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The map label for this gene is rubB

Identifier: 50084265

GI number: 50084265

Start: 1061554

End: 1062735

Strand: Reverse

Name: rubB

Synonym: ACIAD1065

Alternate gene names: 50084265

Gene position: 1062735-1061554 (Counterclockwise)

Preceding gene: 50084266

Following gene: 50084264

Centisome position: 29.53

GC content: 44.5

Gene sequence:

>1182_bases
ATGCACCCAATCGTCATTATCGGATCAGGAATGGCAGGTTATACCCTTGCTCGTGAATTTCGCAAGCTTAATCCTGAACA
TGAACTTGTCATGATTTGTGCTGACGATGCAGTGAATTATGCAAAACCAACCTTATCTAATGCGCTTTCTGGCAACAAAG
CACCAGAACAGATTCCATTAGGTGATGCAGAAAAAATGAGTACCCAGCTCAAGCTGCAAATTTTGTCAGAAACTTGGGTA
AAAGCCATTAATCCAGAAACTCATGAATTAAAATTAGAAAAAAATGGTCAGGAAACGATTCAGCCCTATTCCAAACTGGT
TCTGGCTGTAGGCGCCAATCCAACCCGTTTAGCCATTGCCGGTGATGGTAGTGATGATATTCATGTGGTGAACTCTCTTA
TCGATTATCGCGCCTTTCGTGAAAATCTTGCAAAACGTCAAGATAAGCGTGTTGTCATTCTAGGTGCAGGTCTTATTGGT
TGTGAATTTGCCAATGATTTACAGCATACTGGGCATCAGGTGACAGTCATCGACTTGTCTCCTCGCCCACTGGGTCGTCT
ATTACCTGCGCATATTGCAGATGCTTTTCAGAAAAATCTGGAAGAAAGCGGTATTCACTTTGTACTTTCAACCACAGTAG
AAAAAGTATCTAAAATTAACGATGGTCAAGATTATGCTGTCACACTGGCAAATGGTCAGACGCTAGTGGCCGACATTGTT
TTATCTGCAATCGGATTACAACCAAACATTGATCTGGCAAAACACGCAGGCGTGCATACCAGTCGAGGTATTTTGACTAA
TAGCCTCCTAGAAACCAACCTTGAAGACATCTATGCAATAGGCGACTGTGCTGAAGTTAATGGAACCTTGCTCCCCTACG
TGATGCCGATCATGCAGCAGGCCCGCGCCCTCGCGAAAACCTTGAGTGGTGAAACAACACATGTTCACTATCCTGCCATG
CCAGTTGCTGTAAAAACGCCTGCAGCCCCGCTCACCGTACTTCCTGTACCTGTGGATGTAGATGTAAACTGGGAAACAGA
AGAATTTGAAGATGGTATGCTTGCCAAAGCTATCGACAATACCGATACACTCAGAGGTTTTGTACTATTAGGTGCTACGG
CGGGTAAACAGCGCCTGACACTCACCAAACTGGTTCCAGACTTGATTCCCGCACAGCTTTAA

Upstream 100 bases:

>100_bases
AATGATCGAAGTTTAATCGGTATTTCAAAAGACCATGTCCATGAAACATGGTCTTTTTAATTTAATCTTAGTTTTTATTA
CCTTACTACGGAGAATAATA

Downstream 100 bases:

>100_bases
GGTTTAAATAGCAAGGACATTCATCTGAGTGTCCTTTTTAGGAACGGATCATAAAATGAATAGCGCTTTGCAATTTAGCT
CTTCACCTTATACTTCATTT

Product: rubredoxin-NAD(+) reductase

Products: NA

Alternate protein names: RdxR

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MHPIVIIGSGMAGYTLAREFRKLNPEHELVMICADDAVNYAKPTLSNALSGNKAPEQIPLGDAEKMSTQLKLQILSETWV
KAINPETHELKLEKNGQETIQPYSKLVLAVGANPTRLAIAGDGSDDIHVVNSLIDYRAFRENLAKRQDKRVVILGAGLIG
CEFANDLQHTGHQVTVIDLSPRPLGRLLPAHIADAFQKNLEESGIHFVLSTTVEKVSKINDGQDYAVTLANGQTLVADIV
LSAIGLQPNIDLAKHAGVHTSRGILTNSLLETNLEDIYAIGDCAEVNGTLLPYVMPIMQQARALAKTLSGETTHVHYPAM
PVAVKTPAAPLTVLPVPVDVDVNWETEEFEDGMLAKAIDNTDTLRGFVLLGATAGKQRLTLTKLVPDLIPAQL

Sequences:

>Translated_393_residues
MHPIVIIGSGMAGYTLAREFRKLNPEHELVMICADDAVNYAKPTLSNALSGNKAPEQIPLGDAEKMSTQLKLQILSETWV
KAINPETHELKLEKNGQETIQPYSKLVLAVGANPTRLAIAGDGSDDIHVVNSLIDYRAFRENLAKRQDKRVVILGAGLIG
CEFANDLQHTGHQVTVIDLSPRPLGRLLPAHIADAFQKNLEESGIHFVLSTTVEKVSKINDGQDYAVTLANGQTLVADIV
LSAIGLQPNIDLAKHAGVHTSRGILTNSLLETNLEDIYAIGDCAEVNGTLLPYVMPIMQQARALAKTLSGETTHVHYPAM
PVAVKTPAAPLTVLPVPVDVDVNWETEEFEDGMLAKAIDNTDTLRGFVLLGATAGKQRLTLTKLVPDLIPAQL
>Mature_393_residues
MHPIVIIGSGMAGYTLAREFRKLNPEHELVMICADDAVNYAKPTLSNALSGNKAPEQIPLGDAEKMSTQLKLQILSETWV
KAINPETHELKLEKNGQETIQPYSKLVLAVGANPTRLAIAGDGSDDIHVVNSLIDYRAFRENLAKRQDKRVVILGAGLIG
CEFANDLQHTGHQVTVIDLSPRPLGRLLPAHIADAFQKNLEESGIHFVLSTTVEKVSKINDGQDYAVTLANGQTLVADIV
LSAIGLQPNIDLAKHAGVHTSRGILTNSLLETNLEDIYAIGDCAEVNGTLLPYVMPIMQQARALAKTLSGETTHVHYPAM
PVAVKTPAAPLTVLPVPVDVDVNWETEEFEDGMLAKAIDNTDTLRGFVLLGATAGKQRLTLTKLVPDLIPAQL

Specific function: Involved in the hydrocarbon hydroxylating system, which transfers electrons from NADH to rubredoxin reductase and then through rubredoxin to alkane 1 monooxygenase

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family

Homologues:

Organism=Homo sapiens, GI226437568, Length=282, Percent_Identity=28.3687943262411, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI65787454, Length=282, Percent_Identity=28.3687943262411, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI21389617, Length=282, Percent_Identity=28.3687943262411, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI91199540, Length=190, Percent_Identity=28.4210526315789, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4757732, Length=316, Percent_Identity=22.4683544303797, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI22202629, Length=316, Percent_Identity=22.4683544303797, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1789065, Length=366, Percent_Identity=36.3387978142077, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI1788892, Length=281, Percent_Identity=28.1138790035587, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789765, Length=307, Percent_Identity=26.0586319218241, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17559934, Length=286, Percent_Identity=28.6713286713287, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24639257, Length=283, Percent_Identity=23.6749116607774, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI281359715, Length=283, Percent_Identity=23.6749116607774, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281359713, Length=283, Percent_Identity=23.6749116607774, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24639250, Length=283, Percent_Identity=23.6749116607774, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI18543267, Length=283, Percent_Identity=23.6749116607774, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24639252, Length=283, Percent_Identity=23.6749116607774, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21358499, Length=366, Percent_Identity=24.8633879781421, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24585130, Length=283, Percent_Identity=21.5547703180212, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RURE_ACIAD (P42454)

Other databases:

- EMBL:   Z46863
- EMBL:   CR543861
- EMBL:   X88895
- RefSeq:   YP_045775.1
- ProteinModelPortal:   P42454
- SMR:   P42454
- STRING:   P42454
- GeneID:   2880553
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1065
- NMPDR:   fig|62977.3.peg.1214
- eggNOG:   COG0446
- HOGENOM:   HBG542563
- OMA:   WRENDES
- PhylomeDB:   P42454
- ProtClustDB:   CLSK707186
- BioCyc:   ASP62977:ACIAD1065-MONOMER
- GO:   GO:0005737
- InterPro:   IPR013027
- InterPro:   IPR001327
- PRINTS:   PR00368

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2

EC number: =1.18.1.1

Molecular weight: Translated: 42464; Mature: 42464

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: BINDING 42-42 BINDING 80-80 BINDING 162-162 BINDING 325-325

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPIVIIGSGMAGYTLAREFRKLNPEHELVMICADDAVNYAKPTLSNALSGNKAPEQIPL
CCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCC
GDAEKMSTQLKLQILSETWVKAINPETHELKLEKNGQETIQPYSKLVLAVGANPTRLAIA
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHEEEEECCCCEEEEEE
GDGSDDIHVVNSLIDYRAFRENLAKRQDKRVVILGAGLIGCEFANDLQHTGHQVTVIDLS
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEHHHHHHHHHHCCCEEEEEEEC
PRPLGRLLPAHIADAFQKNLEESGIHFVLSTTVEKVSKINDGQDYAVTLANGQTLVADIV
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCEEEEEECCCCHHHHHH
LSAIGLQPNIDLAKHAGVHTSRGILTNSLLETNLEDIYAIGDCAEVNGTLLPYVMPIMQQ
HHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHCCHHHHEEECCHHHCCCCHHHHHHHHHHH
ARALAKTLSGETTHVHYPAMPVAVKTPAAPLTVLPVPVDVDVNWETEEFEDGMLAKAIDN
HHHHHHHHCCCCEEEECCCCCEEEECCCCCEEEEEEEEEEECCCCHHHHCCCCHHHHCCC
TDTLRGFVLLGATAGKQRLTLTKLVPDLIPAQL
CHHEEEEEEEECCCCCCEEEHHHHHHHHHCCCC
>Mature Secondary Structure
MHPIVIIGSGMAGYTLAREFRKLNPEHELVMICADDAVNYAKPTLSNALSGNKAPEQIPL
CCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCC
GDAEKMSTQLKLQILSETWVKAINPETHELKLEKNGQETIQPYSKLVLAVGANPTRLAIA
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHEEEEECCCCEEEEEE
GDGSDDIHVVNSLIDYRAFRENLAKRQDKRVVILGAGLIGCEFANDLQHTGHQVTVIDLS
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEHHHHHHHHHHCCCEEEEEEEC
PRPLGRLLPAHIADAFQKNLEESGIHFVLSTTVEKVSKINDGQDYAVTLANGQTLVADIV
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCEEEEEECCCCHHHHHH
LSAIGLQPNIDLAKHAGVHTSRGILTNSLLETNLEDIYAIGDCAEVNGTLLPYVMPIMQQ
HHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHCCHHHHEEECCHHHCCCCHHHHHHHHHHH
ARALAKTLSGETTHVHYPAMPVAVKTPAAPLTVLPVPVDVDVNWETEEFEDGMLAKAIDN
HHHHHHHHCCCCEEEECCCCCEEEECCCCCEEEEEEEEEEECCCCHHHHCCCCHHHHCCC
TDTLRGFVLLGATAGKQRLTLTKLVPDLIPAQL
CHHEEEEEEEECCCCCCEEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7670642