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The map label for this gene is rstB [H]

Identifier: 50083947

GI number: 50083947

Start: 711588

End: 713228

Strand: Direct

Name: rstB [H]

Synonym: ACIAD0727

Alternate gene names: 50083947

Gene position: 711588-713228 (Clockwise)

Preceding gene: 50083946

Following gene: 50083949

Centisome position: 19.77

GC content: 40.59

Gene sequence:

>1641_bases
GTGTTTAAACACAGTATTTTTTTGCGCATTTATGCAGGCTTGGTCGTTCTGGTCATGTTGGTTGCTTTATTTGGCTACTT
ACTTGTACAGATTATTAACTACCAGCGTGCTCAAGAATATCGTGAATCATTGACCGATGGCATTTCTTATGTCATTGGTG
AAGGAATTGCTCGGCAGCCTACAAAACAACAAAAATTAGACTGGATATCAGATGCCTCTGATCTACTTGAGTTGCCCATC
TATTATATCGATGCCAGCAAAATCGAGTTATCACGTACAGAGAAGAAACGTCTGGATGAGCAAAAATCAGTTGTTCGTTA
TGATGCTGAAACCTCAATTGCTTATGTGGTCATTGGCTTAAAGGATGATCCAAAACACTTCCTTTATATTAAGGTAGATA
AAATTGGTGAGCGTCAAATGAAGGCGCTGCCTATTTTTATCCTGGATTATCTGATTTTTTATCCTGGACAGGAAAAACAA
TACGTTCAAAAAATTCAGAAATATTTTTCTTATCCAATTAATATTTATGATGTTAAAGATTTGAATTTGGACTCGGAGCA
GATCGGGCGTTTACGTCAGGATCAAAGTGTGATGCTCTATAAAGATAATGCGACCATTCGTGGAACCACGATTTCGATTA
TCTCACCCATGCCAAACAATCCAATGCAAGCTTTGGTACTCGGGCCTGTGCCATTATTCAACTGGATGCCATTTCAACTC
TCTGCTGGAATTACCTTATTTAGTTTGTTTCTGCTTAGTCTGGGTGTTTATGGTTTAATTTTACCACTTGAACGAAAGAT
TCGTCAGGTACGTTATGCACTCAACCGCATGAAGTCTGGTGATTTATCATTACGTGTCCCGATTGAGGGAAGTGATGAAA
TGGCAAATCTGGCATCAAGTTATAATAATATGTCGGATCATATTCAGCGTTTGATTGAGGCACAACGTGAGTTGATGCGT
GCAGTTTCCCATGAACTAAGAACACCTGTTGCACGTATTCGTTTTGGAATGGAAATGCTCGCTGAAGAAGATGACTACAA
TTATCGTATTCAGCAAATGGAAATGATTGACCGTGATATTGAGGCACTGAATACCTTAATTGATGAAATCATGACCTATG
CCAAGTTGGAGCAGGGTACACCTTCGCTTGATTTTGAAGAGATCATACTGGTCGATCTGCTTAATCAGGTAGCCATTGAA
ACTGAAGCACTCAAAACCCAAAAAGAAATTGAACTCATTCCACCGCCCACTTATGTTATGGTCGATGCAGAGCGTCGTTA
TTTGCATCGTGTGGTTCAAAATCTGGTAGGTAATGCCGTGCGTTACTGCAATGATAAGGTGCGAATTTCTGGTGGAATTC
ATCCAGATGGTCATGCATTTATCTGTGTGGAAGACGATGGTGCGGGTATTGCTGAAGAAGATCGCGCGAGAGTATTTGAA
GCATTTGCTCGTTTAGATGATAGTCGTACACGTGCTTCTGGCGGCTATGGTTTGGGGCTTTCGATTGTGAGTCGTATTGC
CTATTGGTTTGGTGGAACCATCCAGGTAGATGAAAGTCCAGAATTAGGCGGCGCCCGCTTTGTGATGCAGTGGCCTGCAA
AACGTTTTAATGAAAATAGTAAAAAGAAAAAATCGATATAG

Upstream 100 bases:

>100_bases
AAAACTGTGCGCAGTAAAGGTTACTTGTTTGTGAAGGAAACCAATGGCTTGTAAAAGCAAGTAATGGTGATGAGTAAAAC
TTCCATTTAAGGCTGATCGA

Downstream 100 bases:

>100_bases
AACAAAAAAACAAGGCAGCGATAAACTGCCTTGTTTTTTAAATTTTAAAAGTTATTCGTACTGGTTCGCATCTGGTGCAA
CTAAAGATTTACCTTGCTGT

Product: sensory histidine kinase in two-component regulatory system with RstA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MFKHSIFLRIYAGLVVLVMLVALFGYLLVQIINYQRAQEYRESLTDGISYVIGEGIARQPTKQQKLDWISDASDLLELPI
YYIDASKIELSRTEKKRLDEQKSVVRYDAETSIAYVVIGLKDDPKHFLYIKVDKIGERQMKALPIFILDYLIFYPGQEKQ
YVQKIQKYFSYPINIYDVKDLNLDSEQIGRLRQDQSVMLYKDNATIRGTTISIISPMPNNPMQALVLGPVPLFNWMPFQL
SAGITLFSLFLLSLGVYGLILPLERKIRQVRYALNRMKSGDLSLRVPIEGSDEMANLASSYNNMSDHIQRLIEAQRELMR
AVSHELRTPVARIRFGMEMLAEEDDYNYRIQQMEMIDRDIEALNTLIDEIMTYAKLEQGTPSLDFEEIILVDLLNQVAIE
TEALKTQKEIELIPPPTYVMVDAERRYLHRVVQNLVGNAVRYCNDKVRISGGIHPDGHAFICVEDDGAGIAEEDRARVFE
AFARLDDSRTRASGGYGLGLSIVSRIAYWFGGTIQVDESPELGGARFVMQWPAKRFNENSKKKKSI

Sequences:

>Translated_546_residues
MFKHSIFLRIYAGLVVLVMLVALFGYLLVQIINYQRAQEYRESLTDGISYVIGEGIARQPTKQQKLDWISDASDLLELPI
YYIDASKIELSRTEKKRLDEQKSVVRYDAETSIAYVVIGLKDDPKHFLYIKVDKIGERQMKALPIFILDYLIFYPGQEKQ
YVQKIQKYFSYPINIYDVKDLNLDSEQIGRLRQDQSVMLYKDNATIRGTTISIISPMPNNPMQALVLGPVPLFNWMPFQL
SAGITLFSLFLLSLGVYGLILPLERKIRQVRYALNRMKSGDLSLRVPIEGSDEMANLASSYNNMSDHIQRLIEAQRELMR
AVSHELRTPVARIRFGMEMLAEEDDYNYRIQQMEMIDRDIEALNTLIDEIMTYAKLEQGTPSLDFEEIILVDLLNQVAIE
TEALKTQKEIELIPPPTYVMVDAERRYLHRVVQNLVGNAVRYCNDKVRISGGIHPDGHAFICVEDDGAGIAEEDRARVFE
AFARLDDSRTRASGGYGLGLSIVSRIAYWFGGTIQVDESPELGGARFVMQWPAKRFNENSKKKKSI
>Mature_546_residues
MFKHSIFLRIYAGLVVLVMLVALFGYLLVQIINYQRAQEYRESLTDGISYVIGEGIARQPTKQQKLDWISDASDLLELPI
YYIDASKIELSRTEKKRLDEQKSVVRYDAETSIAYVVIGLKDDPKHFLYIKVDKIGERQMKALPIFILDYLIFYPGQEKQ
YVQKIQKYFSYPINIYDVKDLNLDSEQIGRLRQDQSVMLYKDNATIRGTTISIISPMPNNPMQALVLGPVPLFNWMPFQL
SAGITLFSLFLLSLGVYGLILPLERKIRQVRYALNRMKSGDLSLRVPIEGSDEMANLASSYNNMSDHIQRLIEAQRELMR
AVSHELRTPVARIRFGMEMLAEEDDYNYRIQQMEMIDRDIEALNTLIDEIMTYAKLEQGTPSLDFEEIILVDLLNQVAIE
TEALKTQKEIELIPPPTYVMVDAERRYLHRVVQNLVGNAVRYCNDKVRISGGIHPDGHAFICVEDDGAGIAEEDRARVFE
AFARLDDSRTRASGGYGLGLSIVSRIAYWFGGTIQVDESPELGGARFVMQWPAKRFNENSKKKKSI

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=382, Percent_Identity=32.1989528795811, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1789808, Length=281, Percent_Identity=33.0960854092527, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1790346, Length=273, Percent_Identity=29.3040293040293, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1788393, Length=286, Percent_Identity=26.2237762237762, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1786600, Length=236, Percent_Identity=29.2372881355932, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1790551, Length=291, Percent_Identity=26.1168384879725, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1786783, Length=295, Percent_Identity=26.4406779661017, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081816, Length=270, Percent_Identity=27.037037037037, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789149, Length=246, Percent_Identity=28.4552845528455, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI145693157, Length=275, Percent_Identity=24, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082128, Length=298, Percent_Identity=27.1812080536913, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1786912, Length=261, Percent_Identity=28.3524904214559, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1788713, Length=230, Percent_Identity=24.7826086956522, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788549, Length=233, Percent_Identity=24.4635193133047, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI48994928, Length=243, Percent_Identity=26.7489711934156, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790861, Length=275, Percent_Identity=28.7272727272727, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787374, Length=306, Percent_Identity=25.4901960784314, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789403, Length=242, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1788279, Length=254, Percent_Identity=25.5905511811024, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 62561; Mature: 62561

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKHSIFLRIYAGLVVLVMLVALFGYLLVQIINYQRAQEYRESLTDGISYVIGEGIARQP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TKQQKLDWISDASDLLELPIYYIDASKIELSRTEKKRLDEQKSVVRYDAETSIAYVVIGL
CHHHHHHHHCCHHHHHHCCEEEEECCCEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
KDDPKHFLYIKVDKIGERQMKALPIFILDYLIFYPGQEKQYVQKIQKYFSYPINIYDVKD
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECC
LNLDSEQIGRLRQDQSVMLYKDNATIRGTTISIISPMPNNPMQALVLGPVPLFNWMPFQL
CCCCHHHHHHHHCCCEEEEEECCCEEEEEEEEEECCCCCCCCCEEEECCCCHHCCCCHHH
SAGITLFSLFLLSLGVYGLILPLERKIRQVRYALNRMKSGDLSLRVPIEGSDEMANLASS
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHH
YNNMSDHIQRLIEAQRELMRAVSHELRTPVARIRFGMEMLAEEDDYNYRIQQMEMIDRDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
EALNTLIDEIMTYAKLEQGTPSLDFEEIILVDLLNQVAIETEALKTQKEIELIPPPTYVM
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHCEEEECCCCEEE
VDAERRYLHRVVQNLVGNAVRYCNDKVRISGGIHPDGHAFICVEDDGAGIAEEDRARVFE
EECHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHH
AFARLDDSRTRASGGYGLGLSIVSRIAYWFGGTIQVDESPELGGARFVMQWPAKRFNENS
HHHHCCCCHHCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEECCHHHCCCCC
KKKKSI
HHHCCC
>Mature Secondary Structure
MFKHSIFLRIYAGLVVLVMLVALFGYLLVQIINYQRAQEYRESLTDGISYVIGEGIARQP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TKQQKLDWISDASDLLELPIYYIDASKIELSRTEKKRLDEQKSVVRYDAETSIAYVVIGL
CHHHHHHHHCCHHHHHHCCEEEEECCCEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
KDDPKHFLYIKVDKIGERQMKALPIFILDYLIFYPGQEKQYVQKIQKYFSYPINIYDVKD
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECC
LNLDSEQIGRLRQDQSVMLYKDNATIRGTTISIISPMPNNPMQALVLGPVPLFNWMPFQL
CCCCHHHHHHHHCCCEEEEEECCCEEEEEEEEEECCCCCCCCCEEEECCCCHHCCCCHHH
SAGITLFSLFLLSLGVYGLILPLERKIRQVRYALNRMKSGDLSLRVPIEGSDEMANLASS
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHH
YNNMSDHIQRLIEAQRELMRAVSHELRTPVARIRFGMEMLAEEDDYNYRIQQMEMIDRDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
EALNTLIDEIMTYAKLEQGTPSLDFEEIILVDLLNQVAIETEALKTQKEIELIPPPTYVM
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHCEEEECCCCEEE
VDAERRYLHRVVQNLVGNAVRYCNDKVRISGGIHPDGHAFICVEDDGAGIAEEDRARVFE
EECHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHH
AFARLDDSRTRASGGYGLGLSIVSRIAYWFGGTIQVDESPELGGARFVMQWPAKRFNENS
HHHHCCCCHHCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEECCHHHCCCCC
KKKKSI
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]