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The map label for this gene is nuoI

Identifier: 50083956

GI number: 50083956

Start: 722963

End: 723505

Strand: Direct

Name: nuoI

Synonym: ACIAD0738

Alternate gene names: 50083956

Gene position: 722963-723505 (Clockwise)

Preceding gene: 50083955

Following gene: 50083957

Centisome position: 20.09

GC content: 43.83

Gene sequence:

>543_bases
ATGTATAAAGTTCTAGCTGGAGTTGGGTCAATCGTTCGTACATTGTGGATGGTTTTCACTCACATTACACGTAAACGTGA
CACAATTCTGTATCCAGAAGTACCAGCAGAGGAAATTGTACCACCTCGCTATCGTGGTCGTATTGTTTTGACACGTGATC
CGGACGGGGAAGAGCGCTGTGTCGCGTGTAACCTCTGTGCGGTGGCATGTCCAGTGGGTTGTATTTCACTGCAAAAAGCA
GAGAAAGAAGACGGTCGCTGGTATCCTGAATTTTTCCGTATCAATTTTTCACGTTGTATCTTCTGTGGTATGTGTGAAGA
AGCATGTCCGACCACTGCCATCCAGATGACGCCGGATTTTGAGTTGGGCGAATACGTGCGTCAGGATTTGGTTTATGAAA
AAGAAAACTTACTGATTTCTGGTCCTGGTAAATATCCTGATTACAACTTTTATCGTGTTGCTGGTATGGCAATCGATGGT
AAAGAAAAAGGTCAGGCACAAAAAGAAAGTGCACCAATTGATGTACGGAGTTTATTACCATGA

Upstream 100 bases:

>100_bases
TTTTGGTTGGAAAGTTTGTTTGCCGCTGGCGTTGGTCAACCTGATGGTGACTGGTGCTGTGATTCTGATGAATCAGGCCT
AAGACAGCAGGGAGTACAAA

Downstream 100 bases:

>100_bases
TGTGGCCATTTTATTTGATGGCTCTTGTGGCCATTATCTCGACGATTCGTGTTGTGACTAACACGAATCCAGTACATGCT
TTATTAAGCTTGATTGTTTC

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MYKVLAGVGSIVRTLWMVFTHITRKRDTILYPEVPAEEIVPPRYRGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKA
EKEDGRWYPEFFRINFSRCIFCGMCEEACPTTAIQMTPDFELGEYVRQDLVYEKENLLISGPGKYPDYNFYRVAGMAIDG
KEKGQAQKESAPIDVRSLLP

Sequences:

>Translated_180_residues
MYKVLAGVGSIVRTLWMVFTHITRKRDTILYPEVPAEEIVPPRYRGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKA
EKEDGRWYPEFFRINFSRCIFCGMCEEACPTTAIQMTPDFELGEYVRQDLVYEKENLLISGPGKYPDYNFYRVAGMAIDG
KEKGQAQKESAPIDVRSLLP
>Mature_180_residues
MYKVLAGVGSIVRTLWMVFTHITRKRDTILYPEVPAEEIVPPRYRGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKA
EKEDGRWYPEFFRINFSRCIFCGMCEEACPTTAIQMTPDFELGEYVRQDLVYEKENLLISGPGKYPDYNFYRVAGMAIDG
KEKGQAQKESAPIDVRSLLP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=131, Percent_Identity=38.9312977099237, Blast_Score=104, Evalue=4e-23,
Organism=Escherichia coli, GI1788617, Length=180, Percent_Identity=77.2222222222222, Blast_Score=299, Evalue=9e-83,
Organism=Caenorhabditis elegans, GI17555194, Length=131, Percent_Identity=36.6412213740458, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17864306, Length=133, Percent_Identity=39.0977443609023, Blast_Score=109, Evalue=1e-24,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_ACIAD (Q6FE64)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045466.1
- HSSP:   Q56224
- ProteinModelPortal:   Q6FE64
- SMR:   Q6FE64
- STRING:   Q6FE64
- GeneID:   2879587
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0738
- NMPDR:   fig|62977.3.peg.545
- eggNOG:   COG1143
- HOGENOM:   HBG724359
- OMA:   RYWSVAG
- PhylomeDB:   Q6FE64
- ProtClustDB:   PRK05888
- BioCyc:   ASP62977:ACIAD0738-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 20442; Mature: 20442

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKVLAGVGSIVRTLWMVFTHITRKRDTILYPEVPAEEIVPPRYRGRIVLTRDPDGEERC
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHCCCCCCCCEEEEEECCCCCCCE
VACNLCAVACPVGCISLQKAEKEDGRWYPEFFRINFSRCIFCGMCEEACPTTAIQMTPDF
EEEHHHHHHHCHHHHHHHHCCCCCCCCCCHHEEECHHHEEEECCCHHCCCCCEEEECCCC
ELGEYVRQDLVYEKENLLISGPGKYPDYNFYRVAGMAIDGKEKGQAQKESAPIDVRSLLP
CHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MYKVLAGVGSIVRTLWMVFTHITRKRDTILYPEVPAEEIVPPRYRGRIVLTRDPDGEERC
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHCCCCCCCCEEEEEECCCCCCCE
VACNLCAVACPVGCISLQKAEKEDGRWYPEFFRINFSRCIFCGMCEEACPTTAIQMTPDF
EEEHHHHHHHCHHHHHHHHCCCCCCCCCCHHEEECHHHEEEECCCHHCCCCCEEEECCCC
ELGEYVRQDLVYEKENLLISGPGKYPDYNFYRVAGMAIDGKEKGQAQKESAPIDVRSLLP
CHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA