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The map label for this gene is yfiN [H]

Identifier: 50083885

GI number: 50083885

Start: 643787

End: 645004

Strand: Reverse

Name: yfiN [H]

Synonym: ACIAD0654

Alternate gene names: 50083885

Gene position: 645004-643787 (Counterclockwise)

Preceding gene: 50083886

Following gene: 50083884

Centisome position: 17.92

GC content: 33.42

Gene sequence:

>1218_bases
ATGATTGCGAATTTATATAAATCAAATTCACTGCATAGCGTTTTTCGCAAGTCTCAGGCTACCATTTTTATTATTACTTT
AGTTATTTGCTCTTTTACTTTTGTTTCTATTTCAACCTATACCATGGAAACCTATACCAAACAGAATTTGAATGTTTTAA
GTCTTGCGGTGAGTGAACGGATACAACCTGCTGTTGTGTTTAAAGATCAGCAAACCATTGAACAAATTTTAAATGAATAT
ACGTTTCAACATTCTATTCGTAGCATCAGTGTATATGATAGCCATCAAAATAGGCTTGCTTATAGTTTTAAAAAGACAGA
CTACTATTCTAAATTACAGATATTACTCGATCATTTTTTTCTAAAAGATCCGATAAAACTTAATATTTATCATAATAATA
AACAAGTCGGTGAACTTGTTGTTTTTGGAAGTTCAGAAAAAATCTTACAATTTATTCTCACCATCCTGATTGGTCTAGCT
GTGGTGATGCTGTTTATGATATTAGCATTATGGTGGTCCACCAATTCTACTTATCGCTACATTGTACAGTCTATGCAGCC
ACTAACCCAGACAGCACAACTTGTTAGTCAACAAAAAGCATATAATCTTCGTTTTCCAGACAATAACATTAAAGAATTTC
AAGATCTTAATGATGTATTTAATGAGCTACTACAGGAAATTCAGACTTGGCATAACCACTTACAGCATGAAAATCATTTG
CTATCGTTTGAGGCAAAACATGACCATCTCACCGCCCTTCCAAATCGAAGCTATTTTTATACACAATTAATGCATGTTTT
TGAAAATCCAAATTCACGAAAAACGTCTGCATTAATTTTCATTGATAATAATAATTTTAAATATATTAACGATCAATATG
GGCATCTTGCTGGAGACGCTGTGCTACAAGAAATGGCAAATCGACTGAAACAACGATTACGTCATCAGGACTTTATTGCT
CGACTGGGTGGAGATGAATTTGCAGTGATTTTAGAATCTGTAACTCAAGTGAATCACCTGATTACAATAGCAGAAAACTT
ACTCAACAGTTGCCAACAACCTCTTAATTTTAATGGTCAATCTATACATTTTAGTTTTAGTTTAGGGATTGCATTTTCTC
AATTTGCTTCCACTCCTGAAGAACTAATTGCTCAAGCAGATCAAGCCATGTATAAGGCAAAACATCTCACCCATCACTGG
TTTATCCATAAACCGTAA

Upstream 100 bases:

>100_bases
AAACGCTACATTTAAGGTCAATTTAGATAGTTTAAGCCGCTCTCAAATTCGCATTGATCCTCGTGTCTTACTTTTAGCAA
AAAATGCGGAGTAACTTGTC

Downstream 100 bases:

>100_bases
ATTTCAGGTAGTTCACTATGTATAACAACTCAATCAAAATATCTATTCTTGGGGTCATTTGCCTTATTCTGGCAGGATGT
CTAAGCTTTGGACCACTCAA

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MIANLYKSNSLHSVFRKSQATIFIITLVICSFTFVSISTYTMETYTKQNLNVLSLAVSERIQPAVVFKDQQTIEQILNEY
TFQHSIRSISVYDSHQNRLAYSFKKTDYYSKLQILLDHFFLKDPIKLNIYHNNKQVGELVVFGSSEKILQFILTILIGLA
VVMLFMILALWWSTNSTYRYIVQSMQPLTQTAQLVSQQKAYNLRFPDNNIKEFQDLNDVFNELLQEIQTWHNHLQHENHL
LSFEAKHDHLTALPNRSYFYTQLMHVFENPNSRKTSALIFIDNNNFKYINDQYGHLAGDAVLQEMANRLKQRLRHQDFIA
RLGGDEFAVILESVTQVNHLITIAENLLNSCQQPLNFNGQSIHFSFSLGIAFSQFASTPEELIAQADQAMYKAKHLTHHW
FIHKP

Sequences:

>Translated_405_residues
MIANLYKSNSLHSVFRKSQATIFIITLVICSFTFVSISTYTMETYTKQNLNVLSLAVSERIQPAVVFKDQQTIEQILNEY
TFQHSIRSISVYDSHQNRLAYSFKKTDYYSKLQILLDHFFLKDPIKLNIYHNNKQVGELVVFGSSEKILQFILTILIGLA
VVMLFMILALWWSTNSTYRYIVQSMQPLTQTAQLVSQQKAYNLRFPDNNIKEFQDLNDVFNELLQEIQTWHNHLQHENHL
LSFEAKHDHLTALPNRSYFYTQLMHVFENPNSRKTSALIFIDNNNFKYINDQYGHLAGDAVLQEMANRLKQRLRHQDFIA
RLGGDEFAVILESVTQVNHLITIAENLLNSCQQPLNFNGQSIHFSFSLGIAFSQFASTPEELIAQADQAMYKAKHLTHHW
FIHKP
>Mature_405_residues
MIANLYKSNSLHSVFRKSQATIFIITLVICSFTFVSISTYTMETYTKQNLNVLSLAVSERIQPAVVFKDQQTIEQILNEY
TFQHSIRSISVYDSHQNRLAYSFKKTDYYSKLQILLDHFFLKDPIKLNIYHNNKQVGELVVFGSSEKILQFILTILIGLA
VVMLFMILALWWSTNSTYRYIVQSMQPLTQTAQLVSQQKAYNLRFPDNNIKEFQDLNDVFNELLQEIQTWHNHLQHENHL
LSFEAKHDHLTALPNRSYFYTQLMHVFENPNSRKTSALIFIDNNNFKYINDQYGHLAGDAVLQEMANRLKQRLRHQDFIA
RLGGDEFAVILESVTQVNHLITIAENLLNSCQQPLNFNGQSIHFSFSLGIAFSQFASTPEELIAQADQAMYKAKHLTHHW
FIHKP

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1788956, Length=399, Percent_Identity=30.8270676691729, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1788381, Length=158, Percent_Identity=37.3417721518987, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787541, Length=152, Percent_Identity=34.8684210526316, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081881, Length=159, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786584, Length=166, Percent_Identity=31.9277108433735, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI145693134, Length=155, Percent_Identity=32.258064516129, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787802, Length=163, Percent_Identity=28.8343558282209, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787262, Length=156, Percent_Identity=30.7692307692308, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=34.5454545454545, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787056, Length=148, Percent_Identity=35.8108108108108, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=30.6748466257669, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI87081977, Length=178, Percent_Identity=31.4606741573034, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081974, Length=156, Percent_Identity=30.1282051282051, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 47136; Mature: 47136

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIANLYKSNSLHSVFRKSQATIFIITLVICSFTFVSISTYTMETYTKQNLNVLSLAVSER
CCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEHHHHHCCCHHHHHHHHHC
IQPAVVFKDQQTIEQILNEYTFQHSIRSISVYDSHQNRLAYSFKKTDYYSKLQILLDHFF
CCCEEEECCHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHH
LKDPIKLNIYHNNKQVGELVVFGSSEKILQFILTILIGLAVVMLFMILALWWSTNSTYRY
HCCCEEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
IVQSMQPLTQTAQLVSQQKAYNLRFPDNNIKEFQDLNDVFNELLQEIQTWHNHLQHENHL
HHHHHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LSFEAKHDHLTALPNRSYFYTQLMHVFENPNSRKTSALIFIDNNNFKYINDQYGHLAGDA
EEEECCCCCEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEECCCCCHHHHHH
VLQEMANRLKQRLRHQDFIARLGGDEFAVILESVTQVNHLITIAENLLNSCQQPLNFNGQ
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SIHFSFSLGIAFSQFASTPEELIAQADQAMYKAKHLTHHWFIHKP
EEEEEEEECHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEECC
>Mature Secondary Structure
MIANLYKSNSLHSVFRKSQATIFIITLVICSFTFVSISTYTMETYTKQNLNVLSLAVSER
CCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEHHHHHCCCHHHHHHHHHC
IQPAVVFKDQQTIEQILNEYTFQHSIRSISVYDSHQNRLAYSFKKTDYYSKLQILLDHFF
CCCEEEECCHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHH
LKDPIKLNIYHNNKQVGELVVFGSSEKILQFILTILIGLAVVMLFMILALWWSTNSTYRY
HCCCEEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
IVQSMQPLTQTAQLVSQQKAYNLRFPDNNIKEFQDLNDVFNELLQEIQTWHNHLQHENHL
HHHHHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LSFEAKHDHLTALPNRSYFYTQLMHVFENPNSRKTSALIFIDNNNFKYINDQYGHLAGDA
EEEECCCCCEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEECCCCCHHHHHH
VLQEMANRLKQRLRHQDFIARLGGDEFAVILESVTQVNHLITIAENLLNSCQQPLNFNGQ
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SIHFSFSLGIAFSQFASTPEELIAQADQAMYKAKHLTHHWFIHKP
EEEEEEEECHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 6357787; 7567469 [H]