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The map label for this gene is purB [H]

Identifier: 50084407

GI number: 50084407

Start: 1222005

End: 1223393

Strand: Reverse

Name: purB [H]

Synonym: ACIAD1219

Alternate gene names: 50084407

Gene position: 1223393-1222005 (Counterclockwise)

Preceding gene: 50084408

Following gene: 50084406

Centisome position: 34.0

GC content: 40.75

Gene sequence:

>1389_bases
ATGAACGCTTTAACTGCCCTCTCTCCATTAGATGGACGTTATGCCAGCAAATGTGATGCTTTACGCCCTTTTCTTTCTGA
GTTTGGTTTAATCCATGCTCGTGTGACTGTTGAAGTACGCTGGTTACAAGCGCTCTCTAACCATGCAGGGATCATTGAAG
TCGCTCCTTTTTCTACAGAAACCAATGCTGCTTTAGATTCAATTGTGGCAAATTTTTCTGAAGATGATGCAAATCGTATT
AAAGAAATCGAACGCACCACCAATCATGATGTAAAAGCCGTTGAGTATTTTCTTAAAGAGAAAATCGCAGGTCTGGATGA
ATTAAAAAATGCAGGTGAATTCATTCACTTTGCATGTACCTCTGAAGACATCAATAACTTGTCTCATGCTTTGATGCTTA
AAAATGGTCGTGAAGTTCTGGTTTCAAGCATGAAACAGATTCTAAATGCAATTGCAGCCTTAGCCACTACACATGCTGAA
CAACCAATGTTGTCACGTACACATGGTCAAACTGCAAGCCCGACAACACTGGGCAAAGAAATGGCAAACGTCGCCTATCG
TTTGGCACGTCAGATTAAACAATTTGAAAATGTCGAATTGCTTGGCAAAATCAATGGTGCTGTCGGTAACTACAATGCGC
ACCTTTCTGCTTATCCAGATATTGATTGGGCTGCGCATTCACAAGCATTTGTGGAATCTTTAGGTTTAACCTTTAACCCA
TATACCACTCAAATTGAACCACACGACTATATGGCGGAATTGTTCGATGCATTGCGTCGTTACAACACAATTCTGATCGA
TTTTAACCGCGATGTATGGGGTTATATTTCTTTAGGTTACTTCAAACAAAAACTGAAAGATGGTGAAGTGGGTTCTTCAA
CGATGCCACATAAAGTTAACCCGATTGATTTTGAAAACTCTGAAGGTAACTTAGGCATTGCAAATGCTGTATTAGGCCAT
TTGGGTGAAAAACTTCCAGTTTCTCGCTGGCAGCGCGATTTAACAGACTCGACTGTACTGCGTAATATGGGTGTTGGCTT
TGCACAAAGCCTGATTGCTTTTGATGCTTGCCTAAAAGGAATTGGTAAACTAGAGCTCAATGCAGCTCGTCTTGCAGAAG
ATCTCAATCAAGCACAAGAAGTCTTGGCTGAACCAATTCAAACTGTTATGCGTCGTTACAATGTTGAAAAACCTTATGAA
AAGTTAAAAGCATTAACACGTGGTCAAGCAATGACGCGCGACATGATGGTTAACTTTGTAAACGGTGATGAACTTAGTCA
GGTACCTGCCGAAGAACGTGCACGCTTAGCAGAAATGACACCAGCCAGCTATACAGGTAACGCTGCAGATCAAGCAAAAC
AGATCAATGATTTGATCAATAAAATTTAA

Upstream 100 bases:

>100_bases
CAAGATATTCAAACTCTCGCTCGCTTACAGTATCAGATGGTTTAAATCTGATGCTGCAGCGCTTTTCGTTTTACATCAAT
TCCCTGTTTAAGGAATCTCT

Downstream 100 bases:

>100_bases
TCTGAATCAGTTAAACTAGAGCGGAATCATTTTAAATGGTTCCGCTTTTTTATACTCATTTCATACATAACGAAGAATCT
CAATGATTGAGAAAAAATTT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MNALTALSPLDGRYASKCDALRPFLSEFGLIHARVTVEVRWLQALSNHAGIIEVAPFSTETNAALDSIVANFSEDDANRI
KEIERTTNHDVKAVEYFLKEKIAGLDELKNAGEFIHFACTSEDINNLSHALMLKNGREVLVSSMKQILNAIAALATTHAE
QPMLSRTHGQTASPTTLGKEMANVAYRLARQIKQFENVELLGKINGAVGNYNAHLSAYPDIDWAAHSQAFVESLGLTFNP
YTTQIEPHDYMAELFDALRRYNTILIDFNRDVWGYISLGYFKQKLKDGEVGSSTMPHKVNPIDFENSEGNLGIANAVLGH
LGEKLPVSRWQRDLTDSTVLRNMGVGFAQSLIAFDACLKGIGKLELNAARLAEDLNQAQEVLAEPIQTVMRRYNVEKPYE
KLKALTRGQAMTRDMMVNFVNGDELSQVPAEERARLAEMTPASYTGNAADQAKQINDLINKI

Sequences:

>Translated_462_residues
MNALTALSPLDGRYASKCDALRPFLSEFGLIHARVTVEVRWLQALSNHAGIIEVAPFSTETNAALDSIVANFSEDDANRI
KEIERTTNHDVKAVEYFLKEKIAGLDELKNAGEFIHFACTSEDINNLSHALMLKNGREVLVSSMKQILNAIAALATTHAE
QPMLSRTHGQTASPTTLGKEMANVAYRLARQIKQFENVELLGKINGAVGNYNAHLSAYPDIDWAAHSQAFVESLGLTFNP
YTTQIEPHDYMAELFDALRRYNTILIDFNRDVWGYISLGYFKQKLKDGEVGSSTMPHKVNPIDFENSEGNLGIANAVLGH
LGEKLPVSRWQRDLTDSTVLRNMGVGFAQSLIAFDACLKGIGKLELNAARLAEDLNQAQEVLAEPIQTVMRRYNVEKPYE
KLKALTRGQAMTRDMMVNFVNGDELSQVPAEERARLAEMTPASYTGNAADQAKQINDLINKI
>Mature_462_residues
MNALTALSPLDGRYASKCDALRPFLSEFGLIHARVTVEVRWLQALSNHAGIIEVAPFSTETNAALDSIVANFSEDDANRI
KEIERTTNHDVKAVEYFLKEKIAGLDELKNAGEFIHFACTSEDINNLSHALMLKNGREVLVSSMKQILNAIAALATTHAE
QPMLSRTHGQTASPTTLGKEMANVAYRLARQIKQFENVELLGKINGAVGNYNAHLSAYPDIDWAAHSQAFVESLGLTFNP
YTTQIEPHDYMAELFDALRRYNTILIDFNRDVWGYISLGYFKQKLKDGEVGSSTMPHKVNPIDFENSEGNLGIANAVLGH
LGEKLPVSRWQRDLTDSTVLRNMGVGFAQSLIAFDACLKGIGKLELNAARLAEDLNQAQEVLAEPIQTVMRRYNVEKPYE
KLKALTRGQAMTRDMMVNFVNGDELSQVPAEERARLAEMTPASYTGNAADQAKQINDLINKI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=449, Percent_Identity=65.0334075723831, Blast_Score=614, Evalue=1e-177,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51231; Mature: 51231

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALTALSPLDGRYASKCDALRPFLSEFGLIHARVTVEVRWLQALSNHAGIIEVAPFSTE
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHCCCCEEEECCCCCC
TNAALDSIVANFSEDDANRIKEIERTTNHDVKAVEYFLKEKIAGLDELKNAGEFIHFACT
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHCCCCEEEEEEC
SEDINNLSHALMLKNGREVLVSSMKQILNAIAALATTHAEQPMLSRTHGQTASPTTLGKE
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHH
MANVAYRLARQIKQFENVELLGKINGAVGNYNAHLSAYPDIDWAAHSQAFVESLGLTFNP
HHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCC
YTTQIEPHDYMAELFDALRRYNTILIDFNRDVWGYISLGYFKQKLKDGEVGSSTMPHKVN
CCCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PIDFENSEGNLGIANAVLGHLGEKLPVSRWQRDLTDSTVLRNMGVGFAQSLIAFDACLKG
CCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
IGKLELNAARLAEDLNQAQEVLAEPIQTVMRRYNVEKPYEKLKALTRGQAMTRDMMVNFV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
NGDELSQVPAEERARLAEMTPASYTGNAADQAKQINDLINKI
CCCHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNALTALSPLDGRYASKCDALRPFLSEFGLIHARVTVEVRWLQALSNHAGIIEVAPFSTE
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHCCCCEEEECCCCCC
TNAALDSIVANFSEDDANRIKEIERTTNHDVKAVEYFLKEKIAGLDELKNAGEFIHFACT
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHCCCCEEEEEEC
SEDINNLSHALMLKNGREVLVSSMKQILNAIAALATTHAEQPMLSRTHGQTASPTTLGKE
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHH
MANVAYRLARQIKQFENVELLGKINGAVGNYNAHLSAYPDIDWAAHSQAFVESLGLTFNP
HHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCC
YTTQIEPHDYMAELFDALRRYNTILIDFNRDVWGYISLGYFKQKLKDGEVGSSTMPHKVN
CCCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PIDFENSEGNLGIANAVLGHLGEKLPVSRWQRDLTDSTVLRNMGVGFAQSLIAFDACLKG
CCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
IGKLELNAARLAEDLNQAQEVLAEPIQTVMRRYNVEKPYEKLKALTRGQAMTRDMMVNFV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
NGDELSQVPAEERARLAEMTPASYTGNAADQAKQINDLINKI
CCCHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]