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The map label for this gene is rlmN

Identifier: 50083794

GI number: 50083794

Start: 549209

End: 550453

Strand: Direct

Name: rlmN

Synonym: ACIAD0557

Alternate gene names: 50083794

Gene position: 549209-550453 (Clockwise)

Preceding gene: 50083793

Following gene: 50083795

Centisome position: 15.26

GC content: 43.21

Gene sequence:

>1245_bases
ATGACTTCTGCAGTGGGCATTTCAGTGCCAAATACAGACGCACAATCCTCTCAATCTGCATCTCAAACGACTGTAGAGCA
GGAAGTCTTGAAAAGTAAAGTTAACCTGTTGGGAATGTCGCGGATAGAGCTTGAACAATTTTTTGAGCAAATTGGAGAGA
AAAAATTTCGTGCTGGCCAGATCATGAAATGGATCCATCAATATTTTGTGACTGACCTTGCTGAAATGACCAACATATCA
GGTAAGTTGCGTACAAAACTTGAGCAAGTTTGCGAAATTAAAGCGCCTGAAGTTGTACATCGTCATTATTCAAAAGATGG
TACACGTAAATGGGTTTTTCGGGTCGGTGAAGGAAGTGGTTCTTTGGTAGAAACCGTTTTGATTCCTGCCGAAGACAAAA
CAGGTTCACGCAAAACGCTATGCATTTCTTCGCAAGTCGGTTGTGCTTTGGATTGCTCATTTTGTTCGACAGGCAAGCAA
GGTTTTCAGCGTGACTTGACACCTGCCGAAATTATCGGGCAGTTGTGGGTGGCTAACCAGTCTTATGTTGAAGATGTACC
TGTGGCAGAACGTACCCGTGCAGTGACCAATGTGGTGATGATGGGGATGGGTGAGCCTCTACTTAATTTCAAACCAGTCG
TACATTCCATGTCAATTATGCTCGATGATTATGCTTATGGCATGTCGAAACGTCGTGTGACGTTATCAACATCGGGTGTG
GTGCCTATGATTGATAAACTTGCAGAAGAGTTAGATGTCGCCCTCGCAATTTCATTACATGCACCGAATAATCCTTTACG
TGATGAACTGGTGCCAATCAATAAGAAATATCCGCTTGAGCAGTTGATTGCAGCAGCGCAGCGTTACATTACCAAAGATG
GTAATGAGAGTGCCCGTAAGCATGTCACGATTGAGTATGTCATGTTAGATGGCGTGAATGATCATCCAGAACATGCTCAA
CAGCTCGTCAAATTATTAAAAAATTTACCAAGTAAAATTAACTTGATTCCATTTAATCCATTTCCGCATGCACCTTATGG
ACGTTCAAGCCGTAATCGTATCATGGCATTTCAAAAGACTTTGTCTGATGCTGGATTTGTGTGTACGATTCGTCAGACAC
GTGGTGATGATATCGATGCAGCCTGTGGACAGTTGGTGGGTCAAGTCGCAGATCGGACACGTCGTGCAGAACAATGGAAA
AAGAAAGTTGCTGAGCGACAAGAGATTATTCGCTCGCAAGGATAA

Upstream 100 bases:

>100_bases
AGTGTTATTATACTTATCTGGTTTTTTTATTCTCTCTTGATGTGATTGCTTGTTTTTTAGGCAGTCTGATTTCATCTCAA
TTTTTGGTTTAGGTAATTAC

Downstream 100 bases:

>100_bases
TTATCTGGGGGTGGTGGGTTGAGCAAGTGGACAATAAACAAAACGTTGGGTTGTGCCGCCATTGTAGGGGCGTTTATTTT
GTCTGGATGTCAGACTGTTC

Product: Fe-S-cluster redox enzyme

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MTSAVGISVPNTDAQSSQSASQTTVEQEVLKSKVNLLGMSRIELEQFFEQIGEKKFRAGQIMKWIHQYFVTDLAEMTNIS
GKLRTKLEQVCEIKAPEVVHRHYSKDGTRKWVFRVGEGSGSLVETVLIPAEDKTGSRKTLCISSQVGCALDCSFCSTGKQ
GFQRDLTPAEIIGQLWVANQSYVEDVPVAERTRAVTNVVMMGMGEPLLNFKPVVHSMSIMLDDYAYGMSKRRVTLSTSGV
VPMIDKLAEELDVALAISLHAPNNPLRDELVPINKKYPLEQLIAAAQRYITKDGNESARKHVTIEYVMLDGVNDHPEHAQ
QLVKLLKNLPSKINLIPFNPFPHAPYGRSSRNRIMAFQKTLSDAGFVCTIRQTRGDDIDAACGQLVGQVADRTRRAEQWK
KKVAERQEIIRSQG

Sequences:

>Translated_414_residues
MTSAVGISVPNTDAQSSQSASQTTVEQEVLKSKVNLLGMSRIELEQFFEQIGEKKFRAGQIMKWIHQYFVTDLAEMTNIS
GKLRTKLEQVCEIKAPEVVHRHYSKDGTRKWVFRVGEGSGSLVETVLIPAEDKTGSRKTLCISSQVGCALDCSFCSTGKQ
GFQRDLTPAEIIGQLWVANQSYVEDVPVAERTRAVTNVVMMGMGEPLLNFKPVVHSMSIMLDDYAYGMSKRRVTLSTSGV
VPMIDKLAEELDVALAISLHAPNNPLRDELVPINKKYPLEQLIAAAQRYITKDGNESARKHVTIEYVMLDGVNDHPEHAQ
QLVKLLKNLPSKINLIPFNPFPHAPYGRSSRNRIMAFQKTLSDAGFVCTIRQTRGDDIDAACGQLVGQVADRTRRAEQWK
KKVAERQEIIRSQG
>Mature_413_residues
TSAVGISVPNTDAQSSQSASQTTVEQEVLKSKVNLLGMSRIELEQFFEQIGEKKFRAGQIMKWIHQYFVTDLAEMTNISG
KLRTKLEQVCEIKAPEVVHRHYSKDGTRKWVFRVGEGSGSLVETVLIPAEDKTGSRKTLCISSQVGCALDCSFCSTGKQG
FQRDLTPAEIIGQLWVANQSYVEDVPVAERTRAVTNVVMMGMGEPLLNFKPVVHSMSIMLDDYAYGMSKRRVTLSTSGVV
PMIDKLAEELDVALAISLHAPNNPLRDELVPINKKYPLEQLIAAAQRYITKDGNESARKHVTIEYVMLDGVNDHPEHAQQ
LVKLLKNLPSKINLIPFNPFPHAPYGRSSRNRIMAFQKTLSDAGFVCTIRQTRGDDIDAACGQLVGQVADRTRRAEQWKK
KVAERQEIIRSQG

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=365, Percent_Identity=59.1780821917808, Blast_Score=432, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_ACIAD (Q6FEM6)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045304.1
- ProteinModelPortal:   Q6FEM6
- STRING:   Q6FEM6
- GeneID:   2878992
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0557
- NMPDR:   fig|62977.3.peg.43
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   Q6FEM6
- ProtClustDB:   CLSK830487
- BioCyc:   ASP62977:ACIAD0557-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 46162; Mature: 46030

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSAVGISVPNTDAQSSQSASQTTVEQEVLKSKVNLLGMSRIELEQFFEQIGEKKFRAGQ
CCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
IMKWIHQYFVTDLAEMTNISGKLRTKLEQVCEIKAPEVVHRHYSKDGTRKWVFRVGEGSG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCC
SLVETVLIPAEDKTGSRKTLCISSQVGCALDCSFCSTGKQGFQRDLTPAEIIGQLWVANQ
CEEEEEEEECCCCCCCCEEEEEECCCCCEEECHHHHCCHHHHHCCCCHHHHHHHHHHCCC
SYVEDVPVAERTRAVTNVVMMGMGEPLLNFKPVVHSMSIMLDDYAYGMSKRRVTLSTSGV
HHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
VPMIDKLAEELDVALAISLHAPNNPLRDELVPINKKYPLEQLIAAAQRYITKDGNESARK
HHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
HVTIEYVMLDGVNDHPEHAQQLVKLLKNLPSKINLIPFNPFPHAPYGRSSRNRIMAFQKT
EEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHH
LSDAGFVCTIRQTRGDDIDAACGQLVGQVADRTRRAEQWKKKVAERQEIIRSQG
HCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSAVGISVPNTDAQSSQSASQTTVEQEVLKSKVNLLGMSRIELEQFFEQIGEKKFRAGQ
CCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
IMKWIHQYFVTDLAEMTNISGKLRTKLEQVCEIKAPEVVHRHYSKDGTRKWVFRVGEGSG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCC
SLVETVLIPAEDKTGSRKTLCISSQVGCALDCSFCSTGKQGFQRDLTPAEIIGQLWVANQ
CEEEEEEEECCCCCCCCEEEEEECCCCCEEECHHHHCCHHHHHCCCCHHHHHHHHHHCCC
SYVEDVPVAERTRAVTNVVMMGMGEPLLNFKPVVHSMSIMLDDYAYGMSKRRVTLSTSGV
HHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
VPMIDKLAEELDVALAISLHAPNNPLRDELVPINKKYPLEQLIAAAQRYITKDGNESARK
HHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
HVTIEYVMLDGVNDHPEHAQQLVKLLKNLPSKINLIPFNPFPHAPYGRSSRNRIMAFQKT
EEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHH
LSDAGFVCTIRQTRGDDIDAACGQLVGQVADRTRRAEQWKKKVAERQEIIRSQG
HCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA