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The map label for this gene is rluB [H]

Identifier: 50084073

GI number: 50084073

Start: 848321

End: 849262

Strand: Reverse

Name: rluB [H]

Synonym: ACIAD0861

Alternate gene names: 50084073

Gene position: 849262-848321 (Counterclockwise)

Preceding gene: 50084074

Following gene: 50084072

Centisome position: 23.6

GC content: 44.37

Gene sequence:

>942_bases
ATGAGTGAAAAATTGCAAAAAGTATTAGCGCGAATTGGTTTAGGTTCTCGCAGATATATGGAAGAGGTCATTGCCGCGGG
TCGCGTCAGTGTGAATGGCCAAATTGCTCAAGTTGGTGAAAGGATTGAACCAGGCGACGAATTAAGGATTGATGGTCGCA
AGGTACAATTTCAGATTGAAGACGAAATTCGTCGTCGTGTATTGATTTATTACAAACCAGAAGGTGAAATCTGTTCTCGT
AACGATCCAGAACAGCGCCCAACCGTGTTTGATCATTTACCACAAATTGCCAATGACCGTTGGGTGATGGTAGGCCGTCT
AGATATCAACAGTACTGGGCTTTTGCTTTTCACAAATGATGGTGAACTTGCCAATCGTTTGATGCATCCATCCAATGAAA
TCGAACGCGAATATGCAGTACGCGTCATGGGTGAAGTGACGCCTCAAATTCGTCAAACCTTACTTAATGGTGTTGTTCTA
GACGATGGTCCAGCGAAATTTGAGTCTTTTTCTGAGCTTGGTGGTGACGGAATTAACCGTTGGTATCAGGTTGTGGTGAA
AGAAGGACGTAACCGTGAAGTCCGTCGTTTATTTGAATCACAAGGTCTTAAAGTCAGCCGTCTATTACGTACCCGTTATG
GTTCAGTGATTTTACCACGTGAACTCCGTACAGGCCGCTGGATAGAGTTAGATAAAGGCGATATTGATAATCTCACCAAA
CTGGTTGAGTTGAAACCACGTCAGGGCACTGGTCTTTATGGTATGGCAAAACGTCGTACAGAGCGTATGGTCGAAAAGCC
AATGGCTGCACGCCGTGGTGGATACTTGAGACAGCAGCGCCGTGACAATGAAAATGGCACTGAAAATAATAACGAAACCA
AACAACCTTATGCTGCTGCGCGAAAGACTGTGGCGGTAAATAAAGGATTTAAGAAGTTCTGA

Upstream 100 bases:

>100_bases
CCCTGCAGGATGCCAAGGACGCGTTTATGAGTTTGGATGTGAATACACCCAAGTCATAAATCGGTGAACTGTTGACAATT
ATTTCTAAGGTTATGCTGTC

Downstream 100 bases:

>100_bases
TCAATTCATTATGTAACCTAAAAAGAGTTCACTTTTGTGAACTCTTTTTTATTAAATTGTTTTTTTGAGAAATAGTTTAC
ATATATACAAGTCTGTATAT

Product: ribosomal large subunit pseudouridine synthase B (pseudouridylate synthase) (Uracil hydrolyase)

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSEKLQKVLARIGLGSRRYMEEVIAAGRVSVNGQIAQVGERIEPGDELRIDGRKVQFQIEDEIRRRVLIYYKPEGEICSR
NDPEQRPTVFDHLPQIANDRWVMVGRLDINSTGLLLFTNDGELANRLMHPSNEIEREYAVRVMGEVTPQIRQTLLNGVVL
DDGPAKFESFSELGGDGINRWYQVVVKEGRNREVRRLFESQGLKVSRLLRTRYGSVILPRELRTGRWIELDKGDIDNLTK
LVELKPRQGTGLYGMAKRRTERMVEKPMAARRGGYLRQQRRDNENGTENNNETKQPYAAARKTVAVNKGFKKF

Sequences:

>Translated_313_residues
MSEKLQKVLARIGLGSRRYMEEVIAAGRVSVNGQIAQVGERIEPGDELRIDGRKVQFQIEDEIRRRVLIYYKPEGEICSR
NDPEQRPTVFDHLPQIANDRWVMVGRLDINSTGLLLFTNDGELANRLMHPSNEIEREYAVRVMGEVTPQIRQTLLNGVVL
DDGPAKFESFSELGGDGINRWYQVVVKEGRNREVRRLFESQGLKVSRLLRTRYGSVILPRELRTGRWIELDKGDIDNLTK
LVELKPRQGTGLYGMAKRRTERMVEKPMAARRGGYLRQQRRDNENGTENNNETKQPYAAARKTVAVNKGFKKF
>Mature_312_residues
SEKLQKVLARIGLGSRRYMEEVIAAGRVSVNGQIAQVGERIEPGDELRIDGRKVQFQIEDEIRRRVLIYYKPEGEICSRN
DPEQRPTVFDHLPQIANDRWVMVGRLDINSTGLLLFTNDGELANRLMHPSNEIEREYAVRVMGEVTPQIRQTLLNGVVLD
DGPAKFESFSELGGDGINRWYQVVVKEGRNREVRRLFESQGLKVSRLLRTRYGSVILPRELRTGRWIELDKGDIDNLTKL
VELKPRQGTGLYGMAKRRTERMVEKPMAARRGGYLRQQRRDNENGTENNNETKQPYAAARKTVAVNKGFKKF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=293, Percent_Identity=54.2662116040956, Blast_Score=299, Evalue=1e-82,
Organism=Escherichia coli, GI1790453, Length=244, Percent_Identity=30.327868852459, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788510, Length=241, Percent_Identity=28.2157676348548, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87081838, Length=181, Percent_Identity=31.4917127071823, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 35982; Mature: 35851

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS50889 S4 ; PS00061 ADH_SHORT ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKLQKVLARIGLGSRRYMEEVIAAGRVSVNGQIAQVGERIEPGDELRIDGRKVQFQIE
CCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEECCEEEEEEEH
DEIRRRVLIYYKPEGEICSRNDPEQRPTVFDHLPQIANDRWVMVGRLDINSTGLLLFTND
HHHCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCEEEEEECC
GELANRLMHPSNEIEREYAVRVMGEVTPQIRQTLLNGVVLDDGPAKFESFSELGGDGINR
HHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCEEECCCCHHHHHHHHHCCHHHHH
WYQVVVKEGRNREVRRLFESQGLKVSRLLRTRYGSVILPRELRTGRWIELDKGDIDNLTK
HHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCEEECHHCCCCCEEEECCCCHHHHHH
LVELKPRQGTGLYGMAKRRTERMVEKPMAARRGGYLRQQRRDNENGTENNNETKQPYAAA
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHH
RKTVAVNKGFKKF
HHHHHCCCCCCCC
>Mature Secondary Structure 
SEKLQKVLARIGLGSRRYMEEVIAAGRVSVNGQIAQVGERIEPGDELRIDGRKVQFQIE
CHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEECCEEEEEEEH
DEIRRRVLIYYKPEGEICSRNDPEQRPTVFDHLPQIANDRWVMVGRLDINSTGLLLFTND
HHHCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCEEEEEECC
GELANRLMHPSNEIEREYAVRVMGEVTPQIRQTLLNGVVLDDGPAKFESFSELGGDGINR
HHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCEEECCCCHHHHHHHHHCCHHHHH
WYQVVVKEGRNREVRRLFESQGLKVSRLLRTRYGSVILPRELRTGRWIELDKGDIDNLTK
HHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCEEECHHCCCCCEEEECCCCHHHHHH
LVELKPRQGTGLYGMAKRRTERMVEKPMAARRGGYLRQQRRDNENGTENNNETKQPYAAA
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHH
RKTVAVNKGFKKF
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]