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The map label for this gene is shiA [H]

Identifier: 50084238

GI number: 50084238

Start: 1027247

End: 1028542

Strand: Reverse

Name: shiA [H]

Synonym: ACIAD1036

Alternate gene names: 50084238

Gene position: 1028542-1027247 (Counterclockwise)

Preceding gene: 50084239

Following gene: 50084228

Centisome position: 28.58

GC content: 41.82

Gene sequence:

>1296_bases
ATGGATGCCGTCCCTCAGCTTTCCGCACAAGATAAACGTACTCTTGCACTTTCATCATTAGGCGGAGCATTAGAATTTTA
CGACTTTGTCATCTATGTTTTTTACGCCAAAATTATTTCAGACTTATTTTTTCCAAGTACTTTAAGTCCCTTTTGGGCGA
TGCTCAATACATATGGCATTTTTGCAGCAGGATATTTTTTTCGTCCACTGGGTGGCATCGTCATGGCACATTTTGGCGAT
CTGGTTGGACGCAAACGACTATTTTCACTTTCAATTTTATTGATGTCATTGCCCACACTTATGATTGGGATTTTGCCCAC
CTTTGAAACCATCGGTTATGCTGCTCCCCTGCTTTTGCTGATGATGCGGATCATTCAGGGTATTGCGATTGGCGGTGAGA
TTCCTGCTGCATGGACCTTTGTGTCTGAGCATGTTCCTGAGTCACGTATTGGAATTGCCAATGGACTATTAACCGCAGGC
TTATCTTTAGGAATTCTGCTGGGCGCACTCATGTCGCTGTTAATCTCTGTTCAATTTACTGAACCTGACATACATGCCTG
GGCATGGCGTATTGCTTTTGTGGCAGGTGGTATTTTTGGTCTATTTGCGCTTTATTTAAGAAGCTATCTGAAAGAAACTC
CTGTATTTAAAACCATGCAAGCACGCAAACAATTGTCAAAAGAACTGCCAGTGCAACAGGTTGTTATGCATCATCGTTCG
GCTGTTGTGATTGGAATGCTATTTACCTGGTTTCTCACTGGATGCGTCGTGGTGATTATTCTGGCAATGCCGAATTTATT
AATTGGTACTTTTGGCTACGAACGTGCGCTTACCTTTAAAATGCAAAGTGCAGCAATTCTGATGCAAATGCTCGGGTGTA
TATTGGCAGGTACACTGGCAGACAGATTTGGCTGTGCCAGAGTTATGATGGCTGGTGCGACAACACTTGCCGTCACTGCT
GGGATTTTTTATCAAAGCCTTGAGCATGCAAGTCATAGCACTGTCTTTATTTTATATATGTTGCTTGGATTATGCTCGGG
AACAGTAGGTATGGTGTCATGCAGTATGGTTAAAATGTTTCCTGCCCCTATTCGCTTTTCTGGCATTTCTTTTTCTTATA
ATGTGTCTTATGCGATTGTGGGTGGTATGACTCTGCCTTTAATTCAGATTCTGAGCCAATACAGCAAAATTGGCGCCATG
TATTATATTTTTGCGCTGGCTATTATGGCTGTATGTACAGCCTTTGTGTTTAAAACAAAATTTGAGACCGCACAAAAATA
TTCAGTAGATACATAA

Upstream 100 bases:

>100_bases
CAATATGGCGTGGCCTACCGTAAATAGAATGAATCAATTTACTTTTAATTTAAAATTCTATTAATATCAAACACACTTTT
TTAATGATTATTTATACAGC

Downstream 100 bases:

>100_bases
ATAAAAAGCGCGTATTTTTAAAGAGTACGCGCTTTTTATTTTAAAGTGCCTGAAGAAACTCCAAGCCCTTTTTAAGTTCG
AGCTCATTTACAGAGTGGCC

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MDAVPQLSAQDKRTLALSSLGGALEFYDFVIYVFYAKIISDLFFPSTLSPFWAMLNTYGIFAAGYFFRPLGGIVMAHFGD
LVGRKRLFSLSILLMSLPTLMIGILPTFETIGYAAPLLLLMMRIIQGIAIGGEIPAAWTFVSEHVPESRIGIANGLLTAG
LSLGILLGALMSLLISVQFTEPDIHAWAWRIAFVAGGIFGLFALYLRSYLKETPVFKTMQARKQLSKELPVQQVVMHHRS
AVVIGMLFTWFLTGCVVVIILAMPNLLIGTFGYERALTFKMQSAAILMQMLGCILAGTLADRFGCARVMMAGATTLAVTA
GIFYQSLEHASHSTVFILYMLLGLCSGTVGMVSCSMVKMFPAPIRFSGISFSYNVSYAIVGGMTLPLIQILSQYSKIGAM
YYIFALAIMAVCTAFVFKTKFETAQKYSVDT

Sequences:

>Translated_431_residues
MDAVPQLSAQDKRTLALSSLGGALEFYDFVIYVFYAKIISDLFFPSTLSPFWAMLNTYGIFAAGYFFRPLGGIVMAHFGD
LVGRKRLFSLSILLMSLPTLMIGILPTFETIGYAAPLLLLMMRIIQGIAIGGEIPAAWTFVSEHVPESRIGIANGLLTAG
LSLGILLGALMSLLISVQFTEPDIHAWAWRIAFVAGGIFGLFALYLRSYLKETPVFKTMQARKQLSKELPVQQVVMHHRS
AVVIGMLFTWFLTGCVVVIILAMPNLLIGTFGYERALTFKMQSAAILMQMLGCILAGTLADRFGCARVMMAGATTLAVTA
GIFYQSLEHASHSTVFILYMLLGLCSGTVGMVSCSMVKMFPAPIRFSGISFSYNVSYAIVGGMTLPLIQILSQYSKIGAM
YYIFALAIMAVCTAFVFKTKFETAQKYSVDT
>Mature_431_residues
MDAVPQLSAQDKRTLALSSLGGALEFYDFVIYVFYAKIISDLFFPSTLSPFWAMLNTYGIFAAGYFFRPLGGIVMAHFGD
LVGRKRLFSLSILLMSLPTLMIGILPTFETIGYAAPLLLLMMRIIQGIAIGGEIPAAWTFVSEHVPESRIGIANGLLTAG
LSLGILLGALMSLLISVQFTEPDIHAWAWRIAFVAGGIFGLFALYLRSYLKETPVFKTMQARKQLSKELPVQQVVMHHRS
AVVIGMLFTWFLTGCVVVIILAMPNLLIGTFGYERALTFKMQSAAILMQMLGCILAGTLADRFGCARVMMAGATTLAVTA
GIFYQSLEHASHSTVFILYMLLGLCSGTVGMVSCSMVKMFPAPIRFSGISFSYNVSYAIVGGMTLPLIQILSQYSKIGAM
YYIFALAIMAVCTAFVFKTKFETAQKYSVDT

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=389, Percent_Identity=32.6478149100257, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1790550, Length=428, Percent_Identity=29.2056074766355, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI1788942, Length=389, Percent_Identity=30.0771208226221, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789941, Length=388, Percent_Identity=30.4123711340206, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI87082404, Length=417, Percent_Identity=24.4604316546763, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87082231, Length=268, Percent_Identity=25, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47124; Mature: 47124

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCCCC
>Mature Secondary Structure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HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]