Click here to switch to the map view.

The map label for this gene is dut

Identifier: 50084110

GI number: 50084110

Start: 884818

End: 885270

Strand: Direct

Name: dut

Synonym: ACIAD0901

Alternate gene names: 50084110

Gene position: 884818-885270 (Clockwise)

Preceding gene: 50084109

Following gene: 50084111

Centisome position: 24.59

GC content: 42.83

Gene sequence:

>453_bases
ATGAAAGTCCAAGTTAAAATTTTAGATCAGCGTTTAGGTCAGGAATGGCCTTTGCCTTCTTATGCCACTACGGGTTCTGC
GGGTCTAGACCTTCGTGCTTGTCTGGATGAGGCCATTCAGATTGAACCAGGTCAAACTGTTTTAATCAAAACAGGCATGG
CGATTTATATTCATGATACTAACTTTGCTGGTTTGATTTTGCCGCGTTCAGGTTTAGGTCATAAGCATGGTATTGTTTTA
GGCAATCTGGTGGGATTAATTGATTCAGATTATCAGGGTGAACTGATGATTTCAGTTTGGAATCGTGGTCAAAACACCTT
TACTCTGGAACCAGGTGAGCGTTTAGCGCAGTATGTTCTGGTTCCAGTAATTCAGGCTGAATTTGAGCAGGTTGAAGAAT
TTGTAGCAACCGATCGTGGTGCGGGTGGCTTTGGTCACACAGGTCAGAAATAA

Upstream 100 bases:

>100_bases
GCTGGCAGGCAATGTTGATCCGAAAGAGCAGGCTCAGCAGTATTTAGCCAATTTAAAAGCGTTATTAAAGAAATAATTGA
TAAACCAATTAGGAACTGTA

Downstream 100 bases:

>100_bases
GCCAAATTGTTGAAAATAAATGTCTGTGTTAACAGACATTTATTTATTAGTAATTGAATGTTACAAAAAATTATATTAAA
AATGTTTGATTGTGTTTTGC

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MKVQVKILDQRLGQEWPLPSYATTGSAGLDLRACLDEAIQIEPGQTVLIKTGMAIYIHDTNFAGLILPRSGLGHKHGIVL
GNLVGLIDSDYQGELMISVWNRGQNTFTLEPGERLAQYVLVPVIQAEFEQVEEFVATDRGAGGFGHTGQK

Sequences:

>Translated_150_residues
MKVQVKILDQRLGQEWPLPSYATTGSAGLDLRACLDEAIQIEPGQTVLIKTGMAIYIHDTNFAGLILPRSGLGHKHGIVL
GNLVGLIDSDYQGELMISVWNRGQNTFTLEPGERLAQYVLVPVIQAEFEQVEEFVATDRGAGGFGHTGQK
>Mature_150_residues
MKVQVKILDQRLGQEWPLPSYATTGSAGLDLRACLDEAIQIEPGQTVLIKTGMAIYIHDTNFAGLILPRSGLGHKHGIVL
GNLVGLIDSDYQGELMISVWNRGQNTFTLEPGERLAQYVLVPVIQAEFEQVEEFVATDRGAGGFGHTGQK

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI4503423, Length=139, Percent_Identity=34.5323741007194, Blast_Score=86, Evalue=9e-18,
Organism=Homo sapiens, GI70906444, Length=139, Percent_Identity=34.5323741007194, Blast_Score=86, Evalue=9e-18,
Organism=Homo sapiens, GI70906441, Length=139, Percent_Identity=34.5323741007194, Blast_Score=85, Evalue=3e-17,
Organism=Escherichia coli, GI1790071, Length=149, Percent_Identity=69.7986577181208, Blast_Score=234, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI71988561, Length=134, Percent_Identity=38.8059701492537, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319729, Length=133, Percent_Identity=33.8345864661654, Blast_Score=72, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921126, Length=151, Percent_Identity=29.1390728476821, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24583610, Length=151, Percent_Identity=29.1390728476821, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_ACIAD (Q6FDR0)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045620.1
- ProteinModelPortal:   Q6FDR0
- SMR:   Q6FDR0
- STRING:   Q6FDR0
- GeneID:   2879771
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0901
- NMPDR:   fig|62977.3.peg.902
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- PhylomeDB:   Q6FDR0
- ProtClustDB:   PRK00601
- BioCyc:   ASP62977:ACIAD0901-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16347; Mature: 16347

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: BINDING 82-82 BINDING 96-96

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVQVKILDQRLGQEWPLPSYATTGSAGLDLRACLDEAIQIEPGQTVLIKTGMAIYIHDT
CEEEEEEHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCEEEEECCEEEEEECC
NFAGLILPRSGLGHKHGIVLGNLVGLIDSDYQGELMISVWNRGQNTFTLEPGERLAQYVL
CCEEEEEECCCCCCCCCEEEHHHHHHCCCCCCCEEEEEEECCCCEEEEECCHHHHHHHHH
VPVIQAEFEQVEEFVATDRGAGGFGHTGQK
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVQVKILDQRLGQEWPLPSYATTGSAGLDLRACLDEAIQIEPGQTVLIKTGMAIYIHDT
CEEEEEEHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCEEEEECCEEEEEECC
NFAGLILPRSGLGHKHGIVLGNLVGLIDSDYQGELMISVWNRGQNTFTLEPGERLAQYVL
CCEEEEEECCCCCCCCCEEEHHHHHHCCCCCCCEEEEEEECCCCEEEEECCHHHHHHHHH
VPVIQAEFEQVEEFVATDRGAGGFGHTGQK
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA