTGATTTGGCAGAGGCTGCACGTTTACAATATGGTGTGATTCCTGAGTTACA
AAAGCAGCTTGAGCAAGATGAAGTGGCTGAAGAAAATGAAGAGCCAAAACTATTGCGAAATAAAGTCACAGATAATGAAA
TTGCTGAAGTGGTGAGTGCTGCGACAGGGATTCCAGTGGCTAAAATGTTGCAGGGTGAACGTGAAAAACTTTTACAAATG
GAAAGCTTTTTGCATAACCGTGTAGTTGGACAAGATGAGGCTGTCATTGCGGTTTCGAATGCTGTGCGTCGTTCTCGTGC
GGGCTTGTCGGATCCAAATCGTCCAAGTGGTTCATTCTTATTCCTTGGGCCAACAGGTGTCGGTAAAACTGAATTAACCA
AAGCACTGGCAAACTTCTTGTTTGATAGTGACGATGCCATGATTCGGATTGACATGAGTGAATTTATGGAAAAACACTCG
GTGAGTCGCTTGGTGGGTGCACCTCCAGGTTATGTGGGCTATGAAGAAGGTGGTGTGTTAACCGAAGCGGTACGCCGTAA
ACCATATAGCGTGGTACTATTTGATGAGGTTGAAAAAGCGCATCCCGATGTTTTTAACATTTTACTACAGGTGCTCGATG
ATGGTCGTCTGACTGATTCACAAGGTCGGGTCATTGACTTTAAAAATACTGTGATTGTGATGACCTCAAACCTCGGATCA
CAAGATGTACGTGAGCTGGGTGAAGGTGCGACTGATGATGAGGTACGTGCAGTGGTGATGTCTGCGGTGAGTCAACATTT
CCGTCCAGAGTTTATCAATCGAATTGATGAACTGGTAGTATTCCACTCGTTACAAAAATCGCAAATTCGTGGTATCGCCG
ATATTCAATTGGATCGTTTACGTTCACGTTTGGCCGAGCGTGACATTGGTTTGAATATTGATGATAGTGCCTTTGATATT
CTGATTGATGCTGGTTTCGATCCTGTCTATGGTGCACGTCCTTTGAAACGCGCAATCCAGCAACAGGTTGAAAATACATT
AGCCCAGAAAATTCTGGCAGGTGATTTCCAGCCAGGAGATAACATCATCATCAAAGGTGATCATGGTGACTTGGTGTTTG
ATAAATTAAAACTGAGTTAA

Upstream 100 bases:

>100_bases
TAGTAATAACTTTATGAAAAATATAAAAATTTATTTGAAATTTCAGAAAATGATCCCATGTAATAGTCAAGTAGAAAATT
GATGATGAGGCATAACAATA

Downstream 100 bases:

>100_bases
GACGCAACATTGTTTAAAATCTCAAAAAAGAGAATGTGTACGCATATTCTCTTTTTTTATTATTCATCTTTTCTTGTTTG
CTTCATGAGCAAAACTCATA

Product: ATP-dependent protease, Hsp 100, part of multi-chaperone system with DnaK, DnaJ, and GrpE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 859; Mature: 859

Protein sequence:

>859_residues
MRFEKFTNRLQQVLSDAQSLATGKDHTAIDGLHILATMLEEPSNLSLLQQAGARLPELKQKLEQALKDAPTITNPTGDVN
LNPEAVKVLNLADRHAQKAGDEFLSTDWVLLALTETGSTKSLLNSVGVTTDTLRKVIESIRGNDKVMSNNHEDQRDSLNK
YTIDLTERALAGKLDPVIGRDDEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPESLKNKRVLSLDLG
SLLAGAKYRGEFEERLKAVLNDLAKQDGQVILFIDELHTLVGAGKSDGAMDAGNMLKPALARGELRCVGATTLDEYRQYI
EKDAALERRFQKVLVDEPSVEDTIAILRGLKERYEVHHGVKILDSAIIAAAKMSHRYITDRQLPDKAIDLIDEAASRIKM
ELDSKPEALDRLERRIIQLKMQLEAVKKDEDLGSKAEVKHLEEQISIVQKEYNDMEEVWRAEKALVEGTKDAQVKLDQAR
IAFEKAQREGDLAEAARLQYGVIPELQKQLEQDEVAEENEEPKLLRNKVTDNEIAEVVSAATGIPVAKMLQGEREKLLQM
ESFLHNRVVGQDEAVIAVSNAVRRSRAGLSDPNRPSGSFLFLGPTGVGKTELTKALANFLFDSDDAMIRIDMSEFMEKHS
VSRLVGAPPGYVGYEEGGVLTEAVRRKPYSVVLFDEVEKAHPDVFNILLQVLDDGRLTDSQGRVIDFKNTVIVMTSNLGS
QDVRELGEGATDDEVRAVVMSAVSQHFRPEFINRIDELVVFHSLQKSQIRGIADIQLDRLRSRLAERDIGLNIDDSAFDI
LIDAGFDPVYGARPLKRAIQQQVENTLAQKILAGDFQPGDNIIIKGDHGDLVFDKLKLS

Sequences:

>Translated_859_residues
MRFEKFTNRLQQVLSDAQSLATGKDHTAIDGLHILATMLEEPSNLSLLQQAGARLPELKQKLEQALKDAPTITNPTGDVN
LNPEAVKVLNLADRHAQKAGDEFLSTDWVLLALTETGSTKSLLNSVGVTTDTLRKVIESIRGNDKVMSNNHEDQRDSLNK
YTIDLTERALAGKLDPVIGRDDEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPESLKNKRVLSLDLG
SLLAGAKYRGEFEERLKAVLNDLAKQDGQVILFIDELHTLVGAGKSDGAMDAGNMLKPALARGELRCVGATTLDEYRQYI
EKDAALERRFQKVLVDEPSVEDTIAILRGLKERYEVHHGVKILDSAIIAAAKMSHRYITDRQLPDKAIDLIDEAASRIKM
ELDSKPEALDRLERRIIQLKMQLEAVKKDEDLGSKAEVKHLEEQISIVQKEYNDMEEVWRAEKALVEGTKDAQVKLDQAR
IAFEKAQREGDLAEAARLQYGVIPELQKQLEQDEVAEENEEPKLLRNKVTDNEIAEVVSAATGIPVAKMLQGEREKLLQM
ESFLHNRVVGQDEAVIAVSNAVRRSRAGLSDPNRPSGSFLFLGPTGVGKTELTKALANFLFDSDDAMIRIDMSEFMEKHS
VSRLVGAPPGYVGYEEGGVLTEAVRRKPYSVVLFDEVEKAHPDVFNILLQVLDDGRLTDSQGRVIDFKNTVIVMTSNLGS
QDVRELGEGATDDEVRAVVMSAVSQHFRPEFINRIDELVVFHSLQKSQIRGIADIQLDRLRSRLAERDIGLNIDDSAFDI
LIDAGFDPVYGARPLKRAIQQQVENTLAQKILAGDFQPGDNIIIKGDHGDLVFDKLKLS
>Mature_859_residues
MRFEKFTNRLQQVLSDAQSLATGKDHTAIDGLHILATMLEEPSNLSLLQQAGARLPELKQKLEQALKDAPTITNPTGDVN
LNPEAVKVLNLADRHAQKAGDEFLSTDWVLLALTETGSTKSLLNSVGVTTDTLRKVIESIRGNDKVMSNNHEDQRDSLNK
YTIDLTERALAGKLDPVIGRDDEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPESLKNKRVLSLDLG
SLLAGAKYRGEFEERLKAVLNDLAKQDGQVILFIDELHTLVGAGKSDGAMDAGNMLKPALARGELRCVGATTLDEYRQYI
EKDAALERRFQKVLVDEPSVEDTIAILRGLKERYEVHHGVKILDSAIIAAAKMSHRYITDRQLPDKAIDLIDEAASRIKM
ELDSKPEALDRLERRIIQLKMQLEAVKKDEDLGSKAEVKHLEEQISIVQKEYNDMEEVWRAEKALVEGTKDAQVKLDQAR
IAFEKAQREGDLAEAARLQYGVIPELQKQLEQDEVAEENEEPKLLRNKVTDNEIAEVVSAATGIPVAKMLQGEREKLLQM
ESFLHNRVVGQDEAVIAVSNAVRRSRAGLSDPNRPSGSFLFLGPTGVGKTELTKALANFLFDSDDAMIRIDMSEFMEKHS
VSRLVGAPPGYVGYEEGGVLTEAVRRKPYSVVLFDEVEKAHPDVFNILLQVLDDGRLTDSQGRVIDFKNTVIVMTSNLGS
QDVRELGEGATDDEVRAVVMSAVSQHFRPEFINRIDELVVFHSLQKSQIRGIADIQLDRLRSRLAERDIGLNIDDSAFDI
LIDAGFDPVYGARPLKRAIQQQVENTLAQKILAGDFQPGDNIIIKGDHGDLVFDKLKLS

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=309, Percent_Identity=38.1877022653722, Blast_Score=209, Evalue=7e-54,
Organism=Escherichia coli, GI1788943, Length=845, Percent_Identity=66.2721893491124, Blast_Score=1118, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=390, Percent_Identity=42.5641025641026, Blast_Score=290, Evalue=3e-79,
Organism=Saccharomyces cerevisiae, GI6320464, Length=701, Percent_Identity=55.206847360913, Blast_Score=786, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=860, Percent_Identity=43.6046511627907, Blast_Score=696, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 95351; Mature: 95351

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFEKFTNRLQQVLSDAQSLATGKDHTAIDGLHILATMLEEPSNLSLLQQAGARLPELKQ
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHH
KLEQALKDAPTITNPTGDVNLNPEAVKVLNLADRHAQKAGDEFLSTDWVLLALTETGSTK
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHH
SLLNSVGVTTDTLRKVIESIRGNDKVMSNNHEDQRDSLNKYTIDLTERALAGKLDPVIGR
HHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHEEEHHHHHHHHCCCCCCCCC
DDEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPESLKNKRVLSLDLG
CHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHCCEEEEEEHH
SLLAGAKYRGEFEERLKAVLNDLAKQDGQVILFIDELHTLVGAGKSDGAMDAGNMLKPAL
HHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHHHHH
ARGELRCVGATTLDEYRQYIEKDAALERRFQKVLVDEPSVEDTIAILRGLKERYEVHHGV
HCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
KILDSAIIAAAKMSHRYITDRQLPDKAIDLIDEAASRIKMELDSKPEALDRLERRIIQLK
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
MQLEAVKKDEDLGSKAEVKHLEEQISIVQKEYNDMEEVWRAEKALVEGTKDAQVKLDQAR
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEHHHHH
IAFEKAQREGDLAEAARLQYGVIPELQKQLEQDEVAEENEEPKLLRNKVTDNEIAEVVSA
HHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHH
ATGIPVAKMLQGEREKLLQMESFLHNRVVGQDEAVIAVSNAVRRSRAGLSDPNRPSGSFL
HCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCEE
FLGPTGVGKTELTKALANFLFDSDDAMIRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGVL
EECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
TEAVRRKPYSVVLFDEVEKAHPDVFNILLQVLDDGRLTDSQGRVIDFKNTVIVMTSNLGS
HHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCCCH
QDVRELGEGATDDEVRAVVMSAVSQHFRPEFINRIDELVVFHSLQKSQIRGIADIQLDRL
HHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RSRLAERDIGLNIDDSAFDILIDAGFDPVYGARPLKRAIQQQVENTLAQKILAGDFQPGD
HHHHHHHCCCCCCCCCHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NIIIKGDHGDLVFDKLKLS
CEEEECCCCCEEHHHHCCC
>Mature Secondary Structure
MRFEKFTNRLQQVLSDAQSLATGKDHTAIDGLHILATMLEEPSNLSLLQQAGARLPELKQ
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHH
KLEQALKDAPTITNPTGDVNLNPEAVKVLNLADRHAQKAGDEFLSTDWVLLALTETGSTK
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHH
SLLNSVGVTTDTLRKVIESIRGNDKVMSNNHEDQRDSLNKYTIDLTERALAGKLDPVIGR
HHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHEEEHHHHHHHHCCCCCCCCC
DDEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPESLKNKRVLSLDLG
CHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHCCEEEEEEHH
SLLAGAKYRGEFEERLKAVLNDLAKQDGQVILFIDELHTLVGAGKSDGAMDAGNMLKPAL
HHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHHHHH
ARGELRCVGATTLDEYRQYIEKDAALERRFQKVLVDEPSVEDTIAILRGLKERYEVHHGV
HCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
KILDSAIIAAAKMSHRYITDRQLPDKAIDLIDEAASRIKMELDSKPEALDRLERRIIQLK
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
MQLEAVKKDEDLGSKAEVKHLEEQISIVQKEYNDMEEVWRAEKALVEGTKDAQVKLDQAR
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEHHHHH
IAFEKAQREGDLAEAARLQYGVIPELQKQLEQDEVAEENEEPKLLRNKVTDNEIAEVVSA
HHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHH
ATGIPVAKMLQGEREKLLQMESFLHNRVVGQDEAVIAVSNAVRRSRAGLSDPNRPSGSFL
HCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCEE
FLGPTGVGKTELTKALANFLFDSDDAMIRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGVL
EECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
TEAVRRKPYSVVLFDEVEKAHPDVFNILLQVLDDGRLTDSQGRVIDFKNTVIVMTSNLGS
HHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCCCH
QDVRELGEGATDDEVRAVVMSAVSQHFRPEFINRIDELVVFHSLQKSQIRGIADIQLDRL
HHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RSRLAERDIGLNIDDSAFDILIDAGFDPVYGARPLKRAIQQQVENTLAQKILAGDFQPGD
HHHHHHHCCCCCCCCCHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NIIIKGDHGDLVFDKLKLS
CEEEECCCCCEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]