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The map label for this gene is pgaC [H]

Identifier: 50084232

GI number: 50084232

Start: 1022591

End: 1023865

Strand: Direct

Name: pgaC [H]

Synonym: ACIAD1030

Alternate gene names: 50084232

Gene position: 1022591-1023865 (Clockwise)

Preceding gene: 50084231

Following gene: 50084233

Centisome position: 28.42

GC content: 43.06

Gene sequence:

>1275_bases
TTGACCATGTCAACCACTTTACATCATTTGTGGCAAACAGCGCTAAACTTCGTGTTTTATTACCCGCTGTTTATGTCCTA
TTTATGGATGATGGGTGCGCTTATATTTTACTGGAAAGAGCGCAAAGATCCTCCATACAAAGAGCCAGCACCACTTGAAT
CATATCCAATGGTCGCTGTGCTTGTCCCTTGTTTTAATGAAGGGGATAATGCCGAGGAAACCATTACCCATGCACTGGGG
CTGGATTACCCAAATTTTGAGGTAATTGCCATTAATGACGGGAGTTCGGATAACACCGCAGACGTACTCAATCGTCTGGC
TGAAAAGTATGAAAAACTGCGTGTGGTTCATCTTGCTCAGAATCAGGGTAAGGCAATGGCGCTTCAAGCAGGCAGTCTGT
TAACTGAAGCAGAATTTTTAATTGGTATTGATGGTGATGCTTTACTCGACTCACACGCTGCAAAATGGATGATCAGACAT
TTTCAAGAAGATCATACCGTTGCGGCTGTGACGGGTAACCCACGTATTCGTACCCGTTCGACTTTGATTGGTCGTATTCA
GGTAGGTGAGTTTTCATCGATTGTGGGTATGATTAAACGTGCACAGCGTACTTTTGGGCGTTTGTTTACGGTTTCTGGTG
TCATTACGGCGTTTCGTAAAAGTGCTGTGCATGAGGTGAACTATTGGTCACCGAACATGCTTACCGAAGATATTGATATT
ACCTGGAAATTACAACGTGCTGGCTGGGATATCCGCTTTGAGCCAAATGCTCTGGTCTGGATTTTGATGCCTGAAACCTT
CCAAGGTTTATGGAAACAGCGTTTACGCTGGGCGATGGGTGGGGCACAGGTTTTAATTAAAAATCTGGATGTTTTCCTTA
AGCCAAAGTTAAATTTTTTATGGCCGTTAATGTTTGAATTATGCCTGACATTGGTATGGTCATATTTGATGTTGATCATG
GCTGTATTGTGGTTGATACATTTTGTGTTCCCACTTGGGCAGCTTTCAGGAATCAGTTCACCGTTTTTACCTTACGGTAG
TGGAATTTTGTTGGGTGCTACCTGTCTGATCCAATTTGCAATGAGTAAATGGATGGATAGCCGTTATGAACCAAACCTCG
GTAAAAACTATTACTGGATGATCTGGTATCCGTTTGTGTTTTGGTTAATTACCATCACAGCGACAATCATGGCTTTTCCA
AAAGTTCTGCTGCGTGGTAAAGAAAAACGTGCGCGTTGGGTGAGCCCTGACCGTGGTATTCGTATTCAAGATTAA

Upstream 100 bases:

>100_bases
GGGCTATTATCCAGATGATCCATTTAAGGACCATCCTGATACCAAAACCATGCACGACGTATTTGTGACTAAACCTTCAA
AGTTAGTCCCGTAACAGGAA

Downstream 100 bases:

>100_bases
GCATATTTGCTGAGTAGATGTTCATGAAGTCACATGATTTGATTATCGATTTGCGTCGCCAATTACCTTGGCATAAACGT
TATGTTTCAATGACAACGAC

Product: N-glycosyl transferase

Products: NA

Alternate protein names: PGA synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm PGA synthesis protein PgaC; N-acetylglucosaminyltransferase PgaC [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTMSTTLHHLWQTALNFVFYYPLFMSYLWMMGALIFYWKERKDPPYKEPAPLESYPMVAVLVPCFNEGDNAEETITHALG
LDYPNFEVIAINDGSSDNTADVLNRLAEKYEKLRVVHLAQNQGKAMALQAGSLLTEAEFLIGIDGDALLDSHAAKWMIRH
FQEDHTVAAVTGNPRIRTRSTLIGRIQVGEFSSIVGMIKRAQRTFGRLFTVSGVITAFRKSAVHEVNYWSPNMLTEDIDI
TWKLQRAGWDIRFEPNALVWILMPETFQGLWKQRLRWAMGGAQVLIKNLDVFLKPKLNFLWPLMFELCLTLVWSYLMLIM
AVLWLIHFVFPLGQLSGISSPFLPYGSGILLGATCLIQFAMSKWMDSRYEPNLGKNYYWMIWYPFVFWLITITATIMAFP
KVLLRGKEKRARWVSPDRGIRIQD

Sequences:

>Translated_424_residues
MTMSTTLHHLWQTALNFVFYYPLFMSYLWMMGALIFYWKERKDPPYKEPAPLESYPMVAVLVPCFNEGDNAEETITHALG
LDYPNFEVIAINDGSSDNTADVLNRLAEKYEKLRVVHLAQNQGKAMALQAGSLLTEAEFLIGIDGDALLDSHAAKWMIRH
FQEDHTVAAVTGNPRIRTRSTLIGRIQVGEFSSIVGMIKRAQRTFGRLFTVSGVITAFRKSAVHEVNYWSPNMLTEDIDI
TWKLQRAGWDIRFEPNALVWILMPETFQGLWKQRLRWAMGGAQVLIKNLDVFLKPKLNFLWPLMFELCLTLVWSYLMLIM
AVLWLIHFVFPLGQLSGISSPFLPYGSGILLGATCLIQFAMSKWMDSRYEPNLGKNYYWMIWYPFVFWLITITATIMAFP
KVLLRGKEKRARWVSPDRGIRIQD
>Mature_423_residues
TMSTTLHHLWQTALNFVFYYPLFMSYLWMMGALIFYWKERKDPPYKEPAPLESYPMVAVLVPCFNEGDNAEETITHALGL
DYPNFEVIAINDGSSDNTADVLNRLAEKYEKLRVVHLAQNQGKAMALQAGSLLTEAEFLIGIDGDALLDSHAAKWMIRHF
QEDHTVAAVTGNPRIRTRSTLIGRIQVGEFSSIVGMIKRAQRTFGRLFTVSGVITAFRKSAVHEVNYWSPNMLTEDIDIT
WKLQRAGWDIRFEPNALVWILMPETFQGLWKQRLRWAMGGAQVLIKNLDVFLKPKLNFLWPLMFELCLTLVWSYLMLIMA
VLWLIHFVFPLGQLSGISSPFLPYGSGILLGATCLIQFAMSKWMDSRYEPNLGKNYYWMIWYPFVFWLITITATIMAFPK
VLLRGKEKRARWVSPDRGIRIQD

Specific function: Probable N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N- acetylglucosamine to produce the linear homopolymer poly-beta-1,6- N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide [H]

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=423, Percent_Identity=49.4089834515366, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1786560, Length=214, Percent_Identity=25.2336448598131, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 48899; Mature: 48768

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMSTTLHHLWQTALNFVFYYPLFMSYLWMMGALIFYWKERKDPPYKEPAPLESYPMVAV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
LVPCFNEGDNAEETITHALGLDYPNFEVIAINDGSSDNTADVLNRLAEKYEKLRVVHLAQ
EEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEEC
NQGKAMALQAGSLLTEAEFLIGIDGDALLDSHAAKWMIRHFQEDHTVAAVTGNPRIRTRS
CCCCEEEEECCCHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEHH
TLIGRIQVGEFSSIVGMIKRAQRTFGRLFTVSGVITAFRKSAVHEVNYWSPNMLTEDIDI
HEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCE
TWKLQRAGWDIRFEPNALVWILMPETFQGLWKQRLRWAMGGAQVLIKNLDVFLKPKLNFL
EEEEECCCCEEEECCCEEEEEECCHHHHHHHHHHHHHHHCHHHHHHHHCCEEECCCHHHH
WPLMFELCLTLVWSYLMLIMAVLWLIHFVFPLGQLSGISSPFLPYGSGILLGATCLIQFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
MSKWMDSRYEPNLGKNYYWMIWYPFVFWLITITATIMAFPKVLLRGKEKRARWVSPDRGI
HHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCC
RIQD
EECC
>Mature Secondary Structure 
TMSTTLHHLWQTALNFVFYYPLFMSYLWMMGALIFYWKERKDPPYKEPAPLESYPMVAV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
LVPCFNEGDNAEETITHALGLDYPNFEVIAINDGSSDNTADVLNRLAEKYEKLRVVHLAQ
EEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEEC
NQGKAMALQAGSLLTEAEFLIGIDGDALLDSHAAKWMIRHFQEDHTVAAVTGNPRIRTRS
CCCCEEEEECCCHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEHH
TLIGRIQVGEFSSIVGMIKRAQRTFGRLFTVSGVITAFRKSAVHEVNYWSPNMLTEDIDI
HEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCE
TWKLQRAGWDIRFEPNALVWILMPETFQGLWKQRLRWAMGGAQVLIKNLDVFLKPKLNFL
EEEEECCCCEEEECCCEEEEEECCHHHHHHHHHHHHHHHCHHHHHHHHCCEEECCCHHHH
WPLMFELCLTLVWSYLMLIMAVLWLIHFVFPLGQLSGISSPFLPYGSGILLGATCLIQFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
MSKWMDSRYEPNLGKNYYWMIWYPFVFWLITITATIMAFPKVLLRGKEKRARWVSPDRGI
HHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCC
RIQD
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]