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The map label for this gene is lysC [H]

Identifier: 50084438

GI number: 50084438

Start: 1249850

End: 1251130

Strand: Reverse

Name: lysC [H]

Synonym: ACIAD1252

Alternate gene names: 50084438

Gene position: 1251130-1249850 (Counterclockwise)

Preceding gene: 50084439

Following gene: 50084437

Centisome position: 34.77

GC content: 43.01

Gene sequence:

>1281_bases
ATGGCATTAATCGTTCAAAAATATGGCGGTACTTCTATGGGTACTCCCGAGCGCATTTTAAATGTTGCTCGTCGTGTGAA
GCGCTGGCATGATCACGGCCACAAAGTGGTTGTGGTTGTATCAGCGATGAGTGGCGAAACCAATCGCCTACTTGCTCTCG
CGAAAGCCATTACCGATACCCCTGATCCGCGTGAACTCGACCAGATGGTCTCTACAGGCGAACAGGTGACGATTTCAATG
TTAGCAATGGCACTCAATTCGATTGGTGTAGAAGCTAAATCATATACAGGTCGTCAAGTTGGTATTAAAACAGACAGCGC
ATATAACAAAGCACGTATTGAAGCGATTGATACCGATGTCATGACCAAAGATCTGGATGCTGGGCGTGTACTGGTTGTTG
CAGGCTTTCAAGGTGTAGATGAAGAAGGTAATACAACCACCCTAGGTCGTGGTGGATCAGACACTACAGGTGTTGCACTT
GCAGCAGCGCTCAAAGCAGATGAATGCCAGATCTACACTGACGTTGATGGCGTATATACTACCGATCCACGCGTTGCACC
TAAAGCCAAAAAAGTTGATCGTATTTCATTTGAAGAAATGTTAGAAATGGCTTCTTTAGGTTCTAAAGTTCTTCAAATTC
GCTCTGTTGAGTTTGCTGGCAAATATCAAGTGCCACTGCGCGTATTATCAAGCTTTGATAATGACAATGATGGTGCATTT
GATGAAGAATTTAAACAAAACGTAGGTACGTTAATTACAACTGAGGCGGAAGATACAATGGAACAACCGATTATCTCTGG
TATCGCATTTAATCGTGACGAAGCAAAATTAACCATCCTGGGTGTTCCAGATGAGCCAGGTATTGCCGCTAAAATTTTGG
TTCCAATTAGTGCTGCCAACATCGAAGTTGACATGATCGTACAAAATGTCGAAGAAGATGGAACAACGGATTTTACATTT
ACGGTAAACCGTGGCGACTTGGCAAAAGCAAAGAAAATCCTTGAAGAAACTGCTTCAAATATCGGTGCTCGTGAAGTTTC
AACACGTGATGATATTGTCAAAGTTTCAATTGTGGGTGTAGGTATGCGCTCACATGCAGGTGTTGCGAGTAAAATGTTTA
TGGCTTTGGCCGAAGAAAGCATCAACATTTTGATGATTTCGACTTCTGAAATTAAGGTTTCAGTACTCATCGAAGAAAAT
TATCTAGAGCTTGCTGTTCGTACACTCCATACTGCGTTTGGTTTGGATCGTGAACATGGCGAAAGCAGTGCACGTGCTTA
A

Upstream 100 bases:

>100_bases
TTGCAGCACTACCTGCATGGCTTGAACAGCACTAAGACTTCACTTTTGTGTAACTGACCCTGAATAAGCCTCTCAGGGTC
ATGGCTTATATGGAACAACT

Downstream 100 bases:

>100_bases
GGATTGATCAATCAACCTTGACTTAGTTTACTTTTCAAAGAATCGTGACAAAAAAACAGAGCCACTATAGTGTTTTTTAT
AGTGGCTCTGTTATATTAGC

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MALIVQKYGGTSMGTPERILNVARRVKRWHDHGHKVVVVVSAMSGETNRLLALAKAITDTPDPRELDQMVSTGEQVTISM
LAMALNSIGVEAKSYTGRQVGIKTDSAYNKARIEAIDTDVMTKDLDAGRVLVVAGFQGVDEEGNTTTLGRGGSDTTGVAL
AAALKADECQIYTDVDGVYTTDPRVAPKAKKVDRISFEEMLEMASLGSKVLQIRSVEFAGKYQVPLRVLSSFDNDNDGAF
DEEFKQNVGTLITTEAEDTMEQPIISGIAFNRDEAKLTILGVPDEPGIAAKILVPISAANIEVDMIVQNVEEDGTTDFTF
TVNRGDLAKAKKILEETASNIGAREVSTRDDIVKVSIVGVGMRSHAGVASKMFMALAEESINILMISTSEIKVSVLIEEN
YLELAVRTLHTAFGLDREHGESSARA

Sequences:

>Translated_426_residues
MALIVQKYGGTSMGTPERILNVARRVKRWHDHGHKVVVVVSAMSGETNRLLALAKAITDTPDPRELDQMVSTGEQVTISM
LAMALNSIGVEAKSYTGRQVGIKTDSAYNKARIEAIDTDVMTKDLDAGRVLVVAGFQGVDEEGNTTTLGRGGSDTTGVAL
AAALKADECQIYTDVDGVYTTDPRVAPKAKKVDRISFEEMLEMASLGSKVLQIRSVEFAGKYQVPLRVLSSFDNDNDGAF
DEEFKQNVGTLITTEAEDTMEQPIISGIAFNRDEAKLTILGVPDEPGIAAKILVPISAANIEVDMIVQNVEEDGTTDFTF
TVNRGDLAKAKKILEETASNIGAREVSTRDDIVKVSIVGVGMRSHAGVASKMFMALAEESINILMISTSEIKVSVLIEEN
YLELAVRTLHTAFGLDREHGESSARA
>Mature_425_residues
ALIVQKYGGTSMGTPERILNVARRVKRWHDHGHKVVVVVSAMSGETNRLLALAKAITDTPDPRELDQMVSTGEQVTISML
AMALNSIGVEAKSYTGRQVGIKTDSAYNKARIEAIDTDVMTKDLDAGRVLVVAGFQGVDEEGNTTTLGRGGSDTTGVALA
AALKADECQIYTDVDGVYTTDPRVAPKAKKVDRISFEEMLEMASLGSKVLQIRSVEFAGKYQVPLRVLSSFDNDNDGAFD
EEFKQNVGTLITTEAEDTMEQPIISGIAFNRDEAKLTILGVPDEPGIAAKILVPISAANIEVDMIVQNVEEDGTTDFTFT
VNRGDLAKAKKILEETASNIGAREVSTRDDIVKVSIVGVGMRSHAGVASKMFMALAEESINILMISTSEIKVSVLIEENY
LELAVRTLHTAFGLDREHGESSARA

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=358, Percent_Identity=31.0055865921788, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1790455, Length=377, Percent_Identity=27.8514588859416, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1790376, Length=387, Percent_Identity=21.9638242894057, Blast_Score=72, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320893, Length=501, Percent_Identity=23.75249500998, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 45959; Mature: 45828

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIVQKYGGTSMGTPERILNVARRVKRWHDHGHKVVVVVSAMSGETNRLLALAKAITDT
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHCCC
PDPRELDQMVSTGEQVTISMLAMALNSIGVEAKSYTGRQVGIKTDSAYNKARIEAIDTDV
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCEEEEEECCHH
MTKDLDAGRVLVVAGFQGVDEEGNTTTLGRGGSDTTGVALAAALKADECQIYTDVDGVYT
HHCCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCEEE
TDPRVAPKAKKVDRISFEEMLEMASLGSKVLQIRSVEFAGKYQVPLRVLSSFDNDNDGAF
CCCCCCCCCHHHHCCCHHHHHHHHHHCHHHEEEEEEEECCCCCHHHHHHHCCCCCCCCCC
DEEFKQNVGTLITTEAEDTMEQPIISGIAFNRDEAKLTILGVPDEPGIAAKILVPISAAN
HHHHHHCCCCEEEECCHHHHHHHHHHCEEECCCCCEEEEEECCCCCCCEEEEEEEEEECC
IEVDMIVQNVEEDGTTDFTFTVNRGDLAKAKKILEETASNIGAREVSTRDDIVKVSIVGV
EEEEEEEEHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEE
GMRSHAGVASKMFMALAEESINILMISTSEIKVSVLIEENYLELAVRTLHTAFGLDREHG
CCCCCCCHHHHHHHHHHHCCCEEEEEEECCEEEEEEEECCHHHHHHHHHHHHHCCCHHCC
ESSARA
CCCCCC
>Mature Secondary Structure 
ALIVQKYGGTSMGTPERILNVARRVKRWHDHGHKVVVVVSAMSGETNRLLALAKAITDT
EEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHCCC
PDPRELDQMVSTGEQVTISMLAMALNSIGVEAKSYTGRQVGIKTDSAYNKARIEAIDTDV
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCEEEEEECCHH
MTKDLDAGRVLVVAGFQGVDEEGNTTTLGRGGSDTTGVALAAALKADECQIYTDVDGVYT
HHCCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCEEE
TDPRVAPKAKKVDRISFEEMLEMASLGSKVLQIRSVEFAGKYQVPLRVLSSFDNDNDGAF
CCCCCCCCCHHHHCCCHHHHHHHHHHCHHHEEEEEEEECCCCCHHHHHHHCCCCCCCCCC
DEEFKQNVGTLITTEAEDTMEQPIISGIAFNRDEAKLTILGVPDEPGIAAKILVPISAAN
HHHHHHCCCCEEEECCHHHHHHHHHHCEEECCCCCEEEEEECCCCCCCEEEEEEEEEECC
IEVDMIVQNVEEDGTTDFTFTVNRGDLAKAKKILEETASNIGAREVSTRDDIVKVSIVGV
EEEEEEEEHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEE
GMRSHAGVASKMFMALAEESINILMISTSEIKVSVLIEENYLELAVRTLHTAFGLDREHG
CCCCCCCHHHHHHHHHHHCCCEEEEEEECCEEEEEEEECCHHHHHHHHHHHHHCCCHHCC
ESSARA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]