Click here to switch to the map view.

The map label for this gene is rnfB [H]

Identifier: 50084303

GI number: 50084303

Start: 1096552

End: 1097304

Strand: Reverse

Name: rnfB [H]

Synonym: ACIAD1109

Alternate gene names: 50084303

Gene position: 1097304-1096552 (Counterclockwise)

Preceding gene: 50084305

Following gene: 50084302

Centisome position: 30.49

GC content: 44.62

Gene sequence:

>753_bases
TTGCCTCAAACTCAATGTGGTTTGTGTGGGCATCGTGATGGCTGTCTACCTTATGCAAAATCGATGATAGAAGGTGAAGC
TGCAAATAAATGTATTCCTGGAGGTCAGCCTGTTGCAGATGCTTTAGCACAGCTTCTTAATCGTCCTCATCTAAAAGCAG
AACAAAGTATATGGCCGATTCAGTCTGACGGACGACCACAGCGGATGAAAGCAATCATCCGTGAAGATGAATGTATCGGA
TGTACCAAATGTATAAGTGCTTGCCCAGTCGACGCGATTATTGGTAGCGGTAAATTGATGCATACTGTTTTGACGGACTT
GTGTACAGGGTGTGAACTTTGTATTCCACCCTGTCCTGTCGATTGTATTGATCTGGTGGAAGACCGCGAACCCTTGCCAA
CGATAGCTGAGCGAAATAATGAACAGCAACAACTTCGCCAACGCTACTATGCACATATACAACGCGAAGAACAACGCAGC
ATCCATCGCAAAGGCCCTATCGTTCGTGCCGAAATTGATACCGATCTGTTTAGCCAATTTTCACATTGGTTAGAAGAAGT
ACCATCGATTGCGCGCGTCGAGCAAGCTGTAAAAACCGTTGCTTCTGTCGACGCAAAAACAACGATTGAGCTTGCCAAAC
TGCGCACCCAAATCAAAAAACTGGAAAAACAACTCAACATTAGAGCTGATCAACAAAAGCAAACGCAACTTGAACAGTTA
CAACTACGCTTAAATCAGATTCTGGAGGTCTAG

Upstream 100 bases:

>100_bases
AAATACGCTCCTCACGCTGTAATCACCTTTGTTCTAATTGCTTTAACACTTTATGATGAATACCTCTTCTTTTGCTCTTC
TGCGCGAAATTGATAGCATA

Downstream 100 bases:

>100_bases
ATGGCGGTAAAAAATATGACCAAAAAACAGATTCGGACGTTTTTTGAACGCCTTAGAGCGCAGAGACCTTATCCCCAAAC
TGAGCTAAAATATTCAAGCC

Product: iron-sulfur protein

Products: NA

Alternate protein names: Nitrogen fixation protein rnfB [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MPQTQCGLCGHRDGCLPYAKSMIEGEAANKCIPGGQPVADALAQLLNRPHLKAEQSIWPIQSDGRPQRMKAIIREDECIG
CTKCISACPVDAIIGSGKLMHTVLTDLCTGCELCIPPCPVDCIDLVEDREPLPTIAERNNEQQQLRQRYYAHIQREEQRS
IHRKGPIVRAEIDTDLFSQFSHWLEEVPSIARVEQAVKTVASVDAKTTIELAKLRTQIKKLEKQLNIRADQQKQTQLEQL
QLRLNQILEV

Sequences:

>Translated_250_residues
MPQTQCGLCGHRDGCLPYAKSMIEGEAANKCIPGGQPVADALAQLLNRPHLKAEQSIWPIQSDGRPQRMKAIIREDECIG
CTKCISACPVDAIIGSGKLMHTVLTDLCTGCELCIPPCPVDCIDLVEDREPLPTIAERNNEQQQLRQRYYAHIQREEQRS
IHRKGPIVRAEIDTDLFSQFSHWLEEVPSIARVEQAVKTVASVDAKTTIELAKLRTQIKKLEKQLNIRADQQKQTQLEQL
QLRLNQILEV
>Mature_249_residues
PQTQCGLCGHRDGCLPYAKSMIEGEAANKCIPGGQPVADALAQLLNRPHLKAEQSIWPIQSDGRPQRMKAIIREDECIGC
TKCISACPVDAIIGSGKLMHTVLTDLCTGCELCIPPCPVDCIDLVEDREPLPTIAERNNEQQQLRQRYYAHIQREEQRSI
HRKGPIVRAEIDTDLFSQFSHWLEEVPSIARVEQAVKTVASVDAKTTIELAKLRTQIKKLEKQLNIRADQQKQTQLEQLQ
LRLNQILEV

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG2878

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfB

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787915, Length=129, Percent_Identity=51.937984496124, Blast_Score=128, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010207
- InterPro:   IPR016463
- InterPro:   IPR007202 [H]

Pfam domain/function: PF00037 Fer4; PF04060 FeS [H]

EC number: NA

Molecular weight: Translated: 28122; Mature: 27991

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
5.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCC
>Mature Secondary Structure 
PQTQCGLCGHRDGCLPYAKSMIEGEAANKCIPGGQPVADALAQLLNRPHLKAEQSIWPI
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHCCCCCC
QSDGRPQRMKAIIREDECIGCTKCISACPVDAIIGSGKLMHTVLTDLCTGCELCIPPCPV
CCCCCHHHHHHHHHCCCCCCHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCH
DCIDLVEDREPLPTIAERNNEQQQLRQRYYAHIQREEQRSIHRKGPIVRAEIDTDLFSQF
HHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHH
SHWLEEVPSIARVEQAVKTVASVDAKTTIELAKLRTQIKKLEKQLNIRADQQKQTQLEQL
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
QLRLNQILEV
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA