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The map label for this gene is acoB [H]

Identifier: 50084220

GI number: 50084220

Start: 1009104

End: 1010123

Strand: Direct

Name: acoB [H]

Synonym: ACIAD1018

Alternate gene names: 50084220

Gene position: 1009104-1010123 (Clockwise)

Preceding gene: 50084219

Following gene: 50084221

Centisome position: 28.04

GC content: 46.08

Gene sequence:

>1020_bases
ATGCCAAATAAAAGCTATCGAAATGCCATTAAAGAAGCCATTGAGCTAGAAATGCGCCGTGATCCGACTGTCATTGTGGT
GGGTGAAGATGTCCGTGGCGGGCATGGTGGCAAAAATACAGATGAAAATAAGCTCGAAGGTTTTGGTGGTGTATTGGGTG
TAACCAAAGGTTTGTGGACTGAATTCGGTTCTGAACGTGTGATTGATACCCCTATTACCGAGTCTGCCATTATTGGAATG
ACCGCAGGCGCGGCAGCAACAGGCTTACGTCCAGTTGCAGATTTGATGTTTATGGATTTTTATGGTGTCTGTCACGACAT
GCTCTATAACCAGGCCGCCAAATTCCGTTATATGTTTGGTGGTAAAGCCAAAGCACCGATGGTCGTGAGAGGCATGATTG
GTGCTGGTTTTTCGGCTGCAGCTCAACATTCCCAGTCACCTTATAATGTCTTTGCATCTGTACCTGGTTTAAAGGTCGTG
GTGCCATCTAGTCCTTATGATGTCAAGGGTTTACTGATTCAAGCCATTCGAGATAACGACCCTGTTGTGTTTTGTGAACA
TAAACTCTTGTATGACATCAAGGGCGAAGCACCAGATGAAGCCTATACTATTCCGTTTGGGGTTGCCAATTACACCCGTG
AAGGAACCGATGTCACCATTATTGCATTGAGCCTCATGGTTCATCGGGCCAATGAAGTTGCAGACAAACTCGCCAAAGAA
GGTATCTCTGTGGAGGTGGTCGATCCACGTACTGTTTCACCACTTGATGAAGATGGGATTTTAGAATCCGTTGCAAGCAC
GGGTCGTGTGGTGATTGTGGATGAATCAGCAGCACGATGCGGTTTTGGACATGATGTTGCGGCGCTCATTGCACAAAAGG
GTTTTCACTATTTAAAAGCACCTATCGAACTGGTTACACCGCCACATACGCCTATTCCTTTTTCACCAGTACTTGAAAAA
GAATGGATACCAAGTGTCGAGCGGATCGAACAAGCGGTCAGAAAAATACTGGAGGCTTAG

Upstream 100 bases:

>100_bases
TGTCGATGATGCTGTAGCCAAAGCACGAGCAGCAAAATATCCCGAAGTCTCTCAACTTTTGACAGATGTCTATGTGTCTT
ACTAAAGGAATAGTAAAGAA

Downstream 100 bases:

>100_bases
GATGAGCGAAATTAAAACACTGGAAATCCCGAAATGGGGATTGTCCATGGAAGAAGGAACAGTCGCACAGTGGCTGATCG
AAGAAGGCACCAGTTTCAAA

Product: acetoin:2,6-dichlorophenolindophenol oxidoreductase alpha subunit

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-beta; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit beta [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MPNKSYRNAIKEAIELEMRRDPTVIVVGEDVRGGHGGKNTDENKLEGFGGVLGVTKGLWTEFGSERVIDTPITESAIIGM
TAGAAATGLRPVADLMFMDFYGVCHDMLYNQAAKFRYMFGGKAKAPMVVRGMIGAGFSAAAQHSQSPYNVFASVPGLKVV
VPSSPYDVKGLLIQAIRDNDPVVFCEHKLLYDIKGEAPDEAYTIPFGVANYTREGTDVTIIALSLMVHRANEVADKLAKE
GISVEVVDPRTVSPLDEDGILESVASTGRVVIVDESAARCGFGHDVAALIAQKGFHYLKAPIELVTPPHTPIPFSPVLEK
EWIPSVERIEQAVRKILEA

Sequences:

>Translated_339_residues
MPNKSYRNAIKEAIELEMRRDPTVIVVGEDVRGGHGGKNTDENKLEGFGGVLGVTKGLWTEFGSERVIDTPITESAIIGM
TAGAAATGLRPVADLMFMDFYGVCHDMLYNQAAKFRYMFGGKAKAPMVVRGMIGAGFSAAAQHSQSPYNVFASVPGLKVV
VPSSPYDVKGLLIQAIRDNDPVVFCEHKLLYDIKGEAPDEAYTIPFGVANYTREGTDVTIIALSLMVHRANEVADKLAKE
GISVEVVDPRTVSPLDEDGILESVASTGRVVIVDESAARCGFGHDVAALIAQKGFHYLKAPIELVTPPHTPIPFSPVLEK
EWIPSVERIEQAVRKILEA
>Mature_338_residues
PNKSYRNAIKEAIELEMRRDPTVIVVGEDVRGGHGGKNTDENKLEGFGGVLGVTKGLWTEFGSERVIDTPITESAIIGMT
AGAAATGLRPVADLMFMDFYGVCHDMLYNQAAKFRYMFGGKAKAPMVVRGMIGAGFSAAAQHSQSPYNVFASVPGLKVVV
PSSPYDVKGLLIQAIRDNDPVVFCEHKLLYDIKGEAPDEAYTIPFGVANYTREGTDVTIIALSLMVHRANEVADKLAKEG
ISVEVVDPRTVSPLDEDGILESVASTGRVVIVDESAARCGFGHDVAALIAQKGFHYLKAPIELVTPPHTPIPFSPVLEKE
WIPSVERIEQAVRKILEA

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde, in vitro. The beta subunit is probably not the catalytic subunit of the enzyme [H]

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI156564403, Length=338, Percent_Identity=38.1656804733728, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI4557353, Length=334, Percent_Identity=37.125748502994, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI34101272, Length=334, Percent_Identity=37.125748502994, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI291084858, Length=338, Percent_Identity=36.094674556213, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI133778974, Length=174, Percent_Identity=30.4597701149425, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=338, Percent_Identity=37.2781065088757, Blast_Score=214, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17506935, Length=332, Percent_Identity=38.5542168674699, Blast_Score=203, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6319698, Length=337, Percent_Identity=38.5756676557863, Blast_Score=218, Evalue=1e-57,
Organism=Drosophila melanogaster, GI21358145, Length=338, Percent_Identity=36.9822485207101, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24650940, Length=338, Percent_Identity=36.9822485207101, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI160714832, Length=335, Percent_Identity=37.0149253731343, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI160714828, Length=335, Percent_Identity=37.0149253731343, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24650943, Length=96, Percent_Identity=37.5, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24650945, Length=96, Percent_Identity=37.5, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: 1.2.4.1

Molecular weight: Translated: 36654; Mature: 36523

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNKSYRNAIKEAIELEMRRDPTVIVVGEDVRGGHGGKNTDENKLEGFGGVLGVTKGLWT
CCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHH
EFGSERVIDTPITESAIIGMTAGAAATGLRPVADLMFMDFYGVCHDMLYNQAAKFRYMFG
HCCCCCEECCCCCCCCEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
GKAKAPMVVRGMIGAGFSAAAQHSQSPYNVFASVPGLKVVVPSSPYDVKGLLIQAIRDND
CCCCCCEEEEEHHCCCHHHHHHCCCCCCEEEECCCCEEEEECCCCCCHHHHHHEEECCCC
PVVFCEHKLLYDIKGEAPDEAYTIPFGVANYTREGTDVTIIALSLMVHRANEVADKLAKE
CEEEECCEEEEEECCCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHC
GISVEVVDPRTVSPLDEDGILESVASTGRVVIVDESAARCGFGHDVAALIAQKGFHYLKA
CCEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCHHHHC
PIELVTPPHTPIPFSPVLEKEWIPSVERIEQAVRKILEA
CCEEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PNKSYRNAIKEAIELEMRRDPTVIVVGEDVRGGHGGKNTDENKLEGFGGVLGVTKGLWT
CCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHH
EFGSERVIDTPITESAIIGMTAGAAATGLRPVADLMFMDFYGVCHDMLYNQAAKFRYMFG
HCCCCCEECCCCCCCCEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
GKAKAPMVVRGMIGAGFSAAAQHSQSPYNVFASVPGLKVVVPSSPYDVKGLLIQAIRDND
CCCCCCEEEEEHHCCCHHHHHHCCCCCCEEEECCCCEEEEECCCCCCHHHHHHEEECCCC
PVVFCEHKLLYDIKGEAPDEAYTIPFGVANYTREGTDVTIIALSLMVHRANEVADKLAKE
CEEEECCEEEEEECCCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHC
GISVEVVDPRTVSPLDEDGILESVASTGRVVIVDESAARCGFGHDVAALIAQKGFHYLKA
CCEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCHHHHC
PIELVTPPHTPIPFSPVLEKEWIPSVERIEQAVRKILEA
CCEEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2061286 [H]