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The map label for this gene is acoA [H]

Identifier: 50084219

GI number: 50084219

Start: 1008126

End: 1009088

Strand: Direct

Name: acoA [H]

Synonym: ACIAD1017

Alternate gene names: 50084219

Gene position: 1008126-1009088 (Clockwise)

Preceding gene: 50084216

Following gene: 50084220

Centisome position: 28.01

GC content: 43.61

Gene sequence:

>963_bases
ATGCAATTAACGGAAGAGCAATTATTAGCCGCATATAAACGGATGCGAGATATTCGTGAATTTGAAGACCGTCTTCATGA
AGAAAATAATACAGGTGATATTCCTGGATTTATTCATCTATACAGTGGTGAAGAGGCGGTTGCGGTAGGCGTCTGTGAGA
ATCTCACCGATCAGGATTACATTACCTCTACCCATCGTGGGCATGGGCATTGTATTGCGAAAGGCTGCGATATTCACGAC
ATGATGCTGGAAATTTTTGGAAAAGATGCAGGATTGTGCCGAGGTAAAGGTGGATCGATGCATATTGCCGATTTGGATAA
AGGAATGCTGGGAGCCAATGGGATTGTAGGAGGTGGGCCACCGCTTGCAATTGGGGCAGCTTTAACGGCAAAAACATTAA
AAACTGGTGGTATTGGCTTGTCTTTTACAGGTGATGGTGGATCGAATCAGGGCCTGACTTTTGAAGCGATCAATATGGCA
GTTGTTTTGAAATTGCCTGTAATTTTTGTTTTCGAAAACAATGGTTTTGGTGAAGGTACGGCACATGATTATGCAGTCGG
AAGTAAGGACATTGCAGGGCGTGCTGCAGGCTTTGGTTTACCTGCCGAGAAGGTAGATGGCACAGATTTTTTTGCAGTTT
ACGCTGCTGCACAAAAAGCAATACAACGTGCACGTCGTGGTGAAGGGCCAAGTGTGATTGAAACAGTCACCAATCGTTTT
TATGGACATTTTGAAGGCGACCCAGGTCTCATCCGTTCTAAAGATGAAATTGATTATGTGAAGGAACATAAAGATCCGAT
CAAGATTTTCCGTGAAAAGGTCAAGGGCCAGATTGATGAAGCCAAATTGGATGCCATTGATGCTGTTTCAAAAGCCAATG
TCGATGATGCTGTAGCCAAAGCACGAGCAGCAAAATATCCCGAAGTCTCTCAACTTTTGACAGATGTCTATGTGTCTTAC
TAA

Upstream 100 bases:

>100_bases
AATAGAAAAGAAAATAAAAAAGGATTTATCTATTTGTATGAGTCATCTGCTCATACAACTTGGTTTCACTATTTTAAATC
AAGATAAATAGGATAAGGAT

Downstream 100 bases:

>100_bases
AGGAATAGTAAAGAAATGCCAAATAAAAGCTATCGAAATGCCATTAAAGAAGCCATTGAGCTAGAAATGCGCCGTGATCC
GACTGTCATTGTGGTGGGTG

Product: acetoin:2,6-dichlorophenolindophenol oxidoreductase alpha subunit

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MQLTEEQLLAAYKRMRDIREFEDRLHEENNTGDIPGFIHLYSGEEAVAVGVCENLTDQDYITSTHRGHGHCIAKGCDIHD
MMLEIFGKDAGLCRGKGGSMHIADLDKGMLGANGIVGGGPPLAIGAALTAKTLKTGGIGLSFTGDGGSNQGLTFEAINMA
VVLKLPVIFVFENNGFGEGTAHDYAVGSKDIAGRAAGFGLPAEKVDGTDFFAVYAAAQKAIQRARRGEGPSVIETVTNRF
YGHFEGDPGLIRSKDEIDYVKEHKDPIKIFREKVKGQIDEAKLDAIDAVSKANVDDAVAKARAAKYPEVSQLLTDVYVSY

Sequences:

>Translated_320_residues
MQLTEEQLLAAYKRMRDIREFEDRLHEENNTGDIPGFIHLYSGEEAVAVGVCENLTDQDYITSTHRGHGHCIAKGCDIHD
MMLEIFGKDAGLCRGKGGSMHIADLDKGMLGANGIVGGGPPLAIGAALTAKTLKTGGIGLSFTGDGGSNQGLTFEAINMA
VVLKLPVIFVFENNGFGEGTAHDYAVGSKDIAGRAAGFGLPAEKVDGTDFFAVYAAAQKAIQRARRGEGPSVIETVTNRF
YGHFEGDPGLIRSKDEIDYVKEHKDPIKIFREKVKGQIDEAKLDAIDAVSKANVDDAVAKARAAKYPEVSQLLTDVYVSY
>Mature_320_residues
MQLTEEQLLAAYKRMRDIREFEDRLHEENNTGDIPGFIHLYSGEEAVAVGVCENLTDQDYITSTHRGHGHCIAKGCDIHD
MMLEIFGKDAGLCRGKGGSMHIADLDKGMLGANGIVGGGPPLAIGAALTAKTLKTGGIGLSFTGDGGSNQGLTFEAINMA
VVLKLPVIFVFENNGFGEGTAHDYAVGSKDIAGRAAGFGLPAEKVDGTDFFAVYAAAQKAIQRARRGEGPSVIETVTNRF
YGHFEGDPGLIRSKDEIDYVKEHKDPIKIFREKVKGQIDEAKLDAIDAVSKANVDDAVAKARAAKYPEVSQLLTDVYVSY

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=320, Percent_Identity=35.9375, Blast_Score=192, Evalue=2e-49,
Organism=Homo sapiens, GI4505685, Length=320, Percent_Identity=35.3125, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI291084742, Length=320, Percent_Identity=35.3125, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI291084744, Length=327, Percent_Identity=34.5565749235474, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI291084757, Length=320, Percent_Identity=31.875, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI11386135, Length=318, Percent_Identity=28.3018867924528, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI258645172, Length=318, Percent_Identity=28.3018867924528, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32564172, Length=318, Percent_Identity=37.4213836477987, Blast_Score=196, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17536047, Length=318, Percent_Identity=37.4213836477987, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI86563355, Length=317, Percent_Identity=28.7066246056782, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI86563357, Length=317, Percent_Identity=28.7066246056782, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321026, Length=322, Percent_Identity=37.5776397515528, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24639744, Length=320, Percent_Identity=41.5625, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI28571106, Length=320, Percent_Identity=41.5625, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24639740, Length=320, Percent_Identity=41.5625, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24639748, Length=320, Percent_Identity=38.125, Blast_Score=201, Evalue=8e-52,
Organism=Drosophila melanogaster, GI24639746, Length=306, Percent_Identity=41.8300653594771, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI21355903, Length=321, Percent_Identity=26.791277258567, Blast_Score=94, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 34354; Mature: 34354

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLTEEQLLAAYKRMRDIREFEDRLHEENNTGDIPGFIHLYSGEEAVAVGVCENLTDQDY
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEHHHCCCCCHH
ITSTHRGHGHCIAKGCDIHDMMLEIFGKDAGLCRGKGGSMHIADLDKGMLGANGIVGGGP
HHCCCCCCCEEEECCCCHHHHHHHHHCCCCCCEECCCCCEEEEECCCCCCCCCCCCCCCC
PLAIGAALTAKTLKTGGIGLSFTGDGGSNQGLTFEAINMAVVLKLPVIFVFENNGFGEGT
CHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHCEEEEEECCEEEEECCCCCCCCC
AHDYAVGSKDIAGRAAGFGLPAEKVDGTDFFAVYAAAQKAIQRARRGEGPSVIETVTNRF
CCCCCCCCCHHCCCHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
YGHFEGDPGLIRSKDEIDYVKEHKDPIKIFREKVKGQIDEAKLDAIDAVSKANVDDAVAK
CEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHH
ARAAKYPEVSQLLTDVYVSY
HHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQLTEEQLLAAYKRMRDIREFEDRLHEENNTGDIPGFIHLYSGEEAVAVGVCENLTDQDY
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEHHHCCCCCHH
ITSTHRGHGHCIAKGCDIHDMMLEIFGKDAGLCRGKGGSMHIADLDKGMLGANGIVGGGP
HHCCCCCCCEEEECCCCHHHHHHHHHCCCCCCEECCCCCEEEEECCCCCCCCCCCCCCCC
PLAIGAALTAKTLKTGGIGLSFTGDGGSNQGLTFEAINMAVVLKLPVIFVFENNGFGEGT
CHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHCEEEEEECCEEEEECCCCCCCCC
AHDYAVGSKDIAGRAAGFGLPAEKVDGTDFFAVYAAAQKAIQRARRGEGPSVIETVTNRF
CCCCCCCCCHHCCCHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
YGHFEGDPGLIRSKDEIDYVKEHKDPIKIFREKVKGQIDEAKLDAIDAVSKANVDDAVAK
CEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHH
ARAAKYPEVSQLLTDVYVSY
HHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]