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The map label for this gene is eda [H]

Identifier: 50083781

GI number: 50083781

Start: 534304

End: 534933

Strand: Direct

Name: eda [H]

Synonym: ACIAD0543

Alternate gene names: 50083781

Gene position: 534304-534933 (Clockwise)

Preceding gene: 50083780

Following gene: 50083782

Centisome position: 14.85

GC content: 48.89

Gene sequence:

>630_bases
ATGATCAAGATGGAAGATGTTGTTCAATTAGGGCCTGTGATTCCTGTACTGGCATTCGATTCGGCGGAACAAGGGGGACA
TGTATCCCGCGCGTTGCACGCTGGTGGTGTGAAAGTCCTTGAAATTACCTTACGTACGGCGGCTGGTCTTGCTGCAATTG
AGCGTGCGAGCCAGTTGGCTGATGACATTGTGGTCGGTGTCGGTACGATTACCAAACCAGAACACTGTGCTCAAGCCAAA
AAAGCAGGTGCACAATTTGGCGTATCTCCTGGTTTAACCAAAGACTTACATTTGGCAGCCCAAGATGCTGGCTTACCACT
GTTACCTGGTGTGATGACCCCAAGTGATTTGATTCAGGCAATTGAGCTTGGCTATGAGATTGTCAAGTTTTTCCCAGCTC
AGCAAGCGGGCGGTGTCGCGATGCTTCAGGCATTTTATGGGCCATTTCCAAATTTACGTTTTTGCCCAACGGGTGGAATT
ACAGCAGAGACCGCTCCAGATTTCTTAAAACAACCCAATGTCGTGTGTGTTGGTGGATCGTGGTTAACACCCAAGAAGTT
GGTCGCCGCCGAAGAGTGGGCGCAAATTACCCGTTTGGCTCAAATCGCCAGTCAATTACGTAATGAATAA

Upstream 100 bases:

>100_bases
GGCTGCACCTGCTGAGCATGGCGCTTCTGTATTTGGTGCCTTGGTCGATGAAAACGTTCAAGTTTAGCGTTGAAGAATAA
ATAATAGGAACATAAAAGTA

Downstream 100 bases:

>100_bases
CTACAATCTCAAAGAAGTTTTCAGGCGTGGTGTCGACGTGATTAGCGGATGTTAGTCACACTTAAATATAAAAAATAGTG
TCTCAACAGCAGGAGTCTAG

Product: bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxyphosphogluconate aldolase

Products: NA

Alternate protein names: KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MIKMEDVVQLGPVIPVLAFDSAEQGGHVSRALHAGGVKVLEITLRTAAGLAAIERASQLADDIVVGVGTITKPEHCAQAK
KAGAQFGVSPGLTKDLHLAAQDAGLPLLPGVMTPSDLIQAIELGYEIVKFFPAQQAGGVAMLQAFYGPFPNLRFCPTGGI
TAETAPDFLKQPNVVCVGGSWLTPKKLVAAEEWAQITRLAQIASQLRNE

Sequences:

>Translated_209_residues
MIKMEDVVQLGPVIPVLAFDSAEQGGHVSRALHAGGVKVLEITLRTAAGLAAIERASQLADDIVVGVGTITKPEHCAQAK
KAGAQFGVSPGLTKDLHLAAQDAGLPLLPGVMTPSDLIQAIELGYEIVKFFPAQQAGGVAMLQAFYGPFPNLRFCPTGGI
TAETAPDFLKQPNVVCVGGSWLTPKKLVAAEEWAQITRLAQIASQLRNE
>Mature_209_residues
MIKMEDVVQLGPVIPVLAFDSAEQGGHVSRALHAGGVKVLEITLRTAAGLAAIERASQLADDIVVGVGTITKPEHCAQAK
KAGAQFGVSPGLTKDLHLAAQDAGLPLLPGVMTPSDLIQAIELGYEIVKFFPAQQAGGVAMLQAFYGPFPNLRFCPTGGI
TAETAPDFLKQPNVVCVGGSWLTPKKLVAAEEWAQITRLAQIASQLRNE

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=178, Percent_Identity=44.9438202247191, Blast_Score=162, Evalue=1e-41,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.2.14 [H]

Molecular weight: Translated: 21946; Mature: 21946

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKMEDVVQLGPVIPVLAFDSAEQGGHVSRALHAGGVKVLEITLRTAAGLAAIERASQLA
CCCHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHH
DDIVVGVGTITKPEHCAQAKKAGAQFGVSPGLTKDLHLAAQDAGLPLLPGVMTPSDLIQA
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHH
IELGYEIVKFFPAQQAGGVAMLQAFYGPFPNLRFCPTGGITAETAPDFLKQPNVVCVGGS
HHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHHCCCEEEECCC
WLTPKKLVAAEEWAQITRLAQIASQLRNE
CCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKMEDVVQLGPVIPVLAFDSAEQGGHVSRALHAGGVKVLEITLRTAAGLAAIERASQLA
CCCHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHH
DDIVVGVGTITKPEHCAQAKKAGAQFGVSPGLTKDLHLAAQDAGLPLLPGVMTPSDLIQA
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHH
IELGYEIVKFFPAQQAGGVAMLQAFYGPFPNLRFCPTGGITAETAPDFLKQPNVVCVGGS
HHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHHCCCEEEECCC
WLTPKKLVAAEEWAQITRLAQIASQLRNE
CCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]