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The map label for this gene is nuoL [H]

Identifier: 50083959

GI number: 50083959

Start: 724328

End: 726223

Strand: Direct

Name: nuoL [H]

Synonym: ACIAD0741

Alternate gene names: 50083959

Gene position: 724328-726223 (Clockwise)

Preceding gene: 50083958

Following gene: 50083960

Centisome position: 20.13

GC content: 44.94

Gene sequence:

>1896_bases
ATGAGTACTTTATATTTAACGATTCTTTTTCCGCTGATCGGTTTTATTTTATTGGCGGCAGGGCGCGATAAACTTTCTGA
AAATGTAGCCGCGATTGTGGGTGTCGGTTCAGTTGGTTTATCCGCATTATTTGCGCTTCTTGCGGGATTGGCATTTACCA
GCAGTGGTCAACCTGTTTTTGTTCAGCATTTATGGACATGGTTTAATGTCGGCGGCTTTGCACCTGGCATTACCTTGCAT
TTAGATGGTTTATCATTGCTCATGATGGGCATGATTACTGGCGTTGGTTTTCTGATCCACATCTTTGCATCATGGTATAT
GCGTGGTGAAGAGGGCTATGCACGTTTCTTCTCCTACTTTAACCTGTTTGTAGCCAGCATGCTGGTGTTGGTATTGGGTG
ATAACCTTGCGTTATTGTTCTTGGGTTGGGAAGGTGTAGGTCTGTGTTCATACCTATTGATTGGCTTTTACTACTCAAAT
CCTGCCAATGGCTGGGCTGCCATCAAAGCATTTACAGTGACCCGTGTGGGTGATGTATTCTTGCTGATTGCACTCTTCTT
GCTTTACCAACAGTTTGGTACGCTCAACACGCAGTACATTGTGGAAAATGCAGCGTCAGTGATGACTCAAAGTTCATCAC
TTTCGATCTGGACCGCATTAATGTTGTTCTTGGGTGCAGCAGGTAAATCGGCACAAATTCCATTACAAACATGGCTTGCT
GATGCAATGGCAGGCCCAACACCAGTTTCGGCATTGATTCACGCGGCAACGATGGTTACTGCGGGTGTTTACTTAACCAG
TCGTATGCATACAGTGATCGAAATGGCGCCTGCTGTGATGCAGTTTATTTCAATCACGGGTGCAGTGACACTCCTTGTTG
CAGGTTTTGCGGCACTGGTTCAAACCGATATCAAACGTATCTTGGCTTACTCGACTATGAGTCAGCTCGGCTATATGTTT
ATGGCTGTGGGTGCAGAAGCTTATCAGGCTGGTCTGTTCCATATGTTGGCACATGCATTCTTCAAAGCATTACTGTTCTT
ATCATCTGGTGCAGTGATTCTGGCATGTCATCACGAACAGAACATTTTCAAAATGGGTGGTCTGCGTAAAAAGATCCCAT
TTGTATTCTGGTGTTTTGTGATTGGTGGTGGTGCATTGGCTGCGCTTCCGATCGTAACGATTGGCTTCTTCTCAAAAGAT
GCAATTTTGGGTGCTGTATACGCGCAGTCTACGCTTGCGAACCTTCCGCTTTACAATACCTTGTACTGGGTCGGTGTTGC
TGGTGCATTCCTCACCTCGATTTATACCTTCCGTTTAATCTGGGTAGTCTTCTTTGGCGAAGAAAAAACCGAAGCACATG
CCATTCATGGCGTGACTTACTGGGGACCACTCGCAATTCTGGCATTTCTATCGACAGGTGTGGGTTATTTCCTTCAAGCA
CCCGTCTTAAAGCTACTGTCAGGGGCAAACATTCCAGCATTCACTATTCCTGAGCAATTAGAAGCAGCAGTGTCTCATGC
TGAACATCTGGCAAGTGGTGTTGCGATTGTTGGATTGGTTATTGGAATTGCATTGTTTGTATTTGCATATAATGCGGTTA
AATCTTTTGCTGCAACAGGACTCGGTTCTGGTCTGGCACACATTTGTCGTACGGCTTTTGGTTTCGATGCACTCTATGAC
ATCGTGTTTGTTAAACCCTATTTGCTCATCGCGAAAATTTTAGGTCGTGATCCGATTGATGGTTTGTGGTTAGTGCTTCC
AGCTTTGGTTAAAGGTGGTAATAGCTTTACCAGTGCCCGTCAAACAGGTTCATTGCGTGAATACGCGTCAAGTATGTCAC
TCGGTGTAGTGGTGTTATTGATGATCCTGATCATTATTCAGGTAGTGGGGAAATAA

Upstream 100 bases:

>100_bases
CCCTTGCTGCGGCAGAGGCATGTATCGGTCTTGCGATCGTCCTTCAGTTTTACCATCGCTTCCACCATCTGGATGTGGAT
GCTGCGAGTGAGATGCGTGG

Downstream 100 bases:

>100_bases
AATGGAAAACAATTTAATTTTACCCGCGCTGATACTCGTTCCCTTCATTGCAGGTTTTCTTTGCTGGATCATTGATAAAC
TTGACCAGCAACTTCCACGC

Product: NADH dehydrogenase subunit L

Products: NA

Alternate protein names: NADH dehydrogenase I subunit L; NDH-1 subunit L; NUO12 [H]

Number of amino acids: Translated: 631; Mature: 630

Protein sequence:

>631_residues
MSTLYLTILFPLIGFILLAAGRDKLSENVAAIVGVGSVGLSALFALLAGLAFTSSGQPVFVQHLWTWFNVGGFAPGITLH
LDGLSLLMMGMITGVGFLIHIFASWYMRGEEGYARFFSYFNLFVASMLVLVLGDNLALLFLGWEGVGLCSYLLIGFYYSN
PANGWAAIKAFTVTRVGDVFLLIALFLLYQQFGTLNTQYIVENAASVMTQSSSLSIWTALMLFLGAAGKSAQIPLQTWLA
DAMAGPTPVSALIHAATMVTAGVYLTSRMHTVIEMAPAVMQFISITGAVTLLVAGFAALVQTDIKRILAYSTMSQLGYMF
MAVGAEAYQAGLFHMLAHAFFKALLFLSSGAVILACHHEQNIFKMGGLRKKIPFVFWCFVIGGGALAALPIVTIGFFSKD
AILGAVYAQSTLANLPLYNTLYWVGVAGAFLTSIYTFRLIWVVFFGEEKTEAHAIHGVTYWGPLAILAFLSTGVGYFLQA
PVLKLLSGANIPAFTIPEQLEAAVSHAEHLASGVAIVGLVIGIALFVFAYNAVKSFAATGLGSGLAHICRTAFGFDALYD
IVFVKPYLLIAKILGRDPIDGLWLVLPALVKGGNSFTSARQTGSLREYASSMSLGVVVLLMILIIIQVVGK

Sequences:

>Translated_631_residues
MSTLYLTILFPLIGFILLAAGRDKLSENVAAIVGVGSVGLSALFALLAGLAFTSSGQPVFVQHLWTWFNVGGFAPGITLH
LDGLSLLMMGMITGVGFLIHIFASWYMRGEEGYARFFSYFNLFVASMLVLVLGDNLALLFLGWEGVGLCSYLLIGFYYSN
PANGWAAIKAFTVTRVGDVFLLIALFLLYQQFGTLNTQYIVENAASVMTQSSSLSIWTALMLFLGAAGKSAQIPLQTWLA
DAMAGPTPVSALIHAATMVTAGVYLTSRMHTVIEMAPAVMQFISITGAVTLLVAGFAALVQTDIKRILAYSTMSQLGYMF
MAVGAEAYQAGLFHMLAHAFFKALLFLSSGAVILACHHEQNIFKMGGLRKKIPFVFWCFVIGGGALAALPIVTIGFFSKD
AILGAVYAQSTLANLPLYNTLYWVGVAGAFLTSIYTFRLIWVVFFGEEKTEAHAIHGVTYWGPLAILAFLSTGVGYFLQA
PVLKLLSGANIPAFTIPEQLEAAVSHAEHLASGVAIVGLVIGIALFVFAYNAVKSFAATGLGSGLAHICRTAFGFDALYD
IVFVKPYLLIAKILGRDPIDGLWLVLPALVKGGNSFTSARQTGSLREYASSMSLGVVVLLMILIIIQVVGK
>Mature_630_residues
STLYLTILFPLIGFILLAAGRDKLSENVAAIVGVGSVGLSALFALLAGLAFTSSGQPVFVQHLWTWFNVGGFAPGITLHL
DGLSLLMMGMITGVGFLIHIFASWYMRGEEGYARFFSYFNLFVASMLVLVLGDNLALLFLGWEGVGLCSYLLIGFYYSNP
ANGWAAIKAFTVTRVGDVFLLIALFLLYQQFGTLNTQYIVENAASVMTQSSSLSIWTALMLFLGAAGKSAQIPLQTWLAD
AMAGPTPVSALIHAATMVTAGVYLTSRMHTVIEMAPAVMQFISITGAVTLLVAGFAALVQTDIKRILAYSTMSQLGYMFM
AVGAEAYQAGLFHMLAHAFFKALLFLSSGAVILACHHEQNIFKMGGLRKKIPFVFWCFVIGGGALAALPIVTIGFFSKDA
ILGAVYAQSTLANLPLYNTLYWVGVAGAFLTSIYTFRLIWVVFFGEEKTEAHAIHGVTYWGPLAILAFLSTGVGYFLQAP
VLKLLSGANIPAFTIPEQLEAAVSHAEHLASGVAIVGLVIGIALFVFAYNAVKSFAATGLGSGLAHICRTAFGFDALYDI
VFVKPYLLIAKILGRDPIDGLWLVLPALVKGGNSFTSARQTGSLREYASSMSLGVVVLLMILIIIQVVGK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=450, Percent_Identity=35.3333333333333, Blast_Score=238, Evalue=1e-62,
Organism=Escherichia coli, GI1788614, Length=615, Percent_Identity=56.5853658536585, Blast_Score=653, Evalue=0.0,
Organism=Escherichia coli, GI1788829, Length=358, Percent_Identity=31.8435754189944, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1788831, Length=483, Percent_Identity=28.5714285714286, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788827, Length=358, Percent_Identity=31.0055865921788, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI2367154, Length=287, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788613, Length=323, Percent_Identity=28.4829721362229, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI145693160, Length=334, Percent_Identity=26.6467065868263, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 67606; Mature: 67475

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLYLTILFPLIGFILLAAGRDKLSENVAAIVGVGSVGLSALFALLAGLAFTSSGQPVF
CCHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHEECCHHHHHHHHHHHHHHHHCCCCCCHH
VQHLWTWFNVGGFAPGITLHLDGLSLLMMGMITGVGFLIHIFASWYMRGEEGYARFFSYF
HHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
NLFVASMLVLVLGDNLALLFLGWEGVGLCSYLLIGFYYSNPANGWAAIKAFTVTRVGDVF
HHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LLIALFLLYQQFGTLNTQYIVENAASVMTQSSSLSIWTALMLFLGAAGKSAQIPLQTWLA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
DAMAGPTPVSALIHAATMVTAGVYLTSRMHTVIEMAPAVMQFISITGAVTLLVAGFAALV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTDIKRILAYSTMSQLGYMFMAVGAEAYQAGLFHMLAHAFFKALLFLSSGAVILACHHEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
NIFKMGGLRKKIPFVFWCFVIGGGALAALPIVTIGFFSKDAILGAVYAQSTLANLPLYNT
CHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHH
LYWVGVAGAFLTSIYTFRLIWVVFFGEEKTEAHAIHGVTYWGPLAILAFLSTGVGYFLQA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHCHHHHHHH
PVLKLLSGANIPAFTIPEQLEAAVSHAEHLASGVAIVGLVIGIALFVFAYNAVKSFAATG
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LGSGLAHICRTAFGFDALYDIVFVKPYLLIAKILGRDPIDGLWLVLPALVKGGNSFTSAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHH
QTGSLREYASSMSLGVVVLLMILIIIQVVGK
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STLYLTILFPLIGFILLAAGRDKLSENVAAIVGVGSVGLSALFALLAGLAFTSSGQPVF
CHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHEECCHHHHHHHHHHHHHHHHCCCCCCHH
VQHLWTWFNVGGFAPGITLHLDGLSLLMMGMITGVGFLIHIFASWYMRGEEGYARFFSYF
HHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
NLFVASMLVLVLGDNLALLFLGWEGVGLCSYLLIGFYYSNPANGWAAIKAFTVTRVGDVF
HHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LLIALFLLYQQFGTLNTQYIVENAASVMTQSSSLSIWTALMLFLGAAGKSAQIPLQTWLA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
DAMAGPTPVSALIHAATMVTAGVYLTSRMHTVIEMAPAVMQFISITGAVTLLVAGFAALV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTDIKRILAYSTMSQLGYMFMAVGAEAYQAGLFHMLAHAFFKALLFLSSGAVILACHHEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
NIFKMGGLRKKIPFVFWCFVIGGGALAALPIVTIGFFSKDAILGAVYAQSTLANLPLYNT
CHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHH
LYWVGVAGAFLTSIYTFRLIWVVFFGEEKTEAHAIHGVTYWGPLAILAFLSTGVGYFLQA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHCHHHHHHH
PVLKLLSGANIPAFTIPEQLEAAVSHAEHLASGVAIVGLVIGIALFVFAYNAVKSFAATG
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LGSGLAHICRTAFGFDALYDIVFVKPYLLIAKILGRDPIDGLWLVLPALVKGGNSFTSAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHH
QTGSLREYASSMSLGVVVLLMILIIIQVVGK
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7690854; 9205837; 9278503 [H]