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The map label for this gene is nth [H]

Identifier: 50084302

GI number: 50084302

Start: 1095871

End: 1096536

Strand: Reverse

Name: nth [H]

Synonym: ACIAD1108

Alternate gene names: 50084302

Gene position: 1096536-1095871 (Counterclockwise)

Preceding gene: 50084303

Following gene: 50084297

Centisome position: 30.47

GC content: 40.39

Gene sequence:

>666_bases
ATGACCAAAAAACAGATTCGGACGTTTTTTGAACGCCTTAGAGCGCAGAGACCTTATCCCCAAACTGAGCTAAAATATTC
AAGCCCTTTCGAGCTTTTAATTGCAGTGATGCTCTCGGCACAAGCAACGGATGTTAGTGTAAATAAAGCCACAGACAAAC
TCTATCCAGTTGCCAATACTGCTGCACAGATTTATGCGCTAGGGGTCGAAGGGCTTAAACAATATATCAAAACCATTGGT
TTGTATAATGCCAAAGCTGAGAATGTGATTAAAACTTGTCAAATTCTTTTAGAAAAGCATCAGGGAGAAGTTCCACAAAC
CCGTGCTGAGCTTGAGGCTTTACCAGGTGTAGGGCGTAAAACAGCCAATGTTGTTCTTAATACCGCATTCGGACAACCCA
CAATGGCTGTAGATACACATATTTTTCGTGTAGGAAATAGAACTGGACTAGCTATTGGAAAAAATGTACTTGAAGTTGAA
CATCGTTTGATCAAGGTAATTCCTAAAGAATTTATAATCGATGCTCATCACTGGCTGATTTTACACGGACGTTATTGTTG
TATTGCTCGCAAACCTAAATGTGCAGAATGTGTTGTTTCAGATGTGTGTAATTGGCCAGATCGCTTTGAATATGGTGCAA
CACGCAAAATGGCAGTCAAAGAGTGA

Upstream 100 bases:

>100_bases
ACAACTCAACATTAGAGCTGATCAACAAAAGCAAACGCAACTTGAACAGTTACAACTACGCTTAAATCAGATTCTGGAGG
TCTAGATGGCGGTAAAAAAT

Downstream 100 bases:

>100_bases
TCATCACTCTTTATCTTTTAATTTTGGATGCAGCGTCCGTTCTTTAGGATGCTGATTCAAACGCCTTTCTTGCATTTCCT
GCTTTTGAATCTGACGCAGC

Product: endonuclease III DNA glycosylase/apyrimidinic (AP) lyase

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MTKKQIRTFFERLRAQRPYPQTELKYSSPFELLIAVMLSAQATDVSVNKATDKLYPVANTAAQIYALGVEGLKQYIKTIG
LYNAKAENVIKTCQILLEKHQGEVPQTRAELEALPGVGRKTANVVLNTAFGQPTMAVDTHIFRVGNRTGLAIGKNVLEVE
HRLIKVIPKEFIIDAHHWLILHGRYCCIARKPKCAECVVSDVCNWPDRFEYGATRKMAVKE

Sequences:

>Translated_221_residues
MTKKQIRTFFERLRAQRPYPQTELKYSSPFELLIAVMLSAQATDVSVNKATDKLYPVANTAAQIYALGVEGLKQYIKTIG
LYNAKAENVIKTCQILLEKHQGEVPQTRAELEALPGVGRKTANVVLNTAFGQPTMAVDTHIFRVGNRTGLAIGKNVLEVE
HRLIKVIPKEFIIDAHHWLILHGRYCCIARKPKCAECVVSDVCNWPDRFEYGATRKMAVKE
>Mature_220_residues
TKKQIRTFFERLRAQRPYPQTELKYSSPFELLIAVMLSAQATDVSVNKATDKLYPVANTAAQIYALGVEGLKQYIKTIGL
YNAKAENVIKTCQILLEKHQGEVPQTRAELEALPGVGRKTANVVLNTAFGQPTMAVDTHIFRVGNRTGLAIGKNVLEVEH
RLIKVIPKEFIIDAHHWLILHGRYCCIARKPKCAECVVSDVCNWPDRFEYGATRKMAVKE

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=29.3785310734463, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1787920, Length=210, Percent_Identity=69.5238095238095, Blast_Score=311, Evalue=3e-86,
Organism=Escherichia coli, GI1789331, Length=127, Percent_Identity=32.2834645669291, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=32.5714285714286, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324530, Length=188, Percent_Identity=25.531914893617, Blast_Score=64, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550361, Length=178, Percent_Identity=30.8988764044944, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24868; Mature: 24737

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKQIRTFFERLRAQRPYPQTELKYSSPFELLIAVMLSAQATDVSVNKATDKLYPVANT
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHH
AAQIYALGVEGLKQYIKTIGLYNAKAENVIKTCQILLEKHQGEVPQTRAELEALPGVGRK
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
TANVVLNTAFGQPTMAVDTHIFRVGNRTGLAIGKNVLEVEHRLIKVIPKEFIIDAHHWLI
HHHEEEECCCCCCCHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LHGRYCCIARKPKCAECVVSDVCNWPDRFEYGATRKMAVKE
EECCEEEEECCCCHHHHHHHHHCCCCCHHCCCCCCCCCCCC
>Mature Secondary Structure 
TKKQIRTFFERLRAQRPYPQTELKYSSPFELLIAVMLSAQATDVSVNKATDKLYPVANT
CHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHH
AAQIYALGVEGLKQYIKTIGLYNAKAENVIKTCQILLEKHQGEVPQTRAELEALPGVGRK
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
TANVVLNTAFGQPTMAVDTHIFRVGNRTGLAIGKNVLEVEHRLIKVIPKEFIIDAHHWLI
HHHEEEECCCCCCCHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LHGRYCCIARKPKCAECVVSDVCNWPDRFEYGATRKMAVKE
EECCEEEEECCCCHHHHHHHHHCCCCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]