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The map label for this gene is nuoM [H]

Identifier: 50083960

GI number: 50083960

Start: 726225

End: 727823

Strand: Direct

Name: nuoM [H]

Synonym: ACIAD0742

Alternate gene names: 50083960

Gene position: 726225-727823 (Clockwise)

Preceding gene: 50083959

Following gene: 50083961

Centisome position: 20.18

GC content: 41.65

Gene sequence:

>1599_bases
ATGGAAAACAATTTAATTTTACCCGCGCTGATACTCGTTCCCTTCATTGCAGGTTTTCTTTGCTGGATCATTGATAAACT
TGACCAGCAACTTCCACGCTGGATTGCCCTGATTGGTATGCTAATTACCTTGGGCTTAACTTTGGCACTTTGGCAGCATG
GTACTTATCAGTACGAGCTAGGGGCAAAAGTTCCAACGTGGTCTTCAGAATTTTATATTCCTTGGATTAAAACACTTGGA
ATTAATATCCATCTGGCTGTAGATGGTCTTTCTCTTATGATGGTTGGATTAACTGCATTACTTGGTGTATTGGCAGTCGG
CTGTTCATGGGGCGAAATTCAAAAAAATGTTGGTTTTTTCCACCTTAACCTGTTGTGGTCATTGGGTGGGGTGATTGGTG
TATTCCTTGCAATTGATATGTTCCTCTTCTTCTTCTTCTGGGAGATGATGCTGGTTCCAATCTATTTCTTGATTGCACTG
TGGGGACACAAAGGATCGAATGGCCGTTCGCGTGTATATGCAGCGACTAAATTTTTCCTTTATACCCAGATTGCTGGTTT
GATCATGTTGATCGGTATTCTTGGTCTTGTAGTTTATGGCTACATGATGACAGGTATGATCGGCTTCGATTATAACTACT
TGCTTGCAGTTGCAAACAACTTGCCAGCAGGTGTTGCTTATGCCTTTATGCTGTGCTTCTTTATTGGTTTTGCCGTAAAA
CTTCCTGTGTTTCCTTTACACGGCTGGTTACCAGATGCACATGCACAAGCACCAACAGCAGGTTCTGTGGACTTGGCAGG
GATCTTGATCAAAACGGCTGCCTATGGTCTGCTTCGTTTTGTGATTCCATTCTTTCCAGCGGCGTCTGCACAATTTGCAG
ATATCGCAATCATTTTTGGTTTGATTGGTATTTTCTACGGTGCTTGGTGTGCTTACCAGCAAACAGACATGAAACGTTTG
CTTGCTTATACCTCTATTTCACACATGGGCTTTGTACTACTTGCAATTTATGCAGGTAATATTTTGACGTATCAAGGTCT
GATGATCATGATGCTGGCACACGGTTTGTCTTCTGCTGCGCTATTTATTATGTGCGGTCAGATTTACGAGCGTTTGCATA
CACGTGATCTACGTTTGATGGGCGGTATTCGTGGTCAGTTCCGCTACTTGCCATTTTTCTTAATGTTCTTTGTTGCAGCA
CTTGTGGGTATTCCTGGTTTAGGTAACTTTATTGGTGAATTCCTGATTTTGATGGGATCTTTCAACAAGTATCCAACATT
CACAATTATTGCGGCTGTAAGTTTGGTATTTGCAGGATTGTACGGTTTGATCTTGATCCATCGTGCATTATTTGGCGAAC
CAAATCCAGAGCAGGCTCAGCATTACAAACAACCACTGAAAGATCTTTCTACTCGTGAAGTAGGAATTTTGATGATCTGT
TTAATTGGTCTGGTTTGGTTAGGAATCTATCCTCAAACTTTCCTCGATATTTCTCATTCATCAATGCAATGGTTAACCAA
TGGTTATGCTCCTGTCAGTCAAATTGTTGATGTTACTCAACAAGCAGTAGCTCAGCTTAGCGATGTGGAGATCCAATAA

Upstream 100 bases:

>100_bases
CGTCAAACAGGTTCATTGCGTGAATACGCGTCAAGTATGTCACTCGGTGTAGTGGTGTTATTGATGATCCTGATCATTAT
TCAGGTAGTGGGGAAATAAA

Downstream 100 bases:

>100_bases
GCCATGAACTTCACAATGTCTTTTTCTCAGCTTATGCCGTTAGCACCTGTCATGATTGTGGCTTTAACCGCAGTCGTGGT
AATGATCCTGATTTCGATTA

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M; NUO13 [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MENNLILPALILVPFIAGFLCWIIDKLDQQLPRWIALIGMLITLGLTLALWQHGTYQYELGAKVPTWSSEFYIPWIKTLG
INIHLAVDGLSLMMVGLTALLGVLAVGCSWGEIQKNVGFFHLNLLWSLGGVIGVFLAIDMFLFFFFWEMMLVPIYFLIAL
WGHKGSNGRSRVYAATKFFLYTQIAGLIMLIGILGLVVYGYMMTGMIGFDYNYLLAVANNLPAGVAYAFMLCFFIGFAVK
LPVFPLHGWLPDAHAQAPTAGSVDLAGILIKTAAYGLLRFVIPFFPAASAQFADIAIIFGLIGIFYGAWCAYQQTDMKRL
LAYTSISHMGFVLLAIYAGNILTYQGLMIMMLAHGLSSAALFIMCGQIYERLHTRDLRLMGGIRGQFRYLPFFLMFFVAA
LVGIPGLGNFIGEFLILMGSFNKYPTFTIIAAVSLVFAGLYGLILIHRALFGEPNPEQAQHYKQPLKDLSTREVGILMIC
LIGLVWLGIYPQTFLDISHSSMQWLTNGYAPVSQIVDVTQQAVAQLSDVEIQ

Sequences:

>Translated_532_residues
MENNLILPALILVPFIAGFLCWIIDKLDQQLPRWIALIGMLITLGLTLALWQHGTYQYELGAKVPTWSSEFYIPWIKTLG
INIHLAVDGLSLMMVGLTALLGVLAVGCSWGEIQKNVGFFHLNLLWSLGGVIGVFLAIDMFLFFFFWEMMLVPIYFLIAL
WGHKGSNGRSRVYAATKFFLYTQIAGLIMLIGILGLVVYGYMMTGMIGFDYNYLLAVANNLPAGVAYAFMLCFFIGFAVK
LPVFPLHGWLPDAHAQAPTAGSVDLAGILIKTAAYGLLRFVIPFFPAASAQFADIAIIFGLIGIFYGAWCAYQQTDMKRL
LAYTSISHMGFVLLAIYAGNILTYQGLMIMMLAHGLSSAALFIMCGQIYERLHTRDLRLMGGIRGQFRYLPFFLMFFVAA
LVGIPGLGNFIGEFLILMGSFNKYPTFTIIAAVSLVFAGLYGLILIHRALFGEPNPEQAQHYKQPLKDLSTREVGILMIC
LIGLVWLGIYPQTFLDISHSSMQWLTNGYAPVSQIVDVTQQAVAQLSDVEIQ
>Mature_532_residues
MENNLILPALILVPFIAGFLCWIIDKLDQQLPRWIALIGMLITLGLTLALWQHGTYQYELGAKVPTWSSEFYIPWIKTLG
INIHLAVDGLSLMMVGLTALLGVLAVGCSWGEIQKNVGFFHLNLLWSLGGVIGVFLAIDMFLFFFFWEMMLVPIYFLIAL
WGHKGSNGRSRVYAATKFFLYTQIAGLIMLIGILGLVVYGYMMTGMIGFDYNYLLAVANNLPAGVAYAFMLCFFIGFAVK
LPVFPLHGWLPDAHAQAPTAGSVDLAGILIKTAAYGLLRFVIPFFPAASAQFADIAIIFGLIGIFYGAWCAYQQTDMKRL
LAYTSISHMGFVLLAIYAGNILTYQGLMIMMLAHGLSSAALFIMCGQIYERLHTRDLRLMGGIRGQFRYLPFFLMFFVAA
LVGIPGLGNFIGEFLILMGSFNKYPTFTIIAAVSLVFAGLYGLILIHRALFGEPNPEQAQHYKQPLKDLSTREVGILMIC
LIGLVWLGIYPQTFLDISHSSMQWLTNGYAPVSQIVDVTQQAVAQLSDVEIQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=364, Percent_Identity=29.6703296703297, Blast_Score=161, Evalue=1e-39,
Organism=Escherichia coli, GI1788613, Length=507, Percent_Identity=61.1439842209073, Blast_Score=611, Evalue=1e-176,
Organism=Escherichia coli, GI1788831, Length=265, Percent_Identity=30.188679245283, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788827, Length=353, Percent_Identity=26.0623229461756, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI145693160, Length=435, Percent_Identity=25.9770114942529, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788614, Length=458, Percent_Identity=24.8908296943231, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI2367154, Length=271, Percent_Identity=25.0922509225092, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 59021; Mature: 59021

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENNLILPALILVPFIAGFLCWIIDKLDQQLPRWIALIGMLITLGLTLALWQHGTYQYEL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
GAKVPTWSSEFYIPWIKTLGINIHLAVDGLSLMMVGLTALLGVLAVGCSWGEIQKNVGFF
CCCCCCCCCCEEECHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHH
HLNLLWSLGGVIGVFLAIDMFLFFFFWEMMLVPIYFLIALWGHKGSNGRSRVYAATKFFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
YTQIAGLIMLIGILGLVVYGYMMTGMIGFDYNYLLAVANNLPAGVAYAFMLCFFIGFAVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LPVFPLHGWLPDAHAQAPTAGSVDLAGILIKTAAYGLLRFVIPFFPAASAQFADIAIIFG
CCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LIGIFYGAWCAYQQTDMKRLLAYTSISHMGFVLLAIYAGNILTYQGLMIMMLAHGLSSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHH
LFIMCGQIYERLHTRDLRLMGGIRGQFRYLPFFLMFFVAALVGIPGLGNFIGEFLILMGS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
FNKYPTFTIIAAVSLVFAGLYGLILIHRALFGEPNPEQAQHYKQPLKDLSTREVGILMIC
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
LIGLVWLGIYPQTFLDISHSSMQWLTNGYAPVSQIVDVTQQAVAQLSDVEIQ
HHHHHHHHCCHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHCCHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]