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The map label for this gene is truA

Identifier: 50083718

GI number: 50083718

Start: 467810

End: 468625

Strand: Reverse

Name: truA

Synonym: ACIAD0474

Alternate gene names: 50083718

Gene position: 468625-467810 (Counterclockwise)

Preceding gene: 50083719

Following gene: 50083716

Centisome position: 13.02

GC content: 46.69

Gene sequence:

>816_bases
ATGATGCAACGTTACGCAGTTGGAATTGAGTTTTGTGGTATCCGTTATCGGGGCTGGCAGACTCAACAGGCTGGCGTACC
CAGCATTCAGGAAACCATTGAGAAAGTACTCAGCAAGATTGCCGATGAGCCCATTATTTTGCATGGTGCTGGACGAACCG
ATGCTGGTGTGCATGCCACCAATATGGTGGCGCATTTTGACACCAATGCAATTCGCCCTCAACGCGGCTGGCTCATGGGT
GCCAATAGTCAACTTCCCAAAGACATTTCTATCCAGTGGATTAAGGAAATGAATACGGATTTCCATGCACGCTTTAAAGC
CACGGCCCGTCGCTATCGCTATGTAGTTTACAATACGCTGAATCGACCTGCCCTGTTACACAAGCAGGTAACACATGTTT
ATCAGACACTTGATGTCGATAAAATGATGCTGGCCGCAAGAAAATTTGAAGGTACACACAACTTTGAAACGTTTCGTGCA
GCATCCTGCCAATCCAGTCAGCCAGTACGACACTTAAGCCATTGTCGTTTAACCCGACATGGACGTTATCTGGTACTGGA
CATTCAGGCAGATGGTTTTTTACACCATATGGTTCGTAATATCATGGGCTGCTTGCTCGAAATTGGTCAAGGCTGTTATG
AAATTGAACATATTGATACCATGTTTGCTGCTCAAGACCGCAAAGCCGCGGGAGTGACAGCGCCAGCGGACGGATTGTAT
TTTATTCAGGCCTATTATCCTGAGCATTTTGAGTTGCCTCAGCATCCACTTGGCCCACATTGGCTCAATTTACCCGATGA
GATACCCAATATTTGA

Upstream 100 bases:

>100_bases
CGATCTATATGCCAAACTTTTACAGCTTTATTTAAAATACGCCACGGCCGAACAATGGCAACAGCACTGGTATGATTATT
AATAAATACATTGAGGTTAA

Downstream 100 bases:

>100_bases
TTAAACACATAATTAAGCTTCTATAAAAAATGCATCTTTCGATGCATTTTTTTTCATTTAACGTTGTTTACGCTTACGAT
ACTCAACAGGGGTTTCGTTG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MMQRYAVGIEFCGIRYRGWQTQQAGVPSIQETIEKVLSKIADEPIILHGAGRTDAGVHATNMVAHFDTNAIRPQRGWLMG
ANSQLPKDISIQWIKEMNTDFHARFKATARRYRYVVYNTLNRPALLHKQVTHVYQTLDVDKMMLAARKFEGTHNFETFRA
ASCQSSQPVRHLSHCRLTRHGRYLVLDIQADGFLHHMVRNIMGCLLEIGQGCYEIEHIDTMFAAQDRKAAGVTAPADGLY
FIQAYYPEHFELPQHPLGPHWLNLPDEIPNI

Sequences:

>Translated_271_residues
MMQRYAVGIEFCGIRYRGWQTQQAGVPSIQETIEKVLSKIADEPIILHGAGRTDAGVHATNMVAHFDTNAIRPQRGWLMG
ANSQLPKDISIQWIKEMNTDFHARFKATARRYRYVVYNTLNRPALLHKQVTHVYQTLDVDKMMLAARKFEGTHNFETFRA
ASCQSSQPVRHLSHCRLTRHGRYLVLDIQADGFLHHMVRNIMGCLLEIGQGCYEIEHIDTMFAAQDRKAAGVTAPADGLY
FIQAYYPEHFELPQHPLGPHWLNLPDEIPNI
>Mature_271_residues
MMQRYAVGIEFCGIRYRGWQTQQAGVPSIQETIEKVLSKIADEPIILHGAGRTDAGVHATNMVAHFDTNAIRPQRGWLMG
ANSQLPKDISIQWIKEMNTDFHARFKATARRYRYVVYNTLNRPALLHKQVTHVYQTLDVDKMMLAARKFEGTHNFETFRA
ASCQSSQPVRHLSHCRLTRHGRYLVLDIQADGFLHHMVRNIMGCLLEIGQGCYEIEHIDTMFAAQDRKAAGVTAPADGLY
FIQAYYPEHFELPQHPLGPHWLNLPDEIPNI

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=268, Percent_Identity=28.3582089552239, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1788657, Length=259, Percent_Identity=49.8069498069498, Blast_Score=275, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_ACIAD (Q6FEV2)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045228.1
- ProteinModelPortal:   Q6FEV2
- SMR:   Q6FEV2
- STRING:   Q6FEV2
- GeneID:   2880741
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0474
- NMPDR:   fig|62977.3.peg.1188
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   Q6FEV2
- ProtClustDB:   PRK00021
- BioCyc:   ASP62977:ACIAD0474-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 31008; Mature: 31008

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: ACT_SITE 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQRYAVGIEFCGIRYRGWQTQQAGVPSIQETIEKVLSKIADEPIILHGAGRTDAGVHAT
CCCCEEECEEEECEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHH
NMVAHFDTNAIRPQRGWLMGANSQLPKDISIQWIKEMNTDFHARFKATARRYRYVVYNTL
HEEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHEEEEEEECC
NRPALLHKQVTHVYQTLDVDKMMLAARKFEGTHNFETFRAASCQSSQPVRHLSHCRLTRH
CCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCC
GRYLVLDIQADGFLHHMVRNIMGCLLEIGQGCYEIEHIDTMFAAQDRKAAGVTAPADGLY
CCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCEE
FIQAYYPEHFELPQHPLGPHWLNLPDEIPNI
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMQRYAVGIEFCGIRYRGWQTQQAGVPSIQETIEKVLSKIADEPIILHGAGRTDAGVHAT
CCCCEEECEEEECEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHH
NMVAHFDTNAIRPQRGWLMGANSQLPKDISIQWIKEMNTDFHARFKATARRYRYVVYNTL
HEEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHEEEEEEECC
NRPALLHKQVTHVYQTLDVDKMMLAARKFEGTHNFETFRAASCQSSQPVRHLSHCRLTRH
CCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCC
GRYLVLDIQADGFLHHMVRNIMGCLLEIGQGCYEIEHIDTMFAAQDRKAAGVTAPADGLY
CCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCEE
FIQAYYPEHFELPQHPLGPHWLNLPDEIPNI
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA