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The map label for this gene is dctA [H]

Identifier: 50084228

GI number: 50084228

Start: 1017738

End: 1018868

Strand: Reverse

Name: dctA [H]

Synonym: ACIAD1026

Alternate gene names: 50084228

Gene position: 1018868-1017738 (Counterclockwise)

Preceding gene: 50084238

Following gene: 50084226

Centisome position: 28.31

GC content: 39.17

Gene sequence:

>1131_bases
TTGATTGCACCCATCGTTTTTTGTGTTGTAGTTCTTGGAATTTATGGTGCAAATGATATTAAAAAAATGGGTAAAGTTGG
TGCAAAAACCATTCTCTATTTTGAAGCAGTCACTTCAATTGCACTTATTTTGGGCATTGCAATTGCGTATATATTCAAAC
CAGGTGTAGGAATGAATATTGACATTAGCAAACTCGATGGTAAGGACTTAACCACATATACCGATCGTGCTCATGAGATG
AGTACGGGTTCAGACTTTATTTTACATATCATTCCTAAGACTTTGGTGAGTGCATTTGCCAATGGCGATATTTTACAAGT
TCTGCTCATTGCAATTTTGTTTGGCGTCGCCATGCTGCTGGTCCCCAAACATCTTGCTGAAATTGTCCGCCAAGCACTGG
AAGCATTTTCAGAAATTTTCTTCAAAATGATGGGAATGATCATTCGTCTTGCACCACTCGGTGTTTTAGGCTCGGTGGCT
TATACCACCGCCAAATTTGGTGCGGATTCATTAATTCAACTGGGTTATCTGGTTTTACTTTTCTACATCACCTGTATTGT
ATTTGTGGTGGTCATTTTAGGTGGCATCCTGAAAATGGCAGGACTGAATATCTTTAAATTTCTGAACTATTTTAAAGATG
AATTGTCAATTGTACTGGGTACGGCCTCTTCTGATTCTGTGCTTCCACAAGTCATGAAAAAACTCAAACAGTTAGGTATT
AAGGATTCAACCGTAGGTCTGGTCATCCCGACAGGTTATTCATTTAATCTGGATGGTTTTTCAATCTATTTAACCTTAGG
TGTTATTTTTATTGCCCAAGCCTGCAATATTGATTTATCCATGCATAATCTTTTGATGATTCTTCTAGTTTCTTTGGTGA
CTTCAAAAGGTGCGCATGGAATTCCAGGTTCTGCTTTGGTAATTCTTGCTGCAACGTTATCAGTCATTCCAAGCCTACCT
GCAATTGGTTTAGTCCTTTTACTCTCTGTAGACTGGTTTATTGGGATTGTGAGAGCGTGTACAAACCTCATTGGTAATTG
TGTTGCCACGGTAGTGATTGCACATTGGGAAAAGGACATTGACCATGCCCAAGCACGTGCCGTACTTGAGCATAAATCAT
CAGTCAACTAA

Upstream 100 bases:

>100_bases
TATCGCCTTAATTGTTGGGATGATCATAGGTATCAGCTTTCATGATTTTTCTTTAAATTTAAAGCCTTTAGGTGATGGCT
TTATTTCTCTCATTAAAATG

Downstream 100 bases:

>100_bases
ACACTTAAGTATTCTGCTCATCTAGCCGACATGCCATTAACTGTTTCTCAAAATCAAGCCAGTTGCCTAACTGGCTTGTA
ATAATTTCAATTCGACTATC

Product: aerobic C4-dicarboxylate transport protein

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MIAPIVFCVVVLGIYGANDIKKMGKVGAKTILYFEAVTSIALILGIAIAYIFKPGVGMNIDISKLDGKDLTTYTDRAHEM
STGSDFILHIIPKTLVSAFANGDILQVLLIAILFGVAMLLVPKHLAEIVRQALEAFSEIFFKMMGMIIRLAPLGVLGSVA
YTTAKFGADSLIQLGYLVLLFYITCIVFVVVILGGILKMAGLNIFKFLNYFKDELSIVLGTASSDSVLPQVMKKLKQLGI
KDSTVGLVIPTGYSFNLDGFSIYLTLGVIFIAQACNIDLSMHNLLMILLVSLVTSKGAHGIPGSALVILAATLSVIPSLP
AIGLVLLLSVDWFIGIVRACTNLIGNCVATVVIAHWEKDIDHAQARAVLEHKSSVN

Sequences:

>Translated_376_residues
MIAPIVFCVVVLGIYGANDIKKMGKVGAKTILYFEAVTSIALILGIAIAYIFKPGVGMNIDISKLDGKDLTTYTDRAHEM
STGSDFILHIIPKTLVSAFANGDILQVLLIAILFGVAMLLVPKHLAEIVRQALEAFSEIFFKMMGMIIRLAPLGVLGSVA
YTTAKFGADSLIQLGYLVLLFYITCIVFVVVILGGILKMAGLNIFKFLNYFKDELSIVLGTASSDSVLPQVMKKLKQLGI
KDSTVGLVIPTGYSFNLDGFSIYLTLGVIFIAQACNIDLSMHNLLMILLVSLVTSKGAHGIPGSALVILAATLSVIPSLP
AIGLVLLLSVDWFIGIVRACTNLIGNCVATVVIAHWEKDIDHAQARAVLEHKSSVN
>Mature_376_residues
MIAPIVFCVVVLGIYGANDIKKMGKVGAKTILYFEAVTSIALILGIAIAYIFKPGVGMNIDISKLDGKDLTTYTDRAHEM
STGSDFILHIIPKTLVSAFANGDILQVLLIAILFGVAMLLVPKHLAEIVRQALEAFSEIFFKMMGMIIRLAPLGVLGSVA
YTTAKFGADSLIQLGYLVLLFYITCIVFVVVILGGILKMAGLNIFKFLNYFKDELSIVLGTASSDSVLPQVMKKLKQLGI
KDSTVGLVIPTGYSFNLDGFSIYLTLGVIFIAQACNIDLSMHNLLMILLVSLVTSKGAHGIPGSALVILAATLSVIPSLP
AIGLVLLLSVDWFIGIVRACTNLIGNCVATVVIAHWEKDIDHAQARAVLEHKSSVN

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI4827012, Length=329, Percent_Identity=27.355623100304, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI169790839, Length=431, Percent_Identity=24.1299303944316, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI5032093, Length=406, Percent_Identity=24.384236453202, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI40254478, Length=430, Percent_Identity=23.7209302325581, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI21314632, Length=416, Percent_Identity=23.7980769230769, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI66773030, Length=384, Percent_Identity=23.9583333333333, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI223468566, Length=280, Percent_Identity=26.7857142857143, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI223468564, Length=280, Percent_Identity=26.7857142857143, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI194239697, Length=159, Percent_Identity=32.0754716981132, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI301601644, Length=191, Percent_Identity=25.130890052356, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1789947, Length=373, Percent_Identity=48.2573726541555, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1790514, Length=371, Percent_Identity=38.544474393531, Blast_Score=270, Evalue=9e-74,
Organism=Escherichia coli, GI1788024, Length=377, Percent_Identity=23.0769230769231, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193206654, Length=418, Percent_Identity=24.6411483253589, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17537407, Length=418, Percent_Identity=24.6411483253589, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI193206505, Length=404, Percent_Identity=25.990099009901, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI71996953, Length=398, Percent_Identity=25.1256281407035, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71983099, Length=431, Percent_Identity=22.969837587007, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17541374, Length=157, Percent_Identity=33.7579617834395, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983106, Length=175, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24583025, Length=402, Percent_Identity=22.3880597014925, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137668, Length=402, Percent_Identity=22.3880597014925, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583023, Length=402, Percent_Identity=22.3880597014925, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281360481, Length=416, Percent_Identity=23.7980769230769, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI281360483, Length=416, Percent_Identity=23.7980769230769, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17137666, Length=416, Percent_Identity=23.7980769230769, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 40329; Mature: 40329

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAPIVFCVVVLGIYGANDIKKMGKVGAKTILYFEAVTSIALILGIAIAYIFKPGVGMNI
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
DISKLDGKDLTTYTDRAHEMSTGSDFILHIIPKTLVSAFANGDILQVLLIAILFGVAMLL
EEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VPKHLAEIVRQALEAFSEIFFKMMGMIIRLAPLGVLGSVAYTTAKFGADSLIQLGYLVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
FYITCIVFVVVILGGILKMAGLNIFKFLNYFKDELSIVLGTASSDSVLPQVMKKLKQLGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHCCC
KDSTVGLVIPTGYSFNLDGFSIYLTLGVIFIAQACNIDLSMHNLLMILLVSLVTSKGAHG
CCCCCEEEEECCCEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
IPGSALVILAATLSVIPSLPAIGLVLLLSVDWFIGIVRACTNLIGNCVATVVIAHWEKDI
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHAQARAVLEHKSSVN
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIAPIVFCVVVLGIYGANDIKKMGKVGAKTILYFEAVTSIALILGIAIAYIFKPGVGMNI
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
DISKLDGKDLTTYTDRAHEMSTGSDFILHIIPKTLVSAFANGDILQVLLIAILFGVAMLL
EEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VPKHLAEIVRQALEAFSEIFFKMMGMIIRLAPLGVLGSVAYTTAKFGADSLIQLGYLVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
FYITCIVFVVVILGGILKMAGLNIFKFLNYFKDELSIVLGTASSDSVLPQVMKKLKQLGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHCCC
KDSTVGLVIPTGYSFNLDGFSIYLTLGVIFIAQACNIDLSMHNLLMILLVSLVTSKGAHG
CCCCCEEEEECCCEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
IPGSALVILAATLSVIPSLPAIGLVLLLSVDWFIGIVRACTNLIGNCVATVVIAHWEKDI
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHAQARAVLEHKSSVN
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA