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The map label for this gene is tig

Identifier: 50083771

GI number: 50083771

Start: 520056

End: 521390

Strand: Direct

Name: tig

Synonym: ACIAD0533

Alternate gene names: 50083771

Gene position: 520056-521390 (Clockwise)

Preceding gene: 50083763

Following gene: 50083772

Centisome position: 14.45

GC content: 40.9

Gene sequence:

>1335_bases
ATGCAAGTAACGACTGAAGCGGTTTCGGGCGTTGCCCGTCGTTTAAATGTTTCTGTACCGACGAGCCGTATTAATGAGCA
ATTTGAAGCTCGTTTAAAACGCACTGCCAAAACTGCGAAGATCAACGGCTTCCGTCCTGGAAAAGTGCCATTGTCCCGTG
TACGTTCTGATTTTGGCCCAGGTATTTACCAAGAAGTTGTTAACGACATTATTCGTGATACTGTGTTTGAAGCGATTCAA
CAAGAAAAAATCAATGCTGTGGGTATGCCAAACATTGAAAAAGTAGAACATAAAGACGACGCTTTAGTTTATGAAGCAAC
AGTTGAAGTGTATCCAGAAGTTGAAGTAAAAGCATTTGATACGCTTGAAGTTGAACGTAAGGCTGCTGAAATCAACGATA
AAGACGTTGATGCAATGATCGAAAATCTGCAAAAGCAACGTCAAACTTGGGCTGTAACGAAAGGTATGGCCAAAAAAGAC
ATGCAAGTCACGTTTGACTTTGAAGGTGAAGTTGACGGTGAAAAGTTTGAAGGCGGTTCTGCTGAAGACTTTAAACTGGT
ACTTGGTTCTGGTCGTATGATTCCGGGCTTTGAAGACGGTATCATCGGCATGAAAGCGGGTGAAGAGAAAGTTATTGATG
TCACTTTCCCAGAAGACTATCAAGCAGCGAACCTTGCAGGTAAAGCAGCAAAATTCAAGATCACAGTGAAGCAAGTTGAA
AAGCCAAAACTTCCTGAAATTGATGCTGAATTCCTAAAAATCTTTGGTATTTCTGAAGAAGATGGTGTTGAAAAGTTAAA
AGCTGATGTTCGCAAAAACATGGAGCGTGAAGTTCGTAACGGTCTTCGTAACCAAGTTAAGCAAGCAGCGTTTGATGCGC
TTGTTGCAGCGAATGAGATTGACGTACCATCAGCAATGGTTGCTCAGGAAATTGATCGTCAACGCGAGCAAATGATTCAG
CAATTTACTCAGCAGTTTGGTGGTGCGGGTGCGCAATCATTTGATAAGAGTATGCTTCCAGACGAATTGTTTAAAGAACA
AGCTGAAAAATCAGTAAAACTTGGTGTACTCGTAAGCAAAGTATTGGCTGATGCGAAACTTGAAGTCGATCAAGCACGTG
TTGATACTTATATCGACGAGATGGCATCTTCTTATGAAGATCCTACAGAAGTTGTTGATTACTTTAAAAATGATAAGCAA
CAACGTGCACAAATCGAAGCAGTTGTATTAGAAGATCAAGTGGTTGATCATATTCTTGCTGCAGCGAAAGTGACTGAAAC
AGAAGTTTCTTATGATGATTTATTGAAAGAACAACAAGCTCGTCAGGCTCGTTAA

Upstream 100 bases:

>100_bases
TCTAGGCTTTTTCACGATCTGTTTTGTATATCGGAAAAGTTGTCATATAATATAGCACTTTTAAAAATTGCTACTACTAA
CCTAATTTATCGAGGAAGCC

Downstream 100 bases:

>100_bases
TTCTGGATCGTAAAAAAGGCGCGTAAGCGCCTTTTTTTATTGATGTTGTGATTTTGTTATCTTTCAAACAATTCTGCTAT
CTTAAGTCGAAATAATCTGA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MQVTTEAVSGVARRLNVSVPTSRINEQFEARLKRTAKTAKINGFRPGKVPLSRVRSDFGPGIYQEVVNDIIRDTVFEAIQ
QEKINAVGMPNIEKVEHKDDALVYEATVEVYPEVEVKAFDTLEVERKAAEINDKDVDAMIENLQKQRQTWAVTKGMAKKD
MQVTFDFEGEVDGEKFEGGSAEDFKLVLGSGRMIPGFEDGIIGMKAGEEKVIDVTFPEDYQAANLAGKAAKFKITVKQVE
KPKLPEIDAEFLKIFGISEEDGVEKLKADVRKNMEREVRNGLRNQVKQAAFDALVAANEIDVPSAMVAQEIDRQREQMIQ
QFTQQFGGAGAQSFDKSMLPDELFKEQAEKSVKLGVLVSKVLADAKLEVDQARVDTYIDEMASSYEDPTEVVDYFKNDKQ
QRAQIEAVVLEDQVVDHILAAAKVTETEVSYDDLLKEQQARQAR

Sequences:

>Translated_444_residues
MQVTTEAVSGVARRLNVSVPTSRINEQFEARLKRTAKTAKINGFRPGKVPLSRVRSDFGPGIYQEVVNDIIRDTVFEAIQ
QEKINAVGMPNIEKVEHKDDALVYEATVEVYPEVEVKAFDTLEVERKAAEINDKDVDAMIENLQKQRQTWAVTKGMAKKD
MQVTFDFEGEVDGEKFEGGSAEDFKLVLGSGRMIPGFEDGIIGMKAGEEKVIDVTFPEDYQAANLAGKAAKFKITVKQVE
KPKLPEIDAEFLKIFGISEEDGVEKLKADVRKNMEREVRNGLRNQVKQAAFDALVAANEIDVPSAMVAQEIDRQREQMIQ
QFTQQFGGAGAQSFDKSMLPDELFKEQAEKSVKLGVLVSKVLADAKLEVDQARVDTYIDEMASSYEDPTEVVDYFKNDKQ
QRAQIEAVVLEDQVVDHILAAAKVTETEVSYDDLLKEQQARQAR
>Mature_444_residues
MQVTTEAVSGVARRLNVSVPTSRINEQFEARLKRTAKTAKINGFRPGKVPLSRVRSDFGPGIYQEVVNDIIRDTVFEAIQ
QEKINAVGMPNIEKVEHKDDALVYEATVEVYPEVEVKAFDTLEVERKAAEINDKDVDAMIENLQKQRQTWAVTKGMAKKD
MQVTFDFEGEVDGEKFEGGSAEDFKLVLGSGRMIPGFEDGIIGMKAGEEKVIDVTFPEDYQAANLAGKAAKFKITVKQVE
KPKLPEIDAEFLKIFGISEEDGVEKLKADVRKNMEREVRNGLRNQVKQAAFDALVAANEIDVPSAMVAQEIDRQREQMIQ
QFTQQFGGAGAQSFDKSMLPDELFKEQAEKSVKLGVLVSKVLADAKLEVDQARVDTYIDEMASSYEDPTEVVDYFKNDKQ
QRAQIEAVVLEDQVVDHILAAAKVTETEVSYDDLLKEQQARQAR

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=440, Percent_Identity=46.3636363636364, Blast_Score=390, Evalue=1e-109,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_ACIAD (Q6FEP9)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045281.1
- ProteinModelPortal:   Q6FEP9
- SMR:   Q6FEP9
- STRING:   Q6FEP9
- GeneID:   2880753
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0533
- NMPDR:   fig|62977.3.peg.1296
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   Q6FEP9
- ProtClustDB:   PRK01490
- BioCyc:   ASP62977:ACIAD0533-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PANTHER:   PTHR10516
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 49695; Mature: 49695

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVTTEAVSGVARRLNVSVPTSRINEQFEARLKRTAKTAKINGFRPGKVPLSRVRSDFGP
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCC
GIYQEVVNDIIRDTVFEAIQQEKINAVGMPNIEKVEHKDDALVYEATVEVYPEVEVKAFD
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEEEEEECCCCEEEEHH
TLEVERKAAEINDKDVDAMIENLQKQRQTWAVTKGMAKKDMQVTFDFEGEVDGEKFEGGS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCC
AEDFKLVLGSGRMIPGFEDGIIGMKAGEEKVIDVTFPEDYQAANLAGKAAKFKITVKQVE
CCCEEEEECCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCHHHHCCCCEEEEEEHHHCC
KPKLPEIDAEFLKIFGISEEDGVEKLKADVRKNMEREVRNGLRNQVKQAAFDALVAANEI
CCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DVPSAMVAQEIDRQREQMIQQFTQQFGGAGAQSFDKSMLPDELFKEQAEKSVKLGVLVSK
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VLADAKLEVDQARVDTYIDEMASSYEDPTEVVDYFKNDKQQRAQIEAVVLEDQVVDHILA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AAKVTETEVSYDDLLKEQQARQAR
HHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MQVTTEAVSGVARRLNVSVPTSRINEQFEARLKRTAKTAKINGFRPGKVPLSRVRSDFGP
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCC
GIYQEVVNDIIRDTVFEAIQQEKINAVGMPNIEKVEHKDDALVYEATVEVYPEVEVKAFD
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEEEEEECCCCEEEEHH
TLEVERKAAEINDKDVDAMIENLQKQRQTWAVTKGMAKKDMQVTFDFEGEVDGEKFEGGS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCC
AEDFKLVLGSGRMIPGFEDGIIGMKAGEEKVIDVTFPEDYQAANLAGKAAKFKITVKQVE
CCCEEEEECCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCHHHHCCCCEEEEEEHHHCC
KPKLPEIDAEFLKIFGISEEDGVEKLKADVRKNMEREVRNGLRNQVKQAAFDALVAANEI
CCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DVPSAMVAQEIDRQREQMIQQFTQQFGGAGAQSFDKSMLPDELFKEQAEKSVKLGVLVSK
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VLADAKLEVDQARVDTYIDEMASSYEDPTEVVDYFKNDKQQRAQIEAVVLEDQVVDHILA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AAKVTETEVSYDDLLKEQQARQAR
HHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA