Click here to switch to the map view.

The map label for this gene is rpoN [H]

Identifier: 50083887

GI number: 50083887

Start: 651692

End: 653140

Strand: Direct

Name: rpoN [H]

Synonym: ACIAD0657

Alternate gene names: 50083887

Gene position: 651692-653140 (Clockwise)

Preceding gene: 50083883

Following gene: 50083888

Centisome position: 18.11

GC content: 37.06

Gene sequence:

>1449_bases
ATGAAGTTATCTGTTGGATTGAAAGTCGCGAATTCTTTATCGCTTACCCCTCAATTACAACAAGCGATTCGTTTATTGCA
ATTATCAAGTCTGGAGCTTGAACAAGAAATCCAGATTCAATTAGATAGTAATCCATTACTTGAAAAAATAGAAGATGAGG
GTACAGCAGAAAGTTTAACCCATTTAGAAAATAAAGAAAGCCGAGATCTAACTCAGGATTTAAATGCGGACCATTTACCC
AATGATCTTCCTGTGGATACCGATTGGGATGATGTGTATACGCATCAATCTACAGCTTTGGCTTCACCAGAATATGAAGA
ACGGGAAGATAATCGTCAAGCTGAGCATACTTTAAAAGAACATATTTTGGCCCAAGTAAATTTATTGCATTTTTCGACTG
TCGATCGGTTAATTGCTTATTGCGTTGTAGATTGTCTGGATGAGAAAGGTTTTCTAGATGCCGAAATTGAAGAAATTATT
GAAGCGGCACAGTATTTATTGTCAGAAGCAGGGCTTGAAGAAGAGATTGAGGCAGATGAGGTGATGGTTGTGCTTAAGCA
TATCCAGCGTCTTGATCCAGTTGGTGTAGGTTCAAAGAATCTTGCAGAATGTTTGAAAGTTCAATTGGATGTTCTTGATG
TTTTTACACCTTTAAGAAATGAAGCTTTAAGTTTATTAAAATATTATGAACTGCTTGTGAGTAACGATTTAAATAAATTA
TTAAAATCTACAAATTTGACCATGTTGCAAGTAAAAGAGGCAATTGAGCTGCTTAAAACATTAAAGCCTTATCCAGGCTT
GGACTTTGAAGCACAGGATTCAGATTATCAAGTTCCTGATGTGGTGGTCACTAAAAAAGATCAATCTTGGCAGGTACAGC
TTAATCCTGACATTATGCCCAAATTACGTATCAATTCATTCTATTCTAATATGATTCGTAGAGCAGATCAGAGTGATGGA
AACATGTATCTGCGCAATCAGATGCTGGAAGCCAAAAATTTTATAAAAAGCATTGATGAACGTCATAAAACATTATTAAA
AGTTGCAACCTGTATTGTTGAACATCAACGTAATTTTTTCGAAATTGGTCCCGAGGGTATGAAACCTTTGGTACTGCGTG
ATGTGGCAGAGGAAGTTGAGCTGCATGAGTCAACAGTGTCGCGGGTAACCACCAATAAATATATGCTGACTCCACGTGGC
TTATTCGAATTGAAATACTTTTTTTCAAGTCATGTCGGAACAACCTCTGGAGGAGAGTGTTCTTCAACTGCGATTCGTGC
GCGTATTAAAAAAATGATTTCTGCGGAAAATCCACGTAAACCTTTGTCGGATAATATTATTGCAAATATGCTCAAAGATG
AAGGAATTGATGTAGCGCGTAGAACCGTTGCAAAATATCGTGAATCGTTACATATCCCCTCATCTTCTGAAAGAAAAGTC
TTGATCTGA

Upstream 100 bases:

>100_bases
CTGAAATATTTTTTTTTGTAGGATAAAGTTAAATCCTATGCTATAACAATGATATCGGCATATTTTTTGCATTGTATAAT
AGTAGTCAGGATATTGCTTT

Downstream 100 bases:

>100_bases
TCTGCATAATCTGTCTATTCTAGAGATTCATTGTTGCAAAATAATGAATCTTTTTTTTCATCGAAGGAATAGTTTAAATG
ATTTGACGATTGCATTTTAT

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKLSVGLKVANSLSLTPQLQQAIRLLQLSSLELEQEIQIQLDSNPLLEKIEDEGTAESLTHLENKESRDLTQDLNADHLP
NDLPVDTDWDDVYTHQSTALASPEYEEREDNRQAEHTLKEHILAQVNLLHFSTVDRLIAYCVVDCLDEKGFLDAEIEEII
EAAQYLLSEAGLEEEIEADEVMVVLKHIQRLDPVGVGSKNLAECLKVQLDVLDVFTPLRNEALSLLKYYELLVSNDLNKL
LKSTNLTMLQVKEAIELLKTLKPYPGLDFEAQDSDYQVPDVVVTKKDQSWQVQLNPDIMPKLRINSFYSNMIRRADQSDG
NMYLRNQMLEAKNFIKSIDERHKTLLKVATCIVEHQRNFFEIGPEGMKPLVLRDVAEEVELHESTVSRVTTNKYMLTPRG
LFELKYFFSSHVGTTSGGECSSTAIRARIKKMISAENPRKPLSDNIIANMLKDEGIDVARRTVAKYRESLHIPSSSERKV
LI

Sequences:

>Translated_482_residues
MKLSVGLKVANSLSLTPQLQQAIRLLQLSSLELEQEIQIQLDSNPLLEKIEDEGTAESLTHLENKESRDLTQDLNADHLP
NDLPVDTDWDDVYTHQSTALASPEYEEREDNRQAEHTLKEHILAQVNLLHFSTVDRLIAYCVVDCLDEKGFLDAEIEEII
EAAQYLLSEAGLEEEIEADEVMVVLKHIQRLDPVGVGSKNLAECLKVQLDVLDVFTPLRNEALSLLKYYELLVSNDLNKL
LKSTNLTMLQVKEAIELLKTLKPYPGLDFEAQDSDYQVPDVVVTKKDQSWQVQLNPDIMPKLRINSFYSNMIRRADQSDG
NMYLRNQMLEAKNFIKSIDERHKTLLKVATCIVEHQRNFFEIGPEGMKPLVLRDVAEEVELHESTVSRVTTNKYMLTPRG
LFELKYFFSSHVGTTSGGECSSTAIRARIKKMISAENPRKPLSDNIIANMLKDEGIDVARRTVAKYRESLHIPSSSERKV
LI
>Mature_482_residues
MKLSVGLKVANSLSLTPQLQQAIRLLQLSSLELEQEIQIQLDSNPLLEKIEDEGTAESLTHLENKESRDLTQDLNADHLP
NDLPVDTDWDDVYTHQSTALASPEYEEREDNRQAEHTLKEHILAQVNLLHFSTVDRLIAYCVVDCLDEKGFLDAEIEEII
EAAQYLLSEAGLEEEIEADEVMVVLKHIQRLDPVGVGSKNLAECLKVQLDVLDVFTPLRNEALSLLKYYELLVSNDLNKL
LKSTNLTMLQVKEAIELLKTLKPYPGLDFEAQDSDYQVPDVVVTKKDQSWQVQLNPDIMPKLRINSFYSNMIRRADQSDG
NMYLRNQMLEAKNFIKSIDERHKTLLKVATCIVEHQRNFFEIGPEGMKPLVLRDVAEEVELHESTVSRVTTNKYMLTPRG
LFELKYFFSSHVGTTSGGECSSTAIRARIKKMISAENPRKPLSDNIIANMLKDEGIDVARRTVAKYRESLHIPSSSERKV
LI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=488, Percent_Identity=47.1311475409836, Blast_Score=435, Evalue=1e-123,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 54815; Mature: 54815

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSVGLKVANSLSLTPQLQQAIRLLQLSSLELEQEIQIQLDSNPLLEKIEDEGTAESLT
CCEECCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCHHHHHH
HLENKESRDLTQDLNADHLPNDLPVDTDWDDVYTHQSTALASPEYEEREDNRQAEHTLKE
HHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHH
HILAQVNLLHFSTVDRLIAYCVVDCLDEKGFLDAEIEEIIEAAQYLLSEAGLEEEIEADE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
VMVVLKHIQRLDPVGVGSKNLAECLKVQLDVLDVFTPLRNEALSLLKYYELLVSNDLNKL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKSTNLTMLQVKEAIELLKTLKPYPGLDFEAQDSDYQVPDVVVTKKDQSWQVQLNPDIMP
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCC
KLRINSFYSNMIRRADQSDGNMYLRNQMLEAKNFIKSIDERHKTLLKVATCIVEHQRNFF
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
EIGPEGMKPLVLRDVAEEVELHESTVSRVTTNKYMLTPRGLFELKYFFSSHVGTTSGGEC
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCCCCCCH
SSTAIRARIKKMISAENPRKPLSDNIIANMLKDEGIDVARRTVAKYRESLHIPSSSERKV
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCC
LI
CC
>Mature Secondary Structure
MKLSVGLKVANSLSLTPQLQQAIRLLQLSSLELEQEIQIQLDSNPLLEKIEDEGTAESLT
CCEECCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCHHHHHH
HLENKESRDLTQDLNADHLPNDLPVDTDWDDVYTHQSTALASPEYEEREDNRQAEHTLKE
HHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHH
HILAQVNLLHFSTVDRLIAYCVVDCLDEKGFLDAEIEEIIEAAQYLLSEAGLEEEIEADE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
VMVVLKHIQRLDPVGVGSKNLAECLKVQLDVLDVFTPLRNEALSLLKYYELLVSNDLNKL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKSTNLTMLQVKEAIELLKTLKPYPGLDFEAQDSDYQVPDVVVTKKDQSWQVQLNPDIMP
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCC
KLRINSFYSNMIRRADQSDGNMYLRNQMLEAKNFIKSIDERHKTLLKVATCIVEHQRNFF
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
EIGPEGMKPLVLRDVAEEVELHESTVSRVTTNKYMLTPRGLFELKYFFSSHVGTTSGGEC
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCCCCCCH
SSTAIRARIKKMISAENPRKPLSDNIIANMLKDEGIDVARRTVAKYRESLHIPSSSERKV
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCC
LI
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8206826 [H]