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The map label for this gene is pgaB [H]

Identifier: 50084231

GI number: 50084231

Start: 1020509

End: 1022584

Strand: Direct

Name: pgaB [H]

Synonym: ACIAD1029

Alternate gene names: 50084231

Gene position: 1020509-1022584 (Clockwise)

Preceding gene: 50084230

Following gene: 50084232

Centisome position: 28.36

GC content: 40.41

Gene sequence:

>2076_bases
ATGCTAACTCGCTTCCCTTTGAAACTAATCACATTAAGTGTTTCCACAGTCTTAAGCTTATCTTTAGGCTGTGCACATGC
TGAAACTGGTACATCGACCAATGACAATCCTGCAGTGAAGGAAGTTCCGCTCGTAACCAATCCGCATACTGGAGAAGAAG
CCGAAGAAGCACCAAAAATTGATGTGTCAACGCTTACTATTATTGGATATCATGAAATTACGTCAAAAGAAGCGATAATT
CCTGATTATGCCGTAACACCACAGCGTTTTGAAACACATTTAGATTGGCTAATTAAAAATGGTTATCATTTTATTAGTGT
TGACCAATTAATTAAGGCCAATGAAGGTAAAATAAAACTTCCAGCTAAACCCGTCTTATTAACTGTCGACGATGGTTATC
AATCATTTTATGACTATGCTTATCCAATTTTAAAAGCACGTAAAATTCCTGTTGTTTTAGCCGTTGTCGGTAAATGGCTC
AATGTCAAGCCAAATCAAATGGTACAGTTTGGTGACGATAAGCTTGCGCGAAATAAATTATTAACATGGGCTGAAATTAA
GGAAATGCAGGATAGTGGTATTGTCGAAATTGGCAGCCATAGCTTTGACTTGCACCATGGTGTGATGGGTAATCCGCAAG
GCAACTCCGAGCCTGCTGCAACCACCCGTATTTATGACCAAAAAACCAAAACTTATGAATCTGATAACGCCTACGAAAAG
CGTATTTCAGATGATTTGAAGAAGAATATTGAAACCTTTAAGGCAAATGGAATTCGTGCACCGCGTGTGATGGTTTGGCC
GTATGGGCGATACAATCTTGAAACTGTTCGAATTGCCAAAAAATTGGGTATGCCAATTACCATTACACTTGATGATGGTC
CTGATCATGTCACCAAGTCTTTAGGTCATTTAAGCCGTATTTTGGTCAGTAAAGGTGAGAGTACCGATGATCTGGCGCAA
GACATTTTGACGCGTCAAATGAATTTGACAGACAATAATCGTCCGCAAAAAATTATGCATGTCGATCTGGATTATATTTA
TGATCCAAATCCAGAGCAGATCGAACGTAATTTGGGACACTTGTTGGATCGTATCCGCGAGATGGGTGTTAACACAGTAT
ATTTACAGGCTTTTTCAGATCCTGATGCCAATGGCTCTGCGGATATGGTGTATTTCCCGAATCGTTATATTCCAATGCGT
GCCGATTTATTTAACCGTGTCGCATGGCAAATTGCAACTCGCACCAGTGTCAACCGTGTATATGCATGGATGCCACTTAT
GGCTTGGGAACTACCAAAGAGCAATCTAGCGTCAAAAAAATTGGTGGTGACTCAACAGCCAAAAGAAAGCGATCATTTGA
ATATGGGATATATCCGTTTAAGTCCATATTCTCCTGAAGCACGTAAAGTGATTGAAGGAATTTACACAGATTTGGCGAAA
TCTTCGACATTTGATGGTATACTCTTCCACGATGATACCACCTTGTCTGATTATGAAGACGCAAGTCCAGATGCGCTGAA
GGCGTATGCAAAAGCGGGCTTGCCAACAGACCTCGAAAAAATCCGCAATGATGATGCTTTATTGTCTAAATGGACTGATT
TTAAAATTAAGACTATCGACAATTTTGCGATGAAGTTGGCCTCAGATGTTCGTTATTATCAACCGTATCTAATGACCGCA
CGTAATCTGTATGCGCAGGTTGCACTGTCTCCTTATGCAGAAAACTGGTATTCCCAGTCTCTTGAGCAGTCATTAAAACG
TTATGATTTCACTGCTATTATGGCCATGCCTTACATGGAACAAGCAGAAGATCCGACAAAGTTCTATCAGGATATTGTAA
AACGTGTCAAACAGTATCCAAATGGAATAAAGAAAACTGTATTTGAATTGCAGGCAACCAATTGGCGAACAAACCAACCT
GTTCCGTCTCAGGAGCTTGCCGATACAATTCGCTCTTTGTATCAACAAGGAGCTATGCATGTGGGCTATTATCCAGATGA
TCCATTTAAGGACCATCCTGATACCAAAACCATGCACGACGTATTTGTGACTAAACCTTCAAAGTTAGTCCCGTAA

Upstream 100 bases:

>100_bases
TGGACACTTTTATTTAAGTTTCAATCAAGGTCATGCTTAAAATCTTGCTTAAACTGAAAAAGTTTTCAGAAAACAACTTT
TTTTTAAAAGATGATATTTT

Downstream 100 bases:

>100_bases
CAGGAATTGACCATGTCAACCACTTTACATCATTTGTGGCAAACAGCGCTAAACTTCGTGTTTTATTACCCGCTGTTTAT
GTCCTATTTATGGATGATGG

Product: lipoprotein

Products: NA

Alternate protein names: PGA N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase [H]

Number of amino acids: Translated: 691; Mature: 691

Protein sequence:

>691_residues
MLTRFPLKLITLSVSTVLSLSLGCAHAETGTSTNDNPAVKEVPLVTNPHTGEEAEEAPKIDVSTLTIIGYHEITSKEAII
PDYAVTPQRFETHLDWLIKNGYHFISVDQLIKANEGKIKLPAKPVLLTVDDGYQSFYDYAYPILKARKIPVVLAVVGKWL
NVKPNQMVQFGDDKLARNKLLTWAEIKEMQDSGIVEIGSHSFDLHHGVMGNPQGNSEPAATTRIYDQKTKTYESDNAYEK
RISDDLKKNIETFKANGIRAPRVMVWPYGRYNLETVRIAKKLGMPITITLDDGPDHVTKSLGHLSRILVSKGESTDDLAQ
DILTRQMNLTDNNRPQKIMHVDLDYIYDPNPEQIERNLGHLLDRIREMGVNTVYLQAFSDPDANGSADMVYFPNRYIPMR
ADLFNRVAWQIATRTSVNRVYAWMPLMAWELPKSNLASKKLVVTQQPKESDHLNMGYIRLSPYSPEARKVIEGIYTDLAK
SSTFDGILFHDDTTLSDYEDASPDALKAYAKAGLPTDLEKIRNDDALLSKWTDFKIKTIDNFAMKLASDVRYYQPYLMTA
RNLYAQVALSPYAENWYSQSLEQSLKRYDFTAIMAMPYMEQAEDPTKFYQDIVKRVKQYPNGIKKTVFELQATNWRTNQP
VPSQELADTIRSLYQQGAMHVGYYPDDPFKDHPDTKTMHDVFVTKPSKLVP

Sequences:

>Translated_691_residues
MLTRFPLKLITLSVSTVLSLSLGCAHAETGTSTNDNPAVKEVPLVTNPHTGEEAEEAPKIDVSTLTIIGYHEITSKEAII
PDYAVTPQRFETHLDWLIKNGYHFISVDQLIKANEGKIKLPAKPVLLTVDDGYQSFYDYAYPILKARKIPVVLAVVGKWL
NVKPNQMVQFGDDKLARNKLLTWAEIKEMQDSGIVEIGSHSFDLHHGVMGNPQGNSEPAATTRIYDQKTKTYESDNAYEK
RISDDLKKNIETFKANGIRAPRVMVWPYGRYNLETVRIAKKLGMPITITLDDGPDHVTKSLGHLSRILVSKGESTDDLAQ
DILTRQMNLTDNNRPQKIMHVDLDYIYDPNPEQIERNLGHLLDRIREMGVNTVYLQAFSDPDANGSADMVYFPNRYIPMR
ADLFNRVAWQIATRTSVNRVYAWMPLMAWELPKSNLASKKLVVTQQPKESDHLNMGYIRLSPYSPEARKVIEGIYTDLAK
SSTFDGILFHDDTTLSDYEDASPDALKAYAKAGLPTDLEKIRNDDALLSKWTDFKIKTIDNFAMKLASDVRYYQPYLMTA
RNLYAQVALSPYAENWYSQSLEQSLKRYDFTAIMAMPYMEQAEDPTKFYQDIVKRVKQYPNGIKKTVFELQATNWRTNQP
VPSQELADTIRSLYQQGAMHVGYYPDDPFKDHPDTKTMHDVFVTKPSKLVP
>Mature_691_residues
MLTRFPLKLITLSVSTVLSLSLGCAHAETGTSTNDNPAVKEVPLVTNPHTGEEAEEAPKIDVSTLTIIGYHEITSKEAII
PDYAVTPQRFETHLDWLIKNGYHFISVDQLIKANEGKIKLPAKPVLLTVDDGYQSFYDYAYPILKARKIPVVLAVVGKWL
NVKPNQMVQFGDDKLARNKLLTWAEIKEMQDSGIVEIGSHSFDLHHGVMGNPQGNSEPAATTRIYDQKTKTYESDNAYEK
RISDDLKKNIETFKANGIRAPRVMVWPYGRYNLETVRIAKKLGMPITITLDDGPDHVTKSLGHLSRILVSKGESTDDLAQ
DILTRQMNLTDNNRPQKIMHVDLDYIYDPNPEQIERNLGHLLDRIREMGVNTVYLQAFSDPDANGSADMVYFPNRYIPMR
ADLFNRVAWQIATRTSVNRVYAWMPLMAWELPKSNLASKKLVVTQQPKESDHLNMGYIRLSPYSPEARKVIEGIYTDLAK
SSTFDGILFHDDTTLSDYEDASPDALKAYAKAGLPTDLEKIRNDDALLSKWTDFKIKTIDNFAMKLASDVRYYQPYLMTA
RNLYAQVALSPYAENWYSQSLEQSLKRYDFTAIMAMPYMEQAEDPTKFYQDIVKRVKQYPNGIKKTVFELQATNWRTNQP
VPSQELADTIRSLYQQGAMHVGYYPDDPFKDHPDTKTMHDVFVTKPSKLVP

Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PGA), a biofilm adhesin polysaccharide. N- deacetylation promotes PGA export through the PgaA porin [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Escherichia coli, GI1787260, Length=626, Percent_Identity=38.0191693290735, Blast_Score=451, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 78602; Mature: 78602

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRFPLKLITLSVSTVLSLSLGCAHAETGTSTNDNPAVKEVPLVTNPHTGEEAEEAPKI
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCEEECCCCCCCHHHCCCC
DVSTLTIIGYHEITSKEAIIPDYAVTPQRFETHLDWLIKNGYHFISVDQLIKANEGKIKL
EEEEEEEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCEEEC
PAKPVLLTVDDGYQSFYDYAYPILKARKIPVVLAVVGKWLNVKPNQMVQFGDDKLARNKL
CCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCHHEECCCHHHHHHHC
LTWAEIKEMQDSGIVEIGSHSFDLHHGVMGNPQGNSEPAATTRIYDQKTKTYESDNAYEK
CHHHHHHHHCCCCCEEECCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHH
RISDDLKKNIETFKANGIRAPRVMVWPYGRYNLETVRIAKKLGMPITITLDDGPDHVTKS
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHH
LGHLSRILVSKGESTDDLAQDILTRQMNLTDNNRPQKIMHVDLDYIYDPNPEQIERNLGH
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECEEECCCHHHHHHHHHH
LLDRIREMGVNTVYLQAFSDPDANGSADMVYFPNRYIPMRADLFNRVAWQIATRTSVNRV
HHHHHHHCCCCEEEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YAWMPLMAWELPKSNLASKKLVVTQQPKESDHLNMGYIRLSPYSPEARKVIEGIYTDLAK
HHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHC
SSTFDGILFHDDTTLSDYEDASPDALKAYAKAGLPTDLEKIRNDDALLSKWTDFKIKTID
CCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCHHHHHHCCCCEEEEHH
NFAMKLASDVRYYQPYLMTARNLYAQVALSPYAENWYSQSLEQSLKRYDFTAIMAMPYME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCHHHEEECCCHH
QAEDPTKFYQDIVKRVKQYPNGIKKTVFELQATNWRTNQPVPSQELADTIRSLYQQGAMH
HCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEE
VGYYPDDPFKDHPDTKTMHDVFVTKPSKLVP
ECCCCCCCCCCCCCCCCHHHHEEECCCCCCC
>Mature Secondary Structure
MLTRFPLKLITLSVSTVLSLSLGCAHAETGTSTNDNPAVKEVPLVTNPHTGEEAEEAPKI
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCEEECCCCCCCHHHCCCC
DVSTLTIIGYHEITSKEAIIPDYAVTPQRFETHLDWLIKNGYHFISVDQLIKANEGKIKL
EEEEEEEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCEEEC
PAKPVLLTVDDGYQSFYDYAYPILKARKIPVVLAVVGKWLNVKPNQMVQFGDDKLARNKL
CCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCHHEECCCHHHHHHHC
LTWAEIKEMQDSGIVEIGSHSFDLHHGVMGNPQGNSEPAATTRIYDQKTKTYESDNAYEK
CHHHHHHHHCCCCCEEECCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHH
RISDDLKKNIETFKANGIRAPRVMVWPYGRYNLETVRIAKKLGMPITITLDDGPDHVTKS
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHH
LGHLSRILVSKGESTDDLAQDILTRQMNLTDNNRPQKIMHVDLDYIYDPNPEQIERNLGH
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECEEECCCHHHHHHHHHH
LLDRIREMGVNTVYLQAFSDPDANGSADMVYFPNRYIPMRADLFNRVAWQIATRTSVNRV
HHHHHHHCCCCEEEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YAWMPLMAWELPKSNLASKKLVVTQQPKESDHLNMGYIRLSPYSPEARKVIEGIYTDLAK
HHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHC
SSTFDGILFHDDTTLSDYEDASPDALKAYAKAGLPTDLEKIRNDDALLSKWTDFKIKTID
CCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCHHHHHHCCCCEEEEHH
NFAMKLASDVRYYQPYLMTARNLYAQVALSPYAENWYSQSLEQSLKRYDFTAIMAMPYME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCHHHEEECCCHH
QAEDPTKFYQDIVKRVKQYPNGIKKTVFELQATNWRTNQPVPSQELADTIRSLYQQGAMH
HCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEE
VGYYPDDPFKDHPDTKTMHDVFVTKPSKLVP
ECCCCCCCCCCCCCCCCHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]