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The map label for this gene is phaB [H]

Identifier: 50084264

GI number: 50084264

Start: 1060560

End: 1061498

Strand: Reverse

Name: phaB [H]

Synonym: ACIAD1064

Alternate gene names: 50084264

Gene position: 1061498-1060560 (Counterclockwise)

Preceding gene: 50084265

Following gene: 50084263

Centisome position: 29.5

GC content: 40.26

Gene sequence:

>939_bases
ATGAATAGCGCTTTGCAATTTAGCTCTTCACCTTATACTTCATTTATGCATGAAACTAAGGTAAAGCTAAGCAACGGATT
AGAGCTACACGTCGAAGTGGGCGGAAATCCCGATCATCCCACTATTCTTCTGATTATGGGTTTGGGTGCCCAAATGTTAT
TCTGGCCTGATTTTTTCTGTAAATCATTAATTGATCAGGGCTTTTATGTGATCCGATTTGACAATCGTGACATTGGATTG
TCATCCAAAATTCGCCACAAAGGCAAACGCTTAAATACCTTAAAGCTGATGAGCCGTTTTACGCTGGGATTGGGCAATCA
GGGTGCACCTTACACACTTTATGATATGGCAGAAGACGTTTCGCTTTTACTTGAAGCGATGCGGATTAAAAAAGTCAATG
TGATTGGTGCTTCAATGGGTGGCATGATTGCGCAGATTATTGCAGCAAAATATCCAGAGAAAGTTGAAAAACTGGCATTG
ATGTTTACCAGTAATAATCAGCCGCTCCTTCCGCCACCTTTCCCTAAACAACTGTTTAGTTTGATTGGAAAACCCAAATC
AAGCGATGAAGATGGTATCATTAACCACAGTCTAAAATTATTTGAGATCATTGGTTCACCAGGTTATGTGAATCAGGTCG
AAGCAATACAGACTGCACGTAAACTGTATCAACGAAGTTATCACCCTGCGGGTGTACTTCAACAGTTTTTAGCAATATTA
TGTACGGGGTCCTTACTACAACTGGACAAACAGATTTCTCAACCAACATTGGTGATCCATGGTTCGAGAGACCGTTTACT
TCCTCCAAGTCATGGTAAGGCAGTTGCGAAAGCAATTAAAGGTGCTAAGTTTGAATTAATTCAGGGAATGGGACATGATA
TTCCACCACATTTTATTCCCCAACTTAGTTATCTCTTTGCTCATCACTTTAGATCATAA

Upstream 100 bases:

>100_bases
ACACTCACCAAACTGGTTCCAGACTTGATTCCCGCACAGCTTTAAGGTTTAAATAGCAAGGACATTCATCTGAGTGTCCT
TTTTAGGAACGGATCATAAA

Downstream 100 bases:

>100_bases
GCACTAGGACATTATGGCTGCATTACCCTCCTTAAGACAGCTGTCATATTTAGTTACGCTGTCGGAAACATTGCACTTTA
CAGAAGCAGCACGCCGTTCA

Product: hydrolase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MNSALQFSSSPYTSFMHETKVKLSNGLELHVEVGGNPDHPTILLIMGLGAQMLFWPDFFCKSLIDQGFYVIRFDNRDIGL
SSKIRHKGKRLNTLKLMSRFTLGLGNQGAPYTLYDMAEDVSLLLEAMRIKKVNVIGASMGGMIAQIIAAKYPEKVEKLAL
MFTSNNQPLLPPPFPKQLFSLIGKPKSSDEDGIINHSLKLFEIIGSPGYVNQVEAIQTARKLYQRSYHPAGVLQQFLAIL
CTGSLLQLDKQISQPTLVIHGSRDRLLPPSHGKAVAKAIKGAKFELIQGMGHDIPPHFIPQLSYLFAHHFRS

Sequences:

>Translated_312_residues
MNSALQFSSSPYTSFMHETKVKLSNGLELHVEVGGNPDHPTILLIMGLGAQMLFWPDFFCKSLIDQGFYVIRFDNRDIGL
SSKIRHKGKRLNTLKLMSRFTLGLGNQGAPYTLYDMAEDVSLLLEAMRIKKVNVIGASMGGMIAQIIAAKYPEKVEKLAL
MFTSNNQPLLPPPFPKQLFSLIGKPKSSDEDGIINHSLKLFEIIGSPGYVNQVEAIQTARKLYQRSYHPAGVLQQFLAIL
CTGSLLQLDKQISQPTLVIHGSRDRLLPPSHGKAVAKAIKGAKFELIQGMGHDIPPHFIPQLSYLFAHHFRS
>Mature_312_residues
MNSALQFSSSPYTSFMHETKVKLSNGLELHVEVGGNPDHPTILLIMGLGAQMLFWPDFFCKSLIDQGFYVIRFDNRDIGL
SSKIRHKGKRLNTLKLMSRFTLGLGNQGAPYTLYDMAEDVSLLLEAMRIKKVNVIGASMGGMIAQIIAAKYPEKVEKLAL
MFTSNNQPLLPPPFPKQLFSLIGKPKSSDEDGIINHSLKLFEIIGSPGYVNQVEAIQTARKLYQRSYHPAGVLQQFLAIL
CTGSLLQLDKQISQPTLVIHGSRDRLLPPSHGKAVAKAIKGAKFELIQGMGHDIPPHFIPQLSYLFAHHFRS

Specific function: PHA depolymerase is in fact a lipase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR011942 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: NA

Molecular weight: Translated: 34681; Mature: 34681

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSALQFSSSPYTSFMHETKVKLSNGLELHVEVGGNPDHPTILLIMGLGAQMLFWPDFFC
CCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHH
KSLIDQGFYVIRFDNRDIGLSSKIRHKGKRLNTLKLMSRFTLGLGNQGAPYTLYDMAEDV
HHHHHCCEEEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHH
SLLLEAMRIKKVNVIGASMGGMIAQIIAAKYPEKVEKLALMFTSNNQPLLPPPFPKQLFS
HHHHHHHHHHHHEEECCHHHHHHHHHHHHHCHHHHHHEEEEEECCCCCCCCCCCHHHHHH
LIGKPKSSDEDGIINHSLKLFEIIGSPGYVNQVEAIQTARKLYQRSYHPAGVLQQFLAIL
HHCCCCCCCCCCHHHCCCEEEHEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
CTGSLLQLDKQISQPTLVIHGSRDRLLPPSHGKAVAKAIKGAKFELIQGMGHDIPPHFIP
HCCHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCHHHH
QLSYLFAHHFRS
HHHHHHHHHHCC
>Mature Secondary Structure
MNSALQFSSSPYTSFMHETKVKLSNGLELHVEVGGNPDHPTILLIMGLGAQMLFWPDFFC
CCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHH
KSLIDQGFYVIRFDNRDIGLSSKIRHKGKRLNTLKLMSRFTLGLGNQGAPYTLYDMAEDV
HHHHHCCEEEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHH
SLLLEAMRIKKVNVIGASMGGMIAQIIAAKYPEKVEKLALMFTSNNQPLLPPPFPKQLFS
HHHHHHHHHHHHEEECCHHHHHHHHHHHHHCHHHHHHEEEEEECCCCCCCCCCCHHHHHH
LIGKPKSSDEDGIINHSLKLFEIIGSPGYVNQVEAIQTARKLYQRSYHPAGVLQQFLAIL
HHCCCCCCCCCCHHHCCCEEEHEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
CTGSLLQLDKQISQPTLVIHGSRDRLLPPSHGKAVAKAIKGAKFELIQGMGHDIPPHFIP
HCCHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCHHHH
QLSYLFAHHFRS
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1989978 [H]