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The map label for this gene is trpA

Identifier: 50083874

GI number: 50083874

Start: 630720

End: 631523

Strand: Direct

Name: trpA

Synonym: ACIAD0642

Alternate gene names: 50083874

Gene position: 630720-631523 (Clockwise)

Preceding gene: 50083873

Following gene: 50083875

Centisome position: 17.53

GC content: 47.64

Gene sequence:

>804_bases
ATGTCACGTCTTGCCACCCGTTTTGAACAGCTTAAATCCCAACAACGTAAAGCACTGGTTTCATATGTGATGGCAGGTGA
TCCACAGCCGCAAGTGTCTGTACCATTGTTGCACAAGATGGTAGAGGCGGGTGTAGATGTTATTGAGCTCGGTCTGCCTT
TTTCAGACCCGATGGCAGATGGTCCAGTGATTGCGCTTGCAGCCGAACGAGCTCTGGCTGCTGGAACCAATACCCTCGAT
GCACTGAATATGGTGAAGGAGTTTCGTCAGAACGATAGCGAAACACCTGTGGTTCTGATGGGCTATCTGAATCCTGTTGA
AGTGATCGGTTACGAAAAATTTGTATCTCATGCTAAGGCATGTGGCGTAGATGGTGTTCTTTTAGTGGATTTACCACCAG
AAGAAGCAAAAGAATTTGATGTGGTGCTTAAGCAGCATGATATGGATCAGATCTTTTTACTTGCACCGACTTCGACAGAC
CAGCGAATCGAGCACGTAGTGAAGCAGGCCAGTGGTTTTATTTACTATGTTTCACTCAAGGGTGTGACTGGGGCCGCAAC
ATTGGACGTGACTGAAGCGGCGGAACGTATCGCCAAGATTAAAACCAAAACGTCCGTACCTGTTGGTGTCGGTTTTGGGA
TCAGTGATGCAGCATCGGCCAAAGCGATGGGTCAGGTTGCAGATGCAGTGATTGTAGGAAGTGCTTTCGTTAAACCTTTT
GCTACGTTAGCAATCGACCAAGCGGTTGAACAAACGGTCAATAAAGTCAAGGAGCTTCGAGCAGCGCTCGATGAGTTAGT
ATGA

Upstream 100 bases:

>100_bases
CAATGAGCAAAAAATAGCGTTAAAATGCGATGTCTTGTATTTCTACAAACAACTCCCTTTTTTAAATTAGGTTTTAAATA
ATCAAGGAAAGCACAACCCT

Downstream 100 bases:

>100_bases
ATCAAGAAGTGAAATCAGGTAAAATCCTAAGCCCATCGACGCCATGGACTGAACGTCAGGTACCAGGAATACAAGTTGCC
AATGAACAACAATCGCTCAA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSRLATRFEQLKSQQRKALVSYVMAGDPQPQVSVPLLHKMVEAGVDVIELGLPFSDPMADGPVIALAAERALAAGTNTLD
ALNMVKEFRQNDSETPVVLMGYLNPVEVIGYEKFVSHAKACGVDGVLLVDLPPEEAKEFDVVLKQHDMDQIFLLAPTSTD
QRIEHVVKQASGFIYYVSLKGVTGAATLDVTEAAERIAKIKTKTSVPVGVGFGISDAASAKAMGQVADAVIVGSAFVKPF
ATLAIDQAVEQTVNKVKELRAALDELV

Sequences:

>Translated_267_residues
MSRLATRFEQLKSQQRKALVSYVMAGDPQPQVSVPLLHKMVEAGVDVIELGLPFSDPMADGPVIALAAERALAAGTNTLD
ALNMVKEFRQNDSETPVVLMGYLNPVEVIGYEKFVSHAKACGVDGVLLVDLPPEEAKEFDVVLKQHDMDQIFLLAPTSTD
QRIEHVVKQASGFIYYVSLKGVTGAATLDVTEAAERIAKIKTKTSVPVGVGFGISDAASAKAMGQVADAVIVGSAFVKPF
ATLAIDQAVEQTVNKVKELRAALDELV
>Mature_266_residues
SRLATRFEQLKSQQRKALVSYVMAGDPQPQVSVPLLHKMVEAGVDVIELGLPFSDPMADGPVIALAAERALAAGTNTLDA
LNMVKEFRQNDSETPVVLMGYLNPVEVIGYEKFVSHAKACGVDGVLLVDLPPEEAKEFDVVLKQHDMDQIFLLAPTSTDQ
RIEHVVKQASGFIYYVSLKGVTGAATLDVTEAAERIAKIKTKTSVPVGVGFGISDAASAKAMGQVADAVIVGSAFVKPFA
TLAIDQAVEQTVNKVKELRAALDELV

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=268, Percent_Identity=34.7014925373134, Blast_Score=149, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6321412, Length=236, Percent_Identity=38.9830508474576, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_ACIAD (Q6FEE6)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045384.1
- ProteinModelPortal:   Q6FEE6
- SMR:   Q6FEE6
- STRING:   Q6FEE6
- GeneID:   2880733
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0642
- NMPDR:   fig|62977.3.peg.287
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   TSDERIR
- PhylomeDB:   Q6FEE6
- ProtClustDB:   PRK13111
- BioCyc:   ASP62977:ACIAD0642-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28549; Mature: 28418

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLATRFEQLKSQQRKALVSYVMAGDPQPQVSVPLLHKMVEAGVDVIELGLPFSDPMAD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIALAAERALAAGTNTLDALNMVKEFRQNDSETPVVLMGYLNPVEVIGYEKFVSHAKA
CCEEEEEHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
CGVDGVLLVDLPPEEAKEFDVVLKQHDMDQIFLLAPTSTDQRIEHVVKQASGFIYYVSLK
CCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCEEEEEEEC
GVTGAATLDVTEAAERIAKIKTKTSVPVGVGFGISDAASAKAMGQVADAVIVGSAFVKPF
CCCCCCEEHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ATLAIDQAVEQTVNKVKELRAALDELV
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRLATRFEQLKSQQRKALVSYVMAGDPQPQVSVPLLHKMVEAGVDVIELGLPFSDPMAD
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIALAAERALAAGTNTLDALNMVKEFRQNDSETPVVLMGYLNPVEVIGYEKFVSHAKA
CCEEEEEHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
CGVDGVLLVDLPPEEAKEFDVVLKQHDMDQIFLLAPTSTDQRIEHVVKQASGFIYYVSLK
CCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCEEEEEEEC
GVTGAATLDVTEAAERIAKIKTKTSVPVGVGFGISDAASAKAMGQVADAVIVGSAFVKPF
CCCCCCEEHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ATLAIDQAVEQTVNKVKELRAALDELV
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA