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The map label for this gene is ansA [H]

Identifier: 50083719

GI number: 50083719

Start: 468644

End: 469609

Strand: Reverse

Name: ansA [H]

Synonym: ACIAD0476

Alternate gene names: 50083719

Gene position: 469609-468644 (Counterclockwise)

Preceding gene: 50083720

Following gene: 50083718

Centisome position: 13.05

GC content: 44.51

Gene sequence:

>966_bases
ATGAATAAAATTGCCTTAATTTACATGGGTGGAACCTTTGGGTGTGTTGGCGAGCCACTGGCTCCAATGCCTTTTCAGAC
ATTTAAGCCCCAGTTAGAGCGAATTATTCCACCACAATTCATAGTAAGTTGCTTTGAAGCACCATCTGTTAAAGACAGCA
GTGCTTGTACAGCTCAAGATTGGCTGTTGCTCATTCAAGAAATTCAACGTCTGCAACTTGAAGGATTTCATCGTTTTGTC
GTGATTCATGGTACAGATACCATGAGCTATGCAGCTGCAACGCTGGCACGTTTTTTAGGTAACAGTTGTCATGTAGTCAT
CACAGGTAGTCAGTATCCGCTACTCAATACAGCAGGCGACAACACGCGTGAATTTACTGATGCCATCGACAATCTGTATC
TGGCCTTAGAACAGGTTCAAGCGTTGAATCCTGGTACCTATCTGGCGTTTCACCATCAGGTCTTTCATGCTCAAACCGCG
CTAAAGAGCCATACTACAGAACTGGATGCTTTTTCAGGTATCGATGCAACCCAAGAATGCCGTACCAGCAGCACAGCCAC
GATTGTACATACTGAAGAGATTCAACGTAGCGCAGCACTTTCAGTCGTCAATCTAATGATGCAACCCATTGCGATGCATC
AGTTGGCACAACAACTGCAAAATATCTTAACTACTGCTCCGCATTTTTTAGTTATCCAAGGATTTGGCACAGGAAATCTG
GCTGTAAACGCCGATATTCTTGCCATCTTCCAAAAACTGCGTGAGCAAGGCTGTCTGGTGATTCTTACCACACAAGTCAC
ATTCGGGCAGATTGATCAGCGCTATGCGATTAGCGACTGGGTCAATACGGCTGGTATTGTCACTCATACAGGTCATGGGC
ATGCCGATCTATATGCCAAACTTTTACAGCTTTATTTAAAATACGCCACGGCCGAACAATGGCAACAGCACTGGTATGAT
TATTAA

Upstream 100 bases:

>100_bases
GCGCTCAACAGCTTGAACTCTCAAAAAAACTGCTAAATCGAATGGCATCTTAAAAAATATCGTTCTAACATAAGCAGTCA
GTTTGGACTGCTTTTTAGTT

Downstream 100 bases:

>100_bases
TAAATACATTGAGGTTAAATGATGCAACGTTACGCAGTTGGAATTGAGTTTTGTGGTATCCGTTATCGGGGCTGGCAGAC
TCAACAGGCTGGCGTACCCA

Product: L-asparaginase I (AnsA)

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNKIALIYMGGTFGCVGEPLAPMPFQTFKPQLERIIPPQFIVSCFEAPSVKDSSACTAQDWLLLIQEIQRLQLEGFHRFV
VIHGTDTMSYAAATLARFLGNSCHVVITGSQYPLLNTAGDNTREFTDAIDNLYLALEQVQALNPGTYLAFHHQVFHAQTA
LKSHTTELDAFSGIDATQECRTSSTATIVHTEEIQRSAALSVVNLMMQPIAMHQLAQQLQNILTTAPHFLVIQGFGTGNL
AVNADILAIFQKLREQGCLVILTTQVTFGQIDQRYAISDWVNTAGIVTHTGHGHADLYAKLLQLYLKYATAEQWQQHWYD
Y

Sequences:

>Translated_321_residues
MNKIALIYMGGTFGCVGEPLAPMPFQTFKPQLERIIPPQFIVSCFEAPSVKDSSACTAQDWLLLIQEIQRLQLEGFHRFV
VIHGTDTMSYAAATLARFLGNSCHVVITGSQYPLLNTAGDNTREFTDAIDNLYLALEQVQALNPGTYLAFHHQVFHAQTA
LKSHTTELDAFSGIDATQECRTSSTATIVHTEEIQRSAALSVVNLMMQPIAMHQLAQQLQNILTTAPHFLVIQGFGTGNL
AVNADILAIFQKLREQGCLVILTTQVTFGQIDQRYAISDWVNTAGIVTHTGHGHADLYAKLLQLYLKYATAEQWQQHWYD
Y
>Mature_321_residues
MNKIALIYMGGTFGCVGEPLAPMPFQTFKPQLERIIPPQFIVSCFEAPSVKDSSACTAQDWLLLIQEIQRLQLEGFHRFV
VIHGTDTMSYAAATLARFLGNSCHVVITGSQYPLLNTAGDNTREFTDAIDNLYLALEQVQALNPGTYLAFHHQVFHAQTA
LKSHTTELDAFSGIDATQECRTSSTATIVHTEEIQRSAALSVVNLMMQPIAMHQLAQQLQNILTTAPHFLVIQGFGTGNL
AVNADILAIFQKLREQGCLVILTTQVTFGQIDQRYAISDWVNTAGIVTHTGHGHADLYAKLLQLYLKYATAEQWQQHWYD
Y

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=255, Percent_Identity=27.843137254902, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788065, Length=330, Percent_Identity=26.3636363636364, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI193207372, Length=357, Percent_Identity=22.96918767507, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17558280, Length=357, Percent_Identity=22.96918767507, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17558278, Length=357, Percent_Identity=22.6890756302521, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193207374, Length=357, Percent_Identity=22.6890756302521, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24639609, Length=328, Percent_Identity=27.1341463414634, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24645530, Length=266, Percent_Identity=24.812030075188, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35702; Mature: 35702

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIALIYMGGTFGCVGEPLAPMPFQTFKPQLERIIPPQFIVSCFEAPSVKDSSACTAQD
CCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHH
WLLLIQEIQRLQLEGFHRFVVIHGTDTMSYAAATLARFLGNSCHVVITGSQYPLLNTAGD
HHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCC
NTREFTDAIDNLYLALEQVQALNPGTYLAFHHQVFHAQTALKSHTTELDAFSGIDATQEC
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHH
RTSSTATIVHTEEIQRSAALSVVNLMMQPIAMHQLAQQLQNILTTAPHFLVIQGFGTGNL
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCE
AVNADILAIFQKLREQGCLVILTTQVTFGQIDQRYAISDWVNTAGIVTHTGHGHADLYAK
EECHHHHHHHHHHHHCCCEEEEEECCCHHHCCCHHHHHHHHCCCCEEEECCCCHHHHHHH
LLQLYLKYATAEQWQQHWYDY
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKIALIYMGGTFGCVGEPLAPMPFQTFKPQLERIIPPQFIVSCFEAPSVKDSSACTAQD
CCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHH
WLLLIQEIQRLQLEGFHRFVVIHGTDTMSYAAATLARFLGNSCHVVITGSQYPLLNTAGD
HHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCC
NTREFTDAIDNLYLALEQVQALNPGTYLAFHHQVFHAQTALKSHTTELDAFSGIDATQEC
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHH
RTSSTATIVHTEEIQRSAALSVVNLMMQPIAMHQLAQQLQNILTTAPHFLVIQGFGTGNL
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCE
AVNADILAIFQKLREQGCLVILTTQVTFGQIDQRYAISDWVNTAGIVTHTGHGHADLYAK
EECHHHHHHHHHHHHCCCEEEEEECCCHHHCCCHHHHHHHHCCCCEEEECCCCHHHHHHH
LLQLYLKYATAEQWQQHWYDY
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]