Click here to switch to the map view.

The map label for this gene is ubiH [H]

Identifier: 50084321

GI number: 50084321

Start: 1114844

End: 1116052

Strand: Direct

Name: ubiH [H]

Synonym: ACIAD1127

Alternate gene names: 50084321

Gene position: 1114844-1116052 (Clockwise)

Preceding gene: 50084320

Following gene: 50084322

Centisome position: 30.98

GC content: 44.0

Gene sequence:

>1209_bases
GTGCAACAGCAAGTCATTATTGTGGGTGGGGGAATGGTCGGACTGAGTCTGGCTTTAATGTTGGCGAAGCAAAAAATCGC
GGTCAAACTTTTGGAAGCAATACGTTATCCAGATTACGACGATGCGAATCTGGCGCCTTATCACTCCAGTTTTGATGCAC
GTAATACTGCGCTTTCACGCCGTAGTGTACAAATTTATCAAAAATTAGAGTTGTGGAATGCACTTCAGCAGCATGCTACC
CCAATTTTGCAGGTTCATATTACAGAGCAGGGAAGTTTTGGTAAGGCACGCTTGACTGCCGAACAAGAGAAAGTCGAAAG
TTTTGGGCAAGTCATTGAAAATGCCTGGCTTGGTCGGGTGTTGCTTACCGAGGTTCGCGAGCAAACCTTGATTGAGCTGA
TTGATGGGATTGAAGTGACGGCATTGCATCAAGACGCTGAAAAGGTCTTTATTGAGGCCAAACGTGGTGATGAACAGCTT
AGTTTAAGCAGTCAGTTAGTAATTGCTGCAGATGGCCGTGATTCCTTTTGTCGTCAAGCCTTAGGTATTGGTGCCGATGT
GCATGATTATGATCAAGTTGCAATTGTCACCGCAGTACAGACATCTAAACCACATCAACATATTGGTTTTGAACGTTTTA
GCGAGTTGGGGCCTTTAGCATTACTCCCATTGCCAGGAGAGTATCGTCGATCTGTGGTATGGCCTGTGAAAAAAGGTACA
GAAAGTGAATGGCTGGGTGAAGATAACGATCAGCATTTTTTGAATGCCTTGCAACAGACTTATGGCGATCGTGCAGGGAA
ATTTGAAAAAACGGGTAAACGTTTTAGTTATCCATTGTCTCAGGTACTCGCGCACAAACAGGCGGTGGGTCGGGTGGTAT
TGATGGGAAATGCAGCACATACCATTCATCCTGTTGCAGGGCAGGGTTTTAATTTATGTTTGCGTGATGCAGATGTTCTG
GTTCGTTATTTATTACAACAGTTAGCGACTTCGAATGATATCGGTCAACCAGAAAACCTGTTGGCGTATGAGCAGGCGCG
TTTAAAAGATCAGCAGCGTGTAATTCGGTTTTGTGATTCTGTTGTCCGTGGTTTTAGTCATCAAAATCCTATTCTTAAGT
TATTGCGTAATACTGGTTTGATTGCTTTTGATGTGATTCCTGGAATCAAGCCGTTGGTTGCCAATTACGCTATGGGATTA
AAAGCATGA

Upstream 100 bases:

>100_bases
CCATTGGTTCTGACCAAAGATGTGGTCAAGGAAATCGCCGATATTGAACAGCTCATGGCTCAAGCAAAGTCATAACGATT
AGAAAACGGGAGTAGATCAC

Downstream 100 bases:

>100_bases
CACAAATCGCAGATGTGGTAATTGTAGGTGGTGGATTGGTTGGTGGCCTAACGGCATTGCTGCTTGCCCAAGGTGGTGTT
CAAGCGACTGTACTGGATGC

Product: 2-octaprenyl-6-methoxyphynol hydroxylase

Products: H2O; 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MQQQVIIVGGGMVGLSLALMLAKQKIAVKLLEAIRYPDYDDANLAPYHSSFDARNTALSRRSVQIYQKLELWNALQQHAT
PILQVHITEQGSFGKARLTAEQEKVESFGQVIENAWLGRVLLTEVREQTLIELIDGIEVTALHQDAEKVFIEAKRGDEQL
SLSSQLVIAADGRDSFCRQALGIGADVHDYDQVAIVTAVQTSKPHQHIGFERFSELGPLALLPLPGEYRRSVVWPVKKGT
ESEWLGEDNDQHFLNALQQTYGDRAGKFEKTGKRFSYPLSQVLAHKQAVGRVVLMGNAAHTIHPVAGQGFNLCLRDADVL
VRYLLQQLATSNDIGQPENLLAYEQARLKDQQRVIRFCDSVVRGFSHQNPILKLLRNTGLIAFDVIPGIKPLVANYAMGL
KA

Sequences:

>Translated_402_residues
MQQQVIIVGGGMVGLSLALMLAKQKIAVKLLEAIRYPDYDDANLAPYHSSFDARNTALSRRSVQIYQKLELWNALQQHAT
PILQVHITEQGSFGKARLTAEQEKVESFGQVIENAWLGRVLLTEVREQTLIELIDGIEVTALHQDAEKVFIEAKRGDEQL
SLSSQLVIAADGRDSFCRQALGIGADVHDYDQVAIVTAVQTSKPHQHIGFERFSELGPLALLPLPGEYRRSVVWPVKKGT
ESEWLGEDNDQHFLNALQQTYGDRAGKFEKTGKRFSYPLSQVLAHKQAVGRVVLMGNAAHTIHPVAGQGFNLCLRDADVL
VRYLLQQLATSNDIGQPENLLAYEQARLKDQQRVIRFCDSVVRGFSHQNPILKLLRNTGLIAFDVIPGIKPLVANYAMGL
KA
>Mature_402_residues
MQQQVIIVGGGMVGLSLALMLAKQKIAVKLLEAIRYPDYDDANLAPYHSSFDARNTALSRRSVQIYQKLELWNALQQHAT
PILQVHITEQGSFGKARLTAEQEKVESFGQVIENAWLGRVLLTEVREQTLIELIDGIEVTALHQDAEKVFIEAKRGDEQL
SLSSQLVIAADGRDSFCRQALGIGADVHDYDQVAIVTAVQTSKPHQHIGFERFSELGPLALLPLPGEYRRSVVWPVKKGT
ESEWLGEDNDQHFLNALQQTYGDRAGKFEKTGKRFSYPLSQVLAHKQAVGRVVLMGNAAHTIHPVAGQGFNLCLRDADVL
VRYLLQQLATSNDIGQPENLLAYEQARLKDQQRVIRFCDSVVRGFSHQNPILKLLRNTGLIAFDVIPGIKPLVANYAMGL
KA

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=271, Percent_Identity=27.6752767527675, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI32967307, Length=271, Percent_Identity=27.6752767527675, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1789274, Length=404, Percent_Identity=33.6633663366337, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1789273, Length=398, Percent_Identity=31.1557788944724, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1786883, Length=406, Percent_Identity=30.0492610837438, Blast_Score=137, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI25146631, Length=436, Percent_Identity=27.7522935779816, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19920760, Length=435, Percent_Identity=24.1379310344828, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 44706; Mature: 44706

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQQVIIVGGGMVGLSLALMLAKQKIAVKLLEAIRYPDYDDANLAPYHSSFDARNTALSR
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
RSVQIYQKLELWNALQQHATPILQVHITEQGSFGKARLTAEQEKVESFGQVIENAWLGRV
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHH
LLTEVREQTLIELIDGIEVTALHQDAEKVFIEAKRGDEQLSLSSQLVIAADGRDSFCRQA
HHHHHHHHHHHHHHCCCEEEEEHHCHHHHEEECCCCCCCCCCCCCEEEEECCCHHHHHHH
LGIGADVHDYDQVAIVTAVQTSKPHQHIGFERFSELGPLALLPLPGEYRRSVVWPVKKGT
HCCCCCCCCCCCEEEEEEEECCCCHHHCCHHHHHHCCCEEEECCCCHHHCCEEEECCCCC
ESEWLGEDNDQHFLNALQQTYGDRAGKFEKTGKRFSYPLSQVLAHKQAVGRVVLMGNAAH
CCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCHHCCCHHHHHHHHHHHCEEEEECCCCC
TIHPVAGQGFNLCLRDADVLVRYLLQQLATSNDIGQPENLLAYEQARLKDQQRVIRFCDS
EECCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VVRGFSHQNPILKLLRNTGLIAFDVIPGIKPLVANYAMGLKA
HHHCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MQQQVIIVGGGMVGLSLALMLAKQKIAVKLLEAIRYPDYDDANLAPYHSSFDARNTALSR
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
RSVQIYQKLELWNALQQHATPILQVHITEQGSFGKARLTAEQEKVESFGQVIENAWLGRV
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHH
LLTEVREQTLIELIDGIEVTALHQDAEKVFIEAKRGDEQLSLSSQLVIAADGRDSFCRQA
HHHHHHHHHHHHHHCCCEEEEEHHCHHHHEEECCCCCCCCCCCCCEEEEECCCHHHHHHH
LGIGADVHDYDQVAIVTAVQTSKPHQHIGFERFSELGPLALLPLPGEYRRSVVWPVKKGT
HCCCCCCCCCCCEEEEEEEECCCCHHHCCHHHHHHCCCEEEECCCCHHHCCEEEECCCCC
ESEWLGEDNDQHFLNALQQTYGDRAGKFEKTGKRFSYPLSQVLAHKQAVGRVVLMGNAAH
CCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCHHCCCHHHHHHHHHHHCEEEEECCCCC
TIHPVAGQGFNLCLRDADVLVRYLLQQLATSNDIGQPENLLAYEQARLKDQQRVIRFCDS
EECCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VVRGFSHQNPILKLLRNTGLIAFDVIPGIKPLVANYAMGLKA
HHHCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-octaprenyl-6-methoxyphenol; O2 [C]

Specific reaction: 2-octaprenyl-6-methoxyphenol + O2 = H2O + 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]