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The map label for this gene is trpF [H]

Identifier: 50083868

GI number: 50083868

Start: 624670

End: 625344

Strand: Direct

Name: trpF [H]

Synonym: ACIAD0635

Alternate gene names: 50083868

Gene position: 624670-625344 (Clockwise)

Preceding gene: 50083864

Following gene: 50083869

Centisome position: 17.36

GC content: 45.93

Gene sequence:

>675_bases
CTGTCTGTCAGGCAATACAATGAGCTTCTTTCTATGCGAACGCGCGCAAAAATTTGCGGTATTACCCGTTCCCAAGATGT
CCAAGCAGCAGTAAGTGCAGGTGCAGATGCCATTGGACTGGTTTTTTTCCCACCAAGTCCTCGACATGTTTCTATAGCGC
AAGCGCAAGCATTGCTCCAGCATATTCCCGCTTATGTTCAGGTGGTTGGTTTATTTGTGAATGCAACTGCGGATCAAATC
AAATCAGTGCTTGATTGTGTGGCTTTGGATGTATTACAACTACATGGCGATGAAACGCCTGAGCAATGTCAAGAGATTGC
TCTGCAGTGCAAGCGTCGCTGGTATAAAGCCATTCAAGTTAAACCAGAGCTTGATGTAGTTGATGAAGTTCAGCGTTATC
AGGCCGCTGGTGCAAGTGCGGTATTGCTGGATGCGTGGCATCCAGAGCTCAAAGGTGGAACTGGTCATCAATTTGATTGG
TCGAAGTTTCCCAAGCTGGATATTCCACTTATTCTTGCAGGCGGTTTAACGCCTGAAAATGTTGTAGATGCCATTCAAAC
CACACACGCTTTTGCAGTGGATGTGAGCGGAGGGGTAGAGGCCGCAAAAGGTATTAAAGATAAACAACTCATCGAACGAT
TTATGCAAGGAGTCCAATGTGGATCAGCAAAATAA

Upstream 100 bases:

>100_bases
AGCGAAGATTAAACAATCTGAATACGATCAAATTCGTTCAACTTTGACGCAAAGCACAAAAATTGCATTACAATACTTAG
CCCAATGATGGATAGATCGG

Downstream 100 bases:

>100_bases
CGTGATTGACTATACGCAATATCCAGATGCTCGTGGGCATTTTGGTATTCATGGCGGACGTTTTGTATCAGAAACACTTA
TGGCGGCACTTGAAGATTTA

Product: N-(5'-phosphoribosyl)anthranilate isomerase (PRAI)

Products: NA

Alternate protein names: PRAI [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MSVRQYNELLSMRTRAKICGITRSQDVQAAVSAGADAIGLVFFPPSPRHVSIAQAQALLQHIPAYVQVVGLFVNATADQI
KSVLDCVALDVLQLHGDETPEQCQEIALQCKRRWYKAIQVKPELDVVDEVQRYQAAGASAVLLDAWHPELKGGTGHQFDW
SKFPKLDIPLILAGGLTPENVVDAIQTTHAFAVDVSGGVEAAKGIKDKQLIERFMQGVQCGSAK

Sequences:

>Translated_224_residues
MSVRQYNELLSMRTRAKICGITRSQDVQAAVSAGADAIGLVFFPPSPRHVSIAQAQALLQHIPAYVQVVGLFVNATADQI
KSVLDCVALDVLQLHGDETPEQCQEIALQCKRRWYKAIQVKPELDVVDEVQRYQAAGASAVLLDAWHPELKGGTGHQFDW
SKFPKLDIPLILAGGLTPENVVDAIQTTHAFAVDVSGGVEAAKGIKDKQLIERFMQGVQCGSAK
>Mature_223_residues
SVRQYNELLSMRTRAKICGITRSQDVQAAVSAGADAIGLVFFPPSPRHVSIAQAQALLQHIPAYVQVVGLFVNATADQIK
SVLDCVALDVLQLHGDETPEQCQEIALQCKRRWYKAIQVKPELDVVDEVQRYQAAGASAVLLDAWHPELKGGTGHQFDWS
KFPKLDIPLILAGGLTPENVVDAIQTTHAFAVDVSGGVEAAKGIKDKQLIERFMQGVQCGSAK

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family [H]

Homologues:

Organism=Escherichia coli, GI87081863, Length=208, Percent_Identity=34.1346153846154, Blast_Score=93, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320210, Length=219, Percent_Identity=28.7671232876712, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00697 PRAI [H]

EC number: =5.3.1.24 [H]

Molecular weight: Translated: 24327; Mature: 24196

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRQYNELLSMRTRAKICGITRSQDVQAAVSAGADAIGLVFFPPSPRHVSIAQAQALLQ
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCCEEHHHHHHHHHH
HIPAYVQVVGLFVNATADQIKSVLDCVALDVLQLHGDETPEQCQEIALQCKRRWYKAIQV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
KPELDVVDEVQRYQAAGASAVLLDAWHPELKGGTGHQFDWSKFPKLDIPLILAGGLTPEN
CCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHH
VVDAIQTTHAFAVDVSGGVEAAKGIKDKQLIERFMQGVQCGSAK
HHHHHHHHHHEEEECCCCHHHHHCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SVRQYNELLSMRTRAKICGITRSQDVQAAVSAGADAIGLVFFPPSPRHVSIAQAQALLQ
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCCEEHHHHHHHHHH
HIPAYVQVVGLFVNATADQIKSVLDCVALDVLQLHGDETPEQCQEIALQCKRRWYKAIQV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
KPELDVVDEVQRYQAAGASAVLLDAWHPELKGGTGHQFDWSKFPKLDIPLILAGGLTPEN
CCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHH
VVDAIQTTHAFAVDVSGGVEAAKGIKDKQLIERFMQGVQCGSAK
HHHHHHHHHHEEEECCCCHHHHHCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2299982; 2211532 [H]