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The map label for this gene is pit [H]

Identifier: 50084248

GI number: 50084248

Start: 1038795

End: 1040423

Strand: Direct

Name: pit [H]

Synonym: ACIAD1047

Alternate gene names: 50084248

Gene position: 1038795-1040423 (Clockwise)

Preceding gene: 50084247

Following gene: 50084249

Centisome position: 28.87

GC content: 40.64

Gene sequence:

>1629_bases
ATGAATTCAAATCTACCCCCTGTTTCGGATTCAAAGCTTGTGGAACCTTCCCTTGCAAAATCGACGAATGTACATGTACC
TACACCGAAATTTTTTATGCCGGTGTTTTTTACAATTATTATTGCAACCCTGATTTACATTGGATTTCAGGTGAATGCTG
ATTTGGCGCACGTACCGCCTTTAAATTTATACTCTGTTATTTTACTTTCTACTGCATTATTGATTGCATTAGGTTTTGAG
TTTGTTAATGGCTTTCATGACACAGCAAATGCCGTTGCAACGGTGATTTATACCAATGCATTGCCTGCACCTGTAGCCGT
GATGTGGGCAGGTTTCTGTAATTTTCTGGGTGTTATGGTGGCTAGCGGCGCCGTAGCATACGGAATCATTGCATTATTGC
CCGTCGAACTGATTATGAATGTCAGTAGTGGCGCTGGCTTTGCCATGGTTTTTGCACTTTTAATTGCCGCGATTATCTGG
AACTTAGGAACATGGTTTCTTGGTATTCCAGCTTCAAGTTCTCACACCCTGATTGGTTCAATTTTGGGTGTAGGTATCAT
GAACCATATTTTGAATGCATCTTCTGGAAGCAGTGGCATCGATATGGATCAAGTCATTAAAGTGGGTAAAGCATTATTAT
TTTCTCCTTTAATTGGTTTTGCTTTTGCAGCAGTCTTATTCCTTTTGGTCAAGAAAGTTTTTAGACGTCAACTTGAACTT
TTTCAGCCACCTGAAGGCAATAAACCGCCGCCACCCATCATTCGTGCGATTCTGATTTTTACCTGTACTGGTGTAAGTTT
TGCTCACGGTTCTAATGACGGCCAAAAGGGTATGGGTCTGATCATGCTCATTTTGATTGGATTGGTACCACTCGCGTATT
CATTGAATAAAAATCTTGATGCACAATATTTAAAGTCTTTTGAGCAACTTTCGAGCGAAACTGCAATCGTTTTGCATGCA
AATCAGGCTGAGATGAAAGATGAAAAAGCGCGTGAAATTCTGACCAAATATATTCAGACCAAACAGCAAACGCCAGAAGT
TGTTCCTGCTTTGGCAAGTATTACAGATCATCTTGGTCAGCGCGTTGCAACGTATGGTGATTTAAAAGATATTCCAGAAG
TTGCAGTGAGTGAAATCCGCAATGATATGTATTTAAGTACAACCACTTTTAAACGTCTGGATAAAGCTGAAGCGCTTCCA
GCAATGACACCTCAACAAGAAGGTGTTGTAAAAGAATACCGTAGTAATCTGGATGGTTTCTTACAATACATTCCAAACTG
GGTGAAGGTTGCTGTAGCATTAGCTTTAGGTCTTGGCACCATGGTGGGCTGGAAGCGTATTGTGGTGACTGTAGGTGAGC
GTATTGGTAAAAACCATATGACTTACGGTCAGGGAATGTCTGCTGAATTAGTTGCAATGAGTACCATTGCTGCTGCCGAT
GGTTTGGGCATGCCAGTATCAACGACGCATGTATTAAATAGTGCAGTTGCTGGTACCATGGTTGCCAATAAATCAGGTCT
AAACTTTAATACAGTTAAAACCATTATTTCGGCATGGATTTTTACCTTGCCAGCCACGATCTGTTTGTCAGGTGGCTTAT
ATTGGTTATTTTTACAGTTTGTAAATTAA

Upstream 100 bases:

>100_bases
ATGGATAAAAATTGACTTCATAAAAGGGCTGTTTGTCATATAATTGTCATAATTTAATTTAAGAATAAGGGGATTTTATT
AATCCTCTTGAACCGTCTGC

Downstream 100 bases:

>100_bases
ATTTGAAAATGCAGAAGAGGCCACTAAAGAGTGGCCTTTTTTTTATCTCTGGTTTATTTTAACGGTTTATTATTTATTTT
GTATTTGGGCTGTGCATGAT

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MNSNLPPVSDSKLVEPSLAKSTNVHVPTPKFFMPVFFTIIIATLIYIGFQVNADLAHVPPLNLYSVILLSTALLIALGFE
FVNGFHDTANAVATVIYTNALPAPVAVMWAGFCNFLGVMVASGAVAYGIIALLPVELIMNVSSGAGFAMVFALLIAAIIW
NLGTWFLGIPASSSHTLIGSILGVGIMNHILNASSGSSGIDMDQVIKVGKALLFSPLIGFAFAAVLFLLVKKVFRRQLEL
FQPPEGNKPPPPIIRAILIFTCTGVSFAHGSNDGQKGMGLIMLILIGLVPLAYSLNKNLDAQYLKSFEQLSSETAIVLHA
NQAEMKDEKAREILTKYIQTKQQTPEVVPALASITDHLGQRVATYGDLKDIPEVAVSEIRNDMYLSTTTFKRLDKAEALP
AMTPQQEGVVKEYRSNLDGFLQYIPNWVKVAVALALGLGTMVGWKRIVVTVGERIGKNHMTYGQGMSAELVAMSTIAAAD
GLGMPVSTTHVLNSAVAGTMVANKSGLNFNTVKTIISAWIFTLPATICLSGGLYWLFLQFVN

Sequences:

>Translated_542_residues
MNSNLPPVSDSKLVEPSLAKSTNVHVPTPKFFMPVFFTIIIATLIYIGFQVNADLAHVPPLNLYSVILLSTALLIALGFE
FVNGFHDTANAVATVIYTNALPAPVAVMWAGFCNFLGVMVASGAVAYGIIALLPVELIMNVSSGAGFAMVFALLIAAIIW
NLGTWFLGIPASSSHTLIGSILGVGIMNHILNASSGSSGIDMDQVIKVGKALLFSPLIGFAFAAVLFLLVKKVFRRQLEL
FQPPEGNKPPPPIIRAILIFTCTGVSFAHGSNDGQKGMGLIMLILIGLVPLAYSLNKNLDAQYLKSFEQLSSETAIVLHA
NQAEMKDEKAREILTKYIQTKQQTPEVVPALASITDHLGQRVATYGDLKDIPEVAVSEIRNDMYLSTTTFKRLDKAEALP
AMTPQQEGVVKEYRSNLDGFLQYIPNWVKVAVALALGLGTMVGWKRIVVTVGERIGKNHMTYGQGMSAELVAMSTIAAAD
GLGMPVSTTHVLNSAVAGTMVANKSGLNFNTVKTIISAWIFTLPATICLSGGLYWLFLQFVN
>Mature_542_residues
MNSNLPPVSDSKLVEPSLAKSTNVHVPTPKFFMPVFFTIIIATLIYIGFQVNADLAHVPPLNLYSVILLSTALLIALGFE
FVNGFHDTANAVATVIYTNALPAPVAVMWAGFCNFLGVMVASGAVAYGIIALLPVELIMNVSSGAGFAMVFALLIAAIIW
NLGTWFLGIPASSSHTLIGSILGVGIMNHILNASSGSSGIDMDQVIKVGKALLFSPLIGFAFAAVLFLLVKKVFRRQLEL
FQPPEGNKPPPPIIRAILIFTCTGVSFAHGSNDGQKGMGLIMLILIGLVPLAYSLNKNLDAQYLKSFEQLSSETAIVLHA
NQAEMKDEKAREILTKYIQTKQQTPEVVPALASITDHLGQRVATYGDLKDIPEVAVSEIRNDMYLSTTTFKRLDKAEALP
AMTPQQEGVVKEYRSNLDGFLQYIPNWVKVAVALALGLGTMVGWKRIVVTVGERIGKNHMTYGQGMSAELVAMSTIAAAD
GLGMPVSTTHVLNSAVAGTMVANKSGLNFNTVKTIISAWIFTLPATICLSGGLYWLFLQFVN

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=489, Percent_Identity=41.5132924335378, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1789360, Length=504, Percent_Identity=40.8730158730159, Blast_Score=369, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 58218; Mature: 58218

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSNLPPVSDSKLVEPSLAKSTNVHVPTPKFFMPVFFTIIIATLIYIGFQVNADLAHVPP
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LNLYSVILLSTALLIALGFEFVNGFHDTANAVATVIYTNALPAPVAVMWAGFCNFLGVMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ASGAVAYGIIALLPVELIMNVSSGAGFAMVFALLIAAIIWNLGTWFLGIPASSSHTLIGS
HCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHEEECCCCCCHHHHHH
ILGVGIMNHILNASSGSSGIDMDQVIKVGKALLFSPLIGFAFAAVLFLLVKKVFRRQLEL
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQPPEGNKPPPPIIRAILIFTCTGVSFAHGSNDGQKGMGLIMLILIGLVPLAYSLNKNLD
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
AQYLKSFEQLSSETAIVLHANQAEMKDEKAREILTKYIQTKQQTPEVVPALASITDHLGQ
HHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RVATYGDLKDIPEVAVSEIRNDMYLSTTTFKRLDKAEALPAMTPQQEGVVKEYRSNLDGF
HHHHHCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
LQYIPNWVKVAVALALGLGTMVGWKRIVVTVGERIGKNHMTYGQGMSAELVAMSTIAAAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
GLGMPVSTTHVLNSAVAGTMVANKSGLNFNTVKTIISAWIFTLPATICLSGGLYWLFLQF
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VN
CC
>Mature Secondary Structure
MNSNLPPVSDSKLVEPSLAKSTNVHVPTPKFFMPVFFTIIIATLIYIGFQVNADLAHVPP
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LNLYSVILLSTALLIALGFEFVNGFHDTANAVATVIYTNALPAPVAVMWAGFCNFLGVMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ASGAVAYGIIALLPVELIMNVSSGAGFAMVFALLIAAIIWNLGTWFLGIPASSSHTLIGS
HCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHEEECCCCCCHHHHHH
ILGVGIMNHILNASSGSSGIDMDQVIKVGKALLFSPLIGFAFAAVLFLLVKKVFRRQLEL
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQPPEGNKPPPPIIRAILIFTCTGVSFAHGSNDGQKGMGLIMLILIGLVPLAYSLNKNLD
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
AQYLKSFEQLSSETAIVLHANQAEMKDEKAREILTKYIQTKQQTPEVVPALASITDHLGQ
HHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RVATYGDLKDIPEVAVSEIRNDMYLSTTTFKRLDKAEALPAMTPQQEGVVKEYRSNLDGF
HHHHHCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
LQYIPNWVKVAVALALGLGTMVGWKRIVVTVGERIGKNHMTYGQGMSAELVAMSTIAAAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
GLGMPVSTTHVLNSAVAGTMVANKSGLNFNTVKTIISAWIFTLPATICLSGGLYWLFLQF
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]